Staining of Blood parasites other than malaria parasites
... arise from a small granule - the kinetoplast. The length and position of the trypanosome’s flagellum is variable. In trypanosomes from the blood of a host the flagellum originates near the posterior end of the cell and passes forward over the cell surface, its sheath is expanded and forms a wavy fla ...
... arise from a small granule - the kinetoplast. The length and position of the trypanosome’s flagellum is variable. In trypanosomes from the blood of a host the flagellum originates near the posterior end of the cell and passes forward over the cell surface, its sheath is expanded and forms a wavy fla ...
Experiment 2 Microscopy: Simple staining, Gram stain and cell
... Experiment 2 Microscopy: Simple staining, Gram stain and cell fractionation 2.1 Introduction Most living microorganisms are generally colorless and almost invisible because of their lack of contrast with the water in which they may reside, staining is necessary in order to make them readily visible ...
... Experiment 2 Microscopy: Simple staining, Gram stain and cell fractionation 2.1 Introduction Most living microorganisms are generally colorless and almost invisible because of their lack of contrast with the water in which they may reside, staining is necessary in order to make them readily visible ...
Boya
... components of bacterial cells and stains the cells purple. Iodine (I – or I3 – ) interacts with CV+ and forms large complexes of crystal violet and iodine (CV – I) within the inner and outer layers of the cell. 3. Decolorizer is used next to remove the primary stain (crystal violet) from Gram(-) bac ...
... components of bacterial cells and stains the cells purple. Iodine (I – or I3 – ) interacts with CV+ and forms large complexes of crystal violet and iodine (CV – I) within the inner and outer layers of the cell. 3. Decolorizer is used next to remove the primary stain (crystal violet) from Gram(-) bac ...
Heat-Induced Apoptosis in Human Glioblastoma
... OBJECTIVE: Hyperthermia has been clinically applied to some types of brain tumors.However, the detailed mechanisms of this growth inhibition are not clear. The effect of mild hyperthermia on cultured human glioblastoma cell line, A172, was studied. METHODS: A172 cells were heat treated (43-44.5 degr ...
... OBJECTIVE: Hyperthermia has been clinically applied to some types of brain tumors.However, the detailed mechanisms of this growth inhibition are not clear. The effect of mild hyperthermia on cultured human glioblastoma cell line, A172, was studied. METHODS: A172 cells were heat treated (43-44.5 degr ...
Chapter 3
... – Loops and needles - compare uses – Stains • basic (positive ion like crystal violet) • Acidic (negative ions like India ink) ...
... – Loops and needles - compare uses – Stains • basic (positive ion like crystal violet) • Acidic (negative ions like India ink) ...
Pathophysiology
... The histotechnologist then cuts ribbonlike sections for placement on slides. The instrument used is called a microtome. A water bath is used for spreading the paraffin ribbons and sections are placed on microscope slides to be stained. Tissues are stained at the staining center. The basic stain ...
... The histotechnologist then cuts ribbonlike sections for placement on slides. The instrument used is called a microtome. A water bath is used for spreading the paraffin ribbons and sections are placed on microscope slides to be stained. Tissues are stained at the staining center. The basic stain ...
microbiology introduction
... -lactose fermentation produces acids, which lower the pH and encourages dye absorption by the colonies, which are now colored purpleblack and display "nucleated colonies“- colonies with dark centers -lactose non-fermenters may increase the pH by deamination of proteins which ensures that the dye is ...
... -lactose fermentation produces acids, which lower the pH and encourages dye absorption by the colonies, which are now colored purpleblack and display "nucleated colonies“- colonies with dark centers -lactose non-fermenters may increase the pH by deamination of proteins which ensures that the dye is ...
Preview Sample 2
... specimens. Electron microscopes (EM) use electrons, not light waves, as an illumination source to provide high magnification (5,000× to1,000,000×) and high resolution (0.5 nm). Specimens viewed through optical microscopes can be either alive or dead, depending on the type of specimen preparation, bu ...
... specimens. Electron microscopes (EM) use electrons, not light waves, as an illumination source to provide high magnification (5,000× to1,000,000×) and high resolution (0.5 nm). Specimens viewed through optical microscopes can be either alive or dead, depending on the type of specimen preparation, bu ...
part 1
... # most cells and extracellular material are completely colorless and to be studied microscopically sections must typically be stained ( dyed ) . # Cells components such as nucleic acids with a net negative charge (anionic) stain basic dyes and are termed basophilic BUT cationic components such as pr ...
... # most cells and extracellular material are completely colorless and to be studied microscopically sections must typically be stained ( dyed ) . # Cells components such as nucleic acids with a net negative charge (anionic) stain basic dyes and are termed basophilic BUT cationic components such as pr ...
Intracellular Cytokine staining protocol
... Any fluorochrome conjugated mAb will be suitable to detect an antigen expressed at high level. The PE conjugated mAb will be recommended to detect an antigen expressed at lower density. Positive cells will be more separate from negative cells using a PE conjugated mAb. The non-specific binding will ...
... Any fluorochrome conjugated mAb will be suitable to detect an antigen expressed at high level. The PE conjugated mAb will be recommended to detect an antigen expressed at lower density. Positive cells will be more separate from negative cells using a PE conjugated mAb. The non-specific binding will ...
Pure Culture - IRSC Biology Department
... 3 goals to a good smear Making sure the cells adhere to the slide- heat fix Insure that shrinkage of the cells does NOT occurdistorts the cells, give improper representation of the cells shape/size-done by air-drying your slide before heat-fix Prepare a thin smear- thick smears make it harder to ...
... 3 goals to a good smear Making sure the cells adhere to the slide- heat fix Insure that shrinkage of the cells does NOT occurdistorts the cells, give improper representation of the cells shape/size-done by air-drying your slide before heat-fix Prepare a thin smear- thick smears make it harder to ...
Foundations in Microbiology - Houston Community College System
... allow examination of characteristics of live cells: motility, shape, & arrangement • fixed mounts are made by drying & heating a film of specimen. This smear is stained using dyes to permit visualization of cells or cell parts. ...
... allow examination of characteristics of live cells: motility, shape, & arrangement • fixed mounts are made by drying & heating a film of specimen. This smear is stained using dyes to permit visualization of cells or cell parts. ...
HW - 1
... b. Bacteria that can exist in high pH environments c. Microbes normally found in food and food products d. Bacteria that live in and on our bodies that may have a symbiotic function 6. Which of the following is NOT a Prokaryote? a. Fungi b. Bacteria c. Archaebacteria d. The Bubonic Plague 7. What is ...
... b. Bacteria that can exist in high pH environments c. Microbes normally found in food and food products d. Bacteria that live in and on our bodies that may have a symbiotic function 6. Which of the following is NOT a Prokaryote? a. Fungi b. Bacteria c. Archaebacteria d. The Bubonic Plague 7. What is ...
Methods of Microbiology
... • Basic dye (positive) attracted to cell wall ( negative) • Crystal violet, methylene blue, safranin ...
... • Basic dye (positive) attracted to cell wall ( negative) • Crystal violet, methylene blue, safranin ...
Staining Bacteria
... Danish doctor ). He developed a new method to stain bacteria so they can be visible in specimen samples. • Differentiate bacteria into two large groups (the Gram Positive and the Gram negative) • Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's ...
... Danish doctor ). He developed a new method to stain bacteria so they can be visible in specimen samples. • Differentiate bacteria into two large groups (the Gram Positive and the Gram negative) • Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's ...
f Tools of the Laboratory: The 5 I's of culturing microbes Isolation
... allow examination of characteristics of live cells: motility, shape, & arrangement • fixed mounts are made by drying & heating a film of specimen. This smear is stained using dyes to permit visualization of cells or cell parts. ...
... allow examination of characteristics of live cells: motility, shape, & arrangement • fixed mounts are made by drying & heating a film of specimen. This smear is stained using dyes to permit visualization of cells or cell parts. ...
Staining
... Resolving power is the smallest distance between two particles at which we are able to distinguish them as two separate objects Resolving power for light microscopy is 0,2 mm. Magnification – 1000-1500 times ...
... Resolving power is the smallest distance between two particles at which we are able to distinguish them as two separate objects Resolving power for light microscopy is 0,2 mm. Magnification – 1000-1500 times ...
Karyokinesis and Cytokinesis in Micrococcus pyogenes var. aureus
... and conclusions are principally based on use of the Feulgen stain, Acid· Giemsa stain and electron microscopy. Recently a new technique for staining the bacterial nucleus, not involving acid hydrolysis or other drastic treatments, has been developed (2) . Nuclear stains of various aged cultures ot M ...
... and conclusions are principally based on use of the Feulgen stain, Acid· Giemsa stain and electron microscopy. Recently a new technique for staining the bacterial nucleus, not involving acid hydrolysis or other drastic treatments, has been developed (2) . Nuclear stains of various aged cultures ot M ...
No Slide Title
... their densities. The photos below show liver cells after gentle homogenization and liver cell organelles separated by differential centrifugation after homogenization in a Teflon-in-glass homogenizer. Special stains help test the purity of the fractions and identify organelles. When the homogenizer ...
... their densities. The photos below show liver cells after gentle homogenization and liver cell organelles separated by differential centrifugation after homogenization in a Teflon-in-glass homogenizer. Special stains help test the purity of the fractions and identify organelles. When the homogenizer ...
2 StainsInMicro
... bacterial cells against a dark field. This is the reverse of direct staining, which produces dark or colored cells against a white field. For this reason, acidic stains are also known as negative stains. The most common negative stains are india ink and nigrosin. Staining Singly or in Combination Wh ...
... bacterial cells against a dark field. This is the reverse of direct staining, which produces dark or colored cells against a white field. For this reason, acidic stains are also known as negative stains. The most common negative stains are india ink and nigrosin. Staining Singly or in Combination Wh ...
Object 15: Gram stain
... What is it? The Gram stain is one of the most important stains in the microbiology laboratory, forming the basis of the identification and classification of bacteria. It divides bacteria into two broad categories according to the properties of their cell walls. Gram-positive bacteria, such as MRSA, ...
... What is it? The Gram stain is one of the most important stains in the microbiology laboratory, forming the basis of the identification and classification of bacteria. It divides bacteria into two broad categories according to the properties of their cell walls. Gram-positive bacteria, such as MRSA, ...
Staining
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells, for instance), or organelles within individual cells.In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.Simple staining is staining with only one stain/dye. There are various kinds of multiple staining, many of which are examples of counterstaining, differential staining, or both, including double staining and triple staining. Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.