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Pedomicrobium - International Journal of Systematic and
Pedomicrobium - International Journal of Systematic and

... acid) buffer (Sigma Chemie, Taufkirchen, Federal Republic of Germany), and growth in PSM supplemented with 10 mM phenol, 10 mM benzoic acid, or 10 mM toluene were followed optically by using a Klett-Summerson colorimeter. The generation times in PSM or PYVM medium were ...
Life Science - University of Texas Rio Grande Valley
Life Science - University of Texas Rio Grande Valley

... leadership,  and  collaborations  with  local  school  districts  and  governmental  agencies.  Faculty  members  are  also   encouraged  to  take  leadership  roles  in  societies  of  their  research  specialties.   ...
Hair Analysis
Hair Analysis

... In human hair, the color of the hair can make a difference too. Generally speaking, dark hair is thicker than blond hair and red hair is the finest. But then hair that has been colored artificially can give false clues. Hair is composed of two basic layers. The inner layer contains the pigment(s). M ...
Polyamines Regulate Growth Factor
Polyamines Regulate Growth Factor

... Cells were seeded at the density of 2×105 cells/ml in phenol red free DMEM supplemented with 2% FBS, which was pretreated with dextran-coated charcoal (DCC) to remove the endogenous hormone. After 72hr incubation, cultured cells were treated with 10 nM 17β-estradiol (E2), 5 ng/ml TGF-α, 5 ng/ml EGF, ...
Lessons from a gene regulatory network: echinoderm
Lessons from a gene regulatory network: echinoderm

... which, for example, TF-A provides a positive input into gene B, and TF-A and the TF encoded by gene B both provide positive inputs into gene C. Several instances of such feed-forward interactions are illustrated in Fig. 5 [for example, the positive input from ets1 (TF-A) to alx1 (gene B) is accompan ...
Isolation and purification of cell wall polysaccharide of Bacillus
Isolation and purification of cell wall polysaccharide of Bacillus

... polymer. I n d e e d , we are c u r r e n t l y e n g a g e d in the s e q u e n c i n g or structural analysis of the polysaccharide, the results of which will be p u b l i s h e d in the n e a r future. T h e overall study of the B. anthracis cell wall polysaccharide will certainly shed some light ...
Correlative Imaging of Fluorescent Proteins in Resin
Correlative Imaging of Fluorescent Proteins in Resin

... plant tissues. Thompson and Wolniak (2008) described the retention of mCitrine fused to an SEplasma membrane protein in glycol methacrylate sections. The fluorescent signal was stable using wide-field microscopy but bleached rapidly under the confocal microscope. To date, cryosections have been the pr ...
Chemosensory organs as models of neuronal synapses
Chemosensory organs as models of neuronal synapses

... developmental and functional importance of these cells at this information transfer site. Developmentally, glia-derived factors, including cholesterol74 and the secreted protein thrombospondin61, promote synapse formation, whereas the phagocytic functions of glia seem to participate in synaptic remo ...
Peeping in on the cytoskeleton: light microscopy
Peeping in on the cytoskeleton: light microscopy

... Labelled purified actin is the reagent of choice in in vitro single-molecule reconstitution experiments studying polymer growth properties or filament displacement (for example, by associated motor proteins11,12). Live cell imaging studies have also used fluorophore-labelled purified actin, but thes ...
Regular-spiking cells in the presubiculum are hyperexcitable in a rat
Regular-spiking cells in the presubiculum are hyperexcitable in a rat

... Origin (v. 8.6; OriginLab, Northampton, MA) with Ward’s method and squared Euclidean distances as the distance measure (Ward 1963). Briefly, neurons were sorted in a multidimensional space based on similarities of the electrophysiological variables considered. Clustering began with individual neuron ...
Identification of genes controlling germ cell migration and embryonic
Identification of genes controlling germ cell migration and embryonic

... proper differentiation of the midgut, as mutations in them cause a transformation of part of the PMG into a more hindgut-like tissue. In these mutants, the PMG intercellular gaps fail to form and germ cells are rendered helpless to reach their destination in the mesoderm (Jaglarz and Howard, 1995; R ...
ERK Nuclear Translocation Is Dimerization
ERK Nuclear Translocation Is Dimerization

... piggyback mechanism via binding to nuclear localization sequence-containing proteins (5). Nuclear localization sequencedependent mechanisms require energy for Ran-dependent cycling of importins (15). However, reconstituted import assays have shown that ERK can bind directly to FXFG sequences of nucl ...
Chemosensory organs as models of neuronal synapses
Chemosensory organs as models of neuronal synapses

... important roles in defining and positioning synaptic sites during development in C. elegans76 and have been reported to regulate dendritic spine shape in the mouse through ephrin signalling77. Although the functions of glia at synapses are not fully understood, they have been implicated in the regul ...
Functions of the Cdc14-Family Phosphatase Clp1p in the Cell Cycle
Functions of the Cdc14-Family Phosphatase Clp1p in the Cell Cycle

Inflammation and colorectal cancer - Tel
Inflammation and colorectal cancer - Tel

... have an increased risk of developing colorectal cancer (CRC). However, the underlying mechanisms are not entirely clear. A genetic basis for the increased risk of CRC in IBD patients is only a partial explanation. It is possible that high levels of inflammatory mediators that are produced in this se ...
Heterodimerization and Endocytosis of Arabidopsis
Heterodimerization and Endocytosis of Arabidopsis

... findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Sacco C. de Vries ([email protected]). Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.104.025387. ...
Increased root hair density by loss of WRKY6 in Arabidopsis
Increased root hair density by loss of WRKY6 in Arabidopsis

... are found in different species, with the model plant Arabidopsis thaliana having a simple, regular pattern ideally suited for genetic dissection of the underlying genetics (Datta et al., 2011). Root hairs provide a competitive advantage to Arabidopsis thaliana (Bates & Lynch, 2001) and barley (Gahoo ...
Neural reflexes in inflammation and immunity
Neural reflexes in inflammation and immunity

... other soluble mediators normally balance the immune system. Work dating back to 1961 demonstrated that electrically stimulating the vagus nerve significantly enhanced the release of acetylcholine from the spleen (Brandon and Rand, 1961; Leaders and Dayrit, 1965). By then, anatomy studies had already ...
Nucleolus: the fascinating nuclear body
Nucleolus: the fascinating nuclear body

... UBF, close to Wbrillarin-containing caps. In the central body are proteins derived from the GC, some of which are progressively released, such as PM-Scl 100. It was recently demonstrated that certain nucleolar caps of segregated nucleoli could recruit factors involved in mRNA splicing. In this case, ...
Islet Amyloid Polypeptide Is a Target Antigen for
Islet Amyloid Polypeptide Is a Target Antigen for

... sequence of secreted IAPP is quite similar between mouse and human forms of the molecule, and there is only one amino acid difference between the KS20 mouse and human sequences. It is not unreasonable, therefore, to assume that human CD4 T cells also may react to IAPP peptides presented, for example ...
- Mochtar Riady Institute
- Mochtar Riady Institute

... Generation of monocyte-derived dendritic cell (MDDC) MDDC were generated as described previously [17] with some modifications. In brief, PBMC were adhered to 12 well culture plates with density 1 x 106 ml-1 for 30 min at 37°C with 5% CO2. The non adherent cells were removed by gentle wash and the ad ...
Cell dynamics of folding in two
Cell dynamics of folding in two

... In this paper, we focus on the two-dimensional model of a protein consisting of a 16 monomer self-avoiding chain on a square lattice [17,18]. The total number of conformations is only 802 075, of which 69 are maximally compact (henceforth we denote the maximally compact conformation as a cell), i.e. ...
Lymphadenosis Benigna Cutis or Cutaneous Lymphoid Hyperplasia
Lymphadenosis Benigna Cutis or Cutaneous Lymphoid Hyperplasia

... long- term antigenic stimulation are implicated in many cases.[5] Other causes include arthropod bite, borrelia infection, as a postzoster phenomenon[6];in HIV infected individuals[7] or after tattoos, exposure to gold and nickel, vaccinations, and taking drugs such as antihistaminics and phenytoin. ...
Material ecologies for synthetic biology
Material ecologies for synthetic biology

... the morphology and behavior of biological systems is derived from groups of different genes being expressed through the more complex (compared to the genome) proteome—the entire population of proteins produced by a cell or organism at particular growth stages or in particular environments [4]. This ...
Full_CAD_Artical - Synbio.Construction
Full_CAD_Artical - Synbio.Construction

... the morphology and behavior of biological systems is derived from groups of different genes being expressed through the more complex (compared to the genome) proteome—the entire population of proteins produced by a cell or organism at particular growth stages or in particular environments [4]. This ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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