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Capping protein: new insights into mechanism
Capping protein: new insights into mechanism

... end [10] (Figure 2), and thus dominates the dynamics of filament assembly. In vivo barbed ends can be created when new filaments are nucleated by the Arp2/3 complex [5] or formins [12–14]. New barbed ends are often oriented toward a membrane [15], such that polymerization pushes the membrane forward ...
from dicp.ac.cn
from dicp.ac.cn

... CK2, in vitro kinase assays were carried out using a series of recombinant MSK1 proteins. MSK1 could be phosphorylated by CK2 in its C-terminal kinase domain. CK2α interacted only with the C-terminal kinase domain of MSK1, consistent with the location of the phosphorylated sequences. Five sites (Ser ...
Endocrine - Effingham County Schools
Endocrine - Effingham County Schools

... 3.Responding to stimuli 4.Control ...
DIFFERENT MODES OF FLUORESCENTLY LABELED
DIFFERENT MODES OF FLUORESCENTLY LABELED

... prostaglandins (4). It has been shown that the mechanism of glutamate and ANP release may involve exocytosis (3, 5-7), since these chemical messengers are found in membrane bound vesicles. In astrocytes these vesicles possess several components of SNARE molecules, key elements for vesicular exocytos ...
Structural features of the vesicle of Frankia sp. CpI1
Structural features of the vesicle of Frankia sp. CpI1

... cultures have been shown by 1 5 studies dinitrogen (Torrey et al. 1981). Vesicles formed in culture are globose, vary in size from 3 to 5 pm, and each is attached as a side branch of a filament with a vesicle stalk whose length is approximately equal to the vesicle diameter. In cultured Frankia, no ...
UvA-DARE (Digital Academic Repository) HIV
UvA-DARE (Digital Academic Repository) HIV

... for sensing and clearing pathogens, thus playing an important role in establishing primary as well as adaptive immune responses. These cells also contribute to regulation of tissue homeostasis, inflammation and repair1. Derived from a common myeloid progenitor, monocytes that originate in the bone m ...
Mitochondrial Antiviral Signaling Protein Innate Immunity by
Mitochondrial Antiviral Signaling Protein Innate Immunity by

DEVELOPMENT AND DISEASE Tbx5 is required for forelimb bud
DEVELOPMENT AND DISEASE Tbx5 is required for forelimb bud

... (A) Tbx5lox/lox;Prx1Cre pup viewed from the side. The forelimb has failed to form although the hindlimb has developed normally. (B) Ventral view of the pup shown in A. The arrow indicates a small flap of skin, present on one side of the embryo. (C) Tbx5 expression in the developing forelimb but not ...
The role of yolk syncytial layer and blastoderm movements during
The role of yolk syncytial layer and blastoderm movements during

... colleagues, who began genetic analysis in the zebrafish and established the methodological foundation allowing its use as a vertebrate model organism (Streisinger et al., 1981; Westerfield, 2000). Indeed, the zebrafish embryo offers many advantages to study embryonic development compared to other or ...
Polycystin-1 maturation requires polycystin-2 in a dose
Polycystin-1 maturation requires polycystin-2 in a dose

... with PC1-FL and PC1-CTS, as well as PC1-NTS via PC1-CTS/PC1NTS tethering, before PC1 maturation. Since PC2 was not affected by swainsonine and remained sensitive to EndoH, these data suggest that only PC1 passes through the Golgi, while PC2 is mostly retained in the ER. Localization of PC1 glycoform ...
PDF
PDF

... to the mesoderm (Moody, 1987). Sox2 expression was inhibited in descendants of these Syn4 MO-injected cells (Fig. 2C). This is specific for Syn4 because it could be rescued by co-injection of mRNA encoding a mutated Syn4 that does not bind to the MO (Fig. 2D). As an alternative approach, prospective ...
Biology I End-of-Course
Biology I End-of-Course

... on how quickly photosynthesis is occurring in each plant. B. The temperature of water in each aquarium This answer is not correct. The temperature of the water can change the rate of photosynthesis, but measuring the water temperature would not provide the most information on the rate of photosynthe ...
13-2-2014-chapter40Done
13-2-2014-chapter40Done

... • In phase I, renal blood flow and ureteral pressure rise together. • In phase II, the left renal blood flow begins to decline and ureteral pressure remains elevated and, in fact, continues to rise. • In phase III, the left renal blood flow and ureteral pressure decline together. ...
Leukotriene B4 Triggers the In Vitro and In Vivo
Leukotriene B4 Triggers the In Vitro and In Vivo

... 5-lipoxygenase and the leukotriene A4-hydrolase enzymes (for review see Ref. 22). LTB4 activates, through autocrine and paracrine mechanisms, several leukocyte types by binding and signaling through at least two distinct cell surface G protein-coupled receptors, BLT1 and BLT2 (23–25). The LTB4 recep ...
Stem Cell Teachers` Kit
Stem Cell Teachers` Kit

... Articles about stem cells regularly feature in Australian and global media. The science behind stem cell acquisition and use is commonly poorly understood by students and adults alike. This kit enables teachers to introduce and incorporate the concepts of stem cell science into their class rooms. Wh ...
Glencoe Biology
Glencoe Biology

... Chapter 32 Integumentary, Skeletal, and Muscular Systems Section 1: The Integumentary System Section 2: The Skeletal System Section 3: The Muscular System ...
TAL1occupancy_WuRevised_v16 - bx.psu.edu
TAL1occupancy_WuRevised_v16 - bx.psu.edu

... Unsupervised clustering by k-means (k=16) of the ChIP-seq signal strength at each TAL1 OS in the six cell types revealed the dynamics of TAL1 occupancy during differentiation (Fig. 2A). Very few TAL1 OSs were bound in all six cell types; this was estimated as 191 (1% of the total) based on the origi ...
Peptides that mimic Candida albicans–derived β
Peptides that mimic Candida albicans–derived β

... Chemical studies of C. albicans PPM have led to the identification of unusually linked mannose residues that are absent from S. cerevisiae PPM. These β-1,2-linked oligomannosides are present in the acid-stable fraction of C. albicans PPM as heteropolymers linked to the nonreducing terminal end of α- ...
View more Animal Life videos
View more Animal Life videos

... the trochophore larva of annelids, a single pole cell proliferates two strips of mesoblast lying on either side of the ventral midline. These bands subdivide transversely into bilateral solid blocks, the somites. Each somite then splits internally to form a hollow vesicle, the cavity of which is the ...
Regulation of nuclear poly(A) addition controls the expression of
Regulation of nuclear poly(A) addition controls the expression of

... In recent years, much work has focused on the regulation of cleavage ef®ciency at the secretory poly(A) site (Peterson and Perry, 1989; Peterson, 1992; EdwaldsGilbert and Milcarek, 1995; Phillips et al., 1996, 1999; Phillips and Virtanen, 1997; Martincic et al., 1998; Takagaki and Manley, 1998; Vera ...
Two lineage boundaries coordinate vertebrate apical ectodermal
Two lineage boundaries coordinate vertebrate apical ectodermal

... marked cell clusters examined, cells were generally found only within the AER and not also in the neighboring ectoderm. In a few exceptions, cells were seen in immediately adjacent ventral ectoderm (3/13) (Fig. 2H), and in one case in dorsal ectoderm (1/9). This suggested that additional borders mig ...
Interaction of Cationic Peptides with Lipoteichoic Acid and Gram
Interaction of Cationic Peptides with Lipoteichoic Acid and Gram

... LPS-induced toxicity in mice (8, 19, 22). In this study, we have investigated whether these synthetic ...
RENAL LYMPHANGIECTASIA. MDCT AND MRI FINDINGS
RENAL LYMPHANGIECTASIA. MDCT AND MRI FINDINGS

... More recently, a perinephric fluid accumulation has been described in a patient with established renal lymphangiectasia. The collection is not surrounded by a wall and does not appear like a cystic lesion, as is usually the case in renal lymphangiectasia. This finding has been explained by the conti ...
Influence of intercellular tissue connections on airway muscle
Influence of intercellular tissue connections on airway muscle

... tissue. Under a dissecting microscope, small strips of muscle tissue (,0.75 mm diameter and 8–12 mm long) were cut from the muscle sheet, with care being taken to follow the natural division of the tissue into discrete fiber bundles. To ensure a low-compliance (and removable) attachment to the exper ...
the uptake of valine and cytidine by sea
the uptake of valine and cytidine by sea

... cell division. A longer-term experiment with valine showed that the uptake rate remained approximately constant until the pluteus stage, and was not altered for the first l o h by a actinomycin D. Continuous labelling experiments indicated that the uptake rate may be controlled by the level of precu ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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