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De Robertis 1.pm
... BMP, Chordin, Xolloid, Tolloid and Twisted gastrulation. Chordin, the molecule that generates the pattern, is a BMP antagonist that contains four cysteine rich (CR) domains that bind to BMP, blocking its binding to the receptor. Tolloid/Xolloid encodes a metalloproteinase that cleaves Chordin at two ...
... BMP, Chordin, Xolloid, Tolloid and Twisted gastrulation. Chordin, the molecule that generates the pattern, is a BMP antagonist that contains four cysteine rich (CR) domains that bind to BMP, blocking its binding to the receptor. Tolloid/Xolloid encodes a metalloproteinase that cleaves Chordin at two ...
Table S1.
... Rv3923c catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'leader sequence and broadening the substrate speci ...
... Rv3923c catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'leader sequence and broadening the substrate speci ...
The histidine triad protein Hint1 interacts with Pontin and Reptin and
... (Date et al., 2001; Moreira et al., 2001) and the scavenger mRNA decapping enzyme DcpS (Liu et al., 2002). Less is known about the physiological and cellular functions of Hint1/PKCI. The identification of Hint1/PKCI interaction partners suggested that it might be involved in the regulation of transc ...
... (Date et al., 2001; Moreira et al., 2001) and the scavenger mRNA decapping enzyme DcpS (Liu et al., 2002). Less is known about the physiological and cellular functions of Hint1/PKCI. The identification of Hint1/PKCI interaction partners suggested that it might be involved in the regulation of transc ...
Dynamic modification of the ETS transcription factor PEA3 by
... the potential interplay between these two modifications. We demonstrate that PEA3 is acetylated in a p300-dependent manner. ERK MAPK pathway signalling potentiates acetylation of PEA3, and enhances its trans-activation capacity. However, the major acetylation and sumoylation events take place on the ...
... the potential interplay between these two modifications. We demonstrate that PEA3 is acetylated in a p300-dependent manner. ERK MAPK pathway signalling potentiates acetylation of PEA3, and enhances its trans-activation capacity. However, the major acetylation and sumoylation events take place on the ...
cAMP-Dependent Protein Kinase, Catalytic Subunit Product
... online catalog for the most up-to-date information on Promega products. ...
... online catalog for the most up-to-date information on Promega products. ...
The orphan histidine protein kinase SgmT is a cdiGMP receptor and
... A fundamental property of bacterial cells is their ability to respond to changes in the extracellular and intracellular environments. In this context, specific inputs are coupled to specific outputs by dynamically interacting signalling proteins organized in regulatory pathways. In order to ensure a ...
... A fundamental property of bacterial cells is their ability to respond to changes in the extracellular and intracellular environments. In this context, specific inputs are coupled to specific outputs by dynamically interacting signalling proteins organized in regulatory pathways. In order to ensure a ...
Phosphorylation-Dependent Targeting of Tetrahymena HP1 to
... versus H3K27 methyl-lysine binding activities of HP1 and Pc family proteins that were previously identified through structural analyses of Drosophila and mammalian homologs (11, 12, 14). CD sequences from Hhp1, dHp1a, Drosophila Pc (dPc), human Cbx1-8, and the HP1 homolog Swi6 from fission yeast were ...
... versus H3K27 methyl-lysine binding activities of HP1 and Pc family proteins that were previously identified through structural analyses of Drosophila and mammalian homologs (11, 12, 14). CD sequences from Hhp1, dHp1a, Drosophila Pc (dPc), human Cbx1-8, and the HP1 homolog Swi6 from fission yeast were ...
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... 20.2 Eukaryotic RNA polymerases consist of many subunits 20.3 Promoter elements are defined by mutations and footprinting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a univers ...
... 20.2 Eukaryotic RNA polymerases consist of many subunits 20.3 Promoter elements are defined by mutations and footprinting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a univers ...
PDF document
... degrade non-soluble cellulose components of cell walls, specifically parts composed of crystalline cellulose. The latter represents the most recalcitrant form of cellulose due to its highly-ordered structure, in which adjacent chains of cellodextrines (polymers of D-glucose) in cellulose sheets are ...
... degrade non-soluble cellulose components of cell walls, specifically parts composed of crystalline cellulose. The latter represents the most recalcitrant form of cellulose due to its highly-ordered structure, in which adjacent chains of cellodextrines (polymers of D-glucose) in cellulose sheets are ...
Chromatin organization and global regulation of Hox gene clusters
... The epigenetic regulation of Hox gene clusters seems to rely, mostly, on the activities of protein complexes encoded by Polycomb (PcG) and Trithorax (TrxG) group genes. During early Drosophila embryogenesis, maternally supplied transcription factors define the spatial patterns of Hox genes’ activity ...
... The epigenetic regulation of Hox gene clusters seems to rely, mostly, on the activities of protein complexes encoded by Polycomb (PcG) and Trithorax (TrxG) group genes. During early Drosophila embryogenesis, maternally supplied transcription factors define the spatial patterns of Hox genes’ activity ...
ClgR regulation of chaperone and protease systems is essential for
... apparatus. In M. tuberculosis, the latter system has been shown to degrade proteins that have been post-translationally tagged with small protein modifiers in a system analogous to eukaryote ubiquitination (Darwin, 2009). The M. tuberculosis ClpP and FtsH enzymes appear to be essential cellular comp ...
... apparatus. In M. tuberculosis, the latter system has been shown to degrade proteins that have been post-translationally tagged with small protein modifiers in a system analogous to eukaryote ubiquitination (Darwin, 2009). The M. tuberculosis ClpP and FtsH enzymes appear to be essential cellular comp ...
A Few Good Domains
... The RING finger is a specialized type of Zn finger consisting of 40–60 residues that binds two atoms of zinc, and is involved in mediating protein—protein interactions. The presence of a RING finger domain is a characteristic of RING-class E3 ubiquitin protein ligases capable of transfering ubiquiti ...
... The RING finger is a specialized type of Zn finger consisting of 40–60 residues that binds two atoms of zinc, and is involved in mediating protein—protein interactions. The presence of a RING finger domain is a characteristic of RING-class E3 ubiquitin protein ligases capable of transfering ubiquiti ...
mRNA
... In eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. The most common type of core promoter in eukaryotes is a short DNA sequence known as a TATA box, found -30 base pairs from the start site of transcription. The ...
... In eukaryotes, RNA polymerase, and therefore the initiation of transcription, requires the presence of a core promoter sequence in the DNA. The most common type of core promoter in eukaryotes is a short DNA sequence known as a TATA box, found -30 base pairs from the start site of transcription. The ...
Bioinformatics analysis of experimentally determined protein
... Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2002), Ho et al. (Ho 2002), and those collected in the MIPS database (Mewes 2002). In addition, when we computationally simulate subunit compositions identical in numbers with those identi ...
... Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2002), Ho et al. (Ho 2002), and those collected in the MIPS database (Mewes 2002). In addition, when we computationally simulate subunit compositions identical in numbers with those identi ...
Nucleosomes released from oviduct nuclei during brief micrococcal
... gel (SDS or acid-urea). In the SDS two-dimensional analysis a vertical s t r i p ( 1 cm wide) of the DNP gel was equilibrated for 2 hours in 2.3% SDS, 6.25 mM Tris-HCl (pH 6.8), 10% glycerol, 5% mercaptoethanol and then placed on top of the stacking gel of the SDS-slab gel using the system described ...
... gel (SDS or acid-urea). In the SDS two-dimensional analysis a vertical s t r i p ( 1 cm wide) of the DNP gel was equilibrated for 2 hours in 2.3% SDS, 6.25 mM Tris-HCl (pH 6.8), 10% glycerol, 5% mercaptoethanol and then placed on top of the stacking gel of the SDS-slab gel using the system described ...
Involvement of a High-Mobility-Group Protein in the Transcriptional
... important for promoter function. The mutation causing the most significant reduction in expression alters a stretch of 23 thymidine residues (T23) that binds a protein with several properties common to high-mobilitygroup (HMG) proteins. The T23 binding activity is heat stable, can be inhibited by po ...
... important for promoter function. The mutation causing the most significant reduction in expression alters a stretch of 23 thymidine residues (T23) that binds a protein with several properties common to high-mobilitygroup (HMG) proteins. The T23 binding activity is heat stable, can be inhibited by po ...
Regulation of components of the Pseudomonas aeruginosa
... Phosphate transport has been examined in several other bacterial species and recent evidence suggests that a phosphate-starvation-inducible regulon, similar to that found in £. coli, exists in these other species. For example, a PhoB analogue has been identified in Baciitus subtilis. This gene produ ...
... Phosphate transport has been examined in several other bacterial species and recent evidence suggests that a phosphate-starvation-inducible regulon, similar to that found in £. coli, exists in these other species. For example, a PhoB analogue has been identified in Baciitus subtilis. This gene produ ...
Heterodimerization of the Two Motor Subunits of the Heterotrimeric
... containing only SpKRP95 or SpKRP85, respectively. However when SpKRP85 and SpKRP95 were expressed then incubated together in a cell-free lysate, SpKRP85 was immunoprecipitated together with SpKRP95 by the T7 tag antibody (Figure 3 lane 5), suggesting that SpKRP85 must have bound to SpKRP95 in the ly ...
... containing only SpKRP95 or SpKRP85, respectively. However when SpKRP85 and SpKRP95 were expressed then incubated together in a cell-free lysate, SpKRP85 was immunoprecipitated together with SpKRP95 by the T7 tag antibody (Figure 3 lane 5), suggesting that SpKRP85 must have bound to SpKRP95 in the ly ...
Enhancement of Nuclear Factor- B Acetylation by Coactivator p300
... subunits, NF-B1 (p50), the DNA-binding subunit, and RelA (p65), the transactivating subunit. NF-B transcription factors bind to 10-bp DNA sites (B sites) as dimers. They bind specifically to a decameric sequence (5⬘-GGGACTTTCC-3⬘) in the light chain enhancer region (3). The activity of NF-B is ...
... subunits, NF-B1 (p50), the DNA-binding subunit, and RelA (p65), the transactivating subunit. NF-B transcription factors bind to 10-bp DNA sites (B sites) as dimers. They bind specifically to a decameric sequence (5⬘-GGGACTTTCC-3⬘) in the light chain enhancer region (3). The activity of NF-B is ...
In Vitro Translation Systems – Protein expression
... The 1-Step Human High-Yield IVT Kits are cell-free protein expression systems that provide all of the essential components required for transcription and translation. The kits are optimized with Accessory Proteins and Reaction Mixes that support protein synthesis using a DNA template. The advantages ...
... The 1-Step Human High-Yield IVT Kits are cell-free protein expression systems that provide all of the essential components required for transcription and translation. The kits are optimized with Accessory Proteins and Reaction Mixes that support protein synthesis using a DNA template. The advantages ...
Identification and characterization of a cluster of transcription start
... initiation of transcription is independent of a TATA-box element, which is consistent with the finding of multiple transcription start sites. Furthermore, it is shown that proteins from HeLa and SiHa nuclear cell extracts bind to the two regions at nt 291–314 and 388–411, and that these two regions ...
... initiation of transcription is independent of a TATA-box element, which is consistent with the finding of multiple transcription start sites. Furthermore, it is shown that proteins from HeLa and SiHa nuclear cell extracts bind to the two regions at nt 291–314 and 388–411, and that these two regions ...
Basic Principles of Transcription and Translation
... of the polypeptides of hemoglobin. The numbers under the RNA refer to the codons. β globin is 146 amino acids long. The β globin gene and its pre mRNA transcript have three exons corresponding to sequences that will leave the nucleus as RNA. (The 5’ UTR and 3’ UTR are parts of exons because they are ...
... of the polypeptides of hemoglobin. The numbers under the RNA refer to the codons. β globin is 146 amino acids long. The β globin gene and its pre mRNA transcript have three exons corresponding to sequences that will leave the nucleus as RNA. (The 5’ UTR and 3’ UTR are parts of exons because they are ...
Three multidomain esterases from the cellulolytic
... (44 %) and belong to carbohydrate esterase family 3 ; both domains are shown here to be capable of deacetylating acetylated xylans, but no evidence was found for ferulic acid esterase activity. The esterase domain of XynE, however, shares 42 % amino acid identity with a family 1 phenolic acid estera ...
... (44 %) and belong to carbohydrate esterase family 3 ; both domains are shown here to be capable of deacetylating acetylated xylans, but no evidence was found for ferulic acid esterase activity. The esterase domain of XynE, however, shares 42 % amino acid identity with a family 1 phenolic acid estera ...
Histone acetylation and deacetylation
![](https://commons.wikimedia.org/wiki/Special:FilePath/Nucleosome_1KX5_colour_coded.png?width=300)
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.