Introduction to Course and Cell Cycle - March 21
... less tightly coiled. • Cytokinesis, division of the cytoplasm, begins. Fig. 12.5f ...
... less tightly coiled. • Cytokinesis, division of the cytoplasm, begins. Fig. 12.5f ...
A New Role for a Long-Studied DNA
... microtubule-kinetochore attachments. In control cells, Aurora B localizes to the inner centromere when the chromosomes attach to their spindles and then migrates toward the middle of the spindle poles as the chromatids are pulled apart. The researchers conclude that by detaching DNA tangles, TOPO II ...
... microtubule-kinetochore attachments. In control cells, Aurora B localizes to the inner centromere when the chromosomes attach to their spindles and then migrates toward the middle of the spindle poles as the chromatids are pulled apart. The researchers conclude that by detaching DNA tangles, TOPO II ...
Plant cell division is specifically affected by nitrotyrosine
... a general effect of nitrosylated amino acids, but rather is specific for NO2Tyr. For a deeper insight, the MIs of rice root tips and tobacco BY-2 were determined. Figure 2C shows the increase of mitotic inhibition for rice root tips either after 6 d of continuous cultivation (filled diamonds) on NO2 ...
... a general effect of nitrosylated amino acids, but rather is specific for NO2Tyr. For a deeper insight, the MIs of rice root tips and tobacco BY-2 were determined. Figure 2C shows the increase of mitotic inhibition for rice root tips either after 6 d of continuous cultivation (filled diamonds) on NO2 ...
Lecture 4
... • are variable in length but can grow 1000 times as long as they are thick • are built by the assembly of dimers of alpha tubulin and beta tubulin. • are found in both animal and plant cells ...
... • are variable in length but can grow 1000 times as long as they are thick • are built by the assembly of dimers of alpha tubulin and beta tubulin. • are found in both animal and plant cells ...
The Cell
... • Boundary between the cell and the environment • “Gatekeeper” of the cell • Maintains different conditions inside and outside of the cell • Allows materials to enter and exit ...
... • Boundary between the cell and the environment • “Gatekeeper” of the cell • Maintains different conditions inside and outside of the cell • Allows materials to enter and exit ...
7.06 Cell Biology QUIZ #2
... meiotic nondisjunction? Explain your reasoning for your choice or choices. i.) the proteins of the pre-RC break down over time ii.) securin is not stable over prolonged periods iii.) the microtubules of the spindle have a short half life iv.) the cohesin complex breaks down ...
... meiotic nondisjunction? Explain your reasoning for your choice or choices. i.) the proteins of the pre-RC break down over time ii.) securin is not stable over prolonged periods iii.) the microtubules of the spindle have a short half life iv.) the cohesin complex breaks down ...
The Arabidopsis sku6-spiral1 gene encodes a plus end
... a Multigene Family The T-DNA element responsible for the spr1-6 mutation was inserted at codon 75 within the second exon of a three-exon gene (At2g03680) predicted to encode a novel 119–amino acid polypeptide (12 kD; Figures 3A and 3B). BLAST searches of GenBank identified no SPR1 homologs outside o ...
... a Multigene Family The T-DNA element responsible for the spr1-6 mutation was inserted at codon 75 within the second exon of a three-exon gene (At2g03680) predicted to encode a novel 119–amino acid polypeptide (12 kD; Figures 3A and 3B). BLAST searches of GenBank identified no SPR1 homologs outside o ...
Notes: Cell Division & the Cell Cycle (Ch. 12)
... (DNA) before they divide, ensuring that each daughter cell receives an exact copy of the genetic material ● A dividing cell duplicates its DNA, allocates the two copies to opposite ends of the cell, and only then splits into ...
... (DNA) before they divide, ensuring that each daughter cell receives an exact copy of the genetic material ● A dividing cell duplicates its DNA, allocates the two copies to opposite ends of the cell, and only then splits into ...
Product information: SiR-tubulin Kit (CY-SC002)
... Note: This protocol was optimized using human fibroblast cells adhering to coverslips and has been confirmed in other common cell lines. Recommendations for experimental protocols should be used as a starting point, and optimal labeling conditions for each cell type should be determined empirically. ...
... Note: This protocol was optimized using human fibroblast cells adhering to coverslips and has been confirmed in other common cell lines. Recommendations for experimental protocols should be used as a starting point, and optimal labeling conditions for each cell type should be determined empirically. ...
CENP-E Is a Plus End–Directed Kinetochore Motor Required for
... been whether kinetochore-associated microtubule motors play an important role in congression. Using immunodepletion from and antibody addition to Xenopus egg extracts, we show that the kinetochore-associated kinesin-like motor protein CENP-E is essential for positioning chromosomes at the metaphase ...
... been whether kinetochore-associated microtubule motors play an important role in congression. Using immunodepletion from and antibody addition to Xenopus egg extracts, we show that the kinetochore-associated kinesin-like motor protein CENP-E is essential for positioning chromosomes at the metaphase ...
THE CELL CYCLE AND MITOSIS UNIT 3 ORGANIZATION AND
... • Mitosis makes new cells for repair; to replace old, damaged, or dead cells. • Mitosis makes new cells for growth. • Somatic (non sex cells) undergo mitosis. • In every mitotic division, 2 cells are made. • These cells are genetically identical to the parent cell. ...
... • Mitosis makes new cells for repair; to replace old, damaged, or dead cells. • Mitosis makes new cells for growth. • Somatic (non sex cells) undergo mitosis. • In every mitotic division, 2 cells are made. • These cells are genetically identical to the parent cell. ...
Ciliary Microtubule Capping Structures Contain A
... affinity-purified antibodies were tested by immunoblotting as shown in Fig. 4 (lanes 3 and 3') and found to bind exclusively to the 97-kD protein in the cap fraction and to no proteins in the axonemal fraction. The 34-kD polypeptide that occasionally stained in the capping or axonemal fraction was n ...
... affinity-purified antibodies were tested by immunoblotting as shown in Fig. 4 (lanes 3 and 3') and found to bind exclusively to the 97-kD protein in the cap fraction and to no proteins in the axonemal fraction. The 34-kD polypeptide that occasionally stained in the capping or axonemal fraction was n ...
Chromosomes - Spokane Public Schools
... (DNA) before they divide, ensuring that each daughter cell receives an exact copy of the genetic material ● A dividing cell duplicates its DNA, allocates the two copies to opposite ends of the cell, and only then splits into ...
... (DNA) before they divide, ensuring that each daughter cell receives an exact copy of the genetic material ● A dividing cell duplicates its DNA, allocates the two copies to opposite ends of the cell, and only then splits into ...
Microtubules and Microfilaments in Cell
... lack well-defined MTOCs and the rapid reorganization and reorientation of microtubules in their absence still remains one of the most intriguing areas of plant cytoskeletal research [37]. One way by which new ends, each capable of fresh polymerization and depolymerization, can be generated is by cut ...
... lack well-defined MTOCs and the rapid reorganization and reorientation of microtubules in their absence still remains one of the most intriguing areas of plant cytoskeletal research [37]. One way by which new ends, each capable of fresh polymerization and depolymerization, can be generated is by cut ...
MICROTUBULE ORGANIZATION 1 Regulates
... 48 h revealed incomplete cell walls, wall stubs, and, occasionally, internal wall fragments or inclusions (data not shown). To confirm that these aberrant formations were part of the cell wall, we labeled transverse and longitudinal sections of root tips with an antibody specific for xyloglucan, the ...
... 48 h revealed incomplete cell walls, wall stubs, and, occasionally, internal wall fragments or inclusions (data not shown). To confirm that these aberrant formations were part of the cell wall, we labeled transverse and longitudinal sections of root tips with an antibody specific for xyloglucan, the ...
Vacuoles - SCHOOLinSITES
... Receives protein-filled vesicles that bud from the ER. Vesicles fuse with membrane of Golgi apparatus. ...
... Receives protein-filled vesicles that bud from the ER. Vesicles fuse with membrane of Golgi apparatus. ...
chapter 2
... The cytoskeleton is composed of microfilaments, intermediate filaments, and microtubules. Microfilaments are composed of thin protein filaments (actin proteins) organized into two intertwined strands. The maintain cell shape, support cell shape changes, help muscles contract, separate the two cells ...
... The cytoskeleton is composed of microfilaments, intermediate filaments, and microtubules. Microfilaments are composed of thin protein filaments (actin proteins) organized into two intertwined strands. The maintain cell shape, support cell shape changes, help muscles contract, separate the two cells ...
Neuronal cell biology, polarity, subcellular specializatio…
... its axon. Mutant E3(504-509AAAAAA) with a six-alanine replacement in the apical sorting domain is present in both axons and dendrites (C), as is EAAT2 (D). Arrows indicate axons; arrowheads indicate dendrites. Scale bar, 100 µm. [Better experiment would be to double label for specific markers of axo ...
... its axon. Mutant E3(504-509AAAAAA) with a six-alanine replacement in the apical sorting domain is present in both axons and dendrites (C), as is EAAT2 (D). Arrows indicate axons; arrowheads indicate dendrites. Scale bar, 100 µm. [Better experiment would be to double label for specific markers of axo ...
Document
... The cell wall is an extracellular structure that distinguishes plant cells from animal cells Prokaryotes, fungi, and some unicellular eukaryotes also have cell walls The cell wall protects the plant cell, maintains its shape, and prevents excessive uptake of water Plant cell walls are made o ...
... The cell wall is an extracellular structure that distinguishes plant cells from animal cells Prokaryotes, fungi, and some unicellular eukaryotes also have cell walls The cell wall protects the plant cell, maintains its shape, and prevents excessive uptake of water Plant cell walls are made o ...
TKRP125, a kinesin-related protein involved in the centrosome
... that UV irradiation of the equatorial region of Haemanthus endosperm cells resulted in the disruption of phragmoplast microtubules that extended from the irradiated site. The phragmoplast in plasma membrane-permeabilized endosperm cells of Haemanthus incorporated exogenously applied tubulin at the e ...
... that UV irradiation of the equatorial region of Haemanthus endosperm cells resulted in the disruption of phragmoplast microtubules that extended from the irradiated site. The phragmoplast in plasma membrane-permeabilized endosperm cells of Haemanthus incorporated exogenously applied tubulin at the e ...
distribution of microtubules in the golgi apparatus of euglena gracilis
... 500-ml Erlenmeyer flasks containing 100 ml of media, in the dark, and without shaking. They were exposed to room illumination for about 1-2 h prior to being fixed for microscopy. Cells were prefixed in 005 M sec-collidine-buffered glutaraldehyde (2 %)-paraformaldehyde (2 %) for 45 min, rinsed well i ...
... 500-ml Erlenmeyer flasks containing 100 ml of media, in the dark, and without shaking. They were exposed to room illumination for about 1-2 h prior to being fixed for microscopy. Cells were prefixed in 005 M sec-collidine-buffered glutaraldehyde (2 %)-paraformaldehyde (2 %) for 45 min, rinsed well i ...
Mitosis What is (and is not) mitosis?
... interphase is technically not part of mitosis, but rather encompasses stages G1, S, and G2 of the cell cycle. Interphase The cell is engaged in metabolic activity and performing its prepare for mitosis (the next four phases that lead up to and include nuclear division). Chromosomes are not clearly d ...
... interphase is technically not part of mitosis, but rather encompasses stages G1, S, and G2 of the cell cycle. Interphase The cell is engaged in metabolic activity and performing its prepare for mitosis (the next four phases that lead up to and include nuclear division). Chromosomes are not clearly d ...
The roles of microtubules in tropisms
... elongating and swell by expanding radially (see refs. [47–53] for examples). In addition, there are several mutations that alter cortical microtubule organization and plants with these mutations have defects in the control of directional cell elongation. For example, microtubules become very short a ...
... elongating and swell by expanding radially (see refs. [47–53] for examples). In addition, there are several mutations that alter cortical microtubule organization and plants with these mutations have defects in the control of directional cell elongation. For example, microtubules become very short a ...
Microtubule
Microtubules (micro- + tube + -ule) are a component of the cytoskeleton, found throughout the cytoplasm. These tubular polymers of tubulin can grow as long as 50 micrometres and are highly dynamic. The outer diameter of a microtubule is about 24 nm while the inner diameter is about 12 nm. They are found in eukaryotic cells and are formed by the polymerization of a dimer of two globular proteins, alpha and beta tubulin.Microtubules are very important in a number of cellular processes. They are involved in maintaining the structure of the cell and, together with microfilaments and intermediate filaments, they form the cytoskeleton. They also make up the internal structure of cilia and flagella.They provide platforms for intracellular transport and are involved in a variety of cellular processes, including the movement of secretory vesicles, organelles, and intracellular macromolecular assemblies (see entries for dynein and kinesin). They are also involved in chromosome separation (mitosis and meiosis), and are the major constituents of mitotic spindles, which are used to pull apart eukaryotic chromosomes.Microtubules are nucleated and organized by microtubule organizing centers (MTOCs), such as the centrosome found in the center of many animal cells or the basal bodies found in cilia and flagella, or the spindle pole bodies found in fungi.There are many proteins that bind to microtubules, including the motor proteins kinesin and dynein, severing proteins like katanin, and other proteins important for regulating microtubule dynamics.