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Conditional lethality of a yeast strain expressing human RHOA in place of RHO1 .
Conditional lethality of a yeast strain expressing human RHOA in place of RHO1 .

... ADP ribosylated to serve as a negative control; indeed, in such a strain (YOC724), the Rholp band was not observed (Fig. 4, lane 1). In cells expressing both the yeast RHOIN46s and human RHOA (YOC726), we found that levels of the RHOA protein do not decrease noticeably after 6 h of incubation at 37C ...
ATM and - Cancer Research
ATM and - Cancer Research

... is distinct from the former phosphatidylinositol 3⬘-kinase-like kinases because it does not appear to function in signal transduction damage response pathways but rather functions in DNA nonhomologous end joining, a pathway for DSB repair (Ref. 15; for a review, see Ref. 16). ...
fist: an Arabidopsis mutant with altered cell division planes and
fist: an Arabidopsis mutant with altered cell division planes and

... mutation that slows growth of cells from one cell layer can be compensated for by accelerated growth of wildtype cells (Stewart et al. 1974). Similarly, a maize mutant (tangled-1) with altered cell division orientation has essentially normal leaf shape (Smith et al. 1996). These data suggest that if ...
PDF
PDF

... 5 A). The overall appearance of the oogonia at both light- and electron-microscope levels is essentially similar to that of the primordial germ cell, the most characteristic feature being the highly lobed nucleus (Figs. 3B-D, 5 A, B, E). Fig. 3B illustrates two adjacent larval oogonia as seen in 1 / ...
Introduction to Microbiology
Introduction to Microbiology

... Microbiology is the study of organisms, called micro-organisms, that are too small to be perceived clearly by the unaided eye. If an object has a diameter of less than 0.1 mm, the eye cannot perceive it at all, and very little detail can be perceived in an object with a diameter of 1 mm. In general, ...
Yeast Mating Type
Yeast Mating Type

... diploidization of heterothallic yeast (Herskowitz and Oshima, 1981). This comes about as a result of some cells changing mating type during the growth of a homothallic strain. The first studies made use of spore-to-cell matings of ascospores of a homothallic strain with a or a cells of a heterothalli ...
A Dominant Negative Mutant of Cyclin-Dependent
A Dominant Negative Mutant of Cyclin-Dependent

... the way it occurs in yeast and animal cells. The endoreduplication cell cycle is a variant of the standard mitotic cell cycle in which there are recurrent S- and G-phases without M-phase (Edgar and Orr-Weaver, 2001). The mechanisms that enable cells to sequentially replicate chromosomes without prog ...
RNA Processing Bodies, Peroxisomes, Golgi
RNA Processing Bodies, Peroxisomes, Golgi

... contain several types of large RNA–protein complex, collectively termed RNA granules. In animal cells, RNA granules—including the RNA-processing body (P-body), stress granule, neuronal granule, and germ cell granule—are transported on microtubules (Hirokawa et al. 2009). In plants, RNA granules homo ...
Expression and function of cell adhesion molecules during neural
Expression and function of cell adhesion molecules during neural

... Expression and function of cell adhesion molecules during neural crest migration Sonja J. McKeown1, Adam S. Wallace and Richard B. Anderson Department of Anatomy and Neuroscience, University of Melbourne, 3010, VIC, ...
PDF - Potter Lab
PDF - Potter Lab

... be missed if mutations result in early homozygous lethality. The Drosophila adult eye facilitates such screens since it is an ordered array of ommatidia. A mutant clone induced by mitotic recombination can be directly compared with its marked wild-type twin-spot clone for changes in growth. Thus, in ...
Full Text  - The International Journal of Developmental Biology
Full Text - The International Journal of Developmental Biology

... 1q cell have centrosomal localization. 2d and 3d cells have slightly stronger expression than all other cells. (C) After the birth of 4d, all cells in the embryo have a low level of cytoplasmic expression. All cells have lost their centrosomal localization. 2q cells have slightly stronger diffuse ex ...
TGFβ/activin/nodal signaling is necessary for the maintenance of
TGFβ/activin/nodal signaling is necessary for the maintenance of

Protists PPT
Protists PPT

...  Have either one or two flagella  All reproduce asexually by mitosis  Some can also reproduce sexually – meiosis ...
Gene Section KSR1 (kinase suppressor of ras 1)
Gene Section KSR1 (kinase suppressor of ras 1)

... KSR1 is primarily recognized as a scaffold for the Raf/MEK/ERK kinase cascade. However, there are several published studies that sustain that KSR1 has catalytic activity. KSR1 is recognized as a pseudokinase, since mammalian KSR1 does not possess the lysine responsible for ATP orientation and hydrol ...
Monomeric alpha-catenin links cadherin to the actin cytoskeleton
Monomeric alpha-catenin links cadherin to the actin cytoskeleton

... and actin cytoskeleton through their cytoplasmic binding partners, the catenins1 . Elucidating the function of α-catenin, which operates at the interface of the cadherin–β-catenin complex and F-actin, is a major goal in the field7–9 . Studies on mammalian αE-catenin have given rise to two models for ...
Standard PDF - Wiley Online Library
Standard PDF - Wiley Online Library

... the size and number of chloroplasts vary between these tissues (Table 1). Bizonoplasts, giant cup-shaped unique chloroplasts with dimorphic ultrastructural organization in a single chloroplast, are located in dorsal epidermal cells: the upper zone is occupied by numerous layers of two to four stacke ...
Imaging Cell Wall Architecture in Single Zinnia
Imaging Cell Wall Architecture in Single Zinnia

... secondary wall thickenings serve as structural reinforcements that add strength and rigidity to prevent the collapse of the xylem under the high pressure created by fluid transport. During the final stages of transdifferentiation, TEs accumulate lignin in their secondary walls and undergo programmed ...
Live Cell Imaging Reveals Structural Associations
Live Cell Imaging Reveals Structural Associations

... Figure 2. Coalignment between Cortical AFs and MTs in Arabidopsis Hypocotyl Cells. (A) to (C) Dual-labeled GFP:FABD (A) and mCherry:TUA5 (B), and merge of A and B (C), observed in hypocotyl cells of 3-d-old etiolated seedlings. Carets enclose regions of co-occurrence of the two channels. (D) Selecte ...
Anti-OPN Monoclonal Antibodies as Probes of OPN Structure and
Anti-OPN Monoclonal Antibodies as Probes of OPN Structure and

... Christian C. Kazanecki, Josephine Cassella, Yao Li, Cassandra Louis, Tanya Gordonov, Esben S. Sørensen and David T. Denhardt ABSTRACT: OPN, a primarily secreted protein found in all body fluids, is post-translationally modified; there is also an intracellular form. It is functionally important in bo ...
On the origin, evolution, and nature of programmed cell
On the origin, evolution, and nature of programmed cell

... multicellular animals studied so far, including cnidaria, nematodes, insects, amphibians, birds and mammals.16,18,20,24,25,63,64 The evolutionary conservation of programmed cell death in the animal kingdom does not only involve its existence and role, but extends to some central aspects of its genet ...
Guidance of mesoderm cell migration in the Xenopus
Guidance of mesoderm cell migration in the Xenopus

... animal pole region (Nakatsuji and Johnson, 1983b). In Xenopus, migration in vitro is also directional, although only when aggregates of mesodermal cells are used to probe conditioned substratum, instead of single cells (Fig. 1A). Moreover, in the Xenopus embryo, anterior mesoderm cells extend locomo ...
9700/02 - StudyGuide.PK
9700/02 - StudyGuide.PK

... (b) Mature mammalian red blood cells have no nuclei. State one advantage and one disadvantage of this. advantage ........................................................................................................................ ...
Job Sharing in the Endomembrane System: Vacuolar
Job Sharing in the Endomembrane System: Vacuolar

... filled with a central vacuole containing mostly water and solutes that allow plants to maximize collection of solar energy and mineral nutrients by increasing the surface of their photosynthesizing and nutrient-absorbing organs at minimal cost. Besides being lowcost space fillers, vacuoles are the mai ...
Spatial and temporal regulation of DNA
Spatial and temporal regulation of DNA

... It was recently demonstrated that during the processes of germination, DNA fragmentation (about 200 bp laddering) occurred in aleurone cells. The appearance of DNA fragmentation started in the aleurone cells near the embryo and extended to the distal end of the grain during germination. This DNA fra ...
Identification and Characterization of Genes Required for
Identification and Characterization of Genes Required for

... compensatory growth rather than a localized regeneration ...
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Cell growth

The term cell growth is used in the contexts of cell development and cell division (reproduction). When used in the context of cell division, it refers to growth of cell populations, where a cell, known as the ""mother cell"", grows and divides to produce two ""daughter cells"" (M phase). When used in the context of cell development, the term refers to increase in cytoplasmic and organelle volume (G1 phase), as well as increase in genetic material (G2 phase) following the replication during S phase.
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