Identification of Pseudomonas aeruginosa flagellin as - AJP-Lung
... streptomycin (all from Life Technologies). P. aeruginosa binding assay. Adhesion of P. aeruginosa to transfected CHO cells was performed according to the method described by Rostand and Esko (40) and modified by Lillehoj et al. (24). Briefly, cells were cultured for 20–24 h in 24-well microplates in ...
... streptomycin (all from Life Technologies). P. aeruginosa binding assay. Adhesion of P. aeruginosa to transfected CHO cells was performed according to the method described by Rostand and Esko (40) and modified by Lillehoj et al. (24). Briefly, cells were cultured for 20–24 h in 24-well microplates in ...
K4 trimethylation PRMT6-mediated methylation of R2 in histone H3
... gene expression and in the transcriptional memory during cell division (Strahl and Allis 2000; Turner 2002). Depending on the nature and specific site of modifications, these histone marks determine either an active or repressed chromatin state (Jenuwein and Allis 2001). Recent genome-wide profiling ...
... gene expression and in the transcriptional memory during cell division (Strahl and Allis 2000; Turner 2002). Depending on the nature and specific site of modifications, these histone marks determine either an active or repressed chromatin state (Jenuwein and Allis 2001). Recent genome-wide profiling ...
Full-Text PDF
... The antiproliferative activity of ergosterol peroxide in the LS180 human adenocarcinoma cell line was assessed. In the first set of experiments, investigated compound at the concentration range (5–50 µg/mL) was applied and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was ...
... The antiproliferative activity of ergosterol peroxide in the LS180 human adenocarcinoma cell line was assessed. In the first set of experiments, investigated compound at the concentration range (5–50 µg/mL) was applied and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was ...
Apoptotic cell clearance: basic biology and therapeutic potential
... urate crystals, and interleukin‑33 (IL‑33). These endogenous molecules can be recognized variably by Toll-like receptors (TLRs), the C‑type lectin Mincle, receptor for advanced glycation end-products (RAGE) and ST2 (REFS 153,154). Interestingly, interaction of HMGB1 and HSP90 with CD24 on responding ...
... urate crystals, and interleukin‑33 (IL‑33). These endogenous molecules can be recognized variably by Toll-like receptors (TLRs), the C‑type lectin Mincle, receptor for advanced glycation end-products (RAGE) and ST2 (REFS 153,154). Interestingly, interaction of HMGB1 and HSP90 with CD24 on responding ...
The epithelial junction: bridge, gate, and fence.
... the question of the fence. The existence of a fence has been implicit in many discussions of epithelial physiology since 1950, but this problem has rarely been considered explicitly. Numerous studies have shown that the opposite-facing membranes of epithelial cells, the apical and basolateral membra ...
... the question of the fence. The existence of a fence has been implicit in many discussions of epithelial physiology since 1950, but this problem has rarely been considered explicitly. Numerous studies have shown that the opposite-facing membranes of epithelial cells, the apical and basolateral membra ...
Protein Sorting to the Storage Vacuoles of Plants: A Critical Appraisal
... VSR-PS1 from peas does not possess a dileucine motif, it does have a tyrosine-based sorting motif, YMPL, and this has recently been shown to interact with mA-adaptin, one of the five m-adaptins from Arabidopsis (25). As this adaptin is part of an AP1-like complex and localizes to the TGN (25), it in ...
... VSR-PS1 from peas does not possess a dileucine motif, it does have a tyrosine-based sorting motif, YMPL, and this has recently been shown to interact with mA-adaptin, one of the five m-adaptins from Arabidopsis (25). As this adaptin is part of an AP1-like complex and localizes to the TGN (25), it in ...
HOXs and lincRNAs: Two sides of the same coin
... transcriptional activity from both strands of coding and noncoding regions during development (19, 26–31). The functional significance of such noncoding transcription is beginning to emerge as evidence from several groups suggests the important roles of noncoding transcription in the regulation of H ...
... transcriptional activity from both strands of coding and noncoding regions during development (19, 26–31). The functional significance of such noncoding transcription is beginning to emerge as evidence from several groups suggests the important roles of noncoding transcription in the regulation of H ...
Supplemental Material_Tsang
... mmol) in ODE (2.5 ml) was swiftly injected into the flask under vigorous stirring with the protection of dry nitrogen. The reaction mixture was kept at 64-65 °C for 11 min and quenched by pouring the reaction solution into cold anhydrous 2-propanol (100 ml) in a dry-ice bath. The products were centr ...
... mmol) in ODE (2.5 ml) was swiftly injected into the flask under vigorous stirring with the protection of dry nitrogen. The reaction mixture was kept at 64-65 °C for 11 min and quenched by pouring the reaction solution into cold anhydrous 2-propanol (100 ml) in a dry-ice bath. The products were centr ...
Problems of cell death in neurodegeneration and Alzheimer`s Disease
... progressive diseases. In autopsy cases of AD, Parkinson’s disease (PD), related disorders, and age-matched controls, DNA fragmentation using the TUNEL method and an array of apoptosis-related proteins (ARP), proto-oncogenes, and activated caspase 3, the key enzyme of late-stage apoptosis, were exami ...
... progressive diseases. In autopsy cases of AD, Parkinson’s disease (PD), related disorders, and age-matched controls, DNA fragmentation using the TUNEL method and an array of apoptosis-related proteins (ARP), proto-oncogenes, and activated caspase 3, the key enzyme of late-stage apoptosis, were exami ...
Identification of Primary Lysosomes in Human
... other species.’9 Maturaincreasing nuclear poly- ...
... other species.’9 Maturaincreasing nuclear poly- ...
Muscle Tissue - El Camino College
... 5) ___ lines - protein discs where actin filaments attach; distance between Z lines _________ during contraction. ...
... 5) ___ lines - protein discs where actin filaments attach; distance between Z lines _________ during contraction. ...
University of Groningen Effects of structure, morphology and
... hour. Empty PVC loops were used as controls (Ctrl). The data collected from both donors were pooled. The y-axis of figure data also correlate with fibrinogen P2 exposure. ** p < 0.01. ...
... hour. Empty PVC loops were used as controls (Ctrl). The data collected from both donors were pooled. The y-axis of figure data also correlate with fibrinogen P2 exposure. ** p < 0.01. ...
THE GENE THE EVIDENCE OF THE NUCLEOPROTEIN NATURE OF
... conclusions can be accepted only as a first order approximation, and that much new data is necessary before we can exclude the possibility of specificities in the nucleic acids themselves. There is moreover a purely cytological difficulty which has not received attention. It is well known that the d ...
... conclusions can be accepted only as a first order approximation, and that much new data is necessary before we can exclude the possibility of specificities in the nucleic acids themselves. There is moreover a purely cytological difficulty which has not received attention. It is well known that the d ...
Nucleic Acid Synthesis in the Neoplastic Cell
... and animal cells (41, 92, 143, 253). It has been which are twined round one another. The two shown at the enzyme level that DNA polymerase chains are held together by hydrogen bonds be can incorporate the deoxyribonucleotide triphos- tween the bases, each base being joined to a com phates of several ...
... and animal cells (41, 92, 143, 253). It has been which are twined round one another. The two shown at the enzyme level that DNA polymerase chains are held together by hydrogen bonds be can incorporate the deoxyribonucleotide triphos- tween the bases, each base being joined to a com phates of several ...
ABSTRACT Title of Document:
... manipulated genetically using recombinant DNA technology, be directed for targeted differentiation and have a capacity for germline transmission. These qualities have made ESCs an excellent tool for genetic engineering (Capecchi 1989) by virtue of which they been used extensively in investigations o ...
... manipulated genetically using recombinant DNA technology, be directed for targeted differentiation and have a capacity for germline transmission. These qualities have made ESCs an excellent tool for genetic engineering (Capecchi 1989) by virtue of which they been used extensively in investigations o ...
Introduction to Endocrinology
... The endocrine hormones are carried by the circulatory system to cells throughout the body, including the nervous system in some cases, where they bind with receptors and initiate many reactions. Some endocrine hormones affect many different types of cells of the body; for example, growth hormone (fr ...
... The endocrine hormones are carried by the circulatory system to cells throughout the body, including the nervous system in some cases, where they bind with receptors and initiate many reactions. Some endocrine hormones affect many different types of cells of the body; for example, growth hormone (fr ...
Increased Expression of Cytoskeletal, Linkage, and Extracellular
... structure and functional integrity is well established.20 Tubulin is organized around the nucleus and in the longitudinal direction of the cell, and it contributes to the stability of the contractile apparatus in relation to the nucleus, mitochondria, and cellular membrane.21 Tubulin turnover is con ...
... structure and functional integrity is well established.20 Tubulin is organized around the nucleus and in the longitudinal direction of the cell, and it contributes to the stability of the contractile apparatus in relation to the nucleus, mitochondria, and cellular membrane.21 Tubulin turnover is con ...
Cell shape changes indicate a role for extrinsic tensile forces in
... qualitatively like a passive cellular material such as a foam, in which shape reflects stress16. Accordingly, different signatures are expected for pulls or pushes: if the tissue experiences a pull in one direction, it will tend to contract in the other two axes, leading to an increase in the cell p ...
... qualitatively like a passive cellular material such as a foam, in which shape reflects stress16. Accordingly, different signatures are expected for pulls or pushes: if the tissue experiences a pull in one direction, it will tend to contract in the other two axes, leading to an increase in the cell p ...
The Living World - Chapter 4 - McGraw Hill Higher Education
... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
A missense mutation in Caenorhabditis elegans prohibitin 2 confers
... Phb-2(ad2154) Worms Are Resistant to Other Drugs. We next determined the sensitivity of N2 worms to other tubulin-binding toxins, both microtubule stabilizers and destabilizers (Fig. 4) by treating L1 larvae with the drugs and scoring their ability to develop normally. Interestingly, compound 3 and ...
... Phb-2(ad2154) Worms Are Resistant to Other Drugs. We next determined the sensitivity of N2 worms to other tubulin-binding toxins, both microtubule stabilizers and destabilizers (Fig. 4) by treating L1 larvae with the drugs and scoring their ability to develop normally. Interestingly, compound 3 and ...
Myosin-Powered Membrane Compartment Drives Cytoplasmic
... much larger eukaryotic cells, where targeted transport of organelles and carrier vesicles, ER dynamics and cytosolic intermixing involve cytoskeletal transport networks encompassing microtubules and actin microfilaments with associated molecular motors [2–4]. Transport can be achieved by at least tw ...
... much larger eukaryotic cells, where targeted transport of organelles and carrier vesicles, ER dynamics and cytosolic intermixing involve cytoskeletal transport networks encompassing microtubules and actin microfilaments with associated molecular motors [2–4]. Transport can be achieved by at least tw ...
On the Morphology of the Drosophila Heart
... Abstract: The circulatory system of Drosophila melanogaster represents an easily amenable genetic model whose analysis at different levels, i.e., from single molecules up to functional anatomy, has provided new insights into general aspects of cardiogenesis, heart physiology and cardiac aging, to na ...
... Abstract: The circulatory system of Drosophila melanogaster represents an easily amenable genetic model whose analysis at different levels, i.e., from single molecules up to functional anatomy, has provided new insights into general aspects of cardiogenesis, heart physiology and cardiac aging, to na ...
Gene Section ADAM12 (ADAM metallopeptidase domain 12 (meltrin alpha))
... Structure of ADAM proteinase. ADAM-12 is composed of a propeptide (Pro), a metalloproteinase (Metallo), a disintegrin (Dis), a cysteinrich (Cysrich) domain followed by an EGF-like (EGF), a transmembrane (TM) and a cytoplasmic domain. ADAM-12 proteinase contains in addition a sequence recognized by f ...
... Structure of ADAM proteinase. ADAM-12 is composed of a propeptide (Pro), a metalloproteinase (Metallo), a disintegrin (Dis), a cysteinrich (Cysrich) domain followed by an EGF-like (EGF), a transmembrane (TM) and a cytoplasmic domain. ADAM-12 proteinase contains in addition a sequence recognized by f ...
Halloween genes encode P450 enzymes that mediate
... classical and molecular genetics. Flies and other insects grow by a series of molts in which a new and larger exoskeleton (cuticle) is synthesized by epidermal cells and the old cuticle is digested away and shed (ecdysis). Basically, the same process occurs in the metamorphosis of the larva (maggot) ...
... classical and molecular genetics. Flies and other insects grow by a series of molts in which a new and larger exoskeleton (cuticle) is synthesized by epidermal cells and the old cuticle is digested away and shed (ecdysis). Basically, the same process occurs in the metamorphosis of the larva (maggot) ...