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Answers to problem sets 1 to 3
... 6. Why are chemical stains required for visualizing cells and tissues with the basic light microscope? What advantage does fluorescent microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy? What advantage does confocal microscopy provide in comparison to ...
... 6. Why are chemical stains required for visualizing cells and tissues with the basic light microscope? What advantage does fluorescent microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy? What advantage does confocal microscopy provide in comparison to ...
Bacteria and Viruses
... • Gram negative appears pink or red. These have less peptidoglycan, which does not hold the violet dye. • After the violet stain, they are rinsed in a red dye. The gram negative pick up only the second color. ...
... • Gram negative appears pink or red. These have less peptidoglycan, which does not hold the violet dye. • After the violet stain, they are rinsed in a red dye. The gram negative pick up only the second color. ...
Product information: SiR-tubulin Kit (CY-SC002)
... microtubule dynamics are critical, we recommend to keep the concentration of SiR-tubulin equal or below 100 nM. For other purposes, using 1 M SiR-tubulin for staining is recommended. Prepare 1 mM stock solution. Dissolve the content of the vial of SiR-tubulin in 50 μL of anhydrous DMSO to make a 1 ...
... microtubule dynamics are critical, we recommend to keep the concentration of SiR-tubulin equal or below 100 nM. For other purposes, using 1 M SiR-tubulin for staining is recommended. Prepare 1 mM stock solution. Dissolve the content of the vial of SiR-tubulin in 50 μL of anhydrous DMSO to make a 1 ...
L4 Evaluation of plant drugs
... • Some plants contain so much oil that it needs to be removed to see other structures ...
... • Some plants contain so much oil that it needs to be removed to see other structures ...
Chapter 3: Concepts and Tools for Studying Microorganisms
... • Light microscopes usually have at least 3 lenses: low-power, high-power, and oil-immersion • The lens system must have high resolving power to see the specimen clearly Staining Techniques Provide Contrast • The simple stain technique involves flooding a prepared specimen with basic dye • The neg ...
... • Light microscopes usually have at least 3 lenses: low-power, high-power, and oil-immersion • The lens system must have high resolving power to see the specimen clearly Staining Techniques Provide Contrast • The simple stain technique involves flooding a prepared specimen with basic dye • The neg ...
Mycobacterium tuberculosis
... Cover the smear with acid-fast staining solution (Cabolfuchsin s in 5% Phenol ) Heat the slide gently for 10-15 min. (Keep the smear covered with staining solution while heating the slide and do not allow it to dry. Once you see that the staining solution that covers the smear is about to dry, add ...
... Cover the smear with acid-fast staining solution (Cabolfuchsin s in 5% Phenol ) Heat the slide gently for 10-15 min. (Keep the smear covered with staining solution while heating the slide and do not allow it to dry. Once you see that the staining solution that covers the smear is about to dry, add ...
0.61 x wavelength of light
... Bright-field Optics: Light Passing Straight Through the Sample •Most living cells are optically clear, so stains are essential to get bright field contrast •Preserving cell structure during staining and subsequent observation is essential, so cells must be treated with “fixatives” that make them st ...
... Bright-field Optics: Light Passing Straight Through the Sample •Most living cells are optically clear, so stains are essential to get bright field contrast •Preserving cell structure during staining and subsequent observation is essential, so cells must be treated with “fixatives” that make them st ...
Bacterial Morphology
... • Stains are salt compose of a positive or negative ions. Basic dyes, are positively ion charged where, in acidic dyes, negatively ion charged. • Bacteria cell is slightly negatively charged so attracting basic dyes (crystal violet, methylene blue, malachite green, and safranin). ...
... • Stains are salt compose of a positive or negative ions. Basic dyes, are positively ion charged where, in acidic dyes, negatively ion charged. • Bacteria cell is slightly negatively charged so attracting basic dyes (crystal violet, methylene blue, malachite green, and safranin). ...
Laboratory Midterm
... Carbohydrates: Benedict's test for reduced sugars, Lugol's iodine test for starch Proteins: Biuret test for proteins Lipids: Sudan dye test Diffusion, osmosis, and solubility Diffusion, osmosis, permeability Tonicity: hypotonic, isotonic and hypertonic Hemolysis, crenation Microscope Parts: ocular, ...
... Carbohydrates: Benedict's test for reduced sugars, Lugol's iodine test for starch Proteins: Biuret test for proteins Lipids: Sudan dye test Diffusion, osmosis, and solubility Diffusion, osmosis, permeability Tonicity: hypotonic, isotonic and hypertonic Hemolysis, crenation Microscope Parts: ocular, ...
NUCLEAR AND CYTOPLASMIC STAINING
... The cytoskeleton (also CSK) is a cellular "scaffolding" or "skeleton" contained within the cytoplasm and is made out of protein. The cytoskeleton is present in all cells; it was once thought to be uni ...
... The cytoskeleton (also CSK) is a cellular "scaffolding" or "skeleton" contained within the cytoplasm and is made out of protein. The cytoskeleton is present in all cells; it was once thought to be uni ...
Gram stain reagents - Bakersfield College
... 2. list reagents, functions and steps of a Gram stain. 3. evaluate a Gram stain reaction quality and troubleshoot causes of Gram staining problems. 4. describe the Gram stain reaction, cell shape and arrangement of S. aureus, E. coli and Bacillus sp. 5. interpret unknown slides for Gram stain reacti ...
... 2. list reagents, functions and steps of a Gram stain. 3. evaluate a Gram stain reaction quality and troubleshoot causes of Gram staining problems. 4. describe the Gram stain reaction, cell shape and arrangement of S. aureus, E. coli and Bacillus sp. 5. interpret unknown slides for Gram stain reacti ...
ch_04 - studylib.net
... The colored portion of a dye, known as the chromophore, typically binds to chemicals via covalent, ionic, or hydrogen bonds. Anionic chromophores called acidic dyes or cationic chromophores known as basic dyes are used to stain different portions of an organism to aid viewing and identification. Sim ...
... The colored portion of a dye, known as the chromophore, typically binds to chemicals via covalent, ionic, or hydrogen bonds. Anionic chromophores called acidic dyes or cationic chromophores known as basic dyes are used to stain different portions of an organism to aid viewing and identification. Sim ...
Laboratory 7
... bacterial colonies. • Can be used to isolate colonies in pure or mixed cultures ...
... bacterial colonies. • Can be used to isolate colonies in pure or mixed cultures ...
a8d8a08cf7cae2b
... Human prostate gland Show end secretory parts of main of prostatic gland Strom composed from smooth muscle cells and c.t Prostatic concentration in the end secrtory parts of gland Stain : h&e ...
... Human prostate gland Show end secretory parts of main of prostatic gland Strom composed from smooth muscle cells and c.t Prostatic concentration in the end secrtory parts of gland Stain : h&e ...
2 common staining technique
... into various groups or types. Both the techniques allow the observation of cell morphology, or shape, but differential staining usually provides more information about the characteristics of the cell wall (Thickness). Gram staining (or Gram’s method) is an emprical method of differentiating bacteria ...
... into various groups or types. Both the techniques allow the observation of cell morphology, or shape, but differential staining usually provides more information about the characteristics of the cell wall (Thickness). Gram staining (or Gram’s method) is an emprical method of differentiating bacteria ...
Karyotyping - Cell Migration Gateway
... dish) with media containing 10 µg/ml colchicin and grow cells for 6-12 hours for ES cells, 12-18 hours for other cells. (stock is 50 mg/mL in EtOH, protect from light, store at -20oC) 50mg/ml is 5,000x, so 2µL stock for 10ml media. 2) Rinse with PBS, trypsinize for 5 min. (make sure the cells are in ...
... dish) with media containing 10 µg/ml colchicin and grow cells for 6-12 hours for ES cells, 12-18 hours for other cells. (stock is 50 mg/mL in EtOH, protect from light, store at -20oC) 50mg/ml is 5,000x, so 2µL stock for 10ml media. 2) Rinse with PBS, trypsinize for 5 min. (make sure the cells are in ...
Product: Rabbit Polyclonal IgG - Isotype Control Purified Cat. Ref
... surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections. Reactivity: This monoclonal antibody does not react with any antigen. It is a purified IgG. It was purified from serum collected from a rabbit prior to i ...
... surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections. Reactivity: This monoclonal antibody does not react with any antigen. It is a purified IgG. It was purified from serum collected from a rabbit prior to i ...
Chlamidya trachomatos
... in water supply for face washing and sanitation . • Environmental changes is also important in preventing trachoma. Done by limiting number of flies, discouraging people from sleeping close to their livestock, and encouraging villagers to regularly collect and burn trash. • Detection and treatment i ...
... in water supply for face washing and sanitation . • Environmental changes is also important in preventing trachoma. Done by limiting number of flies, discouraging people from sleeping close to their livestock, and encouraging villagers to regularly collect and burn trash. • Detection and treatment i ...
Microbiology exam # 1
... c) presence of certain metals d) presence of certain organic cofactors e) presence of membranes 14) Which of the following are true about Archaea (4) a) they are prokaryotes b) they lack peptidoglycan in their cell walls c) some are thermoacidophiles: others are extreme halophiles d) evidence sugges ...
... c) presence of certain metals d) presence of certain organic cofactors e) presence of membranes 14) Which of the following are true about Archaea (4) a) they are prokaryotes b) they lack peptidoglycan in their cell walls c) some are thermoacidophiles: others are extreme halophiles d) evidence sugges ...
Microbiology: A Systems Approach, 2nd ed.
... Examples include malachite green, crystal violet, basic fuchsin, and safranin All cells appear the same color but can reveal shape, size, and arrangement ...
... Examples include malachite green, crystal violet, basic fuchsin, and safranin All cells appear the same color but can reveal shape, size, and arrangement ...
Acid-fast stain
... fastness to the bacterium. Acid-fast bacteria, of which there are very few---the major genus Mycobacterium, have a high concentration of mycolic acid, a lipid, in their walls. Although difficult to stain, once the stain goes into the wall, the cell will not de-stain or decolorize easily. The ability ...
... fastness to the bacterium. Acid-fast bacteria, of which there are very few---the major genus Mycobacterium, have a high concentration of mycolic acid, a lipid, in their walls. Although difficult to stain, once the stain goes into the wall, the cell will not de-stain or decolorize easily. The ability ...
Keith King: Chapter 3
... Preparation of Specimens for Light Microscopy Smear: A thin film of a solution of microbes on a slide. ...
... Preparation of Specimens for Light Microscopy Smear: A thin film of a solution of microbes on a slide. ...
Staining
![](https://commons.wikimedia.org/wiki/Special:FilePath/Microscope_with_stained_slide.jpg?width=300)
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.