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ICAR ARS NET Previous Questions on Agricultural
... 71. Acid fast staining used for which bacteria? ...
... 71. Acid fast staining used for which bacteria? ...
The Size of It All
... • Gram Positive Cells: the cell walls have a thick layer of peptidoglycan and lower lipid content. The cell walls will dehydrate when exposed to the decolorizer retaining the crystal violetiodine complex. • Gram Negative Cells: the cell walls have a thinner peptidoglycan layer and a layer of ...
... • Gram Positive Cells: the cell walls have a thick layer of peptidoglycan and lower lipid content. The cell walls will dehydrate when exposed to the decolorizer retaining the crystal violetiodine complex. • Gram Negative Cells: the cell walls have a thinner peptidoglycan layer and a layer of ...
MLAB 1415- Hematology Keri Brophy-Martinez Hematopoiesis
... N/C ratio decreases with maturity as the nucleus decreases in size and the cytoplasm becomes more abundant ...
... N/C ratio decreases with maturity as the nucleus decreases in size and the cytoplasm becomes more abundant ...
microscopy technique-2
... Microorganisms must be fixed and stained prior examined under microscope to : - increase visibility (increase contrast) - accentuate specific morphological features - preserve them for future study Staining – coloring specimens with stain (dyes) ...
... Microorganisms must be fixed and stained prior examined under microscope to : - increase visibility (increase contrast) - accentuate specific morphological features - preserve them for future study Staining – coloring specimens with stain (dyes) ...
cell
... • Filaments, which are also part of the cytoskeleton and can be classified into two groups—actin filaments, which are flexible chains of actin molecules, and intermediate filaments, which are ropelike fibers formed from a variety of proteins—both groups providing tensile strength to withstand tensio ...
... • Filaments, which are also part of the cytoskeleton and can be classified into two groups—actin filaments, which are flexible chains of actin molecules, and intermediate filaments, which are ropelike fibers formed from a variety of proteins—both groups providing tensile strength to withstand tensio ...
Lab 4
... • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
... • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
Cell Diversity
... eukaryotic. The technician has several dyes she could use to stain the cells. Four of the dyes are described in the table to the right. Which dye could the technician use to determine whether the cells are prokaryotic or eukaryotic? ...
... eukaryotic. The technician has several dyes she could use to stain the cells. Four of the dyes are described in the table to the right. Which dye could the technician use to determine whether the cells are prokaryotic or eukaryotic? ...
Gram stain procedure
... Purpose: to differentiate between • G+ve bacteria and G-ve bacteria - This stain is a differential stain ,it consist of at least 3 reagents : ...
... Purpose: to differentiate between • G+ve bacteria and G-ve bacteria - This stain is a differential stain ,it consist of at least 3 reagents : ...
Indirect (negative) staining
... Indirect (negative) staining • To heighten the contrast between bacteria and the background, use is made of electron-dense "stains". • These are usually compounds of heavy metals of high atomic number, that serve to scatter the electrons from regions covered with the stain. • If bacterial particles ...
... Indirect (negative) staining • To heighten the contrast between bacteria and the background, use is made of electron-dense "stains". • These are usually compounds of heavy metals of high atomic number, that serve to scatter the electrons from regions covered with the stain. • If bacterial particles ...
Slide 1
... The crucial step in the staining process is the decolorizing step. The most accepted theory relies on the fact that the PPG is found in layers and the stain molecules are trapped within the many layers of the GP CW when they form the complex with the mordant Iodine ...
... The crucial step in the staining process is the decolorizing step. The most accepted theory relies on the fact that the PPG is found in layers and the stain molecules are trapped within the many layers of the GP CW when they form the complex with the mordant Iodine ...
Document
... Basic classification of bacteria is based on the cell wall structure. There are 2 main groups: Gram positive and Gram negative. Gram staining is a differential staining technique that provides an easy differentiation of bacteria into one of two groups. The staining technique, developed in the late 1 ...
... Basic classification of bacteria is based on the cell wall structure. There are 2 main groups: Gram positive and Gram negative. Gram staining is a differential staining technique that provides an easy differentiation of bacteria into one of two groups. The staining technique, developed in the late 1 ...
Mini-lesson on prokaryotic and eukaryotic cells
... because they were Streptococcus • You would have been able to see teeny tiny little balls on the slide • You did NOTHING WRONG. • The destaining step needs to be explained better in the protocol. • Next year we’ll try culturing the cells before making slides of them. Sorry for the frustration. (But ...
... because they were Streptococcus • You would have been able to see teeny tiny little balls on the slide • You did NOTHING WRONG. • The destaining step needs to be explained better in the protocol. • Next year we’ll try culturing the cells before making slides of them. Sorry for the frustration. (But ...
Topic 2: Microscopy and Staining Measurement of Microorganisms
... Once stained, spores strongly resist decolourisation, basis for most spore staining methods. Stains the spore, not cell body (cell washed free of dye). 2. Acid-fast staining: Some species, eg. Mycobacterium, do not readily bind simple stains. Due to high lipid content of a wall, we must use harsher ...
... Once stained, spores strongly resist decolourisation, basis for most spore staining methods. Stains the spore, not cell body (cell washed free of dye). 2. Acid-fast staining: Some species, eg. Mycobacterium, do not readily bind simple stains. Due to high lipid content of a wall, we must use harsher ...
DIFFERENTIAL STAINING, Part I
... Endospores are specialized structures produced by two bacterial genera, Bacillus and Clostridium. Endospores (so called because the spore is formed within the cell) are easily seen with a phase microscope as highly refractile bodies. However, these structures are impervious to most ordinary stains, ...
... Endospores are specialized structures produced by two bacterial genera, Bacillus and Clostridium. Endospores (so called because the spore is formed within the cell) are easily seen with a phase microscope as highly refractile bodies. However, these structures are impervious to most ordinary stains, ...
Reading GuideChapter3_7e
... The rest of the chapter sections (3.4-3.9) focus on the parts of the bacterial cell. As you read about these structures think about how these parts are similar to eukaryotic cell parts. Bacterial cells have some very important unique structures not found on eukaryotic cells such as: cell walls, endo ...
... The rest of the chapter sections (3.4-3.9) focus on the parts of the bacterial cell. As you read about these structures think about how these parts are similar to eukaryotic cell parts. Bacterial cells have some very important unique structures not found on eukaryotic cells such as: cell walls, endo ...
Chapter 3 Lecture Notes
... the pink cytoplasm doesn’t show through. Gram-negative cells, which have no stain because they were cleared in the differential step show up with pink cytoplasm. 1. Safranin is a counterstain, since it has a different color than the primary stain (crystal violet). f. The slide is then dried in bibul ...
... the pink cytoplasm doesn’t show through. Gram-negative cells, which have no stain because they were cleared in the differential step show up with pink cytoplasm. 1. Safranin is a counterstain, since it has a different color than the primary stain (crystal violet). f. The slide is then dried in bibul ...
BASIC TECHNIQUES Preparation of histological sections In order to
... components of cells a blue color. This characteristic is known as basophilia. Hematoxylin stains the nuclei of cells, and the RER of the cytoplasm. Eosin is an acidic dye that stains the basic components of the cells a reddish-pink color. This characteristic is known as acidophilia. Most of the cyto ...
... components of cells a blue color. This characteristic is known as basophilia. Hematoxylin stains the nuclei of cells, and the RER of the cytoplasm. Eosin is an acidic dye that stains the basic components of the cells a reddish-pink color. This characteristic is known as acidophilia. Most of the cyto ...
Microbial Tools
... ◦ An acidic dye is used ◦ Examples include nigrosin, congo red, india ink ◦ All cells appear clear with the background stained which reveals the shape, size, and arrangement ...
... ◦ An acidic dye is used ◦ Examples include nigrosin, congo red, india ink ◦ All cells appear clear with the background stained which reveals the shape, size, and arrangement ...
Microbiology 155
... •The resolving power of a lens is a numerical measure of the resolution that can be attained with that lens. • The smaller the distance between two points that can be resolved the stronger the power of the lens ...
... •The resolving power of a lens is a numerical measure of the resolution that can be attained with that lens. • The smaller the distance between two points that can be resolved the stronger the power of the lens ...
The Microscope: Window on an Invisible Realm
... o Gram (+) – thick peptidoglycan layer; resist decolorization; retain crystal violet; appear purple o (Gram (-) – thin peptidoglycan layer; thick lipopolysaccharide layer is dissolved by the decolorizer; cells will accept the counterstain – appear pink Best when performed on young, growing bacteri ...
... o Gram (+) – thick peptidoglycan layer; resist decolorization; retain crystal violet; appear purple o (Gram (-) – thin peptidoglycan layer; thick lipopolysaccharide layer is dissolved by the decolorizer; cells will accept the counterstain – appear pink Best when performed on young, growing bacteri ...
Table 01_001
... Schleiden and Schwann propose the cell theory, stating that the nucleated cell is the universal building block of plant and animal tissues. ...
... Schleiden and Schwann propose the cell theory, stating that the nucleated cell is the universal building block of plant and animal tissues. ...
Pre-Lesson 10: Bacterial Diseases I
... family, two species cause most of the infections in man. The two which cause most of the staph infections are: 1. ____________________ 2. ____________________ _________________________ is a normal part of the skin biota and is found on everyone. It normally does not cause disease. But, it can be an ...
... family, two species cause most of the infections in man. The two which cause most of the staph infections are: 1. ____________________ 2. ____________________ _________________________ is a normal part of the skin biota and is found on everyone. It normally does not cause disease. But, it can be an ...
Cold Acid Fast Stain Technique
... 4. Remove the blotting paper with forceps. Gently rinse with water and drain. 5. Decolorize with acid-alcohol (not the Gram Stain decolorizer) using 1 “healthy” squirt, followed by a gentle water rinse. 6. Counterstain with methylene blue for 1 minute. 7. Rinse, blot dry with bibulous paper and obse ...
... 4. Remove the blotting paper with forceps. Gently rinse with water and drain. 5. Decolorize with acid-alcohol (not the Gram Stain decolorizer) using 1 “healthy” squirt, followed by a gentle water rinse. 6. Counterstain with methylene blue for 1 minute. 7. Rinse, blot dry with bibulous paper and obse ...
Staining
![](https://commons.wikimedia.org/wiki/Special:FilePath/Microscope_with_stained_slide.jpg?width=300)
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.