LOF1 and Interacting Transcription Factors in Plant Development
... Genotyping T-DNA Insertion Lines ...
... Genotyping T-DNA Insertion Lines ...
SURVEY AND SUMMARY H1 histones
... (90,91). Conversely, overexpression of murine histone H1c gives rise to an increase in or no change in transcription levels, while conferring no effect on cell cycle progression (91). These overexpression experiments show functional differences between the two variants, although additional experimen ...
... (90,91). Conversely, overexpression of murine histone H1c gives rise to an increase in or no change in transcription levels, while conferring no effect on cell cycle progression (91). These overexpression experiments show functional differences between the two variants, although additional experimen ...
1 sirt1 is involved in glucocorticoid-mediated control of
... JBC Papers in Press. Published on September 20, 2007 as Manuscript M707114200 The latest version is at http://www.jbc.org/cgi/doi/10.1074/jbc.M707114200 ...
... JBC Papers in Press. Published on September 20, 2007 as Manuscript M707114200 The latest version is at http://www.jbc.org/cgi/doi/10.1074/jbc.M707114200 ...
Transcription
... including an ATPase, a helicase, and a kinase activity that can phosphorylate the CTD tail of RNA polymerase II; it is also involved in repair of damage to DNA. Phosphorylation of the CTD ...
... including an ATPase, a helicase, and a kinase activity that can phosphorylate the CTD tail of RNA polymerase II; it is also involved in repair of damage to DNA. Phosphorylation of the CTD ...
Evolvement of LEM proteins as chromatin tethers at the nuclear
... preferential tethering of intergenic regions with repressive histone marks at the NE [19]. This led to the concept that LADs (lamina-associated domains), whose molecular properties remain elusive, mediate anchoring of genomic regions to the periphery and keep genes in a transcriptionally ‘locked’ st ...
... preferential tethering of intergenic regions with repressive histone marks at the NE [19]. This led to the concept that LADs (lamina-associated domains), whose molecular properties remain elusive, mediate anchoring of genomic regions to the periphery and keep genes in a transcriptionally ‘locked’ st ...
Phenotypic Modulation of Smooth Muscle Cells
... We then infected A7r5 cells with an adenovirus encoding Foxo4-TMN (Adtet-off-Foxo4-TMN) (Figures 2D and 2E). Twenty-four hours after infection, cells were serum starved and stimulated with IGF-I in the presence or absence of LY294002. Lysates were harvested 1 hr later for Western blot (Figure 2D) an ...
... We then infected A7r5 cells with an adenovirus encoding Foxo4-TMN (Adtet-off-Foxo4-TMN) (Figures 2D and 2E). Twenty-four hours after infection, cells were serum starved and stimulated with IGF-I in the presence or absence of LY294002. Lysates were harvested 1 hr later for Western blot (Figure 2D) an ...
Noise in eukaryotic gene expression
... codon adaptation indices (0.183 and 0.115, respectively), the level of noise in GFPmut3b expression can, when assuming similar mRNA decay rates, be used as an indicator of tetR gene expression noise. By using a value of 4 AU for tetR gene expression noise in the simulations, we overestimate represso ...
... codon adaptation indices (0.183 and 0.115, respectively), the level of noise in GFPmut3b expression can, when assuming similar mRNA decay rates, be used as an indicator of tetR gene expression noise. By using a value of 4 AU for tetR gene expression noise in the simulations, we overestimate represso ...
Studies of focal adhesion assembly
... Fn3. High-resolution structures of many of these component domains and domain combinations have been available for some time [6]; the main remaining uncertainties are associated with how Fn forms complexes with its various partners. Fn has conformational flexibility that exposes different binding si ...
... Fn3. High-resolution structures of many of these component domains and domain combinations have been available for some time [6]; the main remaining uncertainties are associated with how Fn forms complexes with its various partners. Fn has conformational flexibility that exposes different binding si ...
Initiation of DNA replication in eukaryotes: questioning the origin
... PCR method [14]. This 747 bp origin is functional in a variety of human cells [15] and appears therefore ubiquitous. It lies in a constitutively expressed gene domain, like many origins mapped so far, and protein complexes appear to associate with the origin in a cell cycle-dependent manner [16]. At ...
... PCR method [14]. This 747 bp origin is functional in a variety of human cells [15] and appears therefore ubiquitous. It lies in a constitutively expressed gene domain, like many origins mapped so far, and protein complexes appear to associate with the origin in a cell cycle-dependent manner [16]. At ...
How do non-enyzmatic domains become enzymes
... and alkylated base methylases, like AlkB, the DNA repair enzyme ...
... and alkylated base methylases, like AlkB, the DNA repair enzyme ...
HeLa cells
... Western blots commonly used to detect activated proteins. Typically use anti-phosho… antibodies for detection of phosphorylated protein, on a nylon membrane that are marked and a picture is taken. ...
... Western blots commonly used to detect activated proteins. Typically use anti-phosho… antibodies for detection of phosphorylated protein, on a nylon membrane that are marked and a picture is taken. ...
Non-protein-coding RNA
... Cell growth and proliferation are processes in the cell that must be tightly regulated. Transcription of ribosomal RNA and ribosomal biogenesis are directly linked to cell growth and proliferation, since the ribosomal RNA encodes for the majority of transcription in a cell and ribosomal biogenesis i ...
... Cell growth and proliferation are processes in the cell that must be tightly regulated. Transcription of ribosomal RNA and ribosomal biogenesis are directly linked to cell growth and proliferation, since the ribosomal RNA encodes for the majority of transcription in a cell and ribosomal biogenesis i ...
NEW EMBO MEMBER`S REVIEW Nuclear and cytosolic events of
... All of the 10 identified COP/DET/FUS genes operate by directly or indirectly repressing the transcription of light-inducible genes (Misera et al., 1994; Kwok et al., 1995) and thereby repressing the default pathway of photomorphogenesis in darkness. Interestingly, the COP/ DET/FUS genes seem to be e ...
... All of the 10 identified COP/DET/FUS genes operate by directly or indirectly repressing the transcription of light-inducible genes (Misera et al., 1994; Kwok et al., 1995) and thereby repressing the default pathway of photomorphogenesis in darkness. Interestingly, the COP/ DET/FUS genes seem to be e ...
COMMENTARY REDOX SIGNALLING BY TRANSCRIPTION
... domains contain also cysteine residues whose oxidation state affects DNA binding. Such redox-sensitive cysteines have been found in the basic leucine zipper proteins c-Fos and c-Jun, the helix-loop-helix transcription factor USF, the zinc finger protein Egr-l, and other factors, such as c-Myb and p5 ...
... domains contain also cysteine residues whose oxidation state affects DNA binding. Such redox-sensitive cysteines have been found in the basic leucine zipper proteins c-Fos and c-Jun, the helix-loop-helix transcription factor USF, the zinc finger protein Egr-l, and other factors, such as c-Myb and p5 ...
Chromatin plasticity in pluripotent cells
... is organized via H1, a linker histone protein, into a tight helical organization, which is compacted further into complex high-order structures that are not yet fully understood. Chromatin structure is controlled by various protein complexes generally called chromatin modifiers, affecting DNA access ...
... is organized via H1, a linker histone protein, into a tight helical organization, which is compacted further into complex high-order structures that are not yet fully understood. Chromatin structure is controlled by various protein complexes generally called chromatin modifiers, affecting DNA access ...
DNA Replication Origin Function Is Promoted by H3K4 Di
... ABSTRACT DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiate ...
... ABSTRACT DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiate ...
Applications of recombinant DNA technology in
... This discussion of the transcriptional control of gene expression focuses on the role of RNA polymerase II, the enzyme responsible for transcribing protein-encoding genes into mRNAs (9,12-14). The production of each mRNA in human cells involves complex interactions of proteins (ie, trans-acting fact ...
... This discussion of the transcriptional control of gene expression focuses on the role of RNA polymerase II, the enzyme responsible for transcribing protein-encoding genes into mRNAs (9,12-14). The production of each mRNA in human cells involves complex interactions of proteins (ie, trans-acting fact ...
1 DNA replication origin function is promoted by H3K4 di
... DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiated a gene ...
... DNA replication is a highly regulated process that is initiated from replication origins, but the elements of chromatin structure that contribute to origin activity have not been fully elucidated. To identify histone post-translational modifications important for DNA replication, we initiated a gene ...
Gene-Centered Regulatory Network Mapping
... their sequence-specific DNA binding domain and can be grouped into families based on the type of DNA binding domain they possess (Reece-Hoyes et al., 2005). Well-known DNA binding domains include the homeodomain, the basic helix-loop-helix (bHLH) domain, C2H2 zinc fingers, the ETS domain, the bZIP d ...
... their sequence-specific DNA binding domain and can be grouped into families based on the type of DNA binding domain they possess (Reece-Hoyes et al., 2005). Well-known DNA binding domains include the homeodomain, the basic helix-loop-helix (bHLH) domain, C2H2 zinc fingers, the ETS domain, the bZIP d ...
Small GTPases
... • regulation of the active transport between the nucleus and cytoplasm (TFs, histons from the cytoplasm to the nucleus, tRNA and mRNA vice versa) RanGAP is in the cytoplasm RanGTP is converted here to RanGDP RanGEF is in the nucleus RanGDP is converted here to RanGTP transport from the cyt ...
... • regulation of the active transport between the nucleus and cytoplasm (TFs, histons from the cytoplasm to the nucleus, tRNA and mRNA vice versa) RanGAP is in the cytoplasm RanGTP is converted here to RanGDP RanGEF is in the nucleus RanGDP is converted here to RanGTP transport from the cyt ...
3 - Rudner Lab - Harvard University
... The translation of genetic information into form during development is guided by a complex interplay between genes and their protein products. Morphogenesis in systems ranging in complexity from bacteria to the mammalian embryo is characterized by the expression of sets of genes in highly ordered te ...
... The translation of genetic information into form during development is guided by a complex interplay between genes and their protein products. Morphogenesis in systems ranging in complexity from bacteria to the mammalian embryo is characterized by the expression of sets of genes in highly ordered te ...
Chapter 2
... Figure 02.25B: Nuclear pore complexes are channels that penetrate the inner and outer nuclear membrane and regulate the traffic into and out of the nucleus. Photo courtesy of Terry Allen, University of Manchester. ...
... Figure 02.25B: Nuclear pore complexes are channels that penetrate the inner and outer nuclear membrane and regulate the traffic into and out of the nucleus. Photo courtesy of Terry Allen, University of Manchester. ...
Histone acetylation and deacetylation
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.