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Interplay between cell growth and cell cycle in
Interplay between cell growth and cell cycle in

... oscillates, while M-phase CDK activity is kept low. In mammals and insects, the S-phase CDK complex Cyclin E–Cdk2 plays a central role in the endocycle. As mentioned above, plants do not appear to have Cyclin E, so they rely instead on Cyclin D–CDK complexes to progress through S-phase during the en ...
Microsporidia: Why Make Nucleotides if You Can Steal Them?
Microsporidia: Why Make Nucleotides if You Can Steal Them?

... Microsporidia and the recycling of nucleotides The continual turnover of RNA, which releases the nucleoside monophosphates, represents a major, ready-made source of nucleotides [27]. The enzymes needed to regenerate nucleoside triphosphates following nucleic acid degradation have been retained by mi ...
From spores to antibiotics via the cell cycle
From spores to antibiotics via the cell cycle

... Our main interest was in understanding the basis for the generation of asymmetry and the specification of the separate fates of the prespore and mother cell, because these seemed to represent the most basic and fundamental questions posed by the system. Observations in various laboratories had shown ...
The roles of microtubules in tropisms
The roles of microtubules in tropisms

... investigated in any further detail [1]. Gravitropism and phototropism have received the most attention, although some studies addressing hydrotropism and thigmotropism have been published in recent years [2–10]. Tropisms can be either positive or negative depending on whether growth occurs towards o ...
Message in a bottle: small signalling peptide
Message in a bottle: small signalling peptide

... small signalling peptides. First, as receptors commonly form complexes, it may be that interacting proteins are able to alter the conformation of the receptor, allowing a single receptor protein to perceive multiple ligands and to activate multiple, distinct signalling cascades based on alternative ...
The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to
The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to

... 2003). Analysis of secondary structure and local compositional complexity also suggests that the C-terminal region of Mps3 (amino acids 436–682) folds into a discrete globular domain, which has been shown by deletion analysis to be important for Mps3 function (Tables S2 and S3, available at http://w ...
Chapter 31
Chapter 31

... a. Septate hyphae - Most fungi have hyphae divided into cells by septa (crosswalls) with large pores for sharing material b. Coencytic hyphae – continuous cytoplasm with many, many nuclei due to repeated mitosis without cytokinesis similar to slime molds. ...
Fine-mapping quantitative trait loci affecting murine external ear
Fine-mapping quantitative trait loci affecting murine external ear

... External ear hole closure in LG/J mice represents a model of regenerative response. It is accompanied by the formation of a blastema-like structure and the re-growth of multiple tissues, including cartilage. The ability to regenerate tissue is heritable. An F34 advanced intercross line of mice (Wust ...
Characterization of Pinin, A Novel Protein Associated with the
Characterization of Pinin, A Novel Protein Associated with the

... and (c) an acidic domain rich in glutamic acid. Although the 3' end of the open reading frame of the clone for pinin showed near identity to a partial cDNA isolated for a pig neutrophil phosphoprotein (Bellavite, P., F. Bazzoni, et al. 1990. Biochem. Biophys. Res. Commun. 170:915-922), the remaining ...
The journey of developing hematopoietic stem cells
The journey of developing hematopoietic stem cells

... by the regulation of HSC self-renewal and differentiation. This is possible because the bone marrow contains specialized niches in which the multipotency of HSCs is conserved through cell divisions, while their progeny are directed towards lineage differentiation (Wilson and Trumpp, 2006). During ho ...
From milliseconds to millions of years: guard cells and
From milliseconds to millions of years: guard cells and

... Other wax mutants also lend support to the idea that disruption of the extracellular matrix leads to a change in stomatal index: eceriferum-1 and eceriferum-6 mutants of Arabidopsis [20], both of which have alterations in wax composition on the leaf surface, exhibit higher stomatal indices than do w ...
Control of pathfinding by the avian trunk neural crest
Control of pathfinding by the avian trunk neural crest

... the interface between the dermamyotome and sclerotome and migrate along this interface until they reach the dorsal aorta (Fig. IB; Loring & Erickson, 1987). Only later are crest cells found in the sclerotome. These later-appearing crest cells may detach from the dermamyotome and entering the sclerot ...
The Use of Conditional Lethal Cell Cycle Mutants for
The Use of Conditional Lethal Cell Cycle Mutants for

... cycle during which the gene product in question is normally synthesized. [Note that some gene products that function during discrete intervals of the cycle may be synthesized continuously throughout the cycle, while others (as shown in fig. 3) may be synthesized shortly before their times of functio ...
Test Bank - rsffa.org
Test Bank - rsffa.org

... ____ 14. A cell in a multicellular organism must be able to carry out all of the functions necessary for life in order for the organism to survive. _________________________ ____ 15. There is a division of labor among the cells of multicellular organisms. _________________________ Completion Complet ...
The TOR signalling network from yeast to man
The TOR signalling network from yeast to man

... yTORC1 is regulated by the abundance and/or quality of the available nitrogen source. Similarly, starving mammalian cells of (especially branched-chain) amino acids, results in mTORC1 inhibition. However, it is not known how nutrient abundance/quality is sensed and how this information is transmitte ...
Mochii - Salamander Genome Project
Mochii - Salamander Genome Project

... urodele limb regeneration. Notochord precursor cells accumulate to create a compact cell mass adjacent to the edge of the amputated notochord sheath (Fig. 1B). The cells proliferate and align along the proximal-to-distal direction to make an immature notochord (Fig. 1C), which continues to elongate ...
PDF
PDF

... development, we carried out quantitative RT-PCR (qRT-PCR) for all known ligands able to activate the BMP receptors (Mueller and Nickel, 2012). We tested both retina and the retinal pigment epithelium (RPE), as previous studies have shown the RPE to be a source of several members of the Bmp family (B ...
SNARE Complex Proteins in Rat Mast Cells Phosphorylation of
SNARE Complex Proteins in Rat Mast Cells Phosphorylation of

... triggered by Fc␧RI cross-linking. Leukocyte chemoattractant receptors functionally couple to heterotrimeric G proteins and can trigger PLC␤ activation either directly (through the Gq family of G␣ proteins) or via the released G␤␥ proteins (16). Chemoattractant-induced activation of PLC␤ leads to acc ...
Identification of Pseudomonas aeruginosa flagellin as - AJP-Lung
Identification of Pseudomonas aeruginosa flagellin as - AJP-Lung

... streptomycin (all from Life Technologies). P. aeruginosa binding assay. Adhesion of P. aeruginosa to transfected CHO cells was performed according to the method described by Rostand and Esko (40) and modified by Lillehoj et al. (24). Briefly, cells were cultured for 20–24 h in 24-well microplates in ...
Mode Suppressed TEM Cell Design For High Frequency IC
Mode Suppressed TEM Cell Design For High Frequency IC

... order TE or TM modes should be suppressed, but the TEM mode and the shielding efficiency should not be affected. Several different methods have been introduced on suppressing higher order mode resonances in a large TEM cell [6-8]. All those methods were found effective to dampen resonances, but all ...
Role of Polo-like kinase in the degradation of early mitotic inhibitor 1
Role of Polo-like kinase in the degradation of early mitotic inhibitor 1

... purified by affinity chromatography on glutathione-agarose. Both proteins were ⬎95% homogeneous, as judged by SDS兾 PAGE and Coomassie staining. Assay of the Ligation of Emi1 to Ubiquitin by SCF␤-TrCP. Reaction mixtures in a volume of 10 ␮l contained 40 mM Tris䡠HCl, pH 7.6, 2 mg兾ml ovalbumin (carrier ...
Powerpoint template for scientific poster
Powerpoint template for scientific poster

... Galvani in the late 1700s (Adler and Shi 1988). Galvanotaxis, one aspect of this control, is the change in cellular movement created by an electrical field and has been an object of study for many years. This migration occurs in the direction of either the cathode (negative pole) or the anode (posit ...
Complementary Signaling Pathways Regulate the Unfolded Protein
Complementary Signaling Pathways Regulate the Unfolded Protein

... a reducing reagent that disrupts disulfide bond formation in the ER. Northern blot and quantitative Taqman RT-PCR analysis showed that in mixed stage worms grown in liquid culture, expression of both hsp-3 and hsp-4 increased with time, and reached a plateau at 4–6 hr (Figures 1A and 1B). At the pla ...
10 Proteomic Analysis of Potential Breast Cancer Biomarkers
10 Proteomic Analysis of Potential Breast Cancer Biomarkers

... responsible for growth control, cell adhesion/migration, matrix-degradation, invasion and angiogenesis (Mbeunkui et al., 2006b). Importantly, these tumor cell secreted proteins majorly enter body fluid system such as blood, urine, lymph fluid and can be measured by non-invasive tests. Thus, analysis ...
Forage digestibility: the intersection of cell wall lignification and plant
Forage digestibility: the intersection of cell wall lignification and plant

... readers that detergent data suffer from known short comings. Cellulose is the single most abundant component in cell walls and composed exclusively of linear glucose chains. The other wall polysaccharides are categorized into two groups, hemicellulose and pectin, that are characterized by extraction ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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