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Genes involved in asexual sporophyte development in Ceratopteris
Genes involved in asexual sporophyte development in Ceratopteris

... genes that increase in transcription in gametophytes during this commitment time. By comparing the Gene Ontology terms mapped to this cDNA library with that of the gametophyte transcriptome of the homosporous fern Pteridium aquilinum, I discovered that the C. richardii apogamy library is enriched in ...
View Tutorial
View Tutorial

... The mechanism of translation, by which proteins are produced in the cell, is one of the oldest and most fundamental cellular processes. Coding regions of DNA are transcribed to mRNA molecules, which will ultimately be translated into protein by the ribosome. Proteins are composed of linear chains of ...
Collaborative coupling between polymerase and helicase for
Collaborative coupling between polymerase and helicase for

... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
MTHFR C677T and A1298C: Explained In Plain
MTHFR C677T and A1298C: Explained In Plain

... Heterozygous A1298C is thought to be of minor consequence (if any), while heterozygous C677T may affect folate metabolism by up to 35% (3, 11). MTHFR mutations that have the greatest influence on health are: Homozygous A1298C (C1298C) and homozygous C677T (T677T) Compound heterozgous A1298C + C677T ...
Antibiotic susceptibility pattern among Staphylococcus spp. with
Antibiotic susceptibility pattern among Staphylococcus spp. with

... means isolates with MIC≥512µg/ml harbor a high copy number of mecA genes and regulatory elements (4,17). For achieving this hypothesis, all PCR tests were performed using same amount of template DNA and with similar concentration. It may also be due to the availability of sites of DNA amplification. ...
Endoplasmic Reticulum Protein Quality Control Failure in Myelin
Endoplasmic Reticulum Protein Quality Control Failure in Myelin

... biogenesis, Schwann cells and oligodendrocytes are committed to preserve long-term myelin stability and functionality. Defective myelin development (dysmyelination) or myelin instability and its consequent degeneration (demyelination) often result in mild-to-very severe neurological and neuromuscula ...
Androgen insensitivity syndrome
Androgen insensitivity syndrome

... Similarly, although AIS individuals may report any gender identity, a female gender identity is the gender identity of most, but not all, genetically-male female-bodied individuals with CAIS. Historically, however, the gender identity of CAIS individuals who identify as female has often been the cau ...
Tracing the Thread of Plastid Diversity through the Tapestry of Life
Tracing the Thread of Plastid Diversity through the Tapestry of Life

... the plastid genome are encoded in the nuclear genome and must be targeted to the plastid. Three mechanisms underlie this reduction of the plastid genome; gene loss, substitution, and transfer. First, in the case of gene loss, genes that no longer confer a selective advantage in the endosymbiotic env ...
DNA Genetics
DNA Genetics

... b. anticodon on the mRNA only. c. anticodon on the tRNA to which the amino acid is attached only. d. codon on the mRNA and the anticodon on the tRNA to which the amino acid is attached. ____ 23. Genes contain instructions for assembling a. purines. b. nucleosomes. ...
SPT3 interacts with TFIID to allow normal transcription in
SPT3 interacts with TFIID to allow normal transcription in

... purifies as a single polypeptide with the same molecular mass as predicted by the sequence of the yeast TFIIDencoding gene (Buratowski et al. 1988; Cavallini et al. 1989; Horikoshi et al. 1989). This result suggests either that yeast TFIID, unlike TFIID from higher eukaryotes, does not associate wit ...
GSEA - Bioinformatics Unit
GSEA - Bioinformatics Unit

... use HUGO gene symbols to identify the genes in the gene sets. 2. Choose false to use your expression dataset "as is." In this case, you are using the probe identifiers that are in your expression dataset for the analysis. The gene sets that you use for the analysis must also use these probe identifi ...
Oliver, B., Kim, Y.-J., and Baker, B. S.
Oliver, B., Kim, Y.-J., and Baker, B. S.

... cells and to order the functioning of other genes involved in germ-line sex determination relative to Sxl+. We first examined whether the sex specificity of Sxl+ function in the germ line was associated with sex-specific patterns of pre-mRNA splicing identical to those used to control Sxl+ activity ...
Foundations of Biology - Geoscience Research Institute
Foundations of Biology - Geoscience Research Institute

... XXY - Klinefelter’s Syndrome - Phenotypically male Of the non-sex chromosome aneuploids, Down’s Syndrome, extra chromosome 21, tends to be the most viable Down’s Syndrome is more common in children of mothers who gave birth after age 40 ©2000 Timothy G. Standish ...
Homeotic genes regulate the spatial expression
Homeotic genes regulate the spatial expression

... transcription patterns of target or 'realizator' genes (Garcia-Bellido, 1977). In the epidermis, the nervous system, and the visceral mesoderm, cross-regulatory interactions have been observed among the homeotic genes; thus the first target genes to have been identified are homeotic genes (Hafen et ...
A High Density Integrated Genetic Linkage Map of Soybean and the
A High Density Integrated Genetic Linkage Map of Soybean and the

... be the most abundant source of DNA polymorphisms in soybean (Hyten et al., 2006; Zhu et al., 2003). Despite being the most common molecular marker in soybean, the SNP frequency is relatively low compared to other cultivated crop species (Hyten et al., 2006; Zhu et al., 2003). The relatively low sequ ...
D:\My Documents\WordPerfect\WordPerfect 8.0\2001Report
D:\My Documents\WordPerfect\WordPerfect 8.0\2001Report

... (intron) sequences. Each individual gene differs not only with respect to its DNA sequence but also with respect to its structure. A few human genes, e.g. histone genes, interferon genes, and mitochondrial genes, are devoid of introns, whereas some possess a considerable number of introns, with thei ...
Identification and isolation of active N2O reducers in rice paddy soil
Identification and isolation of active N2O reducers in rice paddy soil

... Evaluation of the soil microcosm. Based on the preliminary experiments, all of the added N2O disappeared within 24 h of incubation when <2% N2O was added (data not shown). Since N2O should always be present to minimize utilization of succinate by metal reducers, the concentration of N2O should be >2 ...
SPATULA, a bHLH carpel development gene
SPATULA, a bHLH carpel development gene

... and pollen tract tissues throughout their development confirming its role in regulating their growth. It is also expressed in many other tissues where it may act redundantly to control growth, including the peripheral zone of the shoot apical meristem, and specific tissues within leaves, petals, sta ...
SERIES ‘‘GENETICS OF ASTHMA AND COPD IN THE POSTGENOME ERA’’
SERIES ‘‘GENETICS OF ASTHMA AND COPD IN THE POSTGENOME ERA’’

Multilocus genetic models of handedness closely resemble
Multilocus genetic models of handedness closely resemble

... are well described by single-locus Mendelian models. Here we summarize a large genome-wide association study (GWAS) that finds no significant associations with handedness and is consistent with a meta-analysis of GWASs. The GWAS had 99% power to detect a single locus using the conventional criterion ...
What role does natural selection play in speciation?
What role does natural selection play in speciation?

... between ancestral and derived alleles in the same lineage; the root could lie anywhere on the path connecting the present-day populations; figure 1.) Orr & Turelli (2001) assume that there is a highly skewed distribution of fitness effects, with a very small fraction of allelic combinations showing ...
Regulation of Na/K-ATPase β1 -subunit gene
Regulation of Na/K-ATPase β1 -subunit gene

... incubation at 37°C in humidified air with 5% CO2, medium was changed to one with the same composition as above, but without serum. All experiments were done after 48 h of further incubation under serum-free conditions. These cultures contain more than 95% myocytes as assessed by immunofluorescence s ...
Role of the p21 Protein in the Fanconi Anemia Pathway
Role of the p21 Protein in the Fanconi Anemia Pathway

... • Treat HCT116 p21+/+ and HCT116 p21-/- cells with the DNAcrosslinking agent mitomycin C (MMC). • Allow cells to grow for about 10 days. • Stain with crystal violet to quantify cells that survived. • Observe whether cells lacking p21 (HCT116 p21-/-) are more sensitive to MMC than wild type cells (HC ...
Clin Infect Dis. - Repositorio Académico UPC
Clin Infect Dis. - Repositorio Académico UPC

... The interpretation of the isolation rate of a pathogen in stool samples from children with diarrhea, compared with asymptomatic colonization, is complex. Factors that should be considered in interpretation include bacterial factors (bacteria with more virulent genes), host factors (age, genetic susc ...
LEADING ARTICLE Concurrent disruption of p16INK4a and
LEADING ARTICLE Concurrent disruption of p16INK4a and

... an important link between p53 and the p16INK4a-Rb pathway.29,30 Expression of ARF, which is induced by hyperproliferative stimuli like the Myc and E1A oncogenes31,32 but not by DNA damage,33,34 results in apoptosis or cell growth arrest in both G1 and G2.33,35 ARF and p16INK4a are encoded by a singl ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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