Cell cycle - GEOCITIES.ws
... Sometimes the cells exit the cell cycle (usually from G1 phase) and enter the G0 phase. In the G0 phase, cells are alive and metabolically active, but do not divide. In this phase cells do not copy their DNA and do not prepare for cell division. Many cells in the human body, including those in heart ...
... Sometimes the cells exit the cell cycle (usually from G1 phase) and enter the G0 phase. In the G0 phase, cells are alive and metabolically active, but do not divide. In this phase cells do not copy their DNA and do not prepare for cell division. Many cells in the human body, including those in heart ...
Functional coupling of microtubules to membranes
... for maintaining a juxtanuclear Golgi network, a highly distributed but dynamic endoplasmic reticulum (ER) or specifically positioned endosomal compartments. We do know, however, that this organization is largely governed by the cytoskeleton. The microtubule and actin networks both have substantial r ...
... for maintaining a juxtanuclear Golgi network, a highly distributed but dynamic endoplasmic reticulum (ER) or specifically positioned endosomal compartments. We do know, however, that this organization is largely governed by the cytoskeleton. The microtubule and actin networks both have substantial r ...
tubulin isotypes - Journal of Cell Science
... benD, that appeared to cosegregate with some of the previously mentioned ¡3-tubulin restriction fragments in meiotic analysis. Mutations in ben A cosegregate with the bet A locus and mutations in benD cosegregate with the betB locus. Thus, the general conclusion of this study is the suggestion that ...
... benD, that appeared to cosegregate with some of the previously mentioned ¡3-tubulin restriction fragments in meiotic analysis. Mutations in ben A cosegregate with the bet A locus and mutations in benD cosegregate with the betB locus. Thus, the general conclusion of this study is the suggestion that ...
Cytoskeletal control of plant cell shape: getting the fine points
... new cell wall material and membrane [29]. However, Factin has long been suspected to play one or more additional roles in tip growth. This notion has recently been strengthened by the observation that tip growth is considerably more sensitive to a variety of actin-disrupting treatments than is the ...
... new cell wall material and membrane [29]. However, Factin has long been suspected to play one or more additional roles in tip growth. This notion has recently been strengthened by the observation that tip growth is considerably more sensitive to a variety of actin-disrupting treatments than is the ...
Small and stable peptidic PEGylated quantum dots to - HAL
... diameter of ~10 nm.36 Based on these results, we prepared small QDs based on peptide ND, composed of a tricysteine adhesive group, a hexamer of PEG and an aspartic acid grafted to the terminal group. We show that for these QD-ND, the PEG spacer effectively prevents nonspecific adsorption on cell mem ...
... diameter of ~10 nm.36 Based on these results, we prepared small QDs based on peptide ND, composed of a tricysteine adhesive group, a hexamer of PEG and an aspartic acid grafted to the terminal group. We show that for these QD-ND, the PEG spacer effectively prevents nonspecific adsorption on cell mem ...
Force is a signal that cells cannot ignore
... called the biomembrane force probe, can also be used to apply forces to protein–protein interactions; however, most investigators have moved toward the laser trapping, AFM, or magnetic beads platforms (Evans et al, 1995; Gourier et al., 2008; Neuman and Nagy, 2008). Proteins are typically tethered ...
... called the biomembrane force probe, can also be used to apply forces to protein–protein interactions; however, most investigators have moved toward the laser trapping, AFM, or magnetic beads platforms (Evans et al, 1995; Gourier et al., 2008; Neuman and Nagy, 2008). Proteins are typically tethered ...
Team Publications
... disrupts human brain development. Doublecortin stabilizes microtubules and stimulates their polymerization but has no homology with other MAPs. We used electron microscopy to characterize microtubule binding by doublecortin and visualize its binding site. Doublecortin binds selectively to 13 protofil ...
... disrupts human brain development. Doublecortin stabilizes microtubules and stimulates their polymerization but has no homology with other MAPs. We used electron microscopy to characterize microtubule binding by doublecortin and visualize its binding site. Doublecortin binds selectively to 13 protofil ...
Molecular encounters at microtubule ends in the plant cell cortex
... at MT plus ends via interaction with the first ones. Owing to their specific localization, +TIPs influence MT dynamics, and also enable the growing plus end to search for specific cellular targets like organelles, membranes, microfilaments or other MTs. In animals, these proteins have been involved ...
... at MT plus ends via interaction with the first ones. Owing to their specific localization, +TIPs influence MT dynamics, and also enable the growing plus end to search for specific cellular targets like organelles, membranes, microfilaments or other MTs. In animals, these proteins have been involved ...
Nucleation and Dynamics of Golgi-derived Microtubules
... MT-binding proteins and/or local tubulin dimer concentration at the Golgi helps to overcome kinetic barriers at the initial nucleation step. According to our model, emerging MTs are subsequently stabilized by Golgi-associated MT-stabilizing proteins. We discuss molecular factors potentially involved ...
... MT-binding proteins and/or local tubulin dimer concentration at the Golgi helps to overcome kinetic barriers at the initial nucleation step. According to our model, emerging MTs are subsequently stabilized by Golgi-associated MT-stabilizing proteins. We discuss molecular factors potentially involved ...
Repeat motifs of tau bind to the insides of microtubules in the
... Fig. 3. (A) Western blots of SDS gels, with lanes containing undigested tubulin (Tu) and subtilisin-digested tubulin (ST), incubated with four different anti-tubulin monoclonal antibodies speci®c to either a- or b-tubulin; the epitopes lie either at the very C-termini or closer to the N-termini. (B) ...
... Fig. 3. (A) Western blots of SDS gels, with lanes containing undigested tubulin (Tu) and subtilisin-digested tubulin (ST), incubated with four different anti-tubulin monoclonal antibodies speci®c to either a- or b-tubulin; the epitopes lie either at the very C-termini or closer to the N-termini. (B) ...
The Type II Arabidopsis Formin14 Interacts with Microtubules and
... characterization of nerve growth cone guidance (Tanaka et al., 1995; Dent and Gertler, 2003). In plant cells, microtubules and microfilaments are often codistributed in the cortical area in interphase cells (Blancaflor, 2000) and colocalize in structures, such as the preprophase band, mitotic spindl ...
... characterization of nerve growth cone guidance (Tanaka et al., 1995; Dent and Gertler, 2003). In plant cells, microtubules and microfilaments are often codistributed in the cortical area in interphase cells (Blancaflor, 2000) and colocalize in structures, such as the preprophase band, mitotic spindl ...
γ-Tubulin Is Essential for Microtubule Organization and
... 3 weeks after germination. This partially viable mutant combination enabled us to follow dynamically the effects of g-tubulin depletion on microtubule arrays in dividing cells using a green fluorescent protein marker. These results establish the central role of g-tubulin in the formation and organiz ...
... 3 weeks after germination. This partially viable mutant combination enabled us to follow dynamically the effects of g-tubulin depletion on microtubule arrays in dividing cells using a green fluorescent protein marker. These results establish the central role of g-tubulin in the formation and organiz ...
Memoryless self-reinforcing directionality in endosomal active
... the transport restarts on another microtubule. This is reasonable, as the cargo may approach the microtubule in various different orientations, and the distribution of the motors on the cargo surface is expected to be heterogeneous29 . Our numerical simulations based on this model, specified in Meth ...
... the transport restarts on another microtubule. This is reasonable, as the cargo may approach the microtubule in various different orientations, and the distribution of the motors on the cargo surface is expected to be heterogeneous29 . Our numerical simulations based on this model, specified in Meth ...
The Plant Cytoskeleton: Vacuoles and Cell Walls Make the Difference
... do not abolish cell division can develop surprisingly normally. Arabidopsis fass/ton plants fail to establish normal cortical interphase arrays and PPBs, and consequently display severe defects in polar cell growth and cell plate positioning (Traas et al., 1995). These mutant plants remain highly st ...
... do not abolish cell division can develop surprisingly normally. Arabidopsis fass/ton plants fail to establish normal cortical interphase arrays and PPBs, and consequently display severe defects in polar cell growth and cell plate positioning (Traas et al., 1995). These mutant plants remain highly st ...
Imaging cytoskeletal filament organization at the molecular scale
... tools to investigate the organization of septins in cells, which has not been funded yet. It involves new developments on customized protein constructs (I. Fresnel, molecular biology, biochemistry), on septin isoformspecific cell reagents (CRCM), and on optical imaging developments (I. Fresnel) in a ...
... tools to investigate the organization of septins in cells, which has not been funded yet. It involves new developments on customized protein constructs (I. Fresnel, molecular biology, biochemistry), on septin isoformspecific cell reagents (CRCM), and on optical imaging developments (I. Fresnel) in a ...
Regulation of tubulin heterodimer partitioning during interphase and
... first required for segregation of duplicated chromosomes, and has subsequently evolved to facilitate complex cell shapes and evolution of multi-cellular organisms. The cytoskeleton encompasses microtubules, intermediate filaments and actin filaments (also known as microfilaments), which are polymers ...
... first required for segregation of duplicated chromosomes, and has subsequently evolved to facilitate complex cell shapes and evolution of multi-cellular organisms. The cytoskeleton encompasses microtubules, intermediate filaments and actin filaments (also known as microfilaments), which are polymers ...
Running Head: Superresolution of cortical
... at video rates (Marc et al., 1998; Shaw et al., 2013). However, the bulk of plant cells organized ...
... at video rates (Marc et al., 1998; Shaw et al., 2013). However, the bulk of plant cells organized ...
The TACC proteins: TACC-ling microtubule dynamics and
... dynamic nature of the cytoskeletal components. Microtubules are dynamic filaments with fundamental roles in eukaryotic cell organization and function. During cell division, they form the bipolar spindle, which segregates the chromosomes into the two daughter cells. Microtubules show prolonged states ...
... dynamic nature of the cytoskeletal components. Microtubules are dynamic filaments with fundamental roles in eukaryotic cell organization and function. During cell division, they form the bipolar spindle, which segregates the chromosomes into the two daughter cells. Microtubules show prolonged states ...
Cell Structure and Function
... • Mitochondria and chloroplasts • Independent and self-sufficient ...
... • Mitochondria and chloroplasts • Independent and self-sufficient ...
Functions of the Arabidopsis kinesin superfamily of microtubule
... potentially could contribute to bundling of CMTs. However, the atk5 mutant has normal CMT organization (Ambrose et al. 2005), indicating that it does not play an essential role in CMT organization. CMTs are dynamic at both ends and their dynamic properties are important for array organization (Shaw ...
... potentially could contribute to bundling of CMTs. However, the atk5 mutant has normal CMT organization (Ambrose et al. 2005), indicating that it does not play an essential role in CMT organization. CMTs are dynamic at both ends and their dynamic properties are important for array organization (Shaw ...
Molecular Motors
... • Nerve impulses reaching the muscle produce an "action potential" that spreads over the sarcolemmal membrane and into the fiber along the t-tubule network • The signal is passed across the triad junction and induces release of Ca2+ ions from the SR • Ca2+ ions bind to sites on the fibers and induce ...
... • Nerve impulses reaching the muscle produce an "action potential" that spreads over the sarcolemmal membrane and into the fiber along the t-tubule network • The signal is passed across the triad junction and induces release of Ca2+ ions from the SR • Ca2+ ions bind to sites on the fibers and induce ...
Mechanism of Inhibition of Cell Proliferation by
... Fig. 2. Concentration dependence for inhibition of cell proliferation, for frequency of metaphase arrest, and for inhibition of the metaphase/anaphase transition after incubation of HeLa cells with vincristine, vinblastine, vindesine, vinepidine, and vinrosidine. The percent inhibition of cell proli ...
... Fig. 2. Concentration dependence for inhibition of cell proliferation, for frequency of metaphase arrest, and for inhibition of the metaphase/anaphase transition after incubation of HeLa cells with vincristine, vinblastine, vindesine, vinepidine, and vinrosidine. The percent inhibition of cell proli ...
Sample Textbook
... Microtubules are also important in moving genetic material during cell division. Microtubules make up the main structure of centrioles. Centrioles are found only in animal cells, and some fungi and algae. There are no centrioles in plants. Centrioles are important in cell division, where they help t ...
... Microtubules are also important in moving genetic material during cell division. Microtubules make up the main structure of centrioles. Centrioles are found only in animal cells, and some fungi and algae. There are no centrioles in plants. Centrioles are important in cell division, where they help t ...
Plant RanGAPs are localized at the nuclear envelope in interphase
... 2000) in plant cells. However, to date the characterization of genes coding for other key elements postulated to be involved in these processes, such as (i) the Ran accessory proteins RanGAP and RanGEF (RCC1); and (ii) proteins de®ning the structure of the nuclear pore complex, have remained elusive ...
... 2000) in plant cells. However, to date the characterization of genes coding for other key elements postulated to be involved in these processes, such as (i) the Ran accessory proteins RanGAP and RanGEF (RCC1); and (ii) proteins de®ning the structure of the nuclear pore complex, have remained elusive ...
Microtubule
Microtubules (micro- + tube + -ule) are a component of the cytoskeleton, found throughout the cytoplasm. These tubular polymers of tubulin can grow as long as 50 micrometres and are highly dynamic. The outer diameter of a microtubule is about 24 nm while the inner diameter is about 12 nm. They are found in eukaryotic cells and are formed by the polymerization of a dimer of two globular proteins, alpha and beta tubulin.Microtubules are very important in a number of cellular processes. They are involved in maintaining the structure of the cell and, together with microfilaments and intermediate filaments, they form the cytoskeleton. They also make up the internal structure of cilia and flagella.They provide platforms for intracellular transport and are involved in a variety of cellular processes, including the movement of secretory vesicles, organelles, and intracellular macromolecular assemblies (see entries for dynein and kinesin). They are also involved in chromosome separation (mitosis and meiosis), and are the major constituents of mitotic spindles, which are used to pull apart eukaryotic chromosomes.Microtubules are nucleated and organized by microtubule organizing centers (MTOCs), such as the centrosome found in the center of many animal cells or the basal bodies found in cilia and flagella, or the spindle pole bodies found in fungi.There are many proteins that bind to microtubules, including the motor proteins kinesin and dynein, severing proteins like katanin, and other proteins important for regulating microtubule dynamics.