J., Schornack, S., Spallek, T., Geldner, N., Chory
... Hoechst staining was repeatedly found in close proximity to the Hpa haustorium, as recently described (Caillaud et al., 2011). Evidently, at this stage of the infection, when no sporulation of Hpa has yet occurred, the host cells are already responding with substantial subcellular changes and theref ...
... Hoechst staining was repeatedly found in close proximity to the Hpa haustorium, as recently described (Caillaud et al., 2011). Evidently, at this stage of the infection, when no sporulation of Hpa has yet occurred, the host cells are already responding with substantial subcellular changes and theref ...
The anorexigenic cytokine ciliary neurotrophic factor stimulates
... CNTF and its receptor subunits are expressed in anorexigenic neurons of the ARC. We have demonstrated previously that CNTF was expressed in 90% of astrocytes and 75% of neurons in the rat ARC (57). Because CNTF administration decreases food intake, we addressed the possibility for the cytokine to be ...
... CNTF and its receptor subunits are expressed in anorexigenic neurons of the ARC. We have demonstrated previously that CNTF was expressed in 90% of astrocytes and 75% of neurons in the rat ARC (57). Because CNTF administration decreases food intake, we addressed the possibility for the cytokine to be ...
The neomuran origin of archaebacteria, the
... Actinobacteria (possibly from the new class Arabobacteria, from which eukaryotic cholesterol biosynthesis probably came). Replacement of eubacterial peptidoglycan by glycoproteins and adaptation to thermophily are the keys to neomuran origins. All 19 common neomuran character suites probably arose e ...
... Actinobacteria (possibly from the new class Arabobacteria, from which eukaryotic cholesterol biosynthesis probably came). Replacement of eubacterial peptidoglycan by glycoproteins and adaptation to thermophily are the keys to neomuran origins. All 19 common neomuran character suites probably arose e ...
ABSTRACT Title of Document:
... biofilm enables microbes to perform important cellular functions such as that of adaptation and persistence inside host. Interactions among community members are crucial for temporal and coordinated response and are often mediated by a process of population-dependent cell-cell communication known as ...
... biofilm enables microbes to perform important cellular functions such as that of adaptation and persistence inside host. Interactions among community members are crucial for temporal and coordinated response and are often mediated by a process of population-dependent cell-cell communication known as ...
Review Severe acute respiratory syndrome coronavirus entry as a
... HCoV-229E, porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus [66,76–79], but is not a receptor for HCoV-NL63 [80]. The receptor of HCoV-OC43 remains elusive, but the receptors of other group 2 viruses have been identified. For instance, those of murine hepatitis virus a ...
... HCoV-229E, porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus [66,76–79], but is not a receptor for HCoV-NL63 [80]. The receptor of HCoV-OC43 remains elusive, but the receptors of other group 2 viruses have been identified. For instance, those of murine hepatitis virus a ...
Lack of correlation between trehalose accumulation, cell viability
... incubation at 40 "C, since activity was increased by sixfold in the pmal-1 mutant but by only fourfold in the control strain. In contrast, addition of 10% ethanol to exponential-phase cells of wild-type and pmal-1 mutant strains growing on glucose caused a twofold reduction in the activity of this e ...
... incubation at 40 "C, since activity was increased by sixfold in the pmal-1 mutant but by only fourfold in the control strain. In contrast, addition of 10% ethanol to exponential-phase cells of wild-type and pmal-1 mutant strains growing on glucose caused a twofold reduction in the activity of this e ...
Structure of Mammalian AMPK and its regulation by ADP
... This observation indicates that it is AMP/ADP binding at the weaker of the two exchangeable sites, the one that does not bind NADH, that is responsible for protection against dephosphorylation. They also carried out co-crystallization of the regulatory fragment with one molar equivalent of ADP ...
... This observation indicates that it is AMP/ADP binding at the weaker of the two exchangeable sites, the one that does not bind NADH, that is responsible for protection against dephosphorylation. They also carried out co-crystallization of the regulatory fragment with one molar equivalent of ADP ...
Actin and Myosin Function in Directed Vacuole Movement during
... ited by the daughter cell and segregation structures are not observed (Fig. 1 b). The actin gene in this mutant encodes a truncated actin molecule that lacks the three NH2-terminal amino acids, and, aside from a slightly decreased ability to support invertase secretion, no phenotype had previously b ...
... ited by the daughter cell and segregation structures are not observed (Fig. 1 b). The actin gene in this mutant encodes a truncated actin molecule that lacks the three NH2-terminal amino acids, and, aside from a slightly decreased ability to support invertase secretion, no phenotype had previously b ...
Central Role of Voltage Gated Calcium Channels and
... neurons was shown to be dependent on the activities of LTCC [9437025] (see Neurotransmitters). Neuronal gene expression A transcription factor is a protein that acts as a regulator of gene expression. CREB (cAMP response element-binding) proteins are transcription factors which bind to cAMP response ...
... neurons was shown to be dependent on the activities of LTCC [9437025] (see Neurotransmitters). Neuronal gene expression A transcription factor is a protein that acts as a regulator of gene expression. CREB (cAMP response element-binding) proteins are transcription factors which bind to cAMP response ...
Improper chromosome synapsis is associated with
... Abstract The RecA homolog, RAD51, performs a central role in catalyzing the DNA strand exchange event of meiotic recombination. During meiosis, RAD51 complexes develop on pairing chromosomes and then most disappear upon synapsis. In the maize meiotic mutant desynaptic2 (dsy2), homologous chromosome ...
... Abstract The RecA homolog, RAD51, performs a central role in catalyzing the DNA strand exchange event of meiotic recombination. During meiosis, RAD51 complexes develop on pairing chromosomes and then most disappear upon synapsis. In the maize meiotic mutant desynaptic2 (dsy2), homologous chromosome ...
The contribution of the Trp/Met/Phe residues to physical interactions
... and Met243 Currently, there is no indication for the contribution of Trp146 for the p53 protein–protein interaction. Phe212 contributes to the p53–p53 core domain dimeric interactions observed in the p53–DNA complex [49]. It is important for the dynamic stability of the p53–p53 core domain dimmer [5 ...
... and Met243 Currently, there is no indication for the contribution of Trp146 for the p53 protein–protein interaction. Phe212 contributes to the p53–p53 core domain dimeric interactions observed in the p53–DNA complex [49]. It is important for the dynamic stability of the p53–p53 core domain dimmer [5 ...
Print
... of glycoprotein heterotrimers composed of an ␣, , and ␥ chain. To date, 5␣, 4, and 3␥ laminin chains have been identified that can combine to form 15 different isoforms. The laminin ␣-chains are considered to be the functionally important portion of the heterotrimers, as they exhibit tissue-specif ...
... of glycoprotein heterotrimers composed of an ␣, , and ␥ chain. To date, 5␣, 4, and 3␥ laminin chains have been identified that can combine to form 15 different isoforms. The laminin ␣-chains are considered to be the functionally important portion of the heterotrimers, as they exhibit tissue-specif ...
Characterization of the ATPase Activity of the Mr 170,000 to 180,000
... active efflux pump are not fully understood. Multidrug-resistant cells show characteristic changes in sur face glycoproteins. An overexpression of a plasma membrane glycoprotein of relative molecular mass (A/r 170,000-180,000) (P-glycoprotein) is consistently found in different multidrugresistant hu ...
... active efflux pump are not fully understood. Multidrug-resistant cells show characteristic changes in sur face glycoproteins. An overexpression of a plasma membrane glycoprotein of relative molecular mass (A/r 170,000-180,000) (P-glycoprotein) is consistently found in different multidrugresistant hu ...
Identification and Characterization of the Acid Phosphatase HppA in
... should be rapidly removed from the bacterial cell to avoid lethal toxicity. We therefore suspect that there is an underlying mechanism by which H. pylori effectively controls ammonium flux across the bacterial membrane, perhaps in conjunction with metal cation transport, which is thought to be essen ...
... should be rapidly removed from the bacterial cell to avoid lethal toxicity. We therefore suspect that there is an underlying mechanism by which H. pylori effectively controls ammonium flux across the bacterial membrane, perhaps in conjunction with metal cation transport, which is thought to be essen ...
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... Nakatani et al., 1999), but the notochord cells appear rounded and fail to adopt the wild-type 1⫻40 ‘stack of coins’ morphology. Markers for other embryonic tissues, including tail muscle, nervous system and epidermis, are correctly expressed within the context of a short and aberrant tail (Deschet ...
... Nakatani et al., 1999), but the notochord cells appear rounded and fail to adopt the wild-type 1⫻40 ‘stack of coins’ morphology. Markers for other embryonic tissues, including tail muscle, nervous system and epidermis, are correctly expressed within the context of a short and aberrant tail (Deschet ...
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... mechanisms similar to those used by vertebrates (Jiang et al., 2005; Munro and Odell, 2002b), and require an intact extracellular matrix (Veeman et al., 2008). Formation of intercellular lumens similar to vacuoles also contributes to notochord elongation (Dong et al., 2009; Miyamoto and Crowther, 19 ...
... mechanisms similar to those used by vertebrates (Jiang et al., 2005; Munro and Odell, 2002b), and require an intact extracellular matrix (Veeman et al., 2008). Formation of intercellular lumens similar to vacuoles also contributes to notochord elongation (Dong et al., 2009; Miyamoto and Crowther, 19 ...
The Pathogen-Actin Connection: A Platform for Defense Signaling in
... protein localization, activation, and signaling, presumably, the function of which is to activate defense and resistance signaling (17, 93, 115). Thus, it is not surprising that the host cell has evolved a suite of sophisticated mechanisms to assist in the reallocation of resources to combat pathoge ...
... protein localization, activation, and signaling, presumably, the function of which is to activate defense and resistance signaling (17, 93, 115). Thus, it is not surprising that the host cell has evolved a suite of sophisticated mechanisms to assist in the reallocation of resources to combat pathoge ...
Evolutionarily conserved recognition and innate immunity to fungal
... To identify mammalian receptors involved in the host response to fungal pathogens, and to test if the mammalian orthologues of the nematode scavenger receptors CED-1 and C03F11.3 are also involved in the host response to C. neoformans infection, we used a mouse shRNA library targeting innate immune ...
... To identify mammalian receptors involved in the host response to fungal pathogens, and to test if the mammalian orthologues of the nematode scavenger receptors CED-1 and C03F11.3 are also involved in the host response to C. neoformans infection, we used a mouse shRNA library targeting innate immune ...
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... squamous and was accompanied by heavy molting. The results obtained with hydrocortisone treatment were comparable to those in which hormones were omitted from the medium. Growth hormone. Explants cultured with growth hormone showed a small amount of cartilage differentiation. The regeneration area w ...
... squamous and was accompanied by heavy molting. The results obtained with hydrocortisone treatment were comparable to those in which hormones were omitted from the medium. Growth hormone. Explants cultured with growth hormone showed a small amount of cartilage differentiation. The regeneration area w ...
Synthesis and Localization of Ciliary Neurotrophic Factor in the
... 1991): 26 picograms of a shortened synthetic CNTF-RNA standard covering the whole coding sequence of the CNTF mRNA (600 bp) were added to each tissue sample as a recovery standard before RNA extraction. After electrophoresis through a 1.4% agarose gel containing 2.0 M formaldehyde (Lehrach et al., 1 ...
... 1991): 26 picograms of a shortened synthetic CNTF-RNA standard covering the whole coding sequence of the CNTF mRNA (600 bp) were added to each tissue sample as a recovery standard before RNA extraction. After electrophoresis through a 1.4% agarose gel containing 2.0 M formaldehyde (Lehrach et al., 1 ...
... III or PDE IV results in a partial attenuation of total cAMP-hydrolysing capacity. Only if both isoenzymes are simultaneously inhibited will intracellular cAMP concentrations and PKA activity be sufficiently elevated for a complete inhibition of TNF-α release. Similar effects of PDE inhibitors on T- ...
Soluble factors from human endometrium promote angiogenesis
... maintained both EC (for which it was designed) and endometrium, since the appearance of disaggregated endometrium after 4 days in this culture medium was equivalent to that of endometrium cultured in DMEM/Hams F12 (50:50 mix) supplemented with 10% FCS (data not shown). No reproductive steroids were ...
... maintained both EC (for which it was designed) and endometrium, since the appearance of disaggregated endometrium after 4 days in this culture medium was equivalent to that of endometrium cultured in DMEM/Hams F12 (50:50 mix) supplemented with 10% FCS (data not shown). No reproductive steroids were ...
Western et al., 2001 - UBC Blogs
... Figure 3. Structure and development of wild-type and mutant seed coats. Plastic sections of tissue fixed in 3% (v/v) aqueous glutaraldehyde (unless otherwise stated) and stained with Toluidine blue. A, Mature wild-type ovule (0 d after pollination [DAP]). B, Wild-type seed coat at 4 DAP. Amyloplasts ...
... Figure 3. Structure and development of wild-type and mutant seed coats. Plastic sections of tissue fixed in 3% (v/v) aqueous glutaraldehyde (unless otherwise stated) and stained with Toluidine blue. A, Mature wild-type ovule (0 d after pollination [DAP]). B, Wild-type seed coat at 4 DAP. Amyloplasts ...
Perspectives
... months in my own lab were spent doing just that—reading and thinking. Then, during a long weekend of 14-hr days, it all poured out in the text for two full grant proposals that described the areas in which I would work for the next decade and beyond. This was a time when the cell cycle was being wor ...
... months in my own lab were spent doing just that—reading and thinking. Then, during a long weekend of 14-hr days, it all poured out in the text for two full grant proposals that described the areas in which I would work for the next decade and beyond. This was a time when the cell cycle was being wor ...
β-catenin controls differentiation of the retinal pigment epithelium in
... in scattered cells at embryonic and postnatal ages (Fig. 1B,C); however, activation ceases after postnatal day 30 (P30; not shown). RPE-specific reporter activation was confirmed up to P16 by colocalization of β-galactosidase and Otx2 expression (Fig. 1D-F; see also Fig. S1 in the supplementary mate ...
... in scattered cells at embryonic and postnatal ages (Fig. 1B,C); however, activation ceases after postnatal day 30 (P30; not shown). RPE-specific reporter activation was confirmed up to P16 by colocalization of β-galactosidase and Otx2 expression (Fig. 1D-F; see also Fig. S1 in the supplementary mate ...