Subcellular localization of peroxidase in tomato fruit skin and the
... of either non-speci®c binding of symplastic peroxidase to the cell wall, or isozymes bound to membranes included in the `wall-bound' fraction. Therefore, subcellular localization of peroxidase in both immature and mature tomato fruit skins was studied. Immature fruits showed intense peroxidase activ ...
... of either non-speci®c binding of symplastic peroxidase to the cell wall, or isozymes bound to membranes included in the `wall-bound' fraction. Therefore, subcellular localization of peroxidase in both immature and mature tomato fruit skins was studied. Immature fruits showed intense peroxidase activ ...
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... 2000) and HRP-coupled secondary antibodies. Loading controls were detected with antibodies specific for -actin (AC15, Sigma) and -tubulin (N357, Amersham). NIH image software was used for quantification. ...
... 2000) and HRP-coupled secondary antibodies. Loading controls were detected with antibodies specific for -actin (AC15, Sigma) and -tubulin (N357, Amersham). NIH image software was used for quantification. ...
Daughter-Specific Transcription Factors Regulate Cell Size Control
... been considered to be a main regulator of the length of the G1 phase of the cell cycle, resulting in longer G1 in the smaller daughter cells. Our recent data confirmed this concept using quantitative time-lapse microscopy. However, it has been proposed that daughter-specific, Ace2-dependent repressi ...
... been considered to be a main regulator of the length of the G1 phase of the cell cycle, resulting in longer G1 in the smaller daughter cells. Our recent data confirmed this concept using quantitative time-lapse microscopy. However, it has been proposed that daughter-specific, Ace2-dependent repressi ...
Full Paper - Calcutta Research Group
... important person in the Guntur political circles and a prominent man at the Sri Venkateswarswami temple1 in Guntur. He was visiting the Collector at his camp office with two of his friends to seek permission for a celebration in the temple. After an hour’s wait, the Collector’s arrival was announced ...
... important person in the Guntur political circles and a prominent man at the Sri Venkateswarswami temple1 in Guntur. He was visiting the Collector at his camp office with two of his friends to seek permission for a celebration in the temple. After an hour’s wait, the Collector’s arrival was announced ...
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... commensally Escherichia coli varied between 22.20 and 23.24 Ln(CFU/100 ml); 22.22 and 23.13 Ln(CFU/100 ml); 22.35 and 23.32 Ln(CFU/100 ml) and between 22.17 and 22.93 Ln(CFU/100 ml) at the incubation temperatures 7°C, 23°C, 37°C and 44°C respectively. In the presence of extract concentration of Euca ...
... commensally Escherichia coli varied between 22.20 and 23.24 Ln(CFU/100 ml); 22.22 and 23.13 Ln(CFU/100 ml); 22.35 and 23.32 Ln(CFU/100 ml) and between 22.17 and 22.93 Ln(CFU/100 ml) at the incubation temperatures 7°C, 23°C, 37°C and 44°C respectively. In the presence of extract concentration of Euca ...
Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis
... to spinal curvatures in stat3 mutants (S2 Fig, S1–S3 Movies). In the first group, vertebrae with a straight vertebral body and perpendicular end plates display either gentle or sharp turns (S2B and S2C Fig). In the second group, abnormal vertebral morphology such as bent vertebral body and non-perpe ...
... to spinal curvatures in stat3 mutants (S2 Fig, S1–S3 Movies). In the first group, vertebrae with a straight vertebral body and perpendicular end plates display either gentle or sharp turns (S2B and S2C Fig). In the second group, abnormal vertebral morphology such as bent vertebral body and non-perpe ...
Innexin7a forms junctions that stabilize the basal
... expressing LifeAct-GFP (Fig. 4H,J,L,N). During basal membrane formation, a fine network of actin becomes visible at the base of the forming cells (Fig. 4H; supplementary material Movie 6). An overlay of a more apical view revealed enrichments of actin at the constrictions of the basal membrane in so ...
... expressing LifeAct-GFP (Fig. 4H,J,L,N). During basal membrane formation, a fine network of actin becomes visible at the base of the forming cells (Fig. 4H; supplementary material Movie 6). An overlay of a more apical view revealed enrichments of actin at the constrictions of the basal membrane in so ...
Regulative germ cell specification in axolotl embryos: a primitive trait
... urodele embryos PGCs do not develop from predetermined cells. Rather, urodele PGCs appear to develop from unspecialized mesodermal tissue that has responded to appropriate extracellular influences. Therefore the embryos of urodele and anuran amphibians have diverged in the way that they produce PGCs ...
... urodele embryos PGCs do not develop from predetermined cells. Rather, urodele PGCs appear to develop from unspecialized mesodermal tissue that has responded to appropriate extracellular influences. Therefore the embryos of urodele and anuran amphibians have diverged in the way that they produce PGCs ...
Extracellular matrix of the charophycean green algae
... to Meagan Harless who is always able to talk me down. ...
... to Meagan Harless who is always able to talk me down. ...
Developing Technology-Based Biology Assessments for Cell
... encouraged to focus on the cell as the basic unit of life. Two of the main subjects that are covered in cell biology include cellular necessities/functions (i.e., energy storage and waste removal required by all organisms) and cellular structure (i.e., the differences between organisms, such as plan ...
... encouraged to focus on the cell as the basic unit of life. Two of the main subjects that are covered in cell biology include cellular necessities/functions (i.e., energy storage and waste removal required by all organisms) and cellular structure (i.e., the differences between organisms, such as plan ...
Embryonic stem cell differentiation: emergence of a new era in
... programs generated in these two regions are different. Hematopoietic commitment is detected first in the yolk sac, where distinct blood islands appear, shortly following gastrulation (Moore and Metcalf 1970; Haar and Ackerman 1971; Palis et al. 1995, 1999). These blood islands consist of an inner cl ...
... programs generated in these two regions are different. Hematopoietic commitment is detected first in the yolk sac, where distinct blood islands appear, shortly following gastrulation (Moore and Metcalf 1970; Haar and Ackerman 1971; Palis et al. 1995, 1999). These blood islands consist of an inner cl ...
Control of organ shape - Development
... involving cell shape changes (Leptin and Grunewald, 1990; Sweeton et al., 1991). Two signaling molecules are known to regulate this process: folded gastrulation (fog) and concertina (cta). fog encodes a putative secreted molecule that signals via an unknown receptor to cta, which encodes a putative ...
... involving cell shape changes (Leptin and Grunewald, 1990; Sweeton et al., 1991). Two signaling molecules are known to regulate this process: folded gastrulation (fog) and concertina (cta). fog encodes a putative secreted molecule that signals via an unknown receptor to cta, which encodes a putative ...
Full Text - Global Science Books
... Both in monocots and in dicots, the primary root (PR) represents the first visible organ that can be recognized at the time of germination. The onset of PR development starts however much earlier during embryogenesis and in Arabidopsis these early developmental stages have been wellstudied (Mayer et ...
... Both in monocots and in dicots, the primary root (PR) represents the first visible organ that can be recognized at the time of germination. The onset of PR development starts however much earlier during embryogenesis and in Arabidopsis these early developmental stages have been wellstudied (Mayer et ...
An Isotype-specific Activator of Major Histocompatibility Complex
... process has taken advantage of the existence of both MHC class II CID patient- and experimentally derived mutant cell lines that do not express class II genes but can be induced to do so upon somatic cell fusion with a class II–positive cell line (6). Somatic cell fusion between the mutant cell line ...
... process has taken advantage of the existence of both MHC class II CID patient- and experimentally derived mutant cell lines that do not express class II genes but can be induced to do so upon somatic cell fusion with a class II–positive cell line (6). Somatic cell fusion between the mutant cell line ...
biology and medicine Embryonic stem cell differentiation
... programs generated in these two regions are different. Hematopoietic commitment is detected first in the yolk sac, where distinct blood islands appear, shortly following gastrulation (Moore and Metcalf 1970; Haar and Ackerman 1971; Palis et al. 1995, 1999). These blood islands consist of an inner cl ...
... programs generated in these two regions are different. Hematopoietic commitment is detected first in the yolk sac, where distinct blood islands appear, shortly following gastrulation (Moore and Metcalf 1970; Haar and Ackerman 1971; Palis et al. 1995, 1999). These blood islands consist of an inner cl ...
The Dynamic Changes of Tonoplasts in Guard
... dynamic change of the surface area in the guard cells (Lambrechts et al., 1992). All these results indicate that another membrane system may serve as a reservoir for the plasma membrane to support the rapid dynamic volume change of guard cells during stomatal movement. In spite of vacuolar importanc ...
... dynamic change of the surface area in the guard cells (Lambrechts et al., 1992). All these results indicate that another membrane system may serve as a reservoir for the plasma membrane to support the rapid dynamic volume change of guard cells during stomatal movement. In spite of vacuolar importanc ...
Stringent Response Changes Cell Membrane Permeability in
... lowest antibiotic concentration which inhibited bacterial growth. Carbohydrate permeability assays. Standard curves for each carbohydrate, 2-deoxy-D-glucose (NBC, 4024), D-sucrose (Sigma, S0389) and dextran T2000 (BDH, 38015) were plotted to correlate absorbance with sugar concentrations using a phe ...
... lowest antibiotic concentration which inhibited bacterial growth. Carbohydrate permeability assays. Standard curves for each carbohydrate, 2-deoxy-D-glucose (NBC, 4024), D-sucrose (Sigma, S0389) and dextran T2000 (BDH, 38015) were plotted to correlate absorbance with sugar concentrations using a phe ...
Unleashing the potential of the root hair cell as a
... also an excellent model to decipher plant cell regulatory networks in response to abiotic stresses. This is based on their primary role in water and nutrient uptake. To utilize full potential of this attractive single cell type as a model in root systems biology, root hairs must be evenly treated pr ...
... also an excellent model to decipher plant cell regulatory networks in response to abiotic stresses. This is based on their primary role in water and nutrient uptake. To utilize full potential of this attractive single cell type as a model in root systems biology, root hairs must be evenly treated pr ...
Serial endosymbiosis or singular event at the origin of eukaryotes?
... Francis Crick’s remarks in the late 1950s (Crick, 1958) on the hidden wealth of phenotypic information available from amino acid sequences, and the pioneering work in the early 1960s by Emile Zuckerkandl and Linus Pauling on molecular clocks, which compared the amino acid sequences of hemoglobin cha ...
... Francis Crick’s remarks in the late 1950s (Crick, 1958) on the hidden wealth of phenotypic information available from amino acid sequences, and the pioneering work in the early 1960s by Emile Zuckerkandl and Linus Pauling on molecular clocks, which compared the amino acid sequences of hemoglobin cha ...
Sphingolipids Containing Very-Long
... elongation in plants that show phenotypes that have been attributed to depletion of VLCFA in sphingolipids. These mutants include cer10 (Zheng et al., 2005), gurke/pasticcino3 (pas3; Baud et al., 2004), pas2 (Bach et al., 2008), and pas1 (Roudier et al., 2010). In all these mutants, the level of VLC ...
... elongation in plants that show phenotypes that have been attributed to depletion of VLCFA in sphingolipids. These mutants include cer10 (Zheng et al., 2005), gurke/pasticcino3 (pas3; Baud et al., 2004), pas2 (Bach et al., 2008), and pas1 (Roudier et al., 2010). In all these mutants, the level of VLC ...
Dynamics of clathrin-mediated endocytosis and its requirement for
... redundancy and incomplete knockdowns. We took advantage of the availability of clathrin and AP2 knockouts in Dictyostelium to investigate whether they are required for the observed cell surface dynamics of puncta. We expressed GFP-Ap2A1 in cells with either disrupted clathrin light chain (clc–) (Wan ...
... redundancy and incomplete knockdowns. We took advantage of the availability of clathrin and AP2 knockouts in Dictyostelium to investigate whether they are required for the observed cell surface dynamics of puncta. We expressed GFP-Ap2A1 in cells with either disrupted clathrin light chain (clc–) (Wan ...
Phosphatidylinositol 4-Phosphate Formation at ER Exit Sites
... from the ER, whereas the catalytically inactive mutant (Sac1C392S) had no effect (Figure 2G). The inhibitory effect was reproduced in morphological ER export assays. Sac1 quantitatively delayed the cytosol-dependent mobilization of VSV-Gts to VTCs as analyzed by IF in permeabilized VSV-Gts expressin ...
... from the ER, whereas the catalytically inactive mutant (Sac1C392S) had no effect (Figure 2G). The inhibitory effect was reproduced in morphological ER export assays. Sac1 quantitatively delayed the cytosol-dependent mobilization of VSV-Gts to VTCs as analyzed by IF in permeabilized VSV-Gts expressin ...
Lewis - Gene Ontology Consortium
... Molecules, cell components , organisms are independent continuants which have functions (these are dependent continuants), and these functions may be realized as an ...
... Molecules, cell components , organisms are independent continuants which have functions (these are dependent continuants), and these functions may be realized as an ...
Shared versus Specialized Glycinergic Spinal Interneurons in Axial
... elicit in both the free-swimming and fictive preparation with age, this developmental stage was chosen to ensure that larvae would routinely exhibit struggling. All fish were reared in an in-house laboratory facility. Electrophysiology. Larvae were anesthetized in a 0.02% solution of MS222 and then ...
... elicit in both the free-swimming and fictive preparation with age, this developmental stage was chosen to ensure that larvae would routinely exhibit struggling. All fish were reared in an in-house laboratory facility. Electrophysiology. Larvae were anesthetized in a 0.02% solution of MS222 and then ...