Direct Interaction between Survivin and Smac/DIABLO Is Essential
... has been approved for the treatment of breast, ovarian, and lung cancers (25, 30 –32). We incubated HeLa cells with Taxol at a concentration of 100 nM, which is known to induce apoptosis in a number of different cell types. After 24-h incubation, the clear morphological changes characteristic of apo ...
... has been approved for the treatment of breast, ovarian, and lung cancers (25, 30 –32). We incubated HeLa cells with Taxol at a concentration of 100 nM, which is known to induce apoptosis in a number of different cell types. After 24-h incubation, the clear morphological changes characteristic of apo ...
Mitochondrial dysfunction in Parkinson`s disease
... evidence that ROS are not just the bad guys, but have physiological functions as signaling molecules in diverse cellular pathways (rev. in refs. [74–76]). Moreover, a mild increase in ROS formation may activate protective stress response pathways [77,78]. Hence, the common paradigm that mitochondria ...
... evidence that ROS are not just the bad guys, but have physiological functions as signaling molecules in diverse cellular pathways (rev. in refs. [74–76]). Moreover, a mild increase in ROS formation may activate protective stress response pathways [77,78]. Hence, the common paradigm that mitochondria ...
Decrease in Reactive Amino Groups during Oxidation or Endothelial
... in the extent of modification was encountered with different media supplemented with the same concentration of Cu + + In the present report we describe conditions for repro- ...
... in the extent of modification was encountered with different media supplemented with the same concentration of Cu + + In the present report we describe conditions for repro- ...
Zoology Lab Manual - Austin Community College
... 1. If you are sketching material without magnifcation or if you are switching back and forth between no magnification and slight magnification (eg. hand lens)you should draw a rectangular border around your sketch and indicate the actual size of the object being sketched. 2. if you are sketching mat ...
... 1. If you are sketching material without magnifcation or if you are switching back and forth between no magnification and slight magnification (eg. hand lens)you should draw a rectangular border around your sketch and indicate the actual size of the object being sketched. 2. if you are sketching mat ...
Neural induction in Xenopus requires early FGF signalling in
... 1E,F) in whole embryos. One caveat in these experiments lies in the fact that the degree of inhibition of the BMP pathway could be sufficient to suppress epidermis, but not to induce neural fates. We thus looked for additional indications that our conditions were indeed very effective at blocking BM ...
... 1E,F) in whole embryos. One caveat in these experiments lies in the fact that the degree of inhibition of the BMP pathway could be sufficient to suppress epidermis, but not to induce neural fates. We thus looked for additional indications that our conditions were indeed very effective at blocking BM ...
TEF30 interacts with photosystem II monomers and is involved in the
... together, our data suggest potential roles for TEF30 in facilitating the incorporation of a ...
... together, our data suggest potential roles for TEF30 in facilitating the incorporation of a ...
Lesions of and Surgical Approaches to the Petrous Apex
... Marrow space should follow same signal characteristics as orbital fat on MRI T1 and T2 O O ...
... Marrow space should follow same signal characteristics as orbital fat on MRI T1 and T2 O O ...
Ca signaling and early embryonic patterning during zebrafish
... generation of longer range, and even pan-embryonic, intercellular Ca2+ signals. However, by the end of the GP and during the subsequent Segmentation Period (SP), when the germ layers and major body axes are established, a more localized pattern of intercellular Ca2+ signaling then resumes. This is a ...
... generation of longer range, and even pan-embryonic, intercellular Ca2+ signals. However, by the end of the GP and during the subsequent Segmentation Period (SP), when the germ layers and major body axes are established, a more localized pattern of intercellular Ca2+ signaling then resumes. This is a ...
BACTERIAL FUNGAL INTERACTIONS IN THE CYSTIC FIBROSIS
... in a significant decrease in elastase produced by certain P. aeruginosa strains. ...
... in a significant decrease in elastase produced by certain P. aeruginosa strains. ...
Lignin biosynthesis in Norway spruce: from a model system to the tree
... genes within the gene families in all lignin-forming tissues. However, such coordinated expression was not found for peroxidase genes. We also studied stress-induced ...
... genes within the gene families in all lignin-forming tissues. However, such coordinated expression was not found for peroxidase genes. We also studied stress-induced ...
Melatonin Receptors Trigger cAMP Production and Inhibit Chloride
... Effect of Melatonin and 5-MCA-NAT on Ciliary Body Epithelial Cells. MQAE is a highly sensitive chloride fluorescence probe. This fluorescence is quenched in the presence of chloride so that changes in fluorescence are inversely proportional to changes in chloride concentration. By using the protocol ...
... Effect of Melatonin and 5-MCA-NAT on Ciliary Body Epithelial Cells. MQAE is a highly sensitive chloride fluorescence probe. This fluorescence is quenched in the presence of chloride so that changes in fluorescence are inversely proportional to changes in chloride concentration. By using the protocol ...
RenalTubularTransportof AminoAcids
... (89) provided a tool for more direct localization of renal tubular amino acid transport. These studies demonstrated that the naturally occurring amino acids were reabsorbed in a segment of the nephron coextensive with glucose reabsorption and p-aminohippurate secretion (90-96). Although an attempt t ...
... (89) provided a tool for more direct localization of renal tubular amino acid transport. These studies demonstrated that the naturally occurring amino acids were reabsorbed in a segment of the nephron coextensive with glucose reabsorption and p-aminohippurate secretion (90-96). Although an attempt t ...
- Gastroenterology
... pancreas. The first of these, trypsinogen, can be converted to active trypsin by the peptidase cathepsin B (CTSB). We investigated whether cathepsin L (CTSL) can also process trypsinogen to active trypsin and has a role in pancreatitis. METHODS: In CTSL-deficient (Ctsl⫺/⫺) mice, pancreatitis was ind ...
... pancreas. The first of these, trypsinogen, can be converted to active trypsin by the peptidase cathepsin B (CTSB). We investigated whether cathepsin L (CTSL) can also process trypsinogen to active trypsin and has a role in pancreatitis. METHODS: In CTSL-deficient (Ctsl⫺/⫺) mice, pancreatitis was ind ...
Youth Take Heart Teacher`s Manual
... THEIR OWN VALUES ARE. Heart Rate: The heart rate test is fairly straight forward. See the procedure. Blood Pressure: Blood pressure testing is a bit more complicated for people under the age of 18. Blood pressure is based on a student’s age and height. Height is the best indicator for growth. As th ...
... THEIR OWN VALUES ARE. Heart Rate: The heart rate test is fairly straight forward. See the procedure. Blood Pressure: Blood pressure testing is a bit more complicated for people under the age of 18. Blood pressure is based on a student’s age and height. Height is the best indicator for growth. As th ...
A single-headed fission yeast myosin V transports actin in a
... The separation of daughter cells at the end of mitosis depends on the function of the contractile ring, a structure that is conserved from yeast to animals. The fission yeast Schizosaccharomyces pombe is an excellent model system to study cytokinesis because it is genetically tractable and amenable ...
... The separation of daughter cells at the end of mitosis depends on the function of the contractile ring, a structure that is conserved from yeast to animals. The fission yeast Schizosaccharomyces pombe is an excellent model system to study cytokinesis because it is genetically tractable and amenable ...
Minguillon et al., 2005
... model using genetic techniques in the mouse, we analyzed the limb-type identity of the Prx1-Tbx4(4.48)-rescued limb buds and limbs. In addition to Tbx5, Tbx4, and Pitx1, certain Hox genes belonging to the HoxC cluster have a limb-type restricted expression pattern and have been implicated in limb-ty ...
... model using genetic techniques in the mouse, we analyzed the limb-type identity of the Prx1-Tbx4(4.48)-rescued limb buds and limbs. In addition to Tbx5, Tbx4, and Pitx1, certain Hox genes belonging to the HoxC cluster have a limb-type restricted expression pattern and have been implicated in limb-ty ...
From filaments to function: The role of the plant actin cytoskeleton in
... while further showing that actin was not required for the assembly of the viral glycoproteins with the nucleoprotein. Taken together, these studies clearly demonstrate a role for actin in the cellular trafficking of viral proteins during infection. This is noteworthy that while there are numerous exa ...
... while further showing that actin was not required for the assembly of the viral glycoproteins with the nucleoprotein. Taken together, these studies clearly demonstrate a role for actin in the cellular trafficking of viral proteins during infection. This is noteworthy that while there are numerous exa ...
Cdc6 in S phase in human cells - Journal of Cell Science
... Accumulated Cdc6 resisted extraction with detergent (+TX100 wash) showing that it binds to structures within the nucleus, probably chromatin. Furthermore, detergent extraction did not significantly decrease Cdc6 staining intensity, suggesting that all of the Cdc6 in the nucleus is chromatin bound. T ...
... Accumulated Cdc6 resisted extraction with detergent (+TX100 wash) showing that it binds to structures within the nucleus, probably chromatin. Furthermore, detergent extraction did not significantly decrease Cdc6 staining intensity, suggesting that all of the Cdc6 in the nucleus is chromatin bound. T ...
Assessing the Flexibility of Intermediate Filaments by Atomic Force
... form smooth-looking IFs 8 –12 nm in diameter (Figure 1).10 The mechanical properties of IF networks in vitro have mainly been studied by rheological and light scattering methods.3,11,12 From these studies it was concluded that individual IFs are relatively flexible polymers and that IF networks exhi ...
... form smooth-looking IFs 8 –12 nm in diameter (Figure 1).10 The mechanical properties of IF networks in vitro have mainly been studied by rheological and light scattering methods.3,11,12 From these studies it was concluded that individual IFs are relatively flexible polymers and that IF networks exhi ...
Worksheets - cloudfront.net
... Science rests on evidence and logic, so it deals only with things that can be observed. An observation is anything that is detected either through human senses or with instruments and measuring devices that extend human senses. Things that cannot be observed or measured by current means —such as sup ...
... Science rests on evidence and logic, so it deals only with things that can be observed. An observation is anything that is detected either through human senses or with instruments and measuring devices that extend human senses. Things that cannot be observed or measured by current means —such as sup ...
Interleukin 12 p40 Production by Barrier Epithelial Cells during
... chromogen. In these experiments, the BAL fluid (without concentration) was subjected to additional analyses of TNF-␣ levels by ELISA (with a sensitivity of 5 pg/ml) and IL-12 by Western blotting. For immunoblotting, cell-free BAL fluid was subjected to 10% PAGE under nonreducing or reducing (10 mM d ...
... chromogen. In these experiments, the BAL fluid (without concentration) was subjected to additional analyses of TNF-␣ levels by ELISA (with a sensitivity of 5 pg/ml) and IL-12 by Western blotting. For immunoblotting, cell-free BAL fluid was subjected to 10% PAGE under nonreducing or reducing (10 mM d ...
Sequestration of host metabolism by an intracellular pathogen | eLife
... host cell. Thus, C.trachomatis has developed ways to import these nutrients into inclusions, and, more generally, to take the control of its interactions with the host cell. The inclusions built up by C. trachomatis contain a high amount of glycogen, a carbohydrate that generally acts as an energy s ...
... host cell. Thus, C.trachomatis has developed ways to import these nutrients into inclusions, and, more generally, to take the control of its interactions with the host cell. The inclusions built up by C. trachomatis contain a high amount of glycogen, a carbohydrate that generally acts as an energy s ...
Direct Somatic Embryogenesis in Rice (Oryza sativa L - JMS
... garlic, but in another cultivar (A. sativum L., ‘White roppen’) through somatic embryogenesis5). It has been known that the genotype also influence the regeneration potential of cultured plant6,7). Therefore, we studied somatic embryogenesis of A. sativum L., cv. ‘Lumbu Hijau’. Somatic embryogenesis ...
... garlic, but in another cultivar (A. sativum L., ‘White roppen’) through somatic embryogenesis5). It has been known that the genotype also influence the regeneration potential of cultured plant6,7). Therefore, we studied somatic embryogenesis of A. sativum L., cv. ‘Lumbu Hijau’. Somatic embryogenesis ...
Characterization of Nifedipine Block of the Human Heart Delayed
... ological measurement of hKv1.5 currents have been described in detail previously (Fedida et al., 1993). Alternatively, hKv1.5 was transiently transfected into HEK cells using the mammalian expression vector pRc/CMV. Cells expressing hKv1.5 were detected by cotransfecting cells with the vector pHook- ...
... ological measurement of hKv1.5 currents have been described in detail previously (Fedida et al., 1993). Alternatively, hKv1.5 was transiently transfected into HEK cells using the mammalian expression vector pRc/CMV. Cells expressing hKv1.5 were detected by cotransfecting cells with the vector pHook- ...
Regulation of epidermal growth factor receptor signalling by
... signalling controls key cellular programmes, including survival, proliferation, differentiation and locomotion, both during development and post-natally (Sibilia et al., 2007). The execution of these diverse cellular programmes requires that receptor signals of adequate strength and duration are gen ...
... signalling controls key cellular programmes, including survival, proliferation, differentiation and locomotion, both during development and post-natally (Sibilia et al., 2007). The execution of these diverse cellular programmes requires that receptor signals of adequate strength and duration are gen ...