coloured foal booklet - British Skewbald and Piebald Association
... As each parent gives its foal ONE of those genes, it takes two coloured parents to produce a homozygous (TT) horse. Hence a horse with one known solid parent can never be homozygous for the Tobiano gene and therefore will only produce 50% of coloured offspring to solid mares. A solid foal by two Tob ...
... As each parent gives its foal ONE of those genes, it takes two coloured parents to produce a homozygous (TT) horse. Hence a horse with one known solid parent can never be homozygous for the Tobiano gene and therefore will only produce 50% of coloured offspring to solid mares. A solid foal by two Tob ...
Bioinformatics
... • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with ...
... • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with ...
Two Waves of Nuclear Factor κB Recruitment to Target Promoters
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
Pet_SUMO manual
... protein (Saitoh et al., 1997). Smt3, hereby referred to as SUMO, is a member of a ubiquitin-like protein family that regulates several cellular processes including apoptosis, nuclear transport, and cell cycle progression (Muller et al., 2001). Like ubiquitin, SUMO covalently attaches to lysine side ...
... protein (Saitoh et al., 1997). Smt3, hereby referred to as SUMO, is a member of a ubiquitin-like protein family that regulates several cellular processes including apoptosis, nuclear transport, and cell cycle progression (Muller et al., 2001). Like ubiquitin, SUMO covalently attaches to lysine side ...
Gbx2 is required for the morphogenesis of the mouse inner ear
... with an enlarged membranous labyrinth (Fig. 2A). By contrast, the lateral canal and ampulla are usually present (Table I, n=17/19). Type I, the mildest phenotype, shows an enlarged membranous labyrinth, and three out of the four specimens are missing the endolymphatic duct (Table 1). In the Type II ...
... with an enlarged membranous labyrinth (Fig. 2A). By contrast, the lateral canal and ampulla are usually present (Table I, n=17/19). Type I, the mildest phenotype, shows an enlarged membranous labyrinth, and three out of the four specimens are missing the endolymphatic duct (Table 1). In the Type II ...
Gene Name
... glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes. Same as A ...
... glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes. Same as A ...
Variable Expression of the Mutation in Familial Defective
... inherited a rare mutant non-FDB allele at the apoB locus, whose product was underrepresented in his plasma. In support of this, they showed that the IQo of J.H.'s LDL (determined by a competitive binding assay) was two to three times the IQoS of the LDLs obtained from his heterozygous relatives, and ...
... inherited a rare mutant non-FDB allele at the apoB locus, whose product was underrepresented in his plasma. In support of this, they showed that the IQo of J.H.'s LDL (determined by a competitive binding assay) was two to three times the IQoS of the LDLs obtained from his heterozygous relatives, and ...
Valpromide Inhibits Lytic Cycle Reactivation of Epstein
... previously that butyrate upregulates expression of a luciferase (luc) reporter driven by the BZLF1 promoter (Zp) and that VPA blocks Zp-luc expression by butyrate. Here, we show that VPM, like VPA, does not induce Zp-luc expression and blocks stimulation of this reporter by butyrate (Fig. 5). We tes ...
... previously that butyrate upregulates expression of a luciferase (luc) reporter driven by the BZLF1 promoter (Zp) and that VPA blocks Zp-luc expression by butyrate. Here, we show that VPM, like VPA, does not induce Zp-luc expression and blocks stimulation of this reporter by butyrate (Fig. 5). We tes ...
CONSERVATION AND DIVERGENCE IN MOLECULAR
... DV Axiation Processes in D. melanogaster Our understanding of DV patterning during early embryogenesis in D. melanogaster is based on the genetic dissection of the Toll/dorsal pathway (Figure 2) (reviewed in 100). Upon mutation, 12 genes show maternal-effect dorsoventral patterning defects in the em ...
... DV Axiation Processes in D. melanogaster Our understanding of DV patterning during early embryogenesis in D. melanogaster is based on the genetic dissection of the Toll/dorsal pathway (Figure 2) (reviewed in 100). Upon mutation, 12 genes show maternal-effect dorsoventral patterning defects in the em ...
Article interaction G x tabac - Hal-CEA
... As mentioned in the method section, we also considered the passive ETS in-utero and/or in early childhood phenotype, in addition to passive ETS in early childhood. But this led to unchanged results. Indeed, most of the mothers which smoked during pregnancy also continued to smoke during the early-c ...
... As mentioned in the method section, we also considered the passive ETS in-utero and/or in early childhood phenotype, in addition to passive ETS in early childhood. But this led to unchanged results. Indeed, most of the mothers which smoked during pregnancy also continued to smoke during the early-c ...
Effect of the allelic variants of aldehyde
... The liver is the major organ for ethanol metabolism.1,16,32 Acetaldehyde concentrations in hepatocytes depend on both the rate of generation (ie ethanol oxidation by ADHs) and the rate of removal (ie acetaldehyde oxidation by ALDHs). The functional polymorphic variants of ADH/ALDH genes can alter th ...
... The liver is the major organ for ethanol metabolism.1,16,32 Acetaldehyde concentrations in hepatocytes depend on both the rate of generation (ie ethanol oxidation by ADHs) and the rate of removal (ie acetaldehyde oxidation by ALDHs). The functional polymorphic variants of ADH/ALDH genes can alter th ...
The Structures of DNA and RNA
... structures. These structures are full of surprises, such as non-classical base pairs, base-backbone interactions, and knot-like configurations. Most remarkable of all, and of profound evolutionary significance, some RNA molecules are enzymes that carry out reactions that are at the core of informati ...
... structures. These structures are full of surprises, such as non-classical base pairs, base-backbone interactions, and knot-like configurations. Most remarkable of all, and of profound evolutionary significance, some RNA molecules are enzymes that carry out reactions that are at the core of informati ...
Genomic dissection of plant development and its
... shooting lines also tend to be early for other stages. Another peculiarity is that in the case of RIP correlation coefficients were negative for all other developmental stages. Together with the relatively low coefficient of variation for MAT, this indicates that the time of maturity may be predeter ...
... shooting lines also tend to be early for other stages. Another peculiarity is that in the case of RIP correlation coefficients were negative for all other developmental stages. Together with the relatively low coefficient of variation for MAT, this indicates that the time of maturity may be predeter ...
Electrophoresis Revised
... placed firmly on the end. Since the principle by which the micropipette works is the creation of a vacuum in the tip, causing liquid to be drawn up, it is critical that the tip be on tight enough to make an air-tight seal. Having said this, do NOT jam the tips on so hard that they are hard to get of ...
... placed firmly on the end. Since the principle by which the micropipette works is the creation of a vacuum in the tip, causing liquid to be drawn up, it is critical that the tip be on tight enough to make an air-tight seal. Having said this, do NOT jam the tips on so hard that they are hard to get of ...
CHAPTER 7 TUNING THE DRAGON PROMOTER FINDER SYSTEM FOR HUMAN PROMOTER RECOGNITION
... diverse transcription factors that individually bind to specific DNA consensus sequences—called transcription factor binding sites—in the promoter to activate the transcriptional machinery in concert with RNA polymerases. Many simplistic approaches have been taken in computational promoter recogniti ...
... diverse transcription factors that individually bind to specific DNA consensus sequences—called transcription factor binding sites—in the promoter to activate the transcriptional machinery in concert with RNA polymerases. Many simplistic approaches have been taken in computational promoter recogniti ...
user guide here
... Contiguous non-variant segments of the genome can be represented as single records in gVCF. These records use the standard 'END' INFO key to indicate the extent of the record. Even though the record can span multiple bases, only the first base is provided in the REF field to reduce file size. The fo ...
... Contiguous non-variant segments of the genome can be represented as single records in gVCF. These records use the standard 'END' INFO key to indicate the extent of the record. Even though the record can span multiple bases, only the first base is provided in the REF field to reduce file size. The fo ...
A Genetic-Based Evaluation of the Principal Tissue Reservoir for
... (RAPD). Chromosomal DNA was purified and PCR amplifications were done as described above, with the following modification: A single 10-mer oligonucleotide primer (p17; 5*-GATCTGACAC-3*) was used at a concentration of 2.0 mM and MgCl2 was included at 2.5 mM [22]. Thermal cycling was performed as desc ...
... (RAPD). Chromosomal DNA was purified and PCR amplifications were done as described above, with the following modification: A single 10-mer oligonucleotide primer (p17; 5*-GATCTGACAC-3*) was used at a concentration of 2.0 mM and MgCl2 was included at 2.5 mM [22]. Thermal cycling was performed as desc ...
Understanding the basis of auriculocondylar syndrome: Insights
... PLCD4 produces a naturally occurring splice variant coding for an isoform that can dominantly interfere with various PLC proteins [Nagano et al., 1999]. An informative in vivo test of this dominant negative theory will require overexpression in zebrafish embryos of wild type human PLCB4/GNAI3 or ver ...
... PLCD4 produces a naturally occurring splice variant coding for an isoform that can dominantly interfere with various PLC proteins [Nagano et al., 1999]. An informative in vivo test of this dominant negative theory will require overexpression in zebrafish embryos of wild type human PLCB4/GNAI3 or ver ...
A Comparative Study on the Recovery of EcoRI Endonuclease from
... Growth of Cells: The culture medium used was LB medium containing 1% (w/v) bacto-tryptone (Difco), 0.5% (w/v) yeast extract (Difco) and 1% (w/v) NaCl. E. coli M5248 was cultured in LB medium supplemented with 0.01M K-phosphate (pH 7.0), 0.01% (w/v) thymine, 0.005% (w/v) thiamine and 5% (w/v) glucose ...
... Growth of Cells: The culture medium used was LB medium containing 1% (w/v) bacto-tryptone (Difco), 0.5% (w/v) yeast extract (Difco) and 1% (w/v) NaCl. E. coli M5248 was cultured in LB medium supplemented with 0.01M K-phosphate (pH 7.0), 0.01% (w/v) thymine, 0.005% (w/v) thiamine and 5% (w/v) glucose ...
Genes involved in asexual sporophyte development in Ceratopteris
... genes that increase in transcription in gametophytes during this commitment time. By comparing the Gene Ontology terms mapped to this cDNA library with that of the gametophyte transcriptome of the homosporous fern Pteridium aquilinum, I discovered that the C. richardii apogamy library is enriched in ...
... genes that increase in transcription in gametophytes during this commitment time. By comparing the Gene Ontology terms mapped to this cDNA library with that of the gametophyte transcriptome of the homosporous fern Pteridium aquilinum, I discovered that the C. richardii apogamy library is enriched in ...
Why are most organelle genomes transmitted maternally?
... (sorting-out) of genetically distinct organelles (Box 1; Fig. 1), and the virtual absence of recombination [1, 2]. Due to the different evolutionary origins and inheritance modes of the genomes of the eukaryotic cell, severe evolutionary consequences arise: (i) Nuclear and organellar genomes differ ...
... (sorting-out) of genetically distinct organelles (Box 1; Fig. 1), and the virtual absence of recombination [1, 2]. Due to the different evolutionary origins and inheritance modes of the genomes of the eukaryotic cell, severe evolutionary consequences arise: (i) Nuclear and organellar genomes differ ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse