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NanoString™: User Guide | nCounter® Expression Data Analysis
NanoString™: User Guide | nCounter® Expression Data Analysis

... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
Experimental procedures (detailed)
Experimental procedures (detailed)

... sequence similarities between ESTs of Plumbago zeylanica sperm cells and those of other gamete-related cells or organs, local tBLASTx searches were performed with a cut-off value of 1.0×e-10. ...
SARS Outbreaks in Ontario, Hong Kong and Singapore
SARS Outbreaks in Ontario, Hong Kong and Singapore

... (two biological advantages: maintaining chromosome number unchanged and crossing over between different genes) • Crossover: The interchange of sections between pairing homologous chromosomes during meiosis • Recombination, recombinant, recombination fraction (rate, frequency): The natural formation ...
Dr. Evan Fertig - Epilepsy Life Links
Dr. Evan Fertig - Epilepsy Life Links

... benefit from other types of genetic test:  Microscopic examination of the chromosomes  Metabolic tests- Blood tests to see how some ...
Understanding the pathological manifestations of aromatase excess
Understanding the pathological manifestations of aromatase excess

... Subchromosomal recombination events including duplication, deletion, and inversion has been identified. The latter two recombinations recruit novel promoters for CYP19A1 through a unique mechanism. Gynecomastia continues for life, and although the general condition is well preserved, it may cause ps ...
Cilia are at the heart of vertebrate left–right asymmetry
Cilia are at the heart of vertebrate left–right asymmetry

Gene Section member 2)) Atlas of Genetics and Cytogenetics
Gene Section member 2)) Atlas of Genetics and Cytogenetics

1 Sequential elimination of major-effect contributors
1 Sequential elimination of major-effect contributors

... major-effect loci based on their strong association with the phenotype in segregating populations. Although mapping strategies have been designed to detect minor-effect loci, many of these strategies have had poor success rates, potentially due to the confounding effects of major loci and epistasis. ...
Gene Section ETV6 (ets variant 6) Atlas of Genetics and Cytogenetics
Gene Section ETV6 (ets variant 6) Atlas of Genetics and Cytogenetics

Behavior Genetics of Prosocial Behavior
Behavior Genetics of Prosocial Behavior

... Using the quantitative genetics paradigm to partition the origins of a phenotype into genetic, shared environmental, and unique environmental sources has many advantages. Unlike much social science research, for example, this methodology enables researchers to make strong inferences about what cause ...
RT2 Profiler PCR Arrays: Pathway-focused gene
RT2 Profiler PCR Arrays: Pathway-focused gene

... pathway- or disease-specific genes: the RT2 Profiler PCR Array System. The RT2 Profiler PCR Array System combines the quantitative performance of SYBR® Green real-time PCR with the multiple-gene profiling capabilities of a microarray. The RT2 Profiler PCR Array is a 96- or 384-well plate containing ...
Accelerated Evolution of Sex Chromosomes in
Accelerated Evolution of Sex Chromosomes in

... Sundström et al. 2004; Geraldes et al. 2008; Ellegren 2009; see also table 1). In XY systems, the effective population size of the X is three-fourths that of the autosomes because under 1:1 sex ratio and equal variance in reproductive success between sexes, there are three copies of the X per matin ...
Document
Document

... simple sugars for use in seedling development. The “malting” of barley seeds in beer production is the process of using GA to induce enzymes in seed germination causing conversion of starches to sugars. Germination is then stopped by heating and the sugars are fermented. GA induces seedlessness in ...
Molecular Diagnostics 6
Molecular Diagnostics 6

... Viability is not much critical for molecular testing DNA and especially RNA can be damaged in lysed or nonviable cells ...
MAGMA manual (version 1.06)
MAGMA manual (version 1.06)

... serve as a reference for LD. Publically available data sets such as the 1,000 Genomes data set can be used for this purpose. The two main requirements for the reference data is that 1) there is a strong overlap between it and the SNP p-value input file in the SNPs it contains, since only SNPs that o ...
ACLS CH05 - CTCE Moodle
ACLS CH05 - CTCE Moodle

Plant Molecular Biology
Plant Molecular Biology

... the infection thread. Experiments using transcriptional lacZ fusions suggest that nodL belongs to the same transcriptional unit as nodF,E. Very low expression of nodL seems to be sufficient for biological activity. Introduction ...
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ABO Blood Group System
ABO Blood Group System

... He and his five co-workers began mixing each others red cells and serum together and inadvertently performed the first forward and reverse ABO groupings ...
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE

... Copyright © 2007 Pearson Education, Inc., publishing as Pearson Addison-Wesley ...
A novel duplication in the HOXA13 gene in a family with atypical
A novel duplication in the HOXA13 gene in a family with atypical

... whereas in affected males there is a high penetrance of hypospadias. Interestingly, there is no obvious reduced fertility in this large pedigree. Since low birth weight is known to affect the risk for hypospadias,2–5 we checked the birth weight of four affected males and they were all within normal ...
A plant dialect of the histone language
A plant dialect of the histone language

... biotinylation, which can all cause structural and functional rearrangements in chromatin and are therefore essential elements of the complex ‘epigenetic histone code’ [5,6]. To decipher this code, which is recognized and interpreted by transcriptional regulators and chromatin remodeling machines, is ...
Chapter 14
Chapter 14

... • cII and cIII cause repressor synthesis to be established. – They trigger inhibition of late gene transcription. ...
Albinism - OG
Albinism - OG

... from the mother both have to passed on to the kid.  The kid needs to have both albinism genes from each parent to have albinism, not just one.  If they only have one albino gene, then the child will be considered a “carrier” of an albinism gene.  Albinism is controlled by a recessive trait. ...
Specification of unique neuronal sub-types by integration of positional and temporal cues
Specification of unique neuronal sub-types by integration of positional and temporal cues

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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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