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Monoclonal Antibody Characterization of Two Distant Sites
Monoclonal Antibody Characterization of Two Distant Sites

... major region ofthe protein is required for adhesive function (4, 38) . Studies comparing the biological activities of large and small fibronectin fusion fragments and synthetic peptides derived from fibronectin also indicate that most biological activity is lost when short fibronectin fragments and ...
the signaling role of the accessory receptors cd2 and cd6 in t cell
the signaling role of the accessory receptors cd2 and cd6 in t cell

... identified and cloned, including isoforms lacking domain 1 or domain 3. Ensuing studies showed that the differential expression of the SRCR domain 3 resulted in a remarkable functional difference: whereas full-length CD6 targeted to the immunological synapse, CD6∆d3 was unable to localize at the T c ...
What is bad in cancer is good in the embryo: Importance of EMT in
What is bad in cancer is good in the embryo: Importance of EMT in

... Cadherin 6B during EMT: The loss of Cad6B is directly regulated by transcriptional repression of Snail2 prior to migration of NC in the chick [42]. This was thought to regulate the correct timing of NC emigration and delamination from the neural tube without affecting the expression of the migratory ...
The Plant Membrane-Associated REMORIN1.3 Accumulates in
The Plant Membrane-Associated REMORIN1.3 Accumulates in

... The online version of this article contains Web-only data. www.plantphysiol.org/cgi/doi/10.1104/pp.114.235804 ...
Nonsense-mediated mRNA decay - Case Western Reserve University
Nonsense-mediated mRNA decay - Case Western Reserve University

... an increased comprehension of the molecular interactions between the Upf proteins, translational termination and initiation factors, and the decay machinery is needed to reveal the actual molecular mechanism of NMD. In addition, there are several broader issues that still require consideration. For ...
VP5 autocleavage is required for efficient infection by in vitro
VP5 autocleavage is required for efficient infection by in vitro

... (4 kDa) and m1C (72 kDa), following putative autocleavage between residues Asn42 and Pro43 near the N terminus of the intact protein (Nibert et al., 1991, 2005). In addition, infection with intact virions, but not ISVPs, can be blocked by treating cells with a weak base, such as NH4Cl, that inhibits ...
Plasticity in Cell Division Patterns and Auxin
Plasticity in Cell Division Patterns and Auxin

... B. napus, neither the PIN1-GFP protein nor the PIN7-GFP protein was observed during male gametophyte development in planta. proDR5:GFP expression was observed during male gametophyte development in planta, but at an earlier stage than is used for microspore culture (Supplemental Figure 2D). In concl ...
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of

... Lys-48-linked polyubiquitin chains (1). The lysosome degrades extracellular and plasma membrane proteins brought there by endocytosis and cytoplasmic components delivered by autophagy. Various categories of autophagy have been defined differing in the delivery route of cytoplasmic material. These in ...
Extracellular matrix of the charophycean green algae
Extracellular matrix of the charophycean green algae

... flaccidum, and Spirogyra spp. exhibits significant biochemical diversity, ranging from distinct “land plant” polymers to polysaccharides unique to these algae. The neutral sugar composition of Chlorokybus atmophyticus hot water extract and Spirogyra extracellular polymeric substance (EPS), combined ...
The symbiotic ion channel homolog DMI1 is localized in the nuclear
The symbiotic ion channel homolog DMI1 is localized in the nuclear

... rhizobia may have co-opted part of the signaling pathway from the more ancient AM symbioses to evolve the younger nitrogen-fixing symbiosis in legumes. Many of these common symbiotic genes have recently been cloned and phylogenetic analyses have identified orthologs in non-legume species. The presen ...
Functional ultrastructure of the plant nucleolus
Functional ultrastructure of the plant nucleolus

... eukaryotic cells. They not only specialize in the production of ribosomal subunits but also play roles in many fundamental cellular activities. Concerning ribosome biosynthesis, particular stages of this process, i.e., ribosomal DNA transcription, primary RNA transcript processing, and ribosome asse ...
a cells- secrete glucagon
a cells- secrete glucagon

... Two important effects are: Insulin facilitates entry of glucose into muscle, adipose and several other tissues. The only mechanism by which cells can take up glucose is by facilitated diffusion through a family of glucose transporter In many tissues - muscle being a prime example - the major transpo ...
The Iron-Sulfur Cluster Proteins Isa1 and Isa2 Are Required for the
The Iron-Sulfur Cluster Proteins Isa1 and Isa2 Are Required for the

acetylcholine receptors in regenerating muscle
acetylcholine receptors in regenerating muscle

The versatility of mitochondrial calcium signals: From stimulation of
The versatility of mitochondrial calcium signals: From stimulation of

... reticulum Ca2+ ATPase; IP3R, IP3 receptor; mt, mitochondrion; ER, endoplasmic reticulum. In the insets the [Ca2+] measurements in different cellular compartments during agonist stimulation are represented. ...
The role of hyaluronic acid in protecting surface
The role of hyaluronic acid in protecting surface

... non-degraded HA on the lysing activity of PLA2 were examined in vitro. Liposomes (lipid model membrane) containing phosphatidylcholine (PC) were used as the substrate, on the assumption that they are appropriate representatives of SAPL. Results. HA adhered to the phospholipid membrane (liposomes), i ...
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PDF

... per gonad arm that can be detected at any given time is very low [4.0 apoptotic germ cells on average in hermaphrodites 48 hours post the fourth larval (L4) stage] (Gumienny et al., 1999). For this reason, we analyzed constitutive germ cell apoptosis in the background of ced-6(n2095). n2095 is a los ...
Acetylation of Polysaccharides in Plant Cell Wall
Acetylation of Polysaccharides in Plant Cell Wall

... Xylan is predominantly found in primary and secondary wall layers of grasses and in secondary wall layers of dicotyledons. It is made up of β, 1-4 linked Xyl units. Xylan from grasses is substituted with Ara, GlcA and methyl glucuronic acid (meGlcA) whereas in dicots secondary wall layers mostly Glc ...
Functional analysis of polar amino
Functional analysis of polar amino

Control of convergent yolk syncytial layer nuclear movement in
Control of convergent yolk syncytial layer nuclear movement in

... Fig. 1. Convergence movements are highly coordinated between iYSN and mesendoderm. (A,B) Bright-field images of an embryo at the beginning of gastrulation (6.5 hpf; A) and at mid-gastrulation (8.5 hpf; B). Animal pole is towards the top and dorsal is towards the right. Boxes delineate the imaged reg ...
Control of convergent yolk syncytial layer nuclear movement in
Control of convergent yolk syncytial layer nuclear movement in

Novel Mechanism of PTEN Regulation by Its
Novel Mechanism of PTEN Regulation by Its

Phloem Transport: Cellular Pathways and Molecular Trafficking
Phloem Transport: Cellular Pathways and Molecular Trafficking

as Hotspot Ca 2+ Signaling Units
as Hotspot Ca 2+ Signaling Units

... bidirectional relation between Ca2+ release and ATP production allows for a positive feedback regulation between ER and mitochondria during increased energetic demand [41]. The uptake of Ca2+ in mitochondria will also affect Ca2+ signaling at both the local and the global level. Assuming the microdo ...
Formation and excretion of autophagic plastids (plastolysomes) in
Formation and excretion of autophagic plastids (plastolysomes) in

... embryo-like structures. A qualitative (Figure 3) and quantitative (Table 1) study of plastids of these cells revealed that 60.7% of them presented conventional shapes, including round, elongated, bean-like, or sausage-like profiles (Figure 3A). In all of these plastids, the stroma appeared more elec ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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