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Actin-Dependent and -Independent Functions of
Actin-Dependent and -Independent Functions of

... et al., 2009). Interestingly, the tor2 mutant shows right-handed cell expansion even in the single-celled leaf trichomes. In addition, the trichomes of tor2 are overbranched. This is consistent with the previous notion that microtubules have two functions in trichomes, namely, elongation and branchi ...
Visualization of APP dimerization and APP-Notch2 fluorescence complementation
Visualization of APP dimerization and APP-Notch2 fluorescence complementation

... together by the association of two interacting partners fused to the fragments. The advantages of BiFC analysis include: (i) the subcellular localization of protein interactions under conditions that closely reflect the normal physiological environment; (ii) BiFC fluorescence signals are easily detect ...
ARVO 2016 Annual Meeting Abstracts 163 Corneal Wound Repair
ARVO 2016 Annual Meeting Abstracts 163 Corneal Wound Repair

... cornea in a reproducible and targeted manner to aid the treatment of corneal endothelial dysfunction. Methods: A freezing console was designed and manufactured based on the use of nitrous oxide as a cryogen. The console was connected to one of four different cryoprobes, each with a different freezin ...
Pax1/Pax9 and vertebral column development
Pax1/Pax9 and vertebral column development

... as well as ossified fusions between vertebrae and neural arches are found (Fig. 1E,F). Thus, Pax1 and Pax9 interact during vertebral column development. This result is more clearly demonstrated in mutants that lack both copies of Pax1: in Pax1−/−; Pax9−/− double homozygous mutants, no vertebral bodi ...
C. elegans the L1 larval stage
C. elegans the L1 larval stage

... Axon guidance is the developmental process where developing neurons navigate their processes based on attractive and repulsive cues. C. elegans has been an instrumental model in the study of neurobiology with one of the key benefits being a relatively simple nervous system which comprises of only 30 ...
Gain-of-Function Mutations in SCN5A Gene Lead to Type
Gain-of-Function Mutations in SCN5A Gene Lead to Type

... SCN5A is a gain-of-function mutation producing late sodium current, INa,L. Brugada mutation in SCN5A is a loss-of-function causing INa decrease. Whereas, the mechanism for Dilated Cardiomyopathy mutations in SCN5A is still not fully understood. N1325S is one of the first series of mutations identifi ...
Actin Dynamics in Muscle Cells
Actin Dynamics in Muscle Cells

... generation of contraction. In non-muscle cells, actin filaments are very dynamic and regulated by an array of proteins that interact with actin filaments and/or monomeric actin. Interestingly, in non-muscle cells the barbed ends of the filaments are the predominant assembly place, whereas in muscle cel ...
the owner`s manual for the human body
the owner`s manual for the human body

... These germs live in a symbiosis with the nutritive medium that constitutes our body, allowing it to be built up and later decomposed, to be metamorphosed and recreated. These germs are pleiomorphic shape-shifters that are controlled by the medium in which they live. Germs are not something separate, ...
Excitatory Mechanisms in the Suprachiasmatic Nucleus: The Role of
Excitatory Mechanisms in the Suprachiasmatic Nucleus: The Role of

... Gillette 1997). Previous studies suggest that the basic mechanism responsible for the generation of these rhythms is intrinsic to individual cells in the SCN (Welsh et al. 1995) and perhaps in other cell types (Balsalobre et al. 1998). The core molecular mechanism driving these cellular oscillations ...
Physical and functional link of the leukemia
Physical and functional link of the leukemia

... gest that PML I could act as a mediator for AML1 and its coactivator p300/CBP to assemble into functional complexes and, consequently, activate AML1-dependent transcription and myeloid cell differentiation. (Blood. 2005;105:292-300) ...
Mathematical model predicts a critical role for osteoclast autocrine
Mathematical model predicts a critical role for osteoclast autocrine

... [11]. RANKL and osteoprotegerin (OPG) are critical regulators of bone resorption, that are expressed by osteoblasts and exhibit opposite effects on osteoclasts [12]. Whereas RANKL is a potent stimulator of osteoclasts, OPG prevents the interaction of RANKL with its receptor and inhibits bone resorpt ...
BIOACCUMULATION OF METAL CATIONS BY YEAST AND YEAST
BIOACCUMULATION OF METAL CATIONS BY YEAST AND YEAST

... The yeast biomass could be harvested after bioaccumulation by tangential filtration methods, or alternatively could be packed into hollow fibre microfilter membrane cartridges and used as a fixed-bed ...
Preview Sample 3
Preview Sample 3

...  Discuss the role of the nucleus in eukaryotic cells.  Describe the role of ribosomes in protein synthesis.  Identify the different parts of the endomembrane system.  Contrast the different functions of internal membranes and compartments.  Evaluate the importance of each step in the protei ...
pdf - European Association of Tissue Banks
pdf - European Association of Tissue Banks

... Remarkable developments in the scientific, technical and medical fields have led to the increased therapeutic use of human organs, tissues and cells. Transplantation of these substances of human origin (SOHO) has not only improved the quality of life of thousands of individuals but also prolonged th ...
Detection, Characterization, and Bioavailability of Membrane
Detection, Characterization, and Bioavailability of Membrane

... bioavailability for catalyzing peroxidation, we used phospholipid exchange protein to load membranes of intact erythrocytes (RBC) with - 2% phosphatidylethanolamine hydroperoxide (PEOOH) and monitored the development of peroxidation by-products during subsequent incubation. Normal RBC loaded with PE ...
Apical-basal patterning in Arabidopsis - Development
Apical-basal patterning in Arabidopsis - Development

... originates from descendants of both the apical and the basal daughter cell of the zygote. We have isolated a mutant of a new gene named BODENLOS (BDL) in which the primary root meristem is not formed whereas postembryonic roots develop and bdl seedlings give rise to fertile adult plants. Some bdl se ...
articles - Saleh Lab
articles - Saleh Lab

... organisms, such as Drosophila, certain cells can efficiently take up dsRNA but seem to be unable to transmit this dsRNA to other cells in the body4. dsRNA uptake without further transmission to other cells has also been reported for some mammalian cell types5–7. Other organisms (such as C. elegans o ...
Section Summaries With IPC Review • Concise two
Section Summaries With IPC Review • Concise two

... IPC TEKS 4 Force and Motion IPC 4A Motion, Work, and Power ...................................................................165 IPC 4B Newton’s Laws of Motion ...................................................................166 IPC 4D Efficiency of Machines ...................................... ...
Polarization of the endomembrane system is an early event in fucoid
Polarization of the endomembrane system is an early event in fucoid

... In most cell types examined, the actin cytoskeleton facilitates endocytosis [3,9]. The role of actin in polar endocytosis was examined by treatment with 30 nM Lat B, which depolymerizes actin filaments in S. compressa zygotes [8,15]. 6-h old zygotes were treated with Lat B for 1 h and then labeled w ...
Charakterisierung peroxisomaler und Lipid
Charakterisierung peroxisomaler und Lipid

... The peroxisomal and lipid droplets related proteins of yeast S. cerevisiae were characterized in this work. Lpx1p and Ldh1p are putative hydrolases and/or lipases of peroxisome and lipid droplets respectively; Pex1p and Pex6p are peroxisomal AAA ATPases; and Ubp15p is a deubiquitinating enzyme. It w ...
Proliferation and morphogenesis of the ER driven by the membrane
Proliferation and morphogenesis of the ER driven by the membrane

... independent transgenic lines expressing high levels of 1S:GFP in the whole plant (Fig. 2, ...
ABSTRACT Title of Document:
ABSTRACT Title of Document:

... and fatty acid metabolism to meet cellular energy and substrate needs for biosynthesis. The second objective was to determine the effects of glucose availability on thymocyte metabolism, energy status and survival. The hypothesis for this objective was that glucose availability induces changes in th ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... Extracellular cellulose microfibrils have also been proposed to play a role in the attachment of certain bacteria to plant cells (23, 26). The production of extracellular microfibrils by Agrobacterium tumefaciens leads to a firm cell-cell adhesion after its initial attachment to its plant host (23). ...
patterning mechanisms controlling vertebrate limb development
patterning mechanisms controlling vertebrate limb development

... are remarkably similar. Among the structures and organs mentioned above, the vertebrate limb bud is clearly an excellent experimental model to study the cellular and molecular mechanisms that regulate pattern formation during embryogenesis. Vertebrate limbs are appendages that perform crucial roles, ...
ExamView - SOL Review Life at the Systems and
ExamView - SOL Review Life at the Systems and

... In snapdragons, the combined expression of both alleles for flower color produces a new phenotype that is pink. This illustrates incomplete dominance. The Punnett square above shows that both the white and red snapdragons are homozygous. Which of the following would be the correct product from a cro ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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