Ruditapes decussatus (Mollusca: Bivalvia)
... at room temperature, in the dark, for 3 d (Ray 1966). Gills were then removed from FTM, blotted on filter paper, transferred to a Petri dish with SFSW containing antibiotics (Penicillin G 500 units ml-' and streptomycin 500 mg ml-l) and chopped with a scalpel. After attachment of the zoosporangia to ...
... at room temperature, in the dark, for 3 d (Ray 1966). Gills were then removed from FTM, blotted on filter paper, transferred to a Petri dish with SFSW containing antibiotics (Penicillin G 500 units ml-' and streptomycin 500 mg ml-l) and chopped with a scalpel. After attachment of the zoosporangia to ...
Selenoprotein reactivity Elias Arnér
... These enzymes detoxify peroxides (ROOH) by a seleniumdependent redox cycling mechanism, involving Sec in the active site and consuming glutathione: ...
... These enzymes detoxify peroxides (ROOH) by a seleniumdependent redox cycling mechanism, involving Sec in the active site and consuming glutathione: ...
Sucrose and Invertase, an Uneasy Alliance
... Figure 3.1. Scheme showing the involvement of acid invertase in regulation of cellular hexose content. Fast exchanges are shown by thick arrows, and slow exchanges by thin arrows. Fru, fructose; glu, glucose; INV, invertase; TP, triose phosphate; *, symplastic export of sucrose; **, apoplastic expor ...
... Figure 3.1. Scheme showing the involvement of acid invertase in regulation of cellular hexose content. Fast exchanges are shown by thick arrows, and slow exchanges by thin arrows. Fru, fructose; glu, glucose; INV, invertase; TP, triose phosphate; *, symplastic export of sucrose; **, apoplastic expor ...
as a PDF
... made up of double-stranded DNA. Cleavage of these doublestranded border sequences requires VirD1 and VirD2 proteins, both in vivo (82, 99, 155, 369) and in vitro (281). In vitro, however, VirD2 protein alone can cleave a single-stranded T-DNA border sequence (154, 249). Cleavage of the 25-bp T-DNA b ...
... made up of double-stranded DNA. Cleavage of these doublestranded border sequences requires VirD1 and VirD2 proteins, both in vivo (82, 99, 155, 369) and in vitro (281). In vitro, however, VirD2 protein alone can cleave a single-stranded T-DNA border sequence (154, 249). Cleavage of the 25-bp T-DNA b ...
Receptors Distinct Parasite Molecules and Host Chemoattractant
... IL-10-sensitive pathway leading to IL-12 production and an IL10-insensitive pathway that culminates in CCL2 release. Effects of IFN-␥ on IL-12 production are Stat1 dependent, but IFN-␥-driven CCL2 release does not require Stat1 Signaling by IFN-␥ proceeds largely, but not completely, through intrace ...
... IL-10-sensitive pathway leading to IL-12 production and an IL10-insensitive pathway that culminates in CCL2 release. Effects of IFN-␥ on IL-12 production are Stat1 dependent, but IFN-␥-driven CCL2 release does not require Stat1 Signaling by IFN-␥ proceeds largely, but not completely, through intrace ...
Developmental Changes in the Subcellular Localization of Calretinin
... confocal microscopy. To our surprise, the subcellular location of calretinin within NM neurons changed as a function of developmental age. At E10, calretinin was expressed at low levels, in agreement with our biochemical analysis, and diffusely throughout the NM neuron, in agreement with other repor ...
... confocal microscopy. To our surprise, the subcellular location of calretinin within NM neurons changed as a function of developmental age. At E10, calretinin was expressed at low levels, in agreement with our biochemical analysis, and diffusely throughout the NM neuron, in agreement with other repor ...
(apoE) Transgenic Mice
... Gel filtration chromatography of astroc yte-conditioned media. Serumfree, astrocyte-conditioned media (see below) was concentrated (C entriplus-10; Amicon, Beverly, M A) ;50-fold before fractionation. One milliliter of concentrated astrocyte-conditioned media was fractionated by gel filtration chrom ...
... Gel filtration chromatography of astroc yte-conditioned media. Serumfree, astrocyte-conditioned media (see below) was concentrated (C entriplus-10; Amicon, Beverly, M A) ;50-fold before fractionation. One milliliter of concentrated astrocyte-conditioned media was fractionated by gel filtration chrom ...
Odd-paired controls frequency doubling in Drosophila segmentation
... 2004; El-Sherif & Levine 2016). Another major reason is that, in addition to directing the initial expression of the segment-polarity genes, pair-rule genes also cross-regulate each other. Pair-rule proteins and transcripts turn over extremely rapidly (Edgar et al. 1986; Nasiadka & Krause 1999), and ...
... 2004; El-Sherif & Levine 2016). Another major reason is that, in addition to directing the initial expression of the segment-polarity genes, pair-rule genes also cross-regulate each other. Pair-rule proteins and transcripts turn over extremely rapidly (Edgar et al. 1986; Nasiadka & Krause 1999), and ...
Involvement of Calcium Ion in the Stimulated Shoot Elongation of
... early period, but it stopped after 3 d of incubation. In the presence of 2 mM CaCl2, the anaerobic elongation was enhanced and continued at a higher rate until the sixth day of incubation. These results suggest that CaCl2 acts as a growth enhancer in arrowhead shoots. This action of CaCl2 on shoot e ...
... early period, but it stopped after 3 d of incubation. In the presence of 2 mM CaCl2, the anaerobic elongation was enhanced and continued at a higher rate until the sixth day of incubation. These results suggest that CaCl2 acts as a growth enhancer in arrowhead shoots. This action of CaCl2 on shoot e ...
Involvement of auxin in the arbuscular mycorrhizal symbiosis in tomato
... Several miRNAs interact with auxin signaling and among them miR393 that targets auxin receptors. We investigated the role of miR393 in AM root colonization. In Solanum lycopersicum (Solanaceae), Medicago truncatula (Fabaceae) and Oryza sativa (Poaceae), expression of the precursors of the miR393 was ...
... Several miRNAs interact with auxin signaling and among them miR393 that targets auxin receptors. We investigated the role of miR393 in AM root colonization. In Solanum lycopersicum (Solanaceae), Medicago truncatula (Fabaceae) and Oryza sativa (Poaceae), expression of the precursors of the miR393 was ...
Calcium regulates ricin transport to the Golgi apparatus
... MDCK II cells grown on coverslips were washed twice with buffer (14 mM NaCl, 2 mM CaCl2, 20 mM Hepes) and incubated with or without 1 µg/ml thapsigargin or 10 µM A23187 for 30 minutes at 37°C in the same buffer. The cells were then loaded with fluorescent calcium indicators by incubation with buffer ...
... MDCK II cells grown on coverslips were washed twice with buffer (14 mM NaCl, 2 mM CaCl2, 20 mM Hepes) and incubated with or without 1 µg/ml thapsigargin or 10 µM A23187 for 30 minutes at 37°C in the same buffer. The cells were then loaded with fluorescent calcium indicators by incubation with buffer ...
Accommodating the bacterial decoding release factor as an
... Through to the 1980s, the expectation was that a ribosomal protein would be the enzyme for peptide bond formation, and there were several prime candidates [10]. At this time, the fact that an extrinsic decoding protein might join the intrinsic ribosomal proteins to carry out the function of stop sig ...
... Through to the 1980s, the expectation was that a ribosomal protein would be the enzyme for peptide bond formation, and there were several prime candidates [10]. At this time, the fact that an extrinsic decoding protein might join the intrinsic ribosomal proteins to carry out the function of stop sig ...
PDF
... localization (Fig. 1A). Application of 2 ng/ml TGFβ1 resulted in the rapid nuclear translocation of GFP-Smad4 that peaked within 1 h and its cytoplasmic relocalization within 6 h (Warmflash et al., 2012). Thus, we searched for genes that could either prevent GFP-Smad4 nuclear localization at 1 h or ...
... localization (Fig. 1A). Application of 2 ng/ml TGFβ1 resulted in the rapid nuclear translocation of GFP-Smad4 that peaked within 1 h and its cytoplasmic relocalization within 6 h (Warmflash et al., 2012). Thus, we searched for genes that could either prevent GFP-Smad4 nuclear localization at 1 h or ...
Paracoccidioides brasiliensis Strain IVIC Pb9
... M-form colonies of the parental strain (Fig. 2). Reversion to the wild-type morphology was not observed. Y-form colonies on Sabouraud agar were brain-like and easily detachable from the agar surface, resembling those of the parental strain 1 v 1 c ~ b 9(Carbonell & Rodriguez, 1965). Strain rvrc~b140 ...
... M-form colonies of the parental strain (Fig. 2). Reversion to the wild-type morphology was not observed. Y-form colonies on Sabouraud agar were brain-like and easily detachable from the agar surface, resembling those of the parental strain 1 v 1 c ~ b 9(Carbonell & Rodriguez, 1965). Strain rvrc~b140 ...
Dynamic balance between vesicle transport and
... experimental data from the literature to model in depth the various SCPs in each type and how they interacted. We developed analytical solutions that allowed us to calculate the relative relationships between different SCPs in a type that is needed to achieve overall balance between microtubule grow ...
... experimental data from the literature to model in depth the various SCPs in each type and how they interacted. We developed analytical solutions that allowed us to calculate the relative relationships between different SCPs in a type that is needed to achieve overall balance between microtubule grow ...
Space to grow: interplay between growth and patterning in plant
... Combinatorial interactions between tissue polarity and growth result in the generation of diverse biological forms. Tissue polarity organizers are specific regions that anchor tissue polarity and define growth orientations [24]••. Principal orientations of specified growth are determined according t ...
... Combinatorial interactions between tissue polarity and growth result in the generation of diverse biological forms. Tissue polarity organizers are specific regions that anchor tissue polarity and define growth orientations [24]••. Principal orientations of specified growth are determined according t ...
Multiple Roles of the Cytoskeleton in Bacterial Autophagy
... indicate that septin cages assemble in certain pathways of actin polymerization, for example, actin polymerization by WASP family proteins (as occurs in the case of Shigella and M. marinum), and/or that a cytosolic source of membrane is required to facilitate septin assembly surrounding the bacteriu ...
... indicate that septin cages assemble in certain pathways of actin polymerization, for example, actin polymerization by WASP family proteins (as occurs in the case of Shigella and M. marinum), and/or that a cytosolic source of membrane is required to facilitate septin assembly surrounding the bacteriu ...
Full Article - PDF - Journal of Biotech Research
... chemotherapy. Although P. falciparum folate pathway enzymes and metabolites have been studied extensively, the structure and function of transporters that underpin folate salvage are lacking. Plasmodium falciparum 3D7 and Homo sapiens folate transporter protein sequences were queried by using BLAST ...
... chemotherapy. Although P. falciparum folate pathway enzymes and metabolites have been studied extensively, the structure and function of transporters that underpin folate salvage are lacking. Plasmodium falciparum 3D7 and Homo sapiens folate transporter protein sequences were queried by using BLAST ...
The neomuran origin of archaebacteria, the negibacterial root of the
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
The neomuran origin of archaebacteria, the negibacterial root of the
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
... mere bags of enzymes. Genes and enzymes are both fundamental, but play their vital roles as parts of highly organized growing and dividing cells. Their life depends on a mutualistic symbiosis of genes, catalysts, membranes and cell skeleton (Cavalier-Smith, 1987a, 1991a, b, 2001). Co-adaptation betw ...
Proteolytic and other metabolic pathways in lysosomes
... N-acetylhexosamines. The final links to the protein may be tence of a number of activator proteins that dramatically 0-glycosidic links to serine or threonine, or N-glycosidic enhance the action of certain of the enzymes on the lipid links to asparagine. The 0-glycosidic links are hydrolysed substra ...
... N-acetylhexosamines. The final links to the protein may be tence of a number of activator proteins that dramatically 0-glycosidic links to serine or threonine, or N-glycosidic enhance the action of certain of the enzymes on the lipid links to asparagine. The 0-glycosidic links are hydrolysed substra ...
REACTIVE OXYGEN SPECIES - Institute of Plant Sciences Paris
... during rearrangement of cell walls in roots, hypocotyls, or coleoptiles (40, 94, ...
... during rearrangement of cell walls in roots, hypocotyls, or coleoptiles (40, 94, ...
PDF + SI - Development - The Company of Biologists
... when either the tip cell is not specified or AMs are laser ablated, the anterior MTs are misshapen and extend too far into the anterior of the embryo. Since AMs are missing or severely malformed in org-1 mutant embryos (Schaub et al., 2012), a similar MT phenotype might have been expected. However, ...
... when either the tip cell is not specified or AMs are laser ablated, the anterior MTs are misshapen and extend too far into the anterior of the embryo. Since AMs are missing or severely malformed in org-1 mutant embryos (Schaub et al., 2012), a similar MT phenotype might have been expected. However, ...
Intermediary Metabolism and Life-History Trade
... from the other blocks. Lines were close to being pure breeding (i.e., producing 195% of the selected morph) when experiments were conducted, and they were the same lines used in previous studies of lipid metabolism and lifehistory evolution (Zera and Bottsford 2001; Zera and Cisper 2001; Zhao and Ze ...
... from the other blocks. Lines were close to being pure breeding (i.e., producing 195% of the selected morph) when experiments were conducted, and they were the same lines used in previous studies of lipid metabolism and lifehistory evolution (Zera and Bottsford 2001; Zera and Cisper 2001; Zhao and Ze ...
Wnt-7a maintains appropriate uterine patterning
... Accepted 8 June; published on WWW 21 July 1998 ...
... Accepted 8 June; published on WWW 21 July 1998 ...