PDF
... deepens and begins to close on itself, the ectodermal snail-Am-expressing cells become elongated (Fig. 2A 18– 24 h, K) and eventually become internalised (Fig. 2A 18– 56 h, K–M). As the blastopore closes, the internalised cells lose their epithelial character (Fig. 2A 56 h, M). Thus, during the gast ...
... deepens and begins to close on itself, the ectodermal snail-Am-expressing cells become elongated (Fig. 2A 18– 24 h, K) and eventually become internalised (Fig. 2A 18– 56 h, K–M). As the blastopore closes, the internalised cells lose their epithelial character (Fig. 2A 56 h, M). Thus, during the gast ...
Using GO to improve text information access
... An ontology represents “what there is” in a domain. An ontology includes a vocabulary (which promotes a standard way of naming the concepts of the domain) and a system of hierarchical and other relations between and among the concepts and the vocabulary items. The ConverSpeech ontology – BioMedPlus ...
... An ontology represents “what there is” in a domain. An ontology includes a vocabulary (which promotes a standard way of naming the concepts of the domain) and a system of hierarchical and other relations between and among the concepts and the vocabulary items. The ConverSpeech ontology – BioMedPlus ...
Final Report
... vertebrate Sox5,6) in situ hybridization protocol for Limulus. Picking up the expression of SoxD using traditional whole mount protocols based on the colorimetric reaction by alkaline phosphatases has been elusive up to date. To troubleshoot, we tried to perform in situ hybridizations on sections of ...
... vertebrate Sox5,6) in situ hybridization protocol for Limulus. Picking up the expression of SoxD using traditional whole mount protocols based on the colorimetric reaction by alkaline phosphatases has been elusive up to date. To troubleshoot, we tried to perform in situ hybridizations on sections of ...
Inheritance of Aldehyde Oxidase in Drosophila melanogaster
... 1. To become familiar with Drosophila melanogaster as a genetic research tool. 2. To become familiar with certain biochemical aspects of genetic research. 3. To use biochemical tests to demonstrate some basic genetic principles. Enzyme Spot Test The trait that will be studied in each experiment is t ...
... 1. To become familiar with Drosophila melanogaster as a genetic research tool. 2. To become familiar with certain biochemical aspects of genetic research. 3. To use biochemical tests to demonstrate some basic genetic principles. Enzyme Spot Test The trait that will be studied in each experiment is t ...
factor occupancy and gene expression Effects of
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
Supplementary Materials and methods (doc 154K)
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
Figures and figure supplements
... from maternal brood chambers and then swim in the water column before they develop competence to settle and initiate metamorphosis into a juvenile. The juvenile body plan, which displays the hallmarks of the adult body plan, including an aquiferous system with canals, choanocytes chambers and oscula ...
... from maternal brood chambers and then swim in the water column before they develop competence to settle and initiate metamorphosis into a juvenile. The juvenile body plan, which displays the hallmarks of the adult body plan, including an aquiferous system with canals, choanocytes chambers and oscula ...
factor occupancy and gene expression Effects of sequence variation
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
factor occupancy and gene expression Effects of sequence variation
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
... A complex interplay between transcription factors (TFs) and the genome regulates transcription. However, connecting variation in genome sequence with variation in TF binding and gene expression is challenging due to environmental differences between individuals and cell types. To address this proble ...
alpha-amylase from bacillus licheniformis
... To construct the α-amylase production strain, three copies of the LE399 α-amylase gene were successively introduced into the chromosome of the SJ5550 host strain using conjugative plasmids carrying the LE399 gene flanked by either xyl, gnt, or amyL B. licheniformis chromosomal DNA sequences. As expe ...
... To construct the α-amylase production strain, three copies of the LE399 α-amylase gene were successively introduced into the chromosome of the SJ5550 host strain using conjugative plasmids carrying the LE399 gene flanked by either xyl, gnt, or amyL B. licheniformis chromosomal DNA sequences. As expe ...
univERsity oF copEnhAGEn
... region followed by the fibrinogen-like (FBG) domain. The collagen-like domain interacts with the MASPs and activates the lectin complement pathway [1]. The FBG domain enables the ficolins to bind to ligands [13]. Three identical polypeptide chains of 34–35 kDa are assembled into structural subunits ...
... region followed by the fibrinogen-like (FBG) domain. The collagen-like domain interacts with the MASPs and activates the lectin complement pathway [1]. The FBG domain enables the ficolins to bind to ligands [13]. Three identical polypeptide chains of 34–35 kDa are assembled into structural subunits ...
Molecular Pathology/Molecular Diagnostics/Genetic Testing
... Men rarely develop breast cancer and, thus, there may not be an affected first -degree relative, and the size of the family may not permit analysis of possible autosomal dominant inheritance. In patients with breast or ovarian cancer who are from high-risk families without a known BRCA1 or BRCA2 gen ...
... Men rarely develop breast cancer and, thus, there may not be an affected first -degree relative, and the size of the family may not permit analysis of possible autosomal dominant inheritance. In patients with breast or ovarian cancer who are from high-risk families without a known BRCA1 or BRCA2 gen ...
Disruption of mCry2 restores circadian rhythmicity in mPer2 mutant
... pression was back to a normal amplitude, reaching a maximum at CT6 in Per2Brdm1/mCry2−/− mutants (Fig. 2a). Thus, circadian expression of mPer1 in Per2Brdm1 mutants is rescued by inactivation of mCry2. A similar rescue was observed for mCry1 expression: both amplitude and timing of mCry1 oscillation ...
... pression was back to a normal amplitude, reaching a maximum at CT6 in Per2Brdm1/mCry2−/− mutants (Fig. 2a). Thus, circadian expression of mPer1 in Per2Brdm1 mutants is rescued by inactivation of mCry2. A similar rescue was observed for mCry1 expression: both amplitude and timing of mCry1 oscillation ...
Control of Lysogenization by Phage P22. II. Mutations (clyA) in the c1 Gene that Cause Increased Lysogenization
... tests are done under conditions in which each parent was infected at @I) into the ely-non-permissive host DB7000 and each parent carried a mutation in a P22 late gene. In every case. one parent carried a 5-am mutation carried an a-am mutation. In this way, only cells that were co-infected by each a ...
... tests are done under conditions in which each parent was infected at @I) into the ely-non-permissive host DB7000 and each parent carried a mutation in a P22 late gene. In every case. one parent carried a 5-am mutation carried an a-am mutation. In this way, only cells that were co-infected by each a ...
Analyzing the Changes in DNA Flexibility Due to Base Modifications
... after minimization, the volume will change too rapidly, causing the simulation to crash. This simulation will prevent that from occurring, but will be run at a pressure not consistent with experimental conditions. We will continue to restrain the DNA to its initial coordinates. This will simulate ar ...
... after minimization, the volume will change too rapidly, causing the simulation to crash. This simulation will prevent that from occurring, but will be run at a pressure not consistent with experimental conditions. We will continue to restrain the DNA to its initial coordinates. This will simulate ar ...
DNA Denaturing through UV-C Photon Dissipation: A
... RNA and DNA through the thermodynamically driven process of photon dissipation. Introduction Many of the fundamental molecules of life, those common to all three domains (bacteria, eukaryote, and archea), including RNA and DNA, amino acids, enzymes, vitamins, cofactors, and protoporphyrins, are pigm ...
... RNA and DNA through the thermodynamically driven process of photon dissipation. Introduction Many of the fundamental molecules of life, those common to all three domains (bacteria, eukaryote, and archea), including RNA and DNA, amino acids, enzymes, vitamins, cofactors, and protoporphyrins, are pigm ...
Efficiency of gene silencing in Arabidopsis
... and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs fac ...
... and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs fac ...
Chromosomal mutations
... • A break occuring during DNA replication or recombination event may remain unrepaired – Balances and unbalanced translocations, inversions → unbalanced gamete in meiosis → disease to progeny • Can be inherited, 60-70 % ...
... • A break occuring during DNA replication or recombination event may remain unrepaired – Balances and unbalanced translocations, inversions → unbalanced gamete in meiosis → disease to progeny • Can be inherited, 60-70 % ...
Package `LDheatmap`
... A character string to specify whether the provided map locations are in physical or genetic distances. If distances="physical" (default), the text describing the total length of the region will be “Physical Length:XXkb” where XX is the length of the region in kilobases. If distances="genetic", the t ...
... A character string to specify whether the provided map locations are in physical or genetic distances. If distances="physical" (default), the text describing the total length of the region will be “Physical Length:XXkb” where XX is the length of the region in kilobases. If distances="genetic", the t ...
Implications of Genetic Discrimination: Who Should Know What?
... individualized, effective and efficient medical treatment. A patient’s genetic information provides his doctor a more complete background of his condition. With this knowledge, the doctor is able to prescribe more appropriate, more effective treatment, including medications. With further progress in ...
... individualized, effective and efficient medical treatment. A patient’s genetic information provides his doctor a more complete background of his condition. With this knowledge, the doctor is able to prescribe more appropriate, more effective treatment, including medications. With further progress in ...
The Mitochondrial Genome of Chara vulgaris
... independently in several lineages. Of the 11 introns in Chaetosphaeridium mtDNA, only the fourth intron in cox1 exhibits positional and structural conservation with one of the seven introns in Mesostigma mtDNA, and only the second intron in the same gene is conserved positionally in land plant mtDNA ...
... independently in several lineages. Of the 11 introns in Chaetosphaeridium mtDNA, only the fourth intron in cox1 exhibits positional and structural conservation with one of the seven introns in Mesostigma mtDNA, and only the second intron in the same gene is conserved positionally in land plant mtDNA ...
DETECTION OF A RARE MUTATION IN FERROPORTIN GENE
... In this study we performed a HaloPlex™ capture with NGS platform analysis to screen simultaneously 5 hemochromatosis genes in a patient with a clear hemochromatosis phenotype (on the basis of age of onset, high serum ferritin, high transferrin saturation and hepatopathy) but without genetic diagnosi ...
... In this study we performed a HaloPlex™ capture with NGS platform analysis to screen simultaneously 5 hemochromatosis genes in a patient with a clear hemochromatosis phenotype (on the basis of age of onset, high serum ferritin, high transferrin saturation and hepatopathy) but without genetic diagnosi ...
Robust gene silencing mediated by antisense small RNAs in the
... the mechanism of silencing revealed that the newly generated AS sRNAs possess 50 -polyphosphate termini, map to introns indicating that they can be derived from nascent mRNA, are dependent on transcription of the trigger-gene fusion construct and persist after removal of the trigger plasmid, suggest ...
... the mechanism of silencing revealed that the newly generated AS sRNAs possess 50 -polyphosphate termini, map to introns indicating that they can be derived from nascent mRNA, are dependent on transcription of the trigger-gene fusion construct and persist after removal of the trigger plasmid, suggest ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse