Minus End-Directed Kinesin-Like Motor Protein
... However, some meiotic and early embryonic cells lack typical centrosomes. Mouse oocytes have pericentriolar material but lack centrioles [reviewed in Balczon, 1996]. Furthermore, it has been shown that Drosophila oocytes do not have a centrosome in any form [Matthies et al., 1996]. However, in proph ...
... However, some meiotic and early embryonic cells lack typical centrosomes. Mouse oocytes have pericentriolar material but lack centrioles [reviewed in Balczon, 1996]. Furthermore, it has been shown that Drosophila oocytes do not have a centrosome in any form [Matthies et al., 1996]. However, in proph ...
Lipid-Protein Bio-Nanotubes with Open or Closed Ends
... the weakly positive charged vesicles (‘beads’) adsorbed onto the negatively charged microtubule, MT, wall (‘rod’), forming the beads on a rod, BOR (Figure 1, lower right). Bridges are occasionally formed between adjacent MTs forming small sized bundles of MTs cross-linked via vesicles (Figure 1, low ...
... the weakly positive charged vesicles (‘beads’) adsorbed onto the negatively charged microtubule, MT, wall (‘rod’), forming the beads on a rod, BOR (Figure 1, lower right). Bridges are occasionally formed between adjacent MTs forming small sized bundles of MTs cross-linked via vesicles (Figure 1, low ...
The Ndc80p Complex from Saccharomyces cerevisiae Contains
... spindle length (estimated as the distance between the nuclear edges of the two central plaques), it was necessary for the spindle to be in, or very close to, the plane of the section. In addition, all single SPBs (n ⫽ 30), some of which may have been produced by breakup of longer spindles, had stain ...
... spindle length (estimated as the distance between the nuclear edges of the two central plaques), it was necessary for the spindle to be in, or very close to, the plane of the section. In addition, all single SPBs (n ⫽ 30), some of which may have been produced by breakup of longer spindles, had stain ...
Volume 94, issue 2 July 2011 ...
... induce axonal elongation. In the context of this problem the work from Smith’s group is particularly interesting. In an effort to design strategies for improved axonal regeneration following injury, they developed a specialized chamber system in which neurons are cultured on two initially contiguous ...
... induce axonal elongation. In the context of this problem the work from Smith’s group is particularly interesting. In an effort to design strategies for improved axonal regeneration following injury, they developed a specialized chamber system in which neurons are cultured on two initially contiguous ...
The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape
... those of pollen tubes. In general, microtubule-disrupting drugs block the initiation of polarized growth, and actin filament inhibitors mainly affect the maintenance of polarized growth (Tiwari and Wilkins, 1995; Mathur et al., 1999; Szymanski, 2000). The requirements for both microtubules and actin ...
... those of pollen tubes. In general, microtubule-disrupting drugs block the initiation of polarized growth, and actin filament inhibitors mainly affect the maintenance of polarized growth (Tiwari and Wilkins, 1995; Mathur et al., 1999; Szymanski, 2000). The requirements for both microtubules and actin ...
Modelling the structural response of an eukaryotic cell in the optical
... Modelling fibroblasts in the stretcher Analytic thick shell elastic model for actin cortex: Ample experimental evidence, albeit more indirect and qualitative, shows that out of the three cytoskeletal polymers, actin dominates the cell’s structural response5–8. Hence, in our first approach to model a ...
... Modelling fibroblasts in the stretcher Analytic thick shell elastic model for actin cortex: Ample experimental evidence, albeit more indirect and qualitative, shows that out of the three cytoskeletal polymers, actin dominates the cell’s structural response5–8. Hence, in our first approach to model a ...
SUSPENSOR DEVELOPMENT IN GAGEA LUTEA (L.) KER GAWL
... forms a compaction of actin material is also present in older basal cells (4-cell proembryo stage, Fig. 3a,b). Microfilament bundles arranged circumferentially around the nucleus are visible in the basal cell cytoplasm. The actin skeleton elements are oriented transversely to the micropylarchalazal ...
... forms a compaction of actin material is also present in older basal cells (4-cell proembryo stage, Fig. 3a,b). Microfilament bundles arranged circumferentially around the nucleus are visible in the basal cell cytoplasm. The actin skeleton elements are oriented transversely to the micropylarchalazal ...
In Vitro Assays Demonstrate That Pollen Tube Organelles Use
... ability to glide microtubules in the in vitro motility assay, for the presence of microtubule-stimulated ATPase activity, and for cross-reactivity with anti-kinesin antibodies. We identified and characterized a 105-kD organelle-associated motor protein that is functionally, biochemically, and immuno ...
... ability to glide microtubules in the in vitro motility assay, for the presence of microtubule-stimulated ATPase activity, and for cross-reactivity with anti-kinesin antibodies. We identified and characterized a 105-kD organelle-associated motor protein that is functionally, biochemically, and immuno ...
Identification of Isoforms of a Mitotic Motor in Mammalian Spermatogenesis
... protein found in the testes. The complete sequence of the KIFC5A cDNA is homologous to a group of carboxyl-terminal motors, including hamster CHO2, human HSET, and mouse KIFC1 and KIFC4. The KIFC5A and KIFC1 cDNAs are nearly identical except for the presence of two additional sequence blocks in the ...
... protein found in the testes. The complete sequence of the KIFC5A cDNA is homologous to a group of carboxyl-terminal motors, including hamster CHO2, human HSET, and mouse KIFC1 and KIFC4. The KIFC5A and KIFC1 cDNAs are nearly identical except for the presence of two additional sequence blocks in the ...
mutant alleles of polymitotic that disrupt the cell cycle
... nuclear morphology is abnormal in these cells. The chromatin is more diffuse than that in wild-type cells at a similar stage (compare to Fig. 1C). In Fig. 2B, two tetrad cells show abnormal interphase nuclear microtubule arrays. However, the other two cells are separated by a phragmoplast. In wild-t ...
... nuclear morphology is abnormal in these cells. The chromatin is more diffuse than that in wild-type cells at a similar stage (compare to Fig. 1C). In Fig. 2B, two tetrad cells show abnormal interphase nuclear microtubule arrays. However, the other two cells are separated by a phragmoplast. In wild-t ...
Increased Expression of Cytoskeletal, Linkage, and Extracellular
... representative lanes are shown in Figures 2, 3, 5, 6, and 7. E and F, Immunofluorescence for ␣-tubulin (green). E, In normal myocardium, the myocytes are regularly arranged and the tubulin fluorescence is present as a fine network in each cell. F, In failing myocardium, the tubulin fluorescence is m ...
... representative lanes are shown in Figures 2, 3, 5, 6, and 7. E and F, Immunofluorescence for ␣-tubulin (green). E, In normal myocardium, the myocytes are regularly arranged and the tubulin fluorescence is present as a fine network in each cell. F, In failing myocardium, the tubulin fluorescence is m ...
Structural and Functional Implications of an Unusual Foraminiferal b
... the conventional view of MT disassembly may not hold in these organisms. Foraminiferan tubulin can exist in two distinct assembled forms: conventional 13-protofilament MTs and helical filaments (HFs), which are two globular subunits thick and 10.7 6 0.7 nm wide (HFs, see fig. 1) (Hauser and Schwab 1 ...
... the conventional view of MT disassembly may not hold in these organisms. Foraminiferan tubulin can exist in two distinct assembled forms: conventional 13-protofilament MTs and helical filaments (HFs), which are two globular subunits thick and 10.7 6 0.7 nm wide (HFs, see fig. 1) (Hauser and Schwab 1 ...
Lecture 06, case study - Taxol - Cal State LA
... 1964: Extract of Pacfic Yew bark showed anti-tumor activity, but limited supply of bark (endangered tree) delayed isolation of the active component 1971: Taxol identified as the anti-tumor molecule in Yew bark, but not explored as a drug for a further 12 years ...
... 1964: Extract of Pacfic Yew bark showed anti-tumor activity, but limited supply of bark (endangered tree) delayed isolation of the active component 1971: Taxol identified as the anti-tumor molecule in Yew bark, but not explored as a drug for a further 12 years ...
Neuronal polarity: an evolutionary perspective
... expect these would be lost once minus-end-out microtubules were no longer present. Indeed, ribosomes and membranes that were thought to be Golgi were reduced by antisense oligonucleotides targeting kinesin-6 (Yu et al., 2000). The poorly understood tapering shape of dendrites also became more unifor ...
... expect these would be lost once minus-end-out microtubules were no longer present. Indeed, ribosomes and membranes that were thought to be Golgi were reduced by antisense oligonucleotides targeting kinesin-6 (Yu et al., 2000). The poorly understood tapering shape of dendrites also became more unifor ...
The FRA1 kinesin contributes to cortical microtubule
... Copyright © 2015 American Society of Plant Biologists. All rights reserved. ...
... Copyright © 2015 American Society of Plant Biologists. All rights reserved. ...
Chapter 7. The Cell: Cytoskeleton
... Proteins that make up the fibers are very similar in all living things ...
... Proteins that make up the fibers are very similar in all living things ...
Paclitaxel-induced microtubule stabilization causes mitotic block
... cell might have to be increased to such a level as to completely shut down microtubule dynamics. Apoptosis, a natural process of programmed cell death that is required for the proper development of multicellular organisms, is induced by a number of stress-producing treatments, including anti-mitotic ...
... cell might have to be increased to such a level as to completely shut down microtubule dynamics. Apoptosis, a natural process of programmed cell death that is required for the proper development of multicellular organisms, is induced by a number of stress-producing treatments, including anti-mitotic ...
Nuclear Envelope Breakdown Proceeds by
... 2000), and together with in vivo observations suggest that the nuclear periphery is a highly crosslinked and stable protein network (Daigle et al., 2001). In dividing cells, this network is abolished during NE breakdown (NEBD), which defines the transition from prophase to prometaphase in the cell d ...
... 2000), and together with in vivo observations suggest that the nuclear periphery is a highly crosslinked and stable protein network (Daigle et al., 2001). In dividing cells, this network is abolished during NE breakdown (NEBD), which defines the transition from prophase to prometaphase in the cell d ...
Axon and dendritic trafficking
... neuron with a versatile mechanism to rapidly tune single synapse function to changing needs. Previous work has demonstrated that a number of AZ molecules including Piccolo, Bassoon and ELKS-2/ CAST, are carried as preassembled complexes to presynaptic sites by Piccolo-Bassoon transport vesicles (PTV ...
... neuron with a versatile mechanism to rapidly tune single synapse function to changing needs. Previous work has demonstrated that a number of AZ molecules including Piccolo, Bassoon and ELKS-2/ CAST, are carried as preassembled complexes to presynaptic sites by Piccolo-Bassoon transport vesicles (PTV ...
-Tubulin Plays an Essential Role in the Coordination of Mitotic Events
... Nuclei exit mitosis quickly even if mitosis has not been completed successfully. In addition, mipAD159 accelerates mitotic exit even if microtubules are eliminated by benomyl. These results indicate that ␥-tubulin has an important function in the coordination of late mitotic events and that it has a ...
... Nuclei exit mitosis quickly even if mitosis has not been completed successfully. In addition, mipAD159 accelerates mitotic exit even if microtubules are eliminated by benomyl. These results indicate that ␥-tubulin has an important function in the coordination of late mitotic events and that it has a ...
Regulation of microtubule dynamics by microtubule
... predominantly found in neurons, and are thought to control microtubule dynamics in vivo. There is a major group of microtubule-interacting proteins that have ATPase activity and transiently bind to tubulin. These «motor» proteins are involved in the transport of organelles along microtubules. As the ...
... predominantly found in neurons, and are thought to control microtubule dynamics in vivo. There is a major group of microtubule-interacting proteins that have ATPase activity and transiently bind to tubulin. These «motor» proteins are involved in the transport of organelles along microtubules. As the ...
Poster
... Alzheimer’s involves hyperphosphorylation, the addition of more than one phosphate group, to tau, an intracellular microtubule protein. Thrombin is a serine protease involved in the final step of blood coagulation and is normally present in the brain’s neurons, where it cleaves tau. It is necessary ...
... Alzheimer’s involves hyperphosphorylation, the addition of more than one phosphate group, to tau, an intracellular microtubule protein. Thrombin is a serine protease involved in the final step of blood coagulation and is normally present in the brain’s neurons, where it cleaves tau. It is necessary ...
Untitled - University of Guelph
... conveys the fact that, during development, one region of a cell grows differently from another, but it raises the question of how preferences for localized growth can be created within a cell. Furthermore, what molecular hierarchy might operate between cellular components for differential growth to ...
... conveys the fact that, during development, one region of a cell grows differently from another, but it raises the question of how preferences for localized growth can be created within a cell. Furthermore, what molecular hierarchy might operate between cellular components for differential growth to ...
Microtubule Reconfiguration during Axonal Retraction Induced by
... The prevailing view is that cytoskeletal elements depolymerize extensively during physiological axonal retraction (Song and Poo, 1999). This view is largely based on pharmacological studies showing that wholesale microtubule depolymerization can indeed cause axons to retract (Yamada et al., 1970; Da ...
... The prevailing view is that cytoskeletal elements depolymerize extensively during physiological axonal retraction (Song and Poo, 1999). This view is largely based on pharmacological studies showing that wholesale microtubule depolymerization can indeed cause axons to retract (Yamada et al., 1970; Da ...
Microtubule
Microtubules (micro- + tube + -ule) are a component of the cytoskeleton, found throughout the cytoplasm. These tubular polymers of tubulin can grow as long as 50 micrometres and are highly dynamic. The outer diameter of a microtubule is about 24 nm while the inner diameter is about 12 nm. They are found in eukaryotic cells and are formed by the polymerization of a dimer of two globular proteins, alpha and beta tubulin.Microtubules are very important in a number of cellular processes. They are involved in maintaining the structure of the cell and, together with microfilaments and intermediate filaments, they form the cytoskeleton. They also make up the internal structure of cilia and flagella.They provide platforms for intracellular transport and are involved in a variety of cellular processes, including the movement of secretory vesicles, organelles, and intracellular macromolecular assemblies (see entries for dynein and kinesin). They are also involved in chromosome separation (mitosis and meiosis), and are the major constituents of mitotic spindles, which are used to pull apart eukaryotic chromosomes.Microtubules are nucleated and organized by microtubule organizing centers (MTOCs), such as the centrosome found in the center of many animal cells or the basal bodies found in cilia and flagella, or the spindle pole bodies found in fungi.There are many proteins that bind to microtubules, including the motor proteins kinesin and dynein, severing proteins like katanin, and other proteins important for regulating microtubule dynamics.