Gram Stain Lab - EDHSGreenSea.net
... A. Differential stains are very useful in microbiology because they can be used to distinguish between groups of bacteria. The Gram stain is one of the most important steps in the characterization and identification of bacteria. The Gram stain separates bacteria into one of two groups: ...
... A. Differential stains are very useful in microbiology because they can be used to distinguish between groups of bacteria. The Gram stain is one of the most important steps in the characterization and identification of bacteria. The Gram stain separates bacteria into one of two groups: ...
Slide Master
... Each Slide Master has adjustable basis and check bead to maintain slides always in horizontal position. ...
... Each Slide Master has adjustable basis and check bead to maintain slides always in horizontal position. ...
Using Microscopes
... Then, each student will be assigned to discover the difference between plant and animal cells using a microscope. Setting Up a Wet Mount Slide: The teacher explains that a wet mount slide gets its name because it is wet with either stain or water. Stains are used to color parts of cells so they may ...
... Then, each student will be assigned to discover the difference between plant and animal cells using a microscope. Setting Up a Wet Mount Slide: The teacher explains that a wet mount slide gets its name because it is wet with either stain or water. Stains are used to color parts of cells so they may ...
Gram Stain
... • Kinyoun published ‘cold-staining’ method in 1915, which is commonly used today • Acid fast positive bacteria have fatty acid in their cell wall (mycolic acid) that makes them ‘acid fast’, i.e. do not decolorize with acid alcohol • Acid Fast Stain is a differential stain used to classify Acid fast ...
... • Kinyoun published ‘cold-staining’ method in 1915, which is commonly used today • Acid fast positive bacteria have fatty acid in their cell wall (mycolic acid) that makes them ‘acid fast’, i.e. do not decolorize with acid alcohol • Acid Fast Stain is a differential stain used to classify Acid fast ...
Gram Stain
... Gram Stain The Gram stain is the most common differential stain used in microbiology. Differential stains use more than one dye. The unique cellular components of the bacteria will determine how they will react to the different dyes. The Gram stain procedure has been basically unchanged since it was ...
... Gram Stain The Gram stain is the most common differential stain used in microbiology. Differential stains use more than one dye. The unique cellular components of the bacteria will determine how they will react to the different dyes. The Gram stain procedure has been basically unchanged since it was ...
(4) staining
... Staining A. Chemical Staining Properties (factors) - Chemical staining is influenced by several factors : 1-Concentration of stains . 2-Amount of ionization of the tissue chemical components. 3-pH of the environment in which the tissue chemical components and stain are found. 4-fixation treatment of ...
... Staining A. Chemical Staining Properties (factors) - Chemical staining is influenced by several factors : 1-Concentration of stains . 2-Amount of ionization of the tissue chemical components. 3-pH of the environment in which the tissue chemical components and stain are found. 4-fixation treatment of ...
The differential staining of Schizomycetes in tissue sections and in
... It would be very desirable if a similar method for the differential staining of other Schizomycetes were available which could be used routinely by the microscopist. &1y studies-as associate of Herr Dr. Friedhinder in the morgue of the city hospital in Berlin-have attempted to demonstrate cocci in t ...
... It would be very desirable if a similar method for the differential staining of other Schizomycetes were available which could be used routinely by the microscopist. &1y studies-as associate of Herr Dr. Friedhinder in the morgue of the city hospital in Berlin-have attempted to demonstrate cocci in t ...
Microbiology 20 Study Guide – Exam #1
... the structure and function of prokaryotic flagella o motility in peritrichous bacteria, spirochetes (axial filaments) the production and function of endospores the function of other prokaryotic structures o ribosomes, inclusions, chromatophores, glycocalyx, fimbriae, pili the characteristics of prok ...
... the structure and function of prokaryotic flagella o motility in peritrichous bacteria, spirochetes (axial filaments) the production and function of endospores the function of other prokaryotic structures o ribosomes, inclusions, chromatophores, glycocalyx, fimbriae, pili the characteristics of prok ...
Atypical Bacteria
... unicellular organisms classified? • complex system of classification – based on shape & size; oxygen, pH, and temperature requirements; laboratory characteristics, biochemical analyses, serology tests, nucleic acid and protein analysis techniques ...
... unicellular organisms classified? • complex system of classification – based on shape & size; oxygen, pH, and temperature requirements; laboratory characteristics, biochemical analyses, serology tests, nucleic acid and protein analysis techniques ...
Bacteria: An Overview
... Gram Staining - Results y Gram‐negative have extra layer of around cell wall y Violet stain bonds to the chemicals in this layer y Then gets washed off ...
... Gram Staining - Results y Gram‐negative have extra layer of around cell wall y Violet stain bonds to the chemicals in this layer y Then gets washed off ...
Jensara Study Guide
... 8. In week 1 you viewed Bacillus subtilis, Staphylococcus epidermidis, Nostoc, Saccharomyces cerevisiae, Spirillum volutans,, Amoeba proteus, Euglena and Paramecium. Know the description of these organisms (domain, shape, movement, special structures, etc.) Be able to recognize these organisms under ...
... 8. In week 1 you viewed Bacillus subtilis, Staphylococcus epidermidis, Nostoc, Saccharomyces cerevisiae, Spirillum volutans,, Amoeba proteus, Euglena and Paramecium. Know the description of these organisms (domain, shape, movement, special structures, etc.) Be able to recognize these organisms under ...
Gram Staining Cheek Cells From Your Mouth
... observe bacteria without adding color to them. Staining is the process by which color is added to bacteria so they can be viewed under the light microscopes in our classroom. Gram-staining is a specific staining technique invented in 1844 by the Danish bacteriologist, Hans Christian Gram. Gram-stain ...
... observe bacteria without adding color to them. Staining is the process by which color is added to bacteria so they can be viewed under the light microscopes in our classroom. Gram-staining is a specific staining technique invented in 1844 by the Danish bacteriologist, Hans Christian Gram. Gram-stain ...
Renal cases - Fagdyrlaegen
... Oropharyngeal contamination • Oropharynx contains nucleated squamous cells • The ‘striped’ organisms are Simonsiella, which are particular to the oropharynx • Neutrophilic inflammation also present – but interpreting any culture results will be difficult ...
... Oropharyngeal contamination • Oropharynx contains nucleated squamous cells • The ‘striped’ organisms are Simonsiella, which are particular to the oropharynx • Neutrophilic inflammation also present – but interpreting any culture results will be difficult ...
Lab 5: Using the negative stain to determine true morphology 10/18
... same is true of P. vulgaris and R. rubrum. One concern is that some of these organisms may also produce capsules. If so, then the presence of a capsule in the negative stain would make the organism appear larger than its actual size. We will have to perform a capsule stain with the organisms to dete ...
... same is true of P. vulgaris and R. rubrum. One concern is that some of these organisms may also produce capsules. If so, then the presence of a capsule in the negative stain would make the organism appear larger than its actual size. We will have to perform a capsule stain with the organisms to dete ...
KUMASI™ stabilized colloidal stain for polyacrylamide gels
... Compared to conventional Coomassie stains which generate chemically hazardous waste streams, the new generation of “ecologically friendly” Coomassie stains exhibit significantly decreased sensitivity. The solution is the KUMASI stabilized colloidal stain which can be reused at least four times, redu ...
... Compared to conventional Coomassie stains which generate chemically hazardous waste streams, the new generation of “ecologically friendly” Coomassie stains exhibit significantly decreased sensitivity. The solution is the KUMASI stabilized colloidal stain which can be reused at least four times, redu ...
the supra-vital staining of normal and malignant tissues with
... affinity for certain types of malignant cells. This does not appear consistent with the chemistry of the processes involved. In conclusion it may be said that as supra-vital stains the tetrazolium compounds are not particularly selective. On the other hand-they easily give good general staining and ...
... affinity for certain types of malignant cells. This does not appear consistent with the chemistry of the processes involved. In conclusion it may be said that as supra-vital stains the tetrazolium compounds are not particularly selective. On the other hand-they easily give good general staining and ...
PLAY
... Gram-positive bacteria tend to be killed by penicillin and detergents. Gram-negative bacteria are more resistant to ...
... Gram-positive bacteria tend to be killed by penicillin and detergents. Gram-negative bacteria are more resistant to ...
Cell-abrationLab
... specimen name, magnification power, and preparation (slice? whole? unstained? stained, with which one? etc.) 3. Publish your sketches on 8.5 in. x 11 in. white unlined paper. 4. Estimate cell dimensions (length and width in micrometers, µm) for your class' chosen specimen. Create a class data table ...
... specimen name, magnification power, and preparation (slice? whole? unstained? stained, with which one? etc.) 3. Publish your sketches on 8.5 in. x 11 in. white unlined paper. 4. Estimate cell dimensions (length and width in micrometers, µm) for your class' chosen specimen. Create a class data table ...
Microbiology Lab 1 Examination of Bacteria
... • There are several factors that could result in a grampositive organism staining gram-negatively: ...
... • There are several factors that could result in a grampositive organism staining gram-negatively: ...
Wet Mounts – Onion Skin Cells
... 1. Why do we stain specimens? Support your answer using the observations made when examining the onion cells with and without the iodine stain. Be specific by referring to the substances and organelles affected by the stain(s). (4 marks) 2. Stains increase contrast because certain structures absorb ...
... 1. Why do we stain specimens? Support your answer using the observations made when examining the onion cells with and without the iodine stain. Be specific by referring to the substances and organelles affected by the stain(s). (4 marks) 2. Stains increase contrast because certain structures absorb ...
Protocol Sheet 2a2014
... Krutzik PO, Nolan GP. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry (2003). ...
... Krutzik PO, Nolan GP. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry (2003). ...
Bacterial Classification and Identification
... bacteria respond poorly to the Gram stain. They resist the action of acid alcohol due to their complex lipids (acid-fastness ) • The complex glycolipid allows M. tuberculosis to survive the degradative effects of the phagolysosomes in unactivated macrophages. They also render the bacterium difficult ...
... bacteria respond poorly to the Gram stain. They resist the action of acid alcohol due to their complex lipids (acid-fastness ) • The complex glycolipid allows M. tuberculosis to survive the degradative effects of the phagolysosomes in unactivated macrophages. They also render the bacterium difficult ...
Document
... – The modified trichrome stain (chromotrope 2R) commonly is used to detect microsporidia in urine, stool, or mucus. – The rapid Gram chromotrope method can be performed more quickly (about 11 min) and combines the chromotrope method with a Gram-staining step. The spores stain dark violet, and the eq ...
... – The modified trichrome stain (chromotrope 2R) commonly is used to detect microsporidia in urine, stool, or mucus. – The rapid Gram chromotrope method can be performed more quickly (about 11 min) and combines the chromotrope method with a Gram-staining step. The spores stain dark violet, and the eq ...
Chapter 3: Microscopy Units of Measurement 1 µm = 10–6 m = 10–3
... nm can distinguish between two points ≥ 0.4 nm Shorter wavelengths of light provide greater resolution The refractive index is a measure of the light-bending ability of a medium. The light may bend in air so much that it misses the small high-magnification lens. Immersion oil is used to keep light ...
... nm can distinguish between two points ≥ 0.4 nm Shorter wavelengths of light provide greater resolution The refractive index is a measure of the light-bending ability of a medium. The light may bend in air so much that it misses the small high-magnification lens. Immersion oil is used to keep light ...
Staining
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.