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SVPaper050711
SVPaper050711

... transposable elements). Sequence based methods of SV detection, with higher ...
- Stabilis Fitness
- Stabilis Fitness

... and endurance, power, cardiovascular health and numerous other physiological variables (McCardle et al 1991; Powers & Howley 1990) is well accepted. The degree of physiological adaptation that results from training is now of more interest to us. As will be noted in the following reviews of exercise- ...
Alternative Splicing in Higher Plants
Alternative Splicing in Higher Plants

... For these alternative splicing isoforms that have a frameshift: - In Arabidopsis, 42% of these frameshifted events are found to introduce a premature stop codon >50bp upstream of the last exon-exon junction and are NMD candidates -In rice, this percentage is 36% This level of producing NMD candidate ...
GCF (T-14): sc-133419 - Santa Cruz Biotechnology
GCF (T-14): sc-133419 - Santa Cruz Biotechnology

... GCF (GC-rich sequence DNA-binding factor), also known as C2orf3 (chromosome 2 open reading frame 3), transcription factor 9 (TCF-9) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of β-Actin, EGFR and calciumdependent pr ...
Genetic or epigenetic difference causing discordance between
Genetic or epigenetic difference causing discordance between

... (KvDMR1) within the KCNQ1.40 In the other study, four of 37 cases with BWS was born by IVF, while IVF was identified as the method of conception in only one of 148 matched controls.41 Angelman syndrome and retinoblastoma are also reportedly associated with IVF.42 These findings suggest that IVF is a ...
Bioinformatics Dr. Víctor Treviño  Pabellón Tec
Bioinformatics Dr. Víctor Treviño Pabellón Tec

... One PAM is a unit of evolutionary divergence in which 1% of the amino acids have been changed in very similar sequences ...
Книжечка
Книжечка

... fertile offspring. We often find species described not by their reproduction (a biological species) but rather by their form (anatomical or form species). Populations: Groups of similar individuals who tend to mate with each other in a limited geographic area. This can be as simple as a field of flo ...
SYBR Green with low ROX
SYBR Green with low ROX

... repebody (Rb) mediated hot start method of Taq DNA polymerase. In the hot start method, engineered Taq DNA polymerse is combined with a repebody that specifically binds to the enzyme and inactivates until the first denaturation step. Thus, RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is suit ...
Nitrogen Fixation In Methanogens: The Archaeal
Nitrogen Fixation In Methanogens: The Archaeal

... belongs to a single operon, since transposons inserted throughout the cluster always had a polar effect, that is, they always eliminated the synthesis of mRNA from genes 3' to the insertion (20). Furthermore, an identifiable promoter sequence was present only in the region upstream of nifH. nif mRNA ...
Kinetic proofreading of gene activation by chromatin remodeling
Kinetic proofreading of gene activation by chromatin remodeling

... different targets on the nucleosome, one of which being H3 Lys 14Ac while the other is unknown. Both targets then together should provide sufficient binding energy. We believe that these findings are much simpler to explain in the context of our model. The explanations of the authors of the study re ...
Unexpected Complexity of Poly(A)-Binding Protein Gene Families in
Unexpected Complexity of Poly(A)-Binding Protein Gene Families in

... (Belostotsky and Meagher 1993, 1996; Chekanova et al. 2001; D. A. Belostotsky, unpublished data). PAB4 and -8 cDNA sequences (generated by SPP Consortium and RIKEN) were retrieved from GenBank via SIGnAL database links (http://signal.salk.edu). Exon predictions in the PAB6 and -7 genes are supported ...
The structure of a gene co-expression network reveals biological
The structure of a gene co-expression network reveals biological

... partial correlations [8]. Unlike in ontological enrichment analysis or bibliographic networks, information available on both functionally known and unknown genes is used for the network definition. Once the network is given, a full analysis of its structure could be performed, from either the point ...
Weak Selection and Protein Evolution
Weak Selection and Protein Evolution

... Mishima, Shizuoka 411-8540, Japan ...
The genus Listonella MacDonell and Colwell 1986 is a later
The genus Listonella MacDonell and Colwell 1986 is a later

... phenotypic data. The species of the genus Listonella were nested within the genus Vibrio according to the 16S rRNA gene sequence-based phylogenetic tree. The closest neighbour of Vibrio (Listonella) anguillarum strains LMG 4437T and ATCC 68554 (5strain 775) was Vibrio ordalii LMG 13544T, with more t ...
Inheritance and expression of transgenes in white clover
Inheritance and expression of transgenes in white clover

... Mendelian manner. However, non-Mendelian inheritance was also observed in the BC, progeny of some primary transgenics. This is not unusual and has been observed by others working with transgenic plants (Deroles & Gardner 1988; Peng et al. 1995). Variation was also noted in expression of the uidA gen ...
B.2 Specific Aims. The term `epigenetics` literally means `above the
B.2 Specific Aims. The term `epigenetics` literally means `above the

... modifications of gene expression potential[1]. DNA methylation is one molecular mechanism mediating epigenetic phenomena, and indicates the covalent transfer of a methyl group to the carbon at position 5 of cytosine residues,[2] usually within regions of DNA in which cytosine occurs next to a guanin ...
A Domestic cat X Chromosome Linkage Map and the Sex
A Domestic cat X Chromosome Linkage Map and the Sex

... Figure 1.—(A) Phenotypic variation at the orange locus. The top row depicts variation at the orange locus. Genotypes are presented for a female with representative coat color. From left to right: (a) nonorange, wild-type agouti; (b) nonorange, nonagouti; (c) orange heterozygote, nonagouti; (d) orang ...
Plant Functional Genomics Plant Functional Genomics
Plant Functional Genomics Plant Functional Genomics

... and E. coli as the host, making library construction and subsequent downstream procedures efficient and easy to perform. Recombinant DNA inserts up to 200 kb can be efficiently cloned and stably maintained in E. coli. Although the insert size cloning capacity is much lower than that of the YAC syste ...
Incontinentia Pigmenti
Incontinentia Pigmenti

... skin is presumed to reflect the clonal proliferation of t wo g e n e t i c a l l y d i ff er e n t c e l l t y p e s d u r i n g embryogenesis of the skin. Cellular mosaicism occurs in 46, XX females because of the random inactivation of one X chromosome (lyonization). The four different stages of I ...
ENZYME ACTIVITY
ENZYME ACTIVITY

... Enzymes are biological catalysts; that is, enzymes are able to mediate the conversion of substrate into product(s) without being changed or destroyed in the conversion process. Almost all enzymes are proteins. The only known exceptions are ribozymes, which are composed of RNA. Individual enzymes hav ...
Variations on a theme: Genomics of sex
Variations on a theme: Genomics of sex

... Zoological Institute, University of Basel, Vesalgasse 1, 4051 Basel, Switzerland Full list of author information is available at the end of the article ...
Role of virulence factors, cell components and adhesion in
Role of virulence factors, cell components and adhesion in

... shuttle mutants generated from the wild-type strains P12 and P1 were used in cocultivation assays. Isogenic mutants were used whenever possible. An overview of all strains used is given in Table 1. One important factor that might in£uence iNOS induction is the cag PAI. It contains various genes invo ...
MEIOSIS - sandsbiochem
MEIOSIS - sandsbiochem

... http://bestweekever.blogs.com/photos/uncategorized/imagemain_sperm_egg1_1.gif ...
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics

The Power of Memes - Dr Susan Blackmore
The Power of Memes - Dr Susan Blackmore

... based on any replicator, and so, as an example, he invented the idea of the meme. The copying of memes from one person to another is imperfect, just as the copying of genes from parent to child is sometimes inaccurate. We may embellish a story, forget a word of the song, adapt an old technology or c ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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