The unique proline-rich domain of parotid proline
... pLEN expression vector (Neufeld et al., 1987). The signal and propeptide sequences of preproPTH with added 5′ SalI and 3′ HindIII sites were synthesized by PCR, inserted into pBluescript, and the primary structure was confirmed by DNA sequencing. tG cDNA lacking nucleotides encoding a signal sequenc ...
... pLEN expression vector (Neufeld et al., 1987). The signal and propeptide sequences of preproPTH with added 5′ SalI and 3′ HindIII sites were synthesized by PCR, inserted into pBluescript, and the primary structure was confirmed by DNA sequencing. tG cDNA lacking nucleotides encoding a signal sequenc ...
Cytokinesis of neuroepithelial cells can divide their basal process
... M-phase NE cell bodies but also their basal processes extending radially towards the basal side of the neuroepithelium. Arrowhead, a globular-shaped M-phase NE cell body. The asterisk indicates ectodermal cells, which were stained by the secondary anti-mouse antibody. V, ventricle; VZ, ventricular z ...
... M-phase NE cell bodies but also their basal processes extending radially towards the basal side of the neuroepithelium. Arrowhead, a globular-shaped M-phase NE cell body. The asterisk indicates ectodermal cells, which were stained by the secondary anti-mouse antibody. V, ventricle; VZ, ventricular z ...
Auxin Metabolism and Function in the Multicellular Brown Alga
... Ectocarpus siliculosus is a small brown alga that has recently been developed as a genetic model. Its thallus is filamentous, initially organized as a main primary filament composed of elongated cells and round cells, from which branches differentiate. Modeling of its early development suggests the ...
... Ectocarpus siliculosus is a small brown alga that has recently been developed as a genetic model. Its thallus is filamentous, initially organized as a main primary filament composed of elongated cells and round cells, from which branches differentiate. Modeling of its early development suggests the ...
The Differential Degradation of Two Cytosolic Proteins As a Tool to
... (1990), who followed sequential steps in autophagy after inducing artificial protein complexes in the ER. However, their observations were restricted to the degradation of these complexes while, in general, autophagy is thought to be a nonspecific process in which cytoplasmic proteins are degraded r ...
... (1990), who followed sequential steps in autophagy after inducing artificial protein complexes in the ER. However, their observations were restricted to the degradation of these complexes while, in general, autophagy is thought to be a nonspecific process in which cytoplasmic proteins are degraded r ...
Membrane Vesicles Isolated from Sugar Beet Leaves
... membrane level is quite limited. This is due not only to the difficulty encountered in the solubilization and reconstitution of membrane bound transport proteins, but also to the fact that the amino acid transport systems in plants are not well understood (20). For example, an amino acid transport p ...
... membrane level is quite limited. This is due not only to the difficulty encountered in the solubilization and reconstitution of membrane bound transport proteins, but also to the fact that the amino acid transport systems in plants are not well understood (20). For example, an amino acid transport p ...
Epstein-Barr virus-Associated Lymphomas in Malaysia: High
... BNLF1, which encodes for LMP-1, is not only essential for B-cell transformation ; it is also the only EBV gene that has transforming effects in non-lymphoid cells . It is considered to be a viral oncogene due to its ability to transform rodent fibroblasts in vitro and render them tumorigenic in nude ...
... BNLF1, which encodes for LMP-1, is not only essential for B-cell transformation ; it is also the only EBV gene that has transforming effects in non-lymphoid cells . It is considered to be a viral oncogene due to its ability to transform rodent fibroblasts in vitro and render them tumorigenic in nude ...
Specialization of oleosins in OB dynamics during seed development
... cell wall stains, no fluorescent dye allowed discriminating OBs from cell walls in living embryos (Figure 3-1 a, b). Consequently, individual plant cells could not be detected, and we used estimators of the distance between each OB to its neighbors to answer the question of distribution in the 3D sp ...
... cell wall stains, no fluorescent dye allowed discriminating OBs from cell walls in living embryos (Figure 3-1 a, b). Consequently, individual plant cells could not be detected, and we used estimators of the distance between each OB to its neighbors to answer the question of distribution in the 3D sp ...
Liquid-Tissue Mechanics in Amphibian Gastrulation: Germ
... gent to) a liquid interface which opposes pairs of fragments of gastrula and neurula any increase in its surface area, if cell subsurface germ layers, endoderm tends to aggregates behave like liquid droplets, surround ectoderm and mesoderm. Steinthen, in the absence of external deforming berg and Ke ...
... gent to) a liquid interface which opposes pairs of fragments of gastrula and neurula any increase in its surface area, if cell subsurface germ layers, endoderm tends to aggregates behave like liquid droplets, surround ectoderm and mesoderm. Steinthen, in the absence of external deforming berg and Ke ...
The Balance between Cell Division and Endoreplication Depends
... and by specific degradation through the ubiquitin pathway, involving the activity of the SCFSKP2A complex. In vivo data show that E2FC and DPB form a heterodimer. Overexpression of DPB and a stable form of E2FC (del Pozo et al., 2002) produces severe defects in cotyledon and leaf development. On the ...
... and by specific degradation through the ubiquitin pathway, involving the activity of the SCFSKP2A complex. In vivo data show that E2FC and DPB form a heterodimer. Overexpression of DPB and a stable form of E2FC (del Pozo et al., 2002) produces severe defects in cotyledon and leaf development. On the ...
"Redundancy " & "Junk" for Codons
... A third type of disease prion disease all centers around the tRNA bringing the correct amino acid along with its correct directions in terms of amount (expression) & longevity (dwell time)for the ribosome to make the protein possessing the correct structure/information-function [as] dictated by the ...
... A third type of disease prion disease all centers around the tRNA bringing the correct amino acid along with its correct directions in terms of amount (expression) & longevity (dwell time)for the ribosome to make the protein possessing the correct structure/information-function [as] dictated by the ...
Molecular Cloning of CD68, a Human Macrophage
... Tissue macrophages are involved in many immune functions, the most significantbeing phagocytosis of foreign and necrotic material, and antigen processing and presentation.2 The surface phenotype of mononuclear phagocytes is poorly defined by specific monoclonal antibodies (MoAbs), compared with othe ...
... Tissue macrophages are involved in many immune functions, the most significantbeing phagocytosis of foreign and necrotic material, and antigen processing and presentation.2 The surface phenotype of mononuclear phagocytes is poorly defined by specific monoclonal antibodies (MoAbs), compared with othe ...
THE DYNAMIN SUPERFAMILY: UNIVERSAL MEMBRANE
... which are involved in oligomerization and regulation of the GTPase activity (FIG. 2). The GTPase domain contains the GTP-binding motifs (G1–G4) that are needed for guanine-nucleotide binding and hydrolysis (FIG. 3). The conservation of these motifs is absolute except for the G4 motif in guanylate-bi ...
... which are involved in oligomerization and regulation of the GTPase activity (FIG. 2). The GTPase domain contains the GTP-binding motifs (G1–G4) that are needed for guanine-nucleotide binding and hydrolysis (FIG. 3). The conservation of these motifs is absolute except for the G4 motif in guanylate-bi ...
Cell cycle exit during terminal erythroid differentiation is associated
... probe per milliliter hybridization buffer. Blots were washed twice with 2 ⫻ SSC, 0.5% SDS at room temperature for 10 minutes each and twice with 0.1 ⫻ SSC, 0.1% SDS at 55°C for 40 minutes each. Full-length Mo-MLV DNA23 was used to probe for the detection of murine retroviral sequences. The mouse -g ...
... probe per milliliter hybridization buffer. Blots were washed twice with 2 ⫻ SSC, 0.5% SDS at room temperature for 10 minutes each and twice with 0.1 ⫻ SSC, 0.1% SDS at 55°C for 40 minutes each. Full-length Mo-MLV DNA23 was used to probe for the detection of murine retroviral sequences. The mouse -g ...
Lysosomes in iron metabolism, ageing and apoptosis
... It was the discoveries made by Christian de Duve and his coworkers in the late 1950s that brought lysosomes to wider notice. The group, based at the Catholic University in Lovain, Belgium, was at the time conducting studies of glucose-6-phosphatase (reviewed in de Duve 2005). As a reference enzyme, ...
... It was the discoveries made by Christian de Duve and his coworkers in the late 1950s that brought lysosomes to wider notice. The group, based at the Catholic University in Lovain, Belgium, was at the time conducting studies of glucose-6-phosphatase (reviewed in de Duve 2005). As a reference enzyme, ...
BE WHEN THEY GROW UP? Lessons from Epidermal Patterning in
... during differentiation, reaching average nuclear DNA levels of 20C–32C (34, 63), a process known as either endoreplication or endoreduplication (19). Endoreplication is a common variant of the cell cycle in which mitosis and cytokinesis are suppressed, but cycles of DNA replication continue. The rea ...
... during differentiation, reaching average nuclear DNA levels of 20C–32C (34, 63), a process known as either endoreplication or endoreduplication (19). Endoreplication is a common variant of the cell cycle in which mitosis and cytokinesis are suppressed, but cycles of DNA replication continue. The rea ...
Protists - Needham.K12.ma.us
... Protozoans with cilia are called ciliates. Cilia: Hair like projections from a cell that move with a wavelike motion. Just like pseudopods, ciliates use cilia to move and obtain food. Ciliates have complex cells. Many have more than one contractile vacuole and some (like paramecium), have 2 nuclei. ...
... Protozoans with cilia are called ciliates. Cilia: Hair like projections from a cell that move with a wavelike motion. Just like pseudopods, ciliates use cilia to move and obtain food. Ciliates have complex cells. Many have more than one contractile vacuole and some (like paramecium), have 2 nuclei. ...
Asymmetric cell division and axis formation in the embryo
... 2.3. PAR proteins and polarity establishment Contraction of the actomyosin network is crucial for establishing asymmetric distribution of PAR proteins in one-cell stage embryos (Figure 2, Figure 3). The six par genes (for partitioning defective) were identified in screens for maternal-effect mutatio ...
... 2.3. PAR proteins and polarity establishment Contraction of the actomyosin network is crucial for establishing asymmetric distribution of PAR proteins in one-cell stage embryos (Figure 2, Figure 3). The six par genes (for partitioning defective) were identified in screens for maternal-effect mutatio ...
Coupling cellular oscillators—circadian and cell division cycles in cyanobacteria Bernardo F Pando
... dark cycles. The question of how the cell division machinery is affected by this player was still open. Following up on this work, Mackey et al. [24] reported a screen based on the yeast two-hybrid assay, with which they identified the gene CdpA that interacts with CikA and has an effect on the elo ...
... dark cycles. The question of how the cell division machinery is affected by this player was still open. Following up on this work, Mackey et al. [24] reported a screen based on the yeast two-hybrid assay, with which they identified the gene CdpA that interacts with CikA and has an effect on the elo ...
Amyloplasts and Vacuolar Membrane Dynamics in
... isolated several shoot gravitropism (sgr) mutants, which are defective in the gravitropic response of inflorescence stems. In the Arabidopsis shoot, the epidermis, cortex, endodermis, and stele containing vascular tissues are arranged in a radially symmetrical manner. In the course of the study of s ...
... isolated several shoot gravitropism (sgr) mutants, which are defective in the gravitropic response of inflorescence stems. In the Arabidopsis shoot, the epidermis, cortex, endodermis, and stele containing vascular tissues are arranged in a radially symmetrical manner. In the course of the study of s ...
From transporter to transceptor
... plasma membrane creates a high-affinity, high-capacity uptake system for amino acids. Gap1 is a highly promiscuous carrier that transports a wide variety of amino acids, including not only the regular protein amino acids but also nonmetabolizable amino acids like D-amino acids [1, 40]. Because of it ...
... plasma membrane creates a high-affinity, high-capacity uptake system for amino acids. Gap1 is a highly promiscuous carrier that transports a wide variety of amino acids, including not only the regular protein amino acids but also nonmetabolizable amino acids like D-amino acids [1, 40]. Because of it ...