Biosynthesis of Peptidoglycan in Wall plus Membrane Preparations
... Incorporation of labelled nucleotide into walls The newly synthesized peptidoglycan cross-linked to existing wall was purified by extensive washing with TMM buffer containing I % SDS. Regardless of whether the UDPMurNAc-pentapeptide or UDP-N-acetylglucosamine was radioactive, approximately 2000 pmol ...
... Incorporation of labelled nucleotide into walls The newly synthesized peptidoglycan cross-linked to existing wall was purified by extensive washing with TMM buffer containing I % SDS. Regardless of whether the UDPMurNAc-pentapeptide or UDP-N-acetylglucosamine was radioactive, approximately 2000 pmol ...
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in
... light or gravity are mediated by asymmetric distribution of the phytohormone auxin1 – 3. Physiological studies implicated a specific transport system that relocates auxin laterally, thereby effecting differential growth4; however, neither the molecular components of this system nor the cellular mech ...
... light or gravity are mediated by asymmetric distribution of the phytohormone auxin1 – 3. Physiological studies implicated a specific transport system that relocates auxin laterally, thereby effecting differential growth4; however, neither the molecular components of this system nor the cellular mech ...
Biofuel cells and their development - ePrints Soton
... offer cost advantages over metallic catalysts, although this is not likely to be the case until the consumption of the enzyme is sufficient to merit large scale production. Widespread usage of a biocatalyst would however tend to lower the cost of producing it, while this is not true of transition me ...
... offer cost advantages over metallic catalysts, although this is not likely to be the case until the consumption of the enzyme is sufficient to merit large scale production. Widespread usage of a biocatalyst would however tend to lower the cost of producing it, while this is not true of transition me ...
as PDF
... oligomerization and conformational changes in AβP are important for its neurodegenerative capacity. There is considerable interest regarding the mechanism by which AβPs cause neurodegeneration. AβPs reportedly cause numerous adverse effects on neuronal survival, e.g., reactive oxygen species (ROS) p ...
... oligomerization and conformational changes in AβP are important for its neurodegenerative capacity. There is considerable interest regarding the mechanism by which AβPs cause neurodegeneration. AβPs reportedly cause numerous adverse effects on neuronal survival, e.g., reactive oxygen species (ROS) p ...
Organization and translation of mRNA in sympathetic axons
... uninterrupted connection to the neuronal cell body (Cajal, 1991), which provides a continuous supply of proteins and organelles generated there (Grafstein and Forman, 1980; Vallee and Bloom, 1991). However, axons do carry out autonomous synthesis of many macromolecules, notably lipids (Vance et al., ...
... uninterrupted connection to the neuronal cell body (Cajal, 1991), which provides a continuous supply of proteins and organelles generated there (Grafstein and Forman, 1980; Vallee and Bloom, 1991). However, axons do carry out autonomous synthesis of many macromolecules, notably lipids (Vance et al., ...
Actin Filaments Play a Critical Role in Vacuolar
... overexpression of wild-type actin2 does not affect morphology of actin filaments. By contrast, overexpression of actin2[D13K] or actin2[D13Q] in the protoplasts resulted in a diffuse staining pattern of GFP:talin (Figure 3A, panels c and e), which strongly suggests that transiently expressed actin2[ ...
... overexpression of wild-type actin2 does not affect morphology of actin filaments. By contrast, overexpression of actin2[D13K] or actin2[D13Q] in the protoplasts resulted in a diffuse staining pattern of GFP:talin (Figure 3A, panels c and e), which strongly suggests that transiently expressed actin2[ ...
Developmental Changes in the Subcellular Localization of Calretinin
... the plasma membrane in 86% of the cells examined (Fig. 2 A). This submembrane distribution was even more pronounced at E18 when the majority of the cells (91%) contained high levels of immunofluorescence in the form of a distinctive shell under the plasmamembrane. At posthatching day 2, the animals ...
... the plasma membrane in 86% of the cells examined (Fig. 2 A). This submembrane distribution was even more pronounced at E18 when the majority of the cells (91%) contained high levels of immunofluorescence in the form of a distinctive shell under the plasmamembrane. At posthatching day 2, the animals ...
NIH Public Access
... The decision for progenitors to differentiate is made at the G1 phase of the cell cycle where Dtype cyclins and cyclin-dependent kinases inhibitors (CKIs) play an important role in regulating cell cycle exit. The CKIs regulate cell cycle progression by blocking phosphorylation of the retinoblastoma ...
... The decision for progenitors to differentiate is made at the G1 phase of the cell cycle where Dtype cyclins and cyclin-dependent kinases inhibitors (CKIs) play an important role in regulating cell cycle exit. The CKIs regulate cell cycle progression by blocking phosphorylation of the retinoblastoma ...
Formation of Helical Hairpins during Membrane Protein Integration
... the uniformly hydrophobic segments was maximal for three added lysine residues, we compared helical hairpin formation in the absence and presence of three C-terminally ¯anking Lys residues for an additional series of constructs with the general composition K4L21XL7VL10K3P (where X is any of the 20 n ...
... the uniformly hydrophobic segments was maximal for three added lysine residues, we compared helical hairpin formation in the absence and presence of three C-terminally ¯anking Lys residues for an additional series of constructs with the general composition K4L21XL7VL10K3P (where X is any of the 20 n ...
Vertebral Column
... fibrosus (attaches to cartilaginous end plate) Connects vertebral bodies in a fibrocartilaginous joint (no capsule, little motion) ...
... fibrosus (attaches to cartilaginous end plate) Connects vertebral bodies in a fibrocartilaginous joint (no capsule, little motion) ...
Cholesterol and nicotinic acetylcholine receptor
... Michel Eugène Chevreul [34], although other authors (e.g. [101]) attribute the discovery, also in gallstones, to the French chemist and physician François Poulletier de la Salle, in 1789. It was only in 1859 that cholesterol was identified as an alcohol by Berthelot [59] and in 1888 F. Reinitzer det ...
... Michel Eugène Chevreul [34], although other authors (e.g. [101]) attribute the discovery, also in gallstones, to the French chemist and physician François Poulletier de la Salle, in 1789. It was only in 1859 that cholesterol was identified as an alcohol by Berthelot [59] and in 1888 F. Reinitzer det ...
Infusion of a biotinylated bis-glucose photolabel
... Fig. 2. Efficacy of heart bio-LC-ATB-BGPA GLUT4 photolabeling. A: regional homogeneity. Left ventricular (LV), right ventricular (RV), and intraventricular septum myocardial tissues were processed separately after photolabeling hearts perfused without insulin. Streptavidin-isolated photolabeled GLUT ...
... Fig. 2. Efficacy of heart bio-LC-ATB-BGPA GLUT4 photolabeling. A: regional homogeneity. Left ventricular (LV), right ventricular (RV), and intraventricular septum myocardial tissues were processed separately after photolabeling hearts perfused without insulin. Streptavidin-isolated photolabeled GLUT ...
Development
... 4888 H. R. M. Schlaman and others embryogenesis can be rescued either by the addition of rhizobial LCOs or by an endochitinase purified from wild-type embryos (de Jong et al., 1993). (3) Tobacco plants transgenic for Rhizobium nodA and/or nodB, which are involved in LCO production, are severely aff ...
... 4888 H. R. M. Schlaman and others embryogenesis can be rescued either by the addition of rhizobial LCOs or by an endochitinase purified from wild-type embryos (de Jong et al., 1993). (3) Tobacco plants transgenic for Rhizobium nodA and/or nodB, which are involved in LCO production, are severely aff ...
SM-20, EGL-9, and the EGLN Family of Hypoxia
... the plasma membrane to the nucleus has been shown to involve other types of protein modifications including regulated proteolysis (Ebinu and Yankner, 2002), fatty acid acylation (Resh, 1996), ubiquitination (Yang and Yu, 2003), and acetylation (Magnaghi-Jaulin et al., 1999). Recent studies on the re ...
... the plasma membrane to the nucleus has been shown to involve other types of protein modifications including regulated proteolysis (Ebinu and Yankner, 2002), fatty acid acylation (Resh, 1996), ubiquitination (Yang and Yu, 2003), and acetylation (Magnaghi-Jaulin et al., 1999). Recent studies on the re ...
Dynamics of small genetic circuits subject to stochastic partitioning
... large macromolecules in low copy number are independently segregated into the daughter cells. These macromolecules (denoted by B in Fig. 1B) reduce the volume accessible to other molecules, and the error in their partitioning is imparted to the segregated molecules (right side of Fig. 1B). If the sa ...
... large macromolecules in low copy number are independently segregated into the daughter cells. These macromolecules (denoted by B in Fig. 1B) reduce the volume accessible to other molecules, and the error in their partitioning is imparted to the segregated molecules (right side of Fig. 1B). If the sa ...
Sperm entry is sufficient to trigger division of the
... 2009). Although our cell fate study was limited by the number of sperm cell fate markers currently available, it suggested that cdka;1 did not affect sperm cell fate. However, we observed that the expression level of HTR10-RFP was variable in pollen produced by cdka;1/+ plants (Fig. 1F) and estimate ...
... 2009). Although our cell fate study was limited by the number of sperm cell fate markers currently available, it suggested that cdka;1 did not affect sperm cell fate. However, we observed that the expression level of HTR10-RFP was variable in pollen produced by cdka;1/+ plants (Fig. 1F) and estimate ...
OsRAN2, essential for mitosis, enhances cold tolerance in rice by
... compatible compound synthesis are involved in cold tolerance regulation in rice. These proteins include otsA and otsB, choline monooxygenase, and WFT1 and WFT2, which are responsible for the synthesis of trehalose, glycinebetaine and fructan, respectively (Garg et al. 2002; Shirasawa et al. 2006; Ka ...
... compatible compound synthesis are involved in cold tolerance regulation in rice. These proteins include otsA and otsB, choline monooxygenase, and WFT1 and WFT2, which are responsible for the synthesis of trehalose, glycinebetaine and fructan, respectively (Garg et al. 2002; Shirasawa et al. 2006; Ka ...
- Wiley Online Library
... well-known but little-studied phenomenon in living systems. Its presence in the mitochondria of NASH would offer significant insight into the disease with possible therapeutic implications. We postulated that intramitochondrial disturbances in NASH are sufficient to produce such transitions and that t ...
... well-known but little-studied phenomenon in living systems. Its presence in the mitochondria of NASH would offer significant insight into the disease with possible therapeutic implications. We postulated that intramitochondrial disturbances in NASH are sufficient to produce such transitions and that t ...
The Dual Function of Sugar Carriers: Transport and Sugar
... transporters were first cloned from Chlorella kessleri by exploiting their rapid induction following the addition of hexoses to the growth medium. Specifically, differential screening of cDNAs from autotrophic versus heterotrophic cells enabled the cloning of the first plant hexose transporter gene ...
... transporters were first cloned from Chlorella kessleri by exploiting their rapid induction following the addition of hexoses to the growth medium. Specifically, differential screening of cDNAs from autotrophic versus heterotrophic cells enabled the cloning of the first plant hexose transporter gene ...
Chapter 3 Cells and Tissues
... of substances is “up the concentration gradient”; this requires energy from ATP Ion pumps (Figure 3-5) • An ion pump is a protein complex in the cell membrane • Ion pumps use energy from ATP to move substances across cell membranes against their concentration gradients • Examples: sodium-potassium ...
... of substances is “up the concentration gradient”; this requires energy from ATP Ion pumps (Figure 3-5) • An ion pump is a protein complex in the cell membrane • Ion pumps use energy from ATP to move substances across cell membranes against their concentration gradients • Examples: sodium-potassium ...