A component of calcium-activated potassium channels encoded by
... restriction fiagrnent within this cosmid. In slo4, the 3-kb Eco RI genomic fragment at 96F5-8 is broken into two parts, each juxtaposed to DNA sequences from the slo locus at 96A17. A phage containing one of these junction fiagments was isolated by probing a slo4 genomic library with the 3-kb wild-t ...
... restriction fiagrnent within this cosmid. In slo4, the 3-kb Eco RI genomic fragment at 96F5-8 is broken into two parts, each juxtaposed to DNA sequences from the slo locus at 96A17. A phage containing one of these junction fiagments was isolated by probing a slo4 genomic library with the 3-kb wild-t ...
Mycoplasma genitalium: a brief review
... (816 kb), it has been shown that M. genitalium contains some subsets of M. pneumoniae’s genomic complement and that the coding genes in the M. genitalium genome correspond to certain sequences of M. pneumoniae’s genome.14 The small genome of M. genitalium gives a good indication of the minimal set o ...
... (816 kb), it has been shown that M. genitalium contains some subsets of M. pneumoniae’s genomic complement and that the coding genes in the M. genitalium genome correspond to certain sequences of M. pneumoniae’s genome.14 The small genome of M. genitalium gives a good indication of the minimal set o ...
Biology 30 January 2000 Diploma Examination and Key
... increasing levels of estrogen in pregnant sheep decreasing production of cortisol by the fetal lamb increasing levels of progesterone in pregnant sheep decreasing activity of the hypothalamus by the fetal lamb ...
... increasing levels of estrogen in pregnant sheep decreasing production of cortisol by the fetal lamb increasing levels of progesterone in pregnant sheep decreasing activity of the hypothalamus by the fetal lamb ...
Regulation of meiotic progression by the meiosis
... synchronous meiosis in mek1 and wild-type cells at 15 minute time points (Fig. 4B). This analysis revealed that premeiotic DNA replication takes place with the same kinetics in both wild-type and mek1 cells. Therefore, since entry into meiosis I occurs earlier in mek1, this implies that meiotic prop ...
... synchronous meiosis in mek1 and wild-type cells at 15 minute time points (Fig. 4B). This analysis revealed that premeiotic DNA replication takes place with the same kinetics in both wild-type and mek1 cells. Therefore, since entry into meiosis I occurs earlier in mek1, this implies that meiotic prop ...
Role of two-component systems in the virulence of Streptococcus
... histidine residue. HK proteins tend to be found as homodimers that operate in trans with the kinase domain of one catalysing phosphorylation of the second (Dutta et al., 1999). This phosphate group is then transferred by the HK to a conserved aspartate residue in its cognate RR. In turn, the RR unde ...
... histidine residue. HK proteins tend to be found as homodimers that operate in trans with the kinase domain of one catalysing phosphorylation of the second (Dutta et al., 1999). This phosphate group is then transferred by the HK to a conserved aspartate residue in its cognate RR. In turn, the RR unde ...
lilliputian - Development - The Company of Biologists
... been proposed to play a direct role in regulating the dynamic reorganization and interaction of the contractile actincytoskeleton with the newly forming plasma membranes of cellularizing embryos (Schweisguth et al., 1990; SimpsonRose and Wieschaus, 1992; Schejter and Wieschaus, 1993b; Hunter and Wie ...
... been proposed to play a direct role in regulating the dynamic reorganization and interaction of the contractile actincytoskeleton with the newly forming plasma membranes of cellularizing embryos (Schweisguth et al., 1990; SimpsonRose and Wieschaus, 1992; Schejter and Wieschaus, 1993b; Hunter and Wie ...
A MIAME-compliant Microarray Database
... • In the first experiment, they found some genes regulated by addition of GA-4 • They made GFP fusions to three of these genes ...
... • In the first experiment, they found some genes regulated by addition of GA-4 • They made GFP fusions to three of these genes ...
Deciphering the Galaxy Guppy phenotype
... her farm in Granby, Massachusetts (Eisen 2005). Many of the strains she bred originated from Japan where mice had been domesticated for centuries. The guppy hobby has the same potential to provide contemporary researchers with color mutants that have been identified and isolated by hobbyists (Păsări ...
... her farm in Granby, Massachusetts (Eisen 2005). Many of the strains she bred originated from Japan where mice had been domesticated for centuries. The guppy hobby has the same potential to provide contemporary researchers with color mutants that have been identified and isolated by hobbyists (Păsări ...
Transcriptional Attenuation
... there are many examples of attenuation that differ in the way that termination is made conditional. We have divided them into four classes based on their common features. A single, well-studied example of each class is described in detail, and other examples are mentioned to illustrate unique points ...
... there are many examples of attenuation that differ in the way that termination is made conditional. We have divided them into four classes based on their common features. A single, well-studied example of each class is described in detail, and other examples are mentioned to illustrate unique points ...
SNPs for individual identification
... disequilibrium. In the case of the disease-causing allele in complete LD with one of the SNP alleles, while the SNP genotype does alter the numeric probability of the mutation being present, it is not a very meaningful alteration even in this extreme case of a relatively common disease-causing mutat ...
... disequilibrium. In the case of the disease-causing allele in complete LD with one of the SNP alleles, while the SNP genotype does alter the numeric probability of the mutation being present, it is not a very meaningful alteration even in this extreme case of a relatively common disease-causing mutat ...
Supplementary Information (doc 132K)
... Figure S5: Schematic of differential SNP missingness tests for cross-platform comparisons. 9961 SNPs were removed based on differential missingness with respect to phenotype (i.e. between cases and controls). An additional 4960 SNPs were removed due to differential missingness with respect to flanki ...
... Figure S5: Schematic of differential SNP missingness tests for cross-platform comparisons. 9961 SNPs were removed based on differential missingness with respect to phenotype (i.e. between cases and controls). An additional 4960 SNPs were removed due to differential missingness with respect to flanki ...
Variation in copper metabolism in Arabidopsis thaliana accessions
... chelation, efflux from cells, sequestration in subcellular compartments, and detoxification of ROS (Wintz and Vulpe 2002). To prevent damage induced by ROS, plants have evolved several detoxification mechanisms including the synthesis of enzymatic (e.g. superoxide dismutases) and nonenzymatic (e.g. ...
... chelation, efflux from cells, sequestration in subcellular compartments, and detoxification of ROS (Wintz and Vulpe 2002). To prevent damage induced by ROS, plants have evolved several detoxification mechanisms including the synthesis of enzymatic (e.g. superoxide dismutases) and nonenzymatic (e.g. ...
Alarmingly High Segregation Frequencies of Quinolone Resistance
... et al. 2009; Andersson and Hughes 2010; Kunz et al. 2012; Katz and Hershberg 2013; Miskinyte and Gordo 2013; Qi et al. 2014). Such strong fitness effects could greatly influence the frequencies with which TRAs segregate, in a manner that is independent of antibiotic exposure. The fitness effects of ...
... et al. 2009; Andersson and Hughes 2010; Kunz et al. 2012; Katz and Hershberg 2013; Miskinyte and Gordo 2013; Qi et al. 2014). Such strong fitness effects could greatly influence the frequencies with which TRAs segregate, in a manner that is independent of antibiotic exposure. The fitness effects of ...
From DNA to diversity: molecular genetics and the evolution of
... Animals diverge from common ancestors through changes in their DNA. The major question, then, is, Which changes in DNA account for morphological diversity? The answer to this question has eluded us for the half-century since the Modern Synthesis was proposed and the structure of DNA was discovered. ...
... Animals diverge from common ancestors through changes in their DNA. The major question, then, is, Which changes in DNA account for morphological diversity? The answer to this question has eluded us for the half-century since the Modern Synthesis was proposed and the structure of DNA was discovered. ...
PDF
... 2002) and is ultimately under the regulation of the neuropeptide prothoracicotropic hormone (PTTH) and a subsequent intracellular signaling pathway (Gilbert et al., 2002; Rewitz et al., 2009). A recent study reported that the Halloween gene spook (spo) and its paralog spookier (spok), which also enc ...
... 2002) and is ultimately under the regulation of the neuropeptide prothoracicotropic hormone (PTTH) and a subsequent intracellular signaling pathway (Gilbert et al., 2002; Rewitz et al., 2009). A recent study reported that the Halloween gene spook (spo) and its paralog spookier (spok), which also enc ...
Microbiology
... In several Pseudomonas species, the gacA and gacS genes are subject to spontaneous mutation, and gac mutants appear for example in nutrient-rich liquid medium (Bull et al., 2001; Duffy & Defago, 1995; van den Broek et al., 2003) and on plant roots (Sanchez-Contreras et al., 2002; Chancey et al., 200 ...
... In several Pseudomonas species, the gacA and gacS genes are subject to spontaneous mutation, and gac mutants appear for example in nutrient-rich liquid medium (Bull et al., 2001; Duffy & Defago, 1995; van den Broek et al., 2003) and on plant roots (Sanchez-Contreras et al., 2002; Chancey et al., 200 ...
View PDF - OSU Biochemistry and Molecular Biology
... indirect targets without additional and tedious experimentation. This knowledge, however, was regarded as essential, since direct targets could be used not only to explore Hox function, but also to elucidate the mechanisms of Hox target gene selection and regulation. In this context, chromatin immun ...
... indirect targets without additional and tedious experimentation. This knowledge, however, was regarded as essential, since direct targets could be used not only to explore Hox function, but also to elucidate the mechanisms of Hox target gene selection and regulation. In this context, chromatin immun ...
Biology Final Exam Review Questions Answer Section SHORT
... BLM: application 45. ANS: External forces (such as a drying pond) trigger internal factors (such as hormonal changes) that change the rate of metamorphosis. PTS: 1 DIF: L2 REF: p. 383 OBJ: 13.4.3 Relate gene regulation to development in multicellular organisms. STA: CA.BIO.4.d TOP: Foundation Editi ...
... BLM: application 45. ANS: External forces (such as a drying pond) trigger internal factors (such as hormonal changes) that change the rate of metamorphosis. PTS: 1 DIF: L2 REF: p. 383 OBJ: 13.4.3 Relate gene regulation to development in multicellular organisms. STA: CA.BIO.4.d TOP: Foundation Editi ...
Molecular characterization of glutathione peroxidase
... 207 bp and 738 bp upstream of the start codon respectively. Three homologous chicken CdxA caudal type homeobox protein binding sites and one octomer transcription factor 1 (OCT1) were also identified at 98 bp, 663 bp, 677 bp, 704 bp upstream of the start codon respectively. Furthermore, there were t ...
... 207 bp and 738 bp upstream of the start codon respectively. Three homologous chicken CdxA caudal type homeobox protein binding sites and one octomer transcription factor 1 (OCT1) were also identified at 98 bp, 663 bp, 677 bp, 704 bp upstream of the start codon respectively. Furthermore, there were t ...
The Frequency Distribution of Nucleotide Variation in Drosophila
... D. yakuba served as outgroups for the D. simulans data. I used a haphazardly selected allele from each of the two outgroup species for all inferences of the ancestral state in D. simulans. When each of the outgroup codons was identical to one of the segregating D. simulans codons, the outgroup codon ...
... D. yakuba served as outgroups for the D. simulans data. I used a haphazardly selected allele from each of the two outgroup species for all inferences of the ancestral state in D. simulans. When each of the outgroup codons was identical to one of the segregating D. simulans codons, the outgroup codon ...
Analysis of Genetic Toggle Switch Systems Encoded on Plasmids
... ing time , namely, the average time between spontaneous transitions. Two variants, the general switch and the exclusive switch, were studied and compared in Refs. [27]. The difference between these two variants is that in the exclusive switch there is an overlap between the two promoter sites, so t ...
... ing time , namely, the average time between spontaneous transitions. Two variants, the general switch and the exclusive switch, were studied and compared in Refs. [27]. The difference between these two variants is that in the exclusive switch there is an overlap between the two promoter sites, so t ...
Structure and function of the GINS complex, a key component of the
... and DDK substrates is key to understanding how replication initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 an ...
... and DDK substrates is key to understanding how replication initiation is regulated. S-CDK substrates are abundant in yeast but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 an ...
The new cardiac genetic testing panels
... The genetic test evaluation process (previously referred to as the Gene Dossier process) was developed by the UKGTN in 2003 as a tool to evaluate whether a proposed laboratory genetic test for a specific genetic disease is to be recommended for inclusion on the NHS Directory of Genetic Disorders/Gen ...
... The genetic test evaluation process (previously referred to as the Gene Dossier process) was developed by the UKGTN in 2003 as a tool to evaluate whether a proposed laboratory genetic test for a specific genetic disease is to be recommended for inclusion on the NHS Directory of Genetic Disorders/Gen ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse