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Chemical Microscopy Applied to Biological Systems
Chemical Microscopy Applied to Biological Systems

... deeper penetration of the light into the sample and usually entails less damage from photobleaching (4). However, this technique stands out the most for its definition of excitation volume, which occurs only at regions where the photon density is high; this makes the technique less susceptible to o ...
Lecture Notes - Pitt Honors Human Physiology
Lecture Notes - Pitt Honors Human Physiology

... Discussion point: What is the practical significance of this adaptation? As we discussed earlier, skeletal muscle contracts most efficiently when the overlap between actin and myosin is maximal. If the muscle is stretched or compressed substantially, then the tension produced by contraction is redu ...
Relating the mechanics of the primary plant cell
Relating the mechanics of the primary plant cell

... the wall), and this process changes the mechanical properties of pectin as it enables cross-linking by positively charged calcium ions. Paradoxically, the de-esterification of pectin has been linked with both increased and decreased cell wall stiffness in vivo (Palin and Geitmann, 2012). In the poll ...
ER storage diseases: a role for ERGIC
ER storage diseases: a role for ERGIC

... doi:10.1242/jcs.02977 ...
RIBOSOME-INACTIVATING PROTEINS: A Plant Perspective
RIBOSOME-INACTIVATING PROTEINS: A Plant Perspective

... only by tRNATrp from mammalian and avian cells and not by other tRNAs or tRNATrp from yeast (20–23). Given the variety of assays and assay conditions used for RIP analysis, some differences observed in previous studies may be explained by cofactor requirements. It is not known, however, whether the ...
immunodetection of arabinogalactan proteins in different types of
immunodetection of arabinogalactan proteins in different types of

... Arabinogalactan proteins (AGPs) are a diverse class of highly glycosylated plant cell surface proteoglycans. The protein backbone, rich in hydroxyproline, serine and threonine, makes up only 1–10% of the mass of the whole molecule. The glycan components consist of β(1→3)-linked galactan backbones wi ...
Journal of Cell Science • Advance article Rop, the
Journal of Cell Science • Advance article Rop, the

... vesicle fusion influences cell shape during cytokinesis. ...
PHAGOLYSOSOME FORMATION IN NORMAL AND
PHAGOLYSOSOME FORMATION IN NORMAL AND

Cadherins constitute a superfamily of transmembrane - HAL
Cadherins constitute a superfamily of transmembrane - HAL

... cadherin interaction. It provides an explanation for the force measurements of the interaction between opposing cadherin layers, which have previously been interpreted as resulting from three different interdigitating interactions [23]. It is in accord with observations of native junctions. The fact ...
Natriuretic Factor
Natriuretic Factor

... perfusion pressure or ...
collagen - MBBS Students Club
collagen - MBBS Students Club

... Formation of Pro- α-chains:  Pre-pro α-chains- contain a special amino acid sequence at their N-terminal.  This sequence acts as a signal that the newly synthesized polypepetide is destined for function out side the cell. ...
Plant Gravitropism. Unraveling the Ups and
Plant Gravitropism. Unraveling the Ups and

... which was typically used in studies of root gravitropism, has been refined with laser ablation techniques. Laser ablation of the central columella cells of Arabidopsis, which had the most readily sedimentable amyloplasts, caused the strongest inhibitory effect on root bending (Blancaflor et al., 199 ...
COLLAGEN - Rihs.com.pk
COLLAGEN - Rihs.com.pk

... Formation of Pro- α-chains:  Pre-pro α-chains- contain a special amino acid sequence at their N-terminal.  This sequence acts as a signal that the newly synthesized polypepetide is destined for function out side the cell. ...
Message in a bottle: small signalling peptide
Message in a bottle: small signalling peptide

... The mature, functional, secreted small signalling peptide is often perceived by a membrane-associated receptor and regulates a signal transduction pathway, but some are not secreted (Butenko et al., 2009; Matsuzaki et al., 2010; Matsubayashi, 2011; Murphy et al., 2012). To date, however, a mere hand ...
The Cytoskeleton as a Regulator and Target of
The Cytoskeleton as a Regulator and Target of

... cytoplasmic aggregation will require identification and characterization of proteins involved in the signaling pathways that induce cytoskeletal rearrangement and of proteins responsible for bringing about cytoskeletal reorganization and function. It is likely that this phenomenon utilizes regulator ...
Bleaching Chemiclas for the Kraft Pulping Industry
Bleaching Chemiclas for the Kraft Pulping Industry

... The hypo system is used to remove chlorine from process streams before they are vented to atmosphere and is also used to safely dispose of chlorine during process upsets such as shut downs and power failures. THE ROLE OF THE LABORATORY The chemical plant is an area where chemical reactions and elec ...
Expression Sequences and Distribution of Two Primary Cell
Expression Sequences and Distribution of Two Primary Cell

... In comparative studies of CAMs, particularly N-CAM, which has multiple polypeptide forms (Murray et al., 1986b), it is essential to determine as accurately as possible the specificity of the reagents used. The distribution of N-CAM in the development of Xenopus was studied with a polyclonal rabbit a ...
Rac1 accumulates in the nucleus during the G2 phase of the cell
Rac1 accumulates in the nucleus during the G2 phase of the cell

... type, we studied the distribution of these proteins in multiple cell lines including MDCK, COS-1, and porcine aortic endothelial (PAE) cells (Fig. 1, B and C) and ECV304 human bladder carcinoma, HeLa, and NIH 3T3 (not depicted). GFP-Rac1 ranged from 5 ± 2% nuclear in MDCK cells to 30 ± 5% nuclear in ...
Light Control of Plasma Membrane Recruitment Using
Light Control of Plasma Membrane Recruitment Using

... exhibit bright fluorescence after 30 min of incubation with PCB (Levskaya et al., 2009). Thus, the ability of a Phy fusion construct to bind PCB can be tested directly, independently of the recruitment assay described in Section 6. Phy-Y276H can also be used to test the quality of purified PCB (Sect ...
Urine Reflection of Changes in Blood
Urine Reflection of Changes in Blood

Regulation of Vascular Development - Size
Regulation of Vascular Development - Size

... vascular tissues must be able to carry out their functions in expanding organs; this is particularly challenging in grasses and other groups of plants where leaves and stems grow from the base, and vascular continuity must be maintained across this growth zone. The primary vascular system also retai ...
amoeba notes 14
amoeba notes 14

... color highlighter to color around the outside of each of the pseudopods. When looking at amoeba under a microscope, an observer will note that no amoeba looks the same as any other because the cell membrane is very flexible and allows for the amoeba to change shape. Color the cell membrane light blu ...
Notochord vacuoles are lysosome-related organelles that function in
Notochord vacuoles are lysosome-related organelles that function in

... the embryo before bone formation. This axial support is crucial for locomotion in embryos that develop externally, such as fish and amphibians, to evade predation. The pressure exerted from the inflating notochord vacuoles on the constricting perinotochordal basement membrane gives this structure it ...
Notochord vacuoles are lysosome-related organelles that function in
Notochord vacuoles are lysosome-related organelles that function in

Epithelial Tissue
Epithelial Tissue

... IUG ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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