Analysis of large and small colony L5178Y tk−/− mouse lymphoma
... 20 s and 72°C extension for 20 s. The annealing temperature was decreased by 1°C for nine additional two-cycle sets until two cycles were performed at an annealing temperature of 52°C. Then 12 additional cycles were performed using an annealing temperature of 50°C, followed by a 72°C extension for 5 ...
... 20 s and 72°C extension for 20 s. The annealing temperature was decreased by 1°C for nine additional two-cycle sets until two cycles were performed at an annealing temperature of 52°C. Then 12 additional cycles were performed using an annealing temperature of 50°C, followed by a 72°C extension for 5 ...
Multiplex PCR for specific identification and determination of mating
... isolates from individual diseased vines on autoclaved cane sections (Mostert et al., 2003). Formation of perithecia for T. minima on dead vascular tissues and surface of decayed pruning wounds under field conditions have been reported by Rooney-Latham et al. (2005b). The presence of perithecia in vi ...
... isolates from individual diseased vines on autoclaved cane sections (Mostert et al., 2003). Formation of perithecia for T. minima on dead vascular tissues and surface of decayed pruning wounds under field conditions have been reported by Rooney-Latham et al. (2005b). The presence of perithecia in vi ...
Genetics of Clubroot Resistance inBrassicaSpecies | SpringerLink
... details of CR QTLs and their number and location in specific chromosomes in each species are discussed here. Brassica rapa Genetic analysis and genetic mapping of CR genes are well studied in B. rapa. All eight possible CR genes present in B. rapa have been identified through QTL mapping by research ...
... details of CR QTLs and their number and location in specific chromosomes in each species are discussed here. Brassica rapa Genetic analysis and genetic mapping of CR genes are well studied in B. rapa. All eight possible CR genes present in B. rapa have been identified through QTL mapping by research ...
Laboratory testing of CYP2D6 alleles in relation to tamoxifen therapy
... this may be a limitation of particular platforms. For example, the gene-converted *36 nonfunctional allele may mistakenly be called a *10 decreased-function allele.5 Critical to phenotype interpretation is a good understanding of the functional consequences of a given variant as regards enzyme activ ...
... this may be a limitation of particular platforms. For example, the gene-converted *36 nonfunctional allele may mistakenly be called a *10 decreased-function allele.5 Critical to phenotype interpretation is a good understanding of the functional consequences of a given variant as regards enzyme activ ...
Tutorial - QIAGEN Bioinformatics
... methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. Changes in cytosine methylation levels are implicated in regulation of gene expression, and have been shown to persist over generations, thus providing mechanistic basis for epigenetic inheritance. T ...
... methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. Changes in cytosine methylation levels are implicated in regulation of gene expression, and have been shown to persist over generations, thus providing mechanistic basis for epigenetic inheritance. T ...
American College of Medical Genetics standards
... not imply clinical significance; therefore, a qualifier such a pathogenic CNV or benign CNV is necessary for clear communication of clinical relevance. In addition, the term “CNV” does not imply relative dosage. Copy number loss (deletion) or copy number gain (duplication) must be specified to clari ...
... not imply clinical significance; therefore, a qualifier such a pathogenic CNV or benign CNV is necessary for clear communication of clinical relevance. In addition, the term “CNV” does not imply relative dosage. Copy number loss (deletion) or copy number gain (duplication) must be specified to clari ...
An SMN-Dependent U12 Splicing Event Essential for Motor
... effects of SMN deficiency on snRNP biology in vivo. Spliceosomal snRNPs comprise two distinct classes, each dedicated to the removal of different intron types. Most eukaryotic introns are processed by the major (U2-dependent) spliceosome formed by U1, U2, U4/U6, and U5 snRNPs, whereas a small propo ...
... effects of SMN deficiency on snRNP biology in vivo. Spliceosomal snRNPs comprise two distinct classes, each dedicated to the removal of different intron types. Most eukaryotic introns are processed by the major (U2-dependent) spliceosome formed by U1, U2, U4/U6, and U5 snRNPs, whereas a small propo ...
American College of Medical Genetics standards and guidelines for
... not imply clinical significance; therefore, a qualifier such a pathogenic CNV or benign CNV is necessary for clear communication of clinical relevance. In addition, the term “CNV” does not imply relative dosage. Copy number loss (deletion) or copy number gain (duplication) must be specified to clari ...
... not imply clinical significance; therefore, a qualifier such a pathogenic CNV or benign CNV is necessary for clear communication of clinical relevance. In addition, the term “CNV” does not imply relative dosage. Copy number loss (deletion) or copy number gain (duplication) must be specified to clari ...
A rule-based kinetic model of RNA polymerase II C
... modelled as acting on Ser2P during elongation and on both Ser2P and Ser5P during recycling. The Ser2 kinase Bur1 is recruited to CTD repeats of elongating RNAPII phosphorylated on Ser5. Bur1 augments the major Ser2 kinase (Ctk1) and is capable of phosphorylating CTD repeats at Ser2, when Ctk1 is ...
... modelled as acting on Ser2P during elongation and on both Ser2P and Ser5P during recycling. The Ser2 kinase Bur1 is recruited to CTD repeats of elongating RNAPII phosphorylated on Ser5. Bur1 augments the major Ser2 kinase (Ctk1) and is capable of phosphorylating CTD repeats at Ser2, when Ctk1 is ...
A Genome-Wide Survey of the NAC Transcription
... acid residues and is divided into five sub-domains (Ooka et al., 2003). The function of the NAC domain has been associated with nuclear localization, DNA binding, and the formation of homodimers or heterodimers with other proteins that contain NAC domains (Olsen et al., 2005). The C-terminal regions ...
... acid residues and is divided into five sub-domains (Ooka et al., 2003). The function of the NAC domain has been associated with nuclear localization, DNA binding, and the formation of homodimers or heterodimers with other proteins that contain NAC domains (Olsen et al., 2005). The C-terminal regions ...
The hidden complexity of Mendelian traits across yeast
... Hidden complexity of a rare Mendelian variant across different genetic backgrounds While focusing on highly frequent cases such as CuSO4 and NaCl provided indications about the transmission stability of common Mendelian variants and revealed previously unknown co-segregations, we were particularly i ...
... Hidden complexity of a rare Mendelian variant across different genetic backgrounds While focusing on highly frequent cases such as CuSO4 and NaCl provided indications about the transmission stability of common Mendelian variants and revealed previously unknown co-segregations, we were particularly i ...
the lkb1 tumor suppressor - E
... Gatekeepers and Caretakers Tumor suppressors can be further divided into gatekeepers and caretakers based on gene function (Kinzler and Vogelstein, 1997). Gatekeepers prevent neoplasia directly by controlling cell growth, either by regulating proliferation or by promoting cell death. Although multip ...
... Gatekeepers and Caretakers Tumor suppressors can be further divided into gatekeepers and caretakers based on gene function (Kinzler and Vogelstein, 1997). Gatekeepers prevent neoplasia directly by controlling cell growth, either by regulating proliferation or by promoting cell death. Although multip ...
Control of the meiotic cell division program in plants | SpringerLink
... during mitosis and meiosis. a Progression through mitosis is thought to rely on increasing levels of CDK activity (black line). Medium levels of CDK activity are required for the induction of S phase, and high levels are necessary to promote M phase. Putative threshold levels for S phase are indicat ...
... during mitosis and meiosis. a Progression through mitosis is thought to rely on increasing levels of CDK activity (black line). Medium levels of CDK activity are required for the induction of S phase, and high levels are necessary to promote M phase. Putative threshold levels for S phase are indicat ...
Saccharomyces cerevisiae
... for deficiencies due to the differing glycosylation and improve secretion of the functional enzyme in this heterologous host. Through three generations of random mutagenesis and screening for activity, we have increased the total active, secreted HRP to levels where it is now feasible to tailor this ...
... for deficiencies due to the differing glycosylation and improve secretion of the functional enzyme in this heterologous host. Through three generations of random mutagenesis and screening for activity, we have increased the total active, secreted HRP to levels where it is now feasible to tailor this ...
Suggestive Association With Ocular Phoria at Chromosome 6p22
... The minor allele frequency of the lead SNP rs1569579 was 9% in our sample. Each additional copy of the C allele was associated with a shift in phoria of 0.45 SDs in the direction of esophoria. Variation at rs1569579 explains 3.2% of the total variance in near horizontal phoria. Figure 5 shows distri ...
... The minor allele frequency of the lead SNP rs1569579 was 9% in our sample. Each additional copy of the C allele was associated with a shift in phoria of 0.45 SDs in the direction of esophoria. Variation at rs1569579 explains 3.2% of the total variance in near horizontal phoria. Figure 5 shows distri ...
PDF
... of tilt' W'llC pairs. illld that c\omini1l1t g('lleS lind !L gl'cllter effect in tlU',;C' gt' I1otypt's thiUl they did in genotypes hn\'ing nt least one gene pill I' 1'('('(Is;~,in'. Frolll titblt' :2 it ('lInbe S('C'Il tIutt 21.5 pcrC'ent of the plnnts; of Porter and 9,1 IWrCt'nL or tht, plants of ...
... of tilt' W'llC pairs. illld that c\omini1l1t g('lleS lind !L gl'cllter effect in tlU',;C' gt' I1otypt's thiUl they did in genotypes hn\'ing nt least one gene pill I' 1'('('(Is;~,in'. Frolll titblt' :2 it ('lInbe S('C'Il tIutt 21.5 pcrC'ent of the plnnts; of Porter and 9,1 IWrCt'nL or tht, plants of ...
The molecular basis of human retinal and vitreoretinal
... (p.Ala295Val) in rhodopsin was found in a Swiss family with autosomal dominant CSNB. It was characterized in a functional assay, where the catalytic GTP exchange of transducin was measured. In the presence of 11-cis retinal, the respective mutant rhodopsin was found to be inactive, similar to wild-t ...
... (p.Ala295Val) in rhodopsin was found in a Swiss family with autosomal dominant CSNB. It was characterized in a functional assay, where the catalytic GTP exchange of transducin was measured. In the presence of 11-cis retinal, the respective mutant rhodopsin was found to be inactive, similar to wild-t ...
factor involved in dorsal-ventral axis formation and neurogenesis
... that fragment. MNR31, MNR62, cl-7, and MNR73 all contain exon 5 from the 1.3-kb EcoRI fragment and, thus, differ only in the 5'-most exon. MNR22 begins at the same site as MNR73 but reads through the splice signals utilized by MNR73. The final cDNA examined, c3-0, appears to be a partial unprocessed ...
... that fragment. MNR31, MNR62, cl-7, and MNR73 all contain exon 5 from the 1.3-kb EcoRI fragment and, thus, differ only in the 5'-most exon. MNR22 begins at the same site as MNR73 but reads through the splice signals utilized by MNR73. The final cDNA examined, c3-0, appears to be a partial unprocessed ...
Lanosterol Biosynthesis in the Prokaryote
... Recently, we have isolated and characterized the M. capsulatus lanosterol 14a-demethylase (MCCYP51FX) (other names CYP51, P45014DM) (Jackson et al. 2002). This protein was, surprisingly, a soluble form, unlike the membrane-bound eukaryotic counterparts (Lamb et al. 1999), and represented a new class ...
... Recently, we have isolated and characterized the M. capsulatus lanosterol 14a-demethylase (MCCYP51FX) (other names CYP51, P45014DM) (Jackson et al. 2002). This protein was, surprisingly, a soluble form, unlike the membrane-bound eukaryotic counterparts (Lamb et al. 1999), and represented a new class ...
Persistence and Loss of Meiotic Recombination
... and degraded at the initiation site in only one of the interacting homologs—and this asymmetry is the source of the paradox. The asymmetry of the DSBR model was necessitated by the asymmetric gene conversion seen in crosses between strains of ascomycete fungi containing more-active and less-active a ...
... and degraded at the initiation site in only one of the interacting homologs—and this asymmetry is the source of the paradox. The asymmetry of the DSBR model was necessitated by the asymmetric gene conversion seen in crosses between strains of ascomycete fungi containing more-active and less-active a ...
Lecture 3: Sequence Alignment
... The Smith Waterman algorithm The Smith-Waterman algorithm (1981) is for determining similar regions between two nucleotide or protein sequences. Smith-Waterman is also a dynamic programming algorithm and improves on Needleman-Wunsch. As such, it has the desirable property that it is guaranteed to f ...
... The Smith Waterman algorithm The Smith-Waterman algorithm (1981) is for determining similar regions between two nucleotide or protein sequences. Smith-Waterman is also a dynamic programming algorithm and improves on Needleman-Wunsch. As such, it has the desirable property that it is guaranteed to f ...
Ch. 14 Mendel and the Gene Idea
... based on enzymes that adds a carbohydrate to a red blood cell ...
... based on enzymes that adds a carbohydrate to a red blood cell ...
Mitochondrial diseases and the role of the yeast models
... Yeast (Saccharomyces cerevisiae) is a simple eukaryotic organism, with a complete genome sequence and, more importantly, the best annotated one, thanks to a coordinated international effort. Many genetic tools that have been created during these years, including the complete collection of gene delet ...
... Yeast (Saccharomyces cerevisiae) is a simple eukaryotic organism, with a complete genome sequence and, more importantly, the best annotated one, thanks to a coordinated international effort. Many genetic tools that have been created during these years, including the complete collection of gene delet ...
PDF
... The spatiotemporal dynamics of oscillations create a phase difference among cells along the PSM. Anterior PSM cells are retarded in the oscillator phase relative to posterior PSM cells; the further anterior the cells are located, the greater the retardation is. This phase difference results in the f ...
... The spatiotemporal dynamics of oscillations create a phase difference among cells along the PSM. Anterior PSM cells are retarded in the oscillator phase relative to posterior PSM cells; the further anterior the cells are located, the greater the retardation is. This phase difference results in the f ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse