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Glycoproteins with Type Common and Type Specific Antigenic Sites
... Cells and virus. BHK 2I cells were grown as described by Watson et al. 0966), in the Glasgow modification of Eagle's medium containing IO °/o (v/v) tryptose phosphate broth and 5 % (v/v) calf serum (ETC). Two HSV t strains, H F E M (Watson et al. 1966) and MPI7 (Brown et al. I973), and one HSV 2 str ...
... Cells and virus. BHK 2I cells were grown as described by Watson et al. 0966), in the Glasgow modification of Eagle's medium containing IO °/o (v/v) tryptose phosphate broth and 5 % (v/v) calf serum (ETC). Two HSV t strains, H F E M (Watson et al. 1966) and MPI7 (Brown et al. I973), and one HSV 2 str ...
Full Text - The International Journal of Developmental Biology
... But if Spemann realized the importance of induction by the upper lip, why did he wait six years after 1918 to complete and publish the upper lip grafts in marked donors and host? This is all the more puzzling when it is realized that he had already worked out the methods of interspecies grafting in ...
... But if Spemann realized the importance of induction by the upper lip, why did he wait six years after 1918 to complete and publish the upper lip grafts in marked donors and host? This is all the more puzzling when it is realized that he had already worked out the methods of interspecies grafting in ...
Composition and production of thiol constituents induced
... ECnG). Phytochelatins with nranging from 2 to 11 have been described [4,5]. Although glycine is the most common terminal amino acid, glutamic acid [6], serine [7], and βalanine [8] have been found instead of glycine in several species. Moreover, the terminal amino acid does not appear in desglycyl- ...
... ECnG). Phytochelatins with nranging from 2 to 11 have been described [4,5]. Although glycine is the most common terminal amino acid, glutamic acid [6], serine [7], and βalanine [8] have been found instead of glycine in several species. Moreover, the terminal amino acid does not appear in desglycyl- ...
Murine Regulatory T Cells Contain Hyperproliferative and Death
... published observations that Treg are “anergic” in vitro. However, if gated on live CD4+Foxp3+ Treg and CD4+Foxp32 Tcon (Fig. 1B, 1C), we found that Treg had undergone more cell divisions than Tcon, particularly when the stimulation was weak (Fig. 1D). Differential sensitivity to activation-induced d ...
... published observations that Treg are “anergic” in vitro. However, if gated on live CD4+Foxp3+ Treg and CD4+Foxp32 Tcon (Fig. 1B, 1C), we found that Treg had undergone more cell divisions than Tcon, particularly when the stimulation was weak (Fig. 1D). Differential sensitivity to activation-induced d ...
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... reasonable to expect that autophagy will have a significant impact on animal development, a process ultimately driven by changes in individual cell activity. Here, we discuss current concepts and recent findings regarding the cellular and developmental functions of autophagy. Although its role in de ...
... reasonable to expect that autophagy will have a significant impact on animal development, a process ultimately driven by changes in individual cell activity. Here, we discuss current concepts and recent findings regarding the cellular and developmental functions of autophagy. Although its role in de ...
khazar university human physiology
... Before publishing these lectures the author had to decide the question: is there any necessity to publish them, whereas there are many physiology textbooks, including those in English? The answer was positive, and here are the arguments for it. To begin with, we have the foreign editions in limited ...
... Before publishing these lectures the author had to decide the question: is there any necessity to publish them, whereas there are many physiology textbooks, including those in English? The answer was positive, and here are the arguments for it. To begin with, we have the foreign editions in limited ...
EGFR/Ras Signaling Controls Drosophila Intestinal Stem
... the EGFR, RAS or BRAF, but this has not been rigorously evaluated. During RNAi screening we discovered that depletion of Cic in Drosophila’s intestinal stem cells (ISCs) activates these cells for rampant proliferation [11]. Based on previous studies in other fly organs we hypothesized that Cic might ...
... the EGFR, RAS or BRAF, but this has not been rigorously evaluated. During RNAi screening we discovered that depletion of Cic in Drosophila’s intestinal stem cells (ISCs) activates these cells for rampant proliferation [11]. Based on previous studies in other fly organs we hypothesized that Cic might ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... Members of the Rho family of small GTPases have been shown to be important regulators of cellular behavior, especially actin cytoskeletal organization and acto-myosin based contractility (Hall, 1998). Rho proteins function as molecular switches, cycling between an active GTP-bound state and an inact ...
... Members of the Rho family of small GTPases have been shown to be important regulators of cellular behavior, especially actin cytoskeletal organization and acto-myosin based contractility (Hall, 1998). Rho proteins function as molecular switches, cycling between an active GTP-bound state and an inact ...
A bi-functional siRNA construct induces RNA
... enable future in vivo studies on the uptake, distribution and pharmacokinetics of siRNA, and is particularly important for the development of siRNA-based therapeutics. More generally, PCR-based detection of siRNA carries wide-ranging applications for RNAi reverse genetics. INTRODUCTION Synthetic sho ...
... enable future in vivo studies on the uptake, distribution and pharmacokinetics of siRNA, and is particularly important for the development of siRNA-based therapeutics. More generally, PCR-based detection of siRNA carries wide-ranging applications for RNAi reverse genetics. INTRODUCTION Synthetic sho ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
... involvement of a VSOAC in response to hypotonic stress comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullae ...
Outer Membrane Vesicle-Mediated Export of
... and PKD2) has not yet been fully investigated, although they have been suggested to be involved in protein-protein or protein-carbohydrate interaction [12]. Our previous studies provided a crystal structure model of the PKD1 domain from V. cholerae PrtV (residues 755– 838) and revealed a Ca++-bindin ...
... and PKD2) has not yet been fully investigated, although they have been suggested to be involved in protein-protein or protein-carbohydrate interaction [12]. Our previous studies provided a crystal structure model of the PKD1 domain from V. cholerae PrtV (residues 755– 838) and revealed a Ca++-bindin ...
The FTLD risk factor TMEM106B and MAP6 control dendritic
... Taken together, the late endosomal/lysosomal protein TMEM106B is required for proper maintenance of all except the primary dendrites possibly through a specific effect on lysosomes in the dendritic compartment. TMEM106B interacts with the microtubule-binding protein MAP6 in the brain In order to tie ...
... Taken together, the late endosomal/lysosomal protein TMEM106B is required for proper maintenance of all except the primary dendrites possibly through a specific effect on lysosomes in the dendritic compartment. TMEM106B interacts with the microtubule-binding protein MAP6 in the brain In order to tie ...
... proposal is that the rapid Golgi movement in plant cells allows the Golgi to continually collect vesicles budding from the ER (the vacuum cleaner model; Boevink et al., 1998). In this view, vesicles are thought to bud out from the ER, so Golgi bodies must continually move to collect them. A second m ...
Surge and destroy: the role of auxin in plant embryogenesis
... Induced genes also include many of the AUX/IAA genes, indicating the potential for a negative-feedback loop in which auxin action is self-limiting. As predicted by the model for auxin action described above, the levels of most auxin-regulated transcripts go up, but a sizable fraction of auxin-regula ...
... Induced genes also include many of the AUX/IAA genes, indicating the potential for a negative-feedback loop in which auxin action is self-limiting. As predicted by the model for auxin action described above, the levels of most auxin-regulated transcripts go up, but a sizable fraction of auxin-regula ...
Characterization of the Nucleolar Gene Product, Treacle, in Treacher Collins Syndrome
... amino acids shorter than its human ortholog, [35S]methionine- and [35S]cysteine-labeled mouse treacle migrated as a single band of ⬃220 kDa, confirming the aberrant mobility of treacle on SDS-PAGE. This unusual behavior was very reminiscent of the treacle-related protein Nopp140, which migrates as a ...
... amino acids shorter than its human ortholog, [35S]methionine- and [35S]cysteine-labeled mouse treacle migrated as a single band of ⬃220 kDa, confirming the aberrant mobility of treacle on SDS-PAGE. This unusual behavior was very reminiscent of the treacle-related protein Nopp140, which migrates as a ...
Identification of Owl Monkey CD4 Receptors Broadly Compatible
... HEK293T cells by cotransfecting 667 ng of Q23⌬EnvGFP (21) and 333 ng of the HIV-1 env clone of interest using Fugene 6 (Roche) transfection reagent at a ratio of 3 l of Fugene 6 to 1 g of DNA according to the manufacturer’s protocol. Generation of stable cell lines. HEK293T and Cf2Th/syn CCR5 (29) ...
... HEK293T cells by cotransfecting 667 ng of Q23⌬EnvGFP (21) and 333 ng of the HIV-1 env clone of interest using Fugene 6 (Roche) transfection reagent at a ratio of 3 l of Fugene 6 to 1 g of DNA according to the manufacturer’s protocol. Generation of stable cell lines. HEK293T and Cf2Th/syn CCR5 (29) ...
Anatomical characteristics of roots of citrus rootstocks that vary in
... among rootstocks. In contrast, in roots of 19-wk-old glasshouse plants generally 70–100% of the epidermis was still intact. There was no evidence of secondary xylem development in second-order fibrous roots in the field ; in seedling, pot-grown rootsystems, 75–97% of second-order roots had formed se ...
... among rootstocks. In contrast, in roots of 19-wk-old glasshouse plants generally 70–100% of the epidermis was still intact. There was no evidence of secondary xylem development in second-order fibrous roots in the field ; in seedling, pot-grown rootsystems, 75–97% of second-order roots had formed se ...
1 bio. hints of aipmt mt – viii
... 26. Ans. (c) Hint: Failure of cytokinesis after telophase results in polyploidy. In sickle cell anaemia, GAG is changed to GUG. 28. Ans. (c) Hint: Human genome contains 3164.7 million nucleotide bases. 33. Ans. (a) Hint: In Meselson & Stahl experiment the DNA extracted from the culture after one gen ...
... 26. Ans. (c) Hint: Failure of cytokinesis after telophase results in polyploidy. In sickle cell anaemia, GAG is changed to GUG. 28. Ans. (c) Hint: Human genome contains 3164.7 million nucleotide bases. 33. Ans. (a) Hint: In Meselson & Stahl experiment the DNA extracted from the culture after one gen ...
AcmA of Lactococcus lactis is an N-acetylglucosaminidase
... intracellular enzyme X-prolyl dipeptidyl aminopeptidase (PepX) was measured. After 60 h of incubation, PepX activity in the culture medium was also maximal in all samples, decreasing in all cases upon further incubation. Even though a considerable reduction in absorbance was obtained, hardly any Pep ...
... intracellular enzyme X-prolyl dipeptidyl aminopeptidase (PepX) was measured. After 60 h of incubation, PepX activity in the culture medium was also maximal in all samples, decreasing in all cases upon further incubation. Even though a considerable reduction in absorbance was obtained, hardly any Pep ...
Factors Involved in Fruit Calcium Deficiency Disorders
... C. Calcium Translocation to the Shoot After having been apoplastically or simplistically loaded into the xylem vessels in the root, Ca2þ is translocated to the shoot with the mass flow of water in response to the more negative water potentials developed in leaves and fruit triggered by transpiration ...
... C. Calcium Translocation to the Shoot After having been apoplastically or simplistically loaded into the xylem vessels in the root, Ca2þ is translocated to the shoot with the mass flow of water in response to the more negative water potentials developed in leaves and fruit triggered by transpiration ...
MAN-03609-001_003_02_Gen3+ 8 9 10 Elabeling
... tissue-electrode interface, or when the treatment timer reaches two minutes. Integral to the controller is the cavity integrity assessment system (CIA) which is designed to determine whether there is a defect or perforation in the wall of the uterus. After the disposable device is placed into the ut ...
... tissue-electrode interface, or when the treatment timer reaches two minutes. Integral to the controller is the cavity integrity assessment system (CIA) which is designed to determine whether there is a defect or perforation in the wall of the uterus. After the disposable device is placed into the ut ...
MAN-03523-001_007_02_Gen4 8 9 10_RF Controller
... tissue-electrode interface, or when the treatment timer reaches two minutes. Integral to the controller is the cavity integrity assessment system (CIA) which is designed to determine whether there is a defect or perforation in the wall of the uterus. After the disposable device is placed into the ut ...
... tissue-electrode interface, or when the treatment timer reaches two minutes. Integral to the controller is the cavity integrity assessment system (CIA) which is designed to determine whether there is a defect or perforation in the wall of the uterus. After the disposable device is placed into the ut ...
Actin-Dependent and -Independent Functions of
... et al., 2009). Interestingly, the tor2 mutant shows right-handed cell expansion even in the single-celled leaf trichomes. In addition, the trichomes of tor2 are overbranched. This is consistent with the previous notion that microtubules have two functions in trichomes, namely, elongation and branchi ...
... et al., 2009). Interestingly, the tor2 mutant shows right-handed cell expansion even in the single-celled leaf trichomes. In addition, the trichomes of tor2 are overbranched. This is consistent with the previous notion that microtubules have two functions in trichomes, namely, elongation and branchi ...