Probing for Binding Regions of the FtsZ Protein Surface through Site
... limits the rate of constriction and may contribute to the force (3; also see related discussions at the F1000Prime website [4, 5] and in reference 6). The FtsZ protein comprises a globular domain that is structurally homologous to tubulin. This globular domain can be divided further into two subdoma ...
... limits the rate of constriction and may contribute to the force (3; also see related discussions at the F1000Prime website [4, 5] and in reference 6). The FtsZ protein comprises a globular domain that is structurally homologous to tubulin. This globular domain can be divided further into two subdoma ...
Mechanisms associated with phagocytosis of
... water. Arcobacter butzleri is an emerging potential zoonotic pathogen that can be isolated from environmental water sources, where they can establish endosymbiotic relationships with amoebas. The aim of this study was to describe the implication of mannose-binding proteins and membraneassociated rec ...
... water. Arcobacter butzleri is an emerging potential zoonotic pathogen that can be isolated from environmental water sources, where they can establish endosymbiotic relationships with amoebas. The aim of this study was to describe the implication of mannose-binding proteins and membraneassociated rec ...
FtsZ - Cytoskeleton, Inc.
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
Chloroplast anchoring: its implications for the
... the acceleration of chloroplast motility was initiated by the formation of Pfr, whereas the subsequent deceleration was regulated by photosynthesis (Dong et al., 1995, 1996). With the deceleration of chloroplast motility, the configuration of actin filaments along the outer periclinal walls changed ...
... the acceleration of chloroplast motility was initiated by the formation of Pfr, whereas the subsequent deceleration was regulated by photosynthesis (Dong et al., 1995, 1996). With the deceleration of chloroplast motility, the configuration of actin filaments along the outer periclinal walls changed ...
Dictyostelium discoideum mutant synag 7 with altered G
... declines to an apparent equilibrium value approached at about 45-60 s. It has been demonstrated that this decrease in cyclic AMP binding appears to be due to an interconversion of high-affinity to low-affinity binding forms of the receptor (Van Haastert & De Wit, 1984c; Van Haastert, 1985; Van Haast ...
... declines to an apparent equilibrium value approached at about 45-60 s. It has been demonstrated that this decrease in cyclic AMP binding appears to be due to an interconversion of high-affinity to low-affinity binding forms of the receptor (Van Haastert & De Wit, 1984c; Van Haastert, 1985; Van Haast ...
Achilles` heel of Grapevine Downy Mildew
... friendly way. Not only are the metal ions highly toxic and persistent but also the synthetic fungicides require constant chemical adaptions because P. viticola rapidly evolves resistances. Therefore, breeding of resistant grapevine cultivars based on single resistance loci within the grapevine genom ...
... friendly way. Not only are the metal ions highly toxic and persistent but also the synthetic fungicides require constant chemical adaptions because P. viticola rapidly evolves resistances. Therefore, breeding of resistant grapevine cultivars based on single resistance loci within the grapevine genom ...
Plant cell walls to ethanol
... further cements the primary cell wall matrix. It accounts for 30–40 % of non-cellulosic polysaccharides in the primary cell walls of herbaceous dicotyledons and non-graminaceous monocots with significantly lesser amounts found in grasses, woody tissue and secondary cell walls [27,28]. Pectin consist ...
... further cements the primary cell wall matrix. It accounts for 30–40 % of non-cellulosic polysaccharides in the primary cell walls of herbaceous dicotyledons and non-graminaceous monocots with significantly lesser amounts found in grasses, woody tissue and secondary cell walls [27,28]. Pectin consist ...
A role for actin in regulating apoptosis/programmed cell death
... During apoptosis/PCD driven by the intrinsic pathway, the reception of an apoptotic stimulus leads to an increase in the permeability of the mitochondrial outer membrane, allowing proapoptotic molecules such as cytochrome c, AIF1 (apoptosisinducing factor 1) and Endo G (endonuclease G) to be release ...
... During apoptosis/PCD driven by the intrinsic pathway, the reception of an apoptotic stimulus leads to an increase in the permeability of the mitochondrial outer membrane, allowing proapoptotic molecules such as cytochrome c, AIF1 (apoptosisinducing factor 1) and Endo G (endonuclease G) to be release ...
A specific domain in α-catenin mediates binding to β
... complete binding the flow-through was reapplied to the settled beads. After washing the amylose beads 2× with 10 ml PBS, the fusion protein was eluted with 20 mM maltose in PBS, dialysed against 20 mM Tris-HCl, pH 8.0, and stored after shock freezing in liquid nitrogen. pMalα129 was constructed by d ...
... complete binding the flow-through was reapplied to the settled beads. After washing the amylose beads 2× with 10 ml PBS, the fusion protein was eluted with 20 mM maltose in PBS, dialysed against 20 mM Tris-HCl, pH 8.0, and stored after shock freezing in liquid nitrogen. pMalα129 was constructed by d ...
BCL-6 Negatively Regulates Expression of the NF- p50 Subunit B1 p105/
... nuclear extract of the BCL-6-positive Ly-1 cells. Fig. 2 shows that the sites located at ⫺1090 bp, ⫹2.7 kb, and ⫹20 kb formed specific BCL-6-DNA complexes that can be competed with an excess amount of the cold consensus BCL-6 oligo probes and supershifted by an anti-BCL-6 Ab. Furthermore, these thre ...
... nuclear extract of the BCL-6-positive Ly-1 cells. Fig. 2 shows that the sites located at ⫺1090 bp, ⫹2.7 kb, and ⫹20 kb formed specific BCL-6-DNA complexes that can be competed with an excess amount of the cold consensus BCL-6 oligo probes and supershifted by an anti-BCL-6 Ab. Furthermore, these thre ...
Unleashing the potential of the root hair cell as a
... Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA ...
... Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA ...
Role of hsp90 and the hsp90-binding immunophilins in signalling
... response for transactivation regardless of whether it is expressed in New World or Old World primate cells. They cloned the Guyanese squirrel monkey GR, and showed that it had the same high affinity binding activity as the human GR when expressed in COS-1 cells, but it had an order of magnitude high ...
... response for transactivation regardless of whether it is expressed in New World or Old World primate cells. They cloned the Guyanese squirrel monkey GR, and showed that it had the same high affinity binding activity as the human GR when expressed in COS-1 cells, but it had an order of magnitude high ...
Actin branching in the initiation and maintenance of lamellipodia
... of highly branched arrays of short filaments, 30-150nm long (Svitkina et al, 1997) (Svitkina & Borisy, 1999). The simultaneous observation that the Arp2/3 complex catalyzed the branching of actin filaments in vitro (Amann & Pollard, 2001) and localized specifically to lamellipodia (Svitkina & Borisy ...
... of highly branched arrays of short filaments, 30-150nm long (Svitkina et al, 1997) (Svitkina & Borisy, 1999). The simultaneous observation that the Arp2/3 complex catalyzed the branching of actin filaments in vitro (Amann & Pollard, 2001) and localized specifically to lamellipodia (Svitkina & Borisy ...
Outer Membrane Vesicle-Mediated Export of
... which has recently been referred to as the “Type 0” (zero) secretion system [14]. Outer membrane vesicles (OMVs) (diameter 20–200 nm) are constantly discharged from the surface of the Gram-negative bacteria during growth, and may entrap outer membrane proteins, LPS, phospholipids, and some periplas ...
... which has recently been referred to as the “Type 0” (zero) secretion system [14]. Outer membrane vesicles (OMVs) (diameter 20–200 nm) are constantly discharged from the surface of the Gram-negative bacteria during growth, and may entrap outer membrane proteins, LPS, phospholipids, and some periplas ...
Rapid Movement of Microtubules in Axons
... To visualize tubulin in axons, we injected rhodaminelabeled tubulin into the cell bodies of cultured rat sympathetic neurons 2–5 hr after plating and then observed the cells 2–24 hr later. To determine the extent of the incorporation of the rhodamine-labeled tubulin into microtubules, we visualized ...
... To visualize tubulin in axons, we injected rhodaminelabeled tubulin into the cell bodies of cultured rat sympathetic neurons 2–5 hr after plating and then observed the cells 2–24 hr later. To determine the extent of the incorporation of the rhodamine-labeled tubulin into microtubules, we visualized ...
S. cerevisiae Mitotic Exit Activator
... Cyclin-dependent kinases (CDKs) are the workhorses of the cell cycle. The CDK is the catalytic kinase subunit, which must associate with a regulatory cyclin subunit in order to be active (Morgan, 1997). The expression of each cyclin subunit is confined to a small window during the cell cycle via tra ...
... Cyclin-dependent kinases (CDKs) are the workhorses of the cell cycle. The CDK is the catalytic kinase subunit, which must associate with a regulatory cyclin subunit in order to be active (Morgan, 1997). The expression of each cyclin subunit is confined to a small window during the cell cycle via tra ...
The 14-3-3 gene par-5 is required for germline development and
... chromatin and smaller sizes (Fig. 4A). This effect, observed both in par-5(RNAi) and in par-5(it55) animals, was likely to be because of cells entering prematurely into mitosis before the DNA was properly replicated, thereby causing DNA fragmentation. To study this phenotype, we used an antibody aga ...
... chromatin and smaller sizes (Fig. 4A). This effect, observed both in par-5(RNAi) and in par-5(it55) animals, was likely to be because of cells entering prematurely into mitosis before the DNA was properly replicated, thereby causing DNA fragmentation. To study this phenotype, we used an antibody aga ...
View - Max-Planck
... (Figure 1). While most structural studies have focused on terrestrial plants, aquatic organisms account for around 50% of global carbon fixation (Field et al., 1998; Behrenfeld et al., 2001), and it is estimated that nearly half of this aquatic production is performed by eukaryotic algae (Jardillier ...
... (Figure 1). While most structural studies have focused on terrestrial plants, aquatic organisms account for around 50% of global carbon fixation (Field et al., 1998; Behrenfeld et al., 2001), and it is estimated that nearly half of this aquatic production is performed by eukaryotic algae (Jardillier ...
Monoclonal Antibody Characterization of Two Distant Sites
... major region ofthe protein is required for adhesive function (4, 38) . Studies comparing the biological activities of large and small fibronectin fusion fragments and synthetic peptides derived from fibronectin also indicate that most biological activity is lost when short fibronectin fragments and ...
... major region ofthe protein is required for adhesive function (4, 38) . Studies comparing the biological activities of large and small fibronectin fusion fragments and synthetic peptides derived from fibronectin also indicate that most biological activity is lost when short fibronectin fragments and ...
Regulation of the Function of the Human ABCG2 Multidrug
... microdomains, the so-called rafts, and its direct interaction with caveolin1, a marker of lipid rafts, has also been demonstrated (Storch et al., 2007). In accordance with this finding, high membrane cholesterol levels were found to significantly improve the function of ABCG2. We and others found th ...
... microdomains, the so-called rafts, and its direct interaction with caveolin1, a marker of lipid rafts, has also been demonstrated (Storch et al., 2007). In accordance with this finding, high membrane cholesterol levels were found to significantly improve the function of ABCG2. We and others found th ...