1 PLASTID OSMOTIC STRESS INFLUENCES CELL
... This phenotype was not altered by growth on 2 µM NAA (Fig. 3B), indicating that NAA treatment ...
... This phenotype was not altered by growth on 2 µM NAA (Fig. 3B), indicating that NAA treatment ...
ABSTRACT Title of Document:
... progenitors enter the thymus in three waves during embryonic life (e6.5, e12 and e18). T cell progenitors from each wave undergo a period of extensive proliferation within the thymic cortex that lasts up to three weeks (e7 - day7 posthatch, e12 - d12, and e18- d18) (Cooper et al., 1991). These waves ...
... progenitors enter the thymus in three waves during embryonic life (e6.5, e12 and e18). T cell progenitors from each wave undergo a period of extensive proliferation within the thymic cortex that lasts up to three weeks (e7 - day7 posthatch, e12 - d12, and e18- d18) (Cooper et al., 1991). These waves ...
Cell lysate and SR-enriched membrane fraction (SR - HAL
... During the past decade, research has focused on the actions of aldosterone in target organs beyond the kidney, expanding the role of the aldosterone pathway in cardiovascular pathogenesis 1. Indeed, mineralocorticoid receptors (MR), which mainly underlie aldosterone action, have been detected in a r ...
... During the past decade, research has focused on the actions of aldosterone in target organs beyond the kidney, expanding the role of the aldosterone pathway in cardiovascular pathogenesis 1. Indeed, mineralocorticoid receptors (MR), which mainly underlie aldosterone action, have been detected in a r ...
Functional Characterization of the spf/ash Splicing Variation in OTC
... natural 5’ splice site (5’ss) or the cryptic c.386+4 splice site, specific forward primers were designed hybridizing with exon 4 or exon 4 plus the first four intronic nucleotides and exonic vector sequences, respectively (NAT: 5’-ACACCGCTCGACCTGGAGATC-3’ and CRYP: 5’CGCTCGGTTTACCTGGAGATC-3’). Both ...
... natural 5’ splice site (5’ss) or the cryptic c.386+4 splice site, specific forward primers were designed hybridizing with exon 4 or exon 4 plus the first four intronic nucleotides and exonic vector sequences, respectively (NAT: 5’-ACACCGCTCGACCTGGAGATC-3’ and CRYP: 5’CGCTCGGTTTACCTGGAGATC-3’). Both ...
TATA BINDING PROTEIN AND ASSOCIATED FACTORS ... TRANSCRIPTION: CHARACTERIZATION OF THE B-TFIID ACTIVITY
... disease states. This control is exerted at a number of steps along the path from DNAto protein, including the transcription, processing, transport and translation of RNA. Regulation of these steps involves the complicated interplay between nucleic acids and proteins. This thesis describes a biochemi ...
... disease states. This control is exerted at a number of steps along the path from DNAto protein, including the transcription, processing, transport and translation of RNA. Regulation of these steps involves the complicated interplay between nucleic acids and proteins. This thesis describes a biochemi ...
CHAPTER 2 Literature Review I Bone Homeostasis
... (NF)-κβ ligand), a protein expressed on the osteoblast cell membrane, binds to RANK (Receptor activator of NF-κβ) a receptor located on the osteoclast membrane.8 This cell-to-cell interaction initiates a signaling cascade downstream of RANK/RANKL. RANKL interacts with RANK to recruit tumor necrosis ...
... (NF)-κβ ligand), a protein expressed on the osteoblast cell membrane, binds to RANK (Receptor activator of NF-κβ) a receptor located on the osteoclast membrane.8 This cell-to-cell interaction initiates a signaling cascade downstream of RANK/RANKL. RANKL interacts with RANK to recruit tumor necrosis ...
Regulation of nuclear poly(A) addition controls the expression of
... inhibitory motifs in vivo and is in a position to affect the function of the cleavage/polyadenylation apparatus. For this, we examined the effect of anti-U1A antibodies on the native cleavage/polyadenylation complex in nuclear extracts formed on the m secretory poly(A) site in the presence and absen ...
... inhibitory motifs in vivo and is in a position to affect the function of the cleavage/polyadenylation apparatus. For this, we examined the effect of anti-U1A antibodies on the native cleavage/polyadenylation complex in nuclear extracts formed on the m secretory poly(A) site in the presence and absen ...
Close - Cold Spring Harbor Monograph Archive
... 0RF2. However, it is not ruled out that ORF1 or its product may have some additional role. The antizyme shift sequence, UCC-UGA-U, is unlike other known shift sites (Fig. 1, bottom). In vitro protein synthesis experiments in reticulocyte lysates established that serine (UCC) is the last amino acid e ...
... 0RF2. However, it is not ruled out that ORF1 or its product may have some additional role. The antizyme shift sequence, UCC-UGA-U, is unlike other known shift sites (Fig. 1, bottom). In vitro protein synthesis experiments in reticulocyte lysates established that serine (UCC) is the last amino acid e ...
From filaments to function: The role of the plant actin cytoskeleton in
... trafficking, while actin-dependent, functions independently of microtubules. In a parallel study, Ribeiro et al. (2013) came to a similar conclusion, demonstrating that nucleoprotein trafficking is actin-dependent and microtubule-independent, while further showing that actin was not required for the a ...
... trafficking, while actin-dependent, functions independently of microtubules. In a parallel study, Ribeiro et al. (2013) came to a similar conclusion, demonstrating that nucleoprotein trafficking is actin-dependent and microtubule-independent, while further showing that actin was not required for the a ...
Seed Germination and Dormancy
... (Lane, 1991), which is an oxalate oxidase. However, by its nature, the timing of its synthesis, and its potential metabolic functions (Lane, 1994), germin is more likely to be involved with postgerminative cell elongation. Thus, the search for specific protein markers with an exclusive role in germi ...
... (Lane, 1991), which is an oxalate oxidase. However, by its nature, the timing of its synthesis, and its potential metabolic functions (Lane, 1994), germin is more likely to be involved with postgerminative cell elongation. Thus, the search for specific protein markers with an exclusive role in germi ...
Paired and LIM class homeodomain proteins coordinate
... LET-23/EGFR in ALA. In wild type, a let-23:LET-23-GFP translational reporter is expressed in several non-neuronal cells and a small number of neurons, including ALA (Van Buskirk and Sternberg, 2007). We examined the expression of this reporter in ceh-17(np1) null mutant animals and found its express ...
... LET-23/EGFR in ALA. In wild type, a let-23:LET-23-GFP translational reporter is expressed in several non-neuronal cells and a small number of neurons, including ALA (Van Buskirk and Sternberg, 2007). We examined the expression of this reporter in ceh-17(np1) null mutant animals and found its express ...
PDF
... et al., 2011). Moreover, interfering with the actin cytoskeleton during mitosis results in increased pulling forces on the anterior side (Afshar et al., 2010; Berends et al., 2013), although whether this occurs via an impact on ternary complex components, and whether a similar relationship pertains ...
... et al., 2011). Moreover, interfering with the actin cytoskeleton during mitosis results in increased pulling forces on the anterior side (Afshar et al., 2010; Berends et al., 2013), although whether this occurs via an impact on ternary complex components, and whether a similar relationship pertains ...
How mammals sense infection: from endotoxin to the Toll-like receptors Bruce Beutler
... • Preparative native gel electrophoresis • Preparative SDS gel electrophoresis • Yielded microgram quantities of an apparently pure 17.5 kD protein with approximately 2% yield of initial biological activity (prior to denaturing gel electrophoresis). • Cachectin comprised 1-2% of the protein secreted ...
... • Preparative native gel electrophoresis • Preparative SDS gel electrophoresis • Yielded microgram quantities of an apparently pure 17.5 kD protein with approximately 2% yield of initial biological activity (prior to denaturing gel electrophoresis). • Cachectin comprised 1-2% of the protein secreted ...
Effect of ABA signalling in primary cell wall Minor Thesis Report
... Numerous studies of molecular, biochemical and genetic research describe three main components involves in ABA signalling pathway; type 2C protein phosphatase (PP2C), SNF1 related protein kinase (SnRKs) and soluble ABA receptors Pyrabactin resistant (PYR/PYL) and regulatory components of ABA recepto ...
... Numerous studies of molecular, biochemical and genetic research describe three main components involves in ABA signalling pathway; type 2C protein phosphatase (PP2C), SNF1 related protein kinase (SnRKs) and soluble ABA receptors Pyrabactin resistant (PYR/PYL) and regulatory components of ABA recepto ...
Ways of Ion Channel Gating in Plant Cells
... channels which become active at depolarized membrane potentials, but their respective activation depends on the cytoplasmic Ca2 level (De Boer and Wegner, 1997). KORC, NORC and SKOR are dierent channels from plasmalemma of root xylem parenchyma cells. They are responsible for xylem loading (Robert ...
... channels which become active at depolarized membrane potentials, but their respective activation depends on the cytoplasmic Ca2 level (De Boer and Wegner, 1997). KORC, NORC and SKOR are dierent channels from plasmalemma of root xylem parenchyma cells. They are responsible for xylem loading (Robert ...
AtAMT1 gene expression and NH uptake in roots of glutamine levels
... cerevisiae (Ninneman et al., 1994). The predicted sequence of the AtAmt1 transporter was homologous with the Mep1 NH4+ transport protein from S. cerevisiae. Furthermore, when expressed heterologously in the mep1-1 mep2-1 double mutant, it functioned as a saturable high-af®nity transporter capable of ...
... cerevisiae (Ninneman et al., 1994). The predicted sequence of the AtAmt1 transporter was homologous with the Mep1 NH4+ transport protein from S. cerevisiae. Furthermore, when expressed heterologously in the mep1-1 mep2-1 double mutant, it functioned as a saturable high-af®nity transporter capable of ...
Lens Major Intrinsic Protein (MIP)
... adhesion and collapses the intercellular space due to its channel-forming properties (Zampighi et al., 1989). This would allow the formation of the 11-13-nm junctions with their extremely small interveningspaces. Since hydrophobicity plots predict that MIP leaves three small extracellular loops with ...
... adhesion and collapses the intercellular space due to its channel-forming properties (Zampighi et al., 1989). This would allow the formation of the 11-13-nm junctions with their extremely small interveningspaces. Since hydrophobicity plots predict that MIP leaves three small extracellular loops with ...
Antibody purification handbook
... polypeptide chains: two identical heavy (H) chains, each carrying covalently attached oligosaccharide groups; and two identical, non-glycosylated light (L) chains. A disulphide bond joins a heavy chain and a light chain together. The heavy chains are also joined to each other by disulphide bonds. Th ...
... polypeptide chains: two identical heavy (H) chains, each carrying covalently attached oligosaccharide groups; and two identical, non-glycosylated light (L) chains. A disulphide bond joins a heavy chain and a light chain together. The heavy chains are also joined to each other by disulphide bonds. Th ...
Bacterial Quorum-Sensing Network Architectures
... and a cognate cytoplasmic response regulator, which functions as a transcriptional regulator (39, 41, 109). A general model for oligopeptidemediated quorum sensing is depicted in Figure 3. As in AHL quorum-sensing systems, the concentration of secreted oligopeptide autoinducer increases as the cell ...
... and a cognate cytoplasmic response regulator, which functions as a transcriptional regulator (39, 41, 109). A general model for oligopeptidemediated quorum sensing is depicted in Figure 3. As in AHL quorum-sensing systems, the concentration of secreted oligopeptide autoinducer increases as the cell ...
Splice-switching efficiency and specificity for oligonucleotides with
... LNA monomers, where every second position was an LNA monomer. One analogue was a phosphorothioate LNA/2 -OMe RNA mixmer (LNA2-2OMe) designed to investigate splice-switching efficiency when increasing the proportion of LNA monomers from 33 % to 50 % (Table 1). The other analogue was a phosphorothioa ...
... LNA monomers, where every second position was an LNA monomer. One analogue was a phosphorothioate LNA/2 -OMe RNA mixmer (LNA2-2OMe) designed to investigate splice-switching efficiency when increasing the proportion of LNA monomers from 33 % to 50 % (Table 1). The other analogue was a phosphorothioa ...
An evolutionarily conserved mechanism for cAMP elicited axonal
... nerve cord; (all but two of these neurons are motoneurons). Quantification (described in methods) was carried out for 10 animals per genotype. (B) Endogenous Wnd protein levels are increased in PKACA expressing neurons. Ventral nerve cords were dissected from third instar larvae (BG380-Gal4 [WT cont ...
... nerve cord; (all but two of these neurons are motoneurons). Quantification (described in methods) was carried out for 10 animals per genotype. (B) Endogenous Wnd protein levels are increased in PKACA expressing neurons. Ventral nerve cords were dissected from third instar larvae (BG380-Gal4 [WT cont ...
PDF Version - Weizmann Institute of Science
... lane 2). These results and our additional restriction analysis (not shown) revealed that Cre deletes all elements of the concatenate, save the most 39 copy, which is truncated and whose remnant form is detected as a 5.6 kb single copy Eco RI fragment, as is shown schematically in Fig. 1C. Recombinas ...
... lane 2). These results and our additional restriction analysis (not shown) revealed that Cre deletes all elements of the concatenate, save the most 39 copy, which is truncated and whose remnant form is detected as a 5.6 kb single copy Eco RI fragment, as is shown schematically in Fig. 1C. Recombinas ...
PDF hosted at the Radboud Repository of the Radboud University Nijmegen
... (RIG-I) and melanoma differentiation-associated gene 5 (Mda5) respond to virus infection by recognition of distinct RNA structures. Via the adaptor molecule mitochondrial antiviral signaling protein (MAVS), they start a signaling cascade that results in activation of interferon regulatory factors (I ...
... (RIG-I) and melanoma differentiation-associated gene 5 (Mda5) respond to virus infection by recognition of distinct RNA structures. Via the adaptor molecule mitochondrial antiviral signaling protein (MAVS), they start a signaling cascade that results in activation of interferon regulatory factors (I ...
GENETIC CONTROL OF TISSUE SPECIFIC GROWTH IN THE
... In most organisms, different tissues and organs grow at different rates relative to each other, suggesting underlying growth mechanisms that act tissue specifically. The mechanisms of tissue specific growth are less well understood than those governing the growth of an entire organism. To gain a bet ...
... In most organisms, different tissues and organs grow at different rates relative to each other, suggesting underlying growth mechanisms that act tissue specifically. The mechanisms of tissue specific growth are less well understood than those governing the growth of an entire organism. To gain a bet ...
Auxin and other signals on the move in plants Auxins are a class of
... expansion. Depending on the specific tissue, auxin may promote axial elongation (as in shoots), lateral expansion (as in root swelling), or isodiametric expansion (as in fruit growth). In some cases (coleoptile growth) auxin-promoted cellular expansion occurs in the absence of cell division. In othe ...
... expansion. Depending on the specific tissue, auxin may promote axial elongation (as in shoots), lateral expansion (as in root swelling), or isodiametric expansion (as in fruit growth). In some cases (coleoptile growth) auxin-promoted cellular expansion occurs in the absence of cell division. In othe ...