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sv-lncs - Department of Computer Science and Engineering
... Definition of correlated sequence signature: when a sequence signature appears for frequent than random among interacting proteins. They claim this method is just an ...
... Definition of correlated sequence signature: when a sequence signature appears for frequent than random among interacting proteins. They claim this method is just an ...
The Dock and Lock Method: A Novel
... human carcinoembryonic antigen (CEACAM5). The first A, C-DDD1-Fab-hMN-14, was generated by linking the DDD1 peptide sequence, which is composed of amino acids 1 to 44 of human RIIa, to the COOH-terminal end of the Fd chain via a 14-residue flexible peptide linker (Fig. 2B). This construct was modifi ...
... human carcinoembryonic antigen (CEACAM5). The first A, C-DDD1-Fab-hMN-14, was generated by linking the DDD1 peptide sequence, which is composed of amino acids 1 to 44 of human RIIa, to the COOH-terminal end of the Fd chain via a 14-residue flexible peptide linker (Fig. 2B). This construct was modifi ...
Transcriptional regulation of the H-ras1 proto
... by members from the jun family (jun, junB, junD), and cFos proteins are active components and contribute to the activity of AP-1 by forming c-Jun homodimers as well as c-Jun-c-Fos heterodimers. In addition, there appears to be a mutual antagonism between activation by AP-1 and glucocorticoid recepto ...
... by members from the jun family (jun, junB, junD), and cFos proteins are active components and contribute to the activity of AP-1 by forming c-Jun homodimers as well as c-Jun-c-Fos heterodimers. In addition, there appears to be a mutual antagonism between activation by AP-1 and glucocorticoid recepto ...
... of post-transcriptional processing of primary transcripts of pre-mRNAs in eukaryotes. RNA splicing - removal of non-coding intron (intervening) sequences, between coding exon (expressed) sequences. Capping of 5' end (i.e., 7-methylquanosine) and addition of poly A tail to 3' end of pre-mRNA. In euka ...
The Xanthomonas Type III Effector XopD Targets the
... to nuclear foci and, when coexpressed with MYB30-CFP, induced MYB30 relocalization to these subnuclear structures (Figure 2D). In addition, all XopD mutants were able to interact with MYB30 in the nucleus (Table 1) and repressed MYB30 transcriptional activation of KCS1p, although this effect was wea ...
... to nuclear foci and, when coexpressed with MYB30-CFP, induced MYB30 relocalization to these subnuclear structures (Figure 2D). In addition, all XopD mutants were able to interact with MYB30 in the nucleus (Table 1) and repressed MYB30 transcriptional activation of KCS1p, although this effect was wea ...
The Characterization of the Olf-1/EBF
... .90% identity over a stretch of 370 residues suggested to be essential for dimerization and DNA binding (Hagman et al., 1995). The HLH dimerization motif of these proteins is located at the carboxyl end of this highly conserved region, and each displays a high degree of identity between helix 1 and ...
... .90% identity over a stretch of 370 residues suggested to be essential for dimerization and DNA binding (Hagman et al., 1995). The HLH dimerization motif of these proteins is located at the carboxyl end of this highly conserved region, and each displays a high degree of identity between helix 1 and ...
Transcription (genetics)
... (5-carbon) sugar where DNA has deoxyribose (one less oxygen atom) in its sugar-phosphate backbone). Unlike DNA replication, mRNA transcription can involve multiple RNA polymerases on a single DNA template and multiple rounds of transcription (amplification of particular mRNA), so many mRNA molecules ...
... (5-carbon) sugar where DNA has deoxyribose (one less oxygen atom) in its sugar-phosphate backbone). Unlike DNA replication, mRNA transcription can involve multiple RNA polymerases on a single DNA template and multiple rounds of transcription (amplification of particular mRNA), so many mRNA molecules ...
Regulation cascade of flagellar expression in Gram‐negative bacteria
... are two-component regulatory systems for class III genes that include £agellar ¢lament genes £iC and £aA, in Pseudomonadaceae and Vibrionaceae, respectively. Another class II gene, £iA, encodes the c28 £agellar-speci¢c factor participating in the transcription of class IV genes, such as non-essentia ...
... are two-component regulatory systems for class III genes that include £agellar ¢lament genes £iC and £aA, in Pseudomonadaceae and Vibrionaceae, respectively. Another class II gene, £iA, encodes the c28 £agellar-speci¢c factor participating in the transcription of class IV genes, such as non-essentia ...
Chapter 18
... • Some transcription factors function as repressors, inhibiting expression of a particular gene • Some activators and repressors act indirectly by influencing chromatin structure to promote ...
... • Some transcription factors function as repressors, inhibiting expression of a particular gene • Some activators and repressors act indirectly by influencing chromatin structure to promote ...
review - University of Oxford
... factors,16 and the other for longer through the sheer size of active polymerizing complexes, which can contain a multisubunit enzyme, nascent RNA, and associated proteins such as ribosomes in bacteria or spliceosomes in eukaryotes.17,18 In Fig. 1b, neighbouring transcription units a and b are attach ...
... factors,16 and the other for longer through the sheer size of active polymerizing complexes, which can contain a multisubunit enzyme, nascent RNA, and associated proteins such as ribosomes in bacteria or spliceosomes in eukaryotes.17,18 In Fig. 1b, neighbouring transcription units a and b are attach ...
Nucleosome depletion at yeast terminators is not
... leading to the suggestion that intrinsic histone–DNA interactions appear to be more important for nucleosome depletion at terminator regions in vivo (13, 14). The conclusion that intrinsic histone–DNA interactions are important for nucleosome depletion at promoters and terminators is based on the si ...
... leading to the suggestion that intrinsic histone–DNA interactions appear to be more important for nucleosome depletion at terminator regions in vivo (13, 14). The conclusion that intrinsic histone–DNA interactions are important for nucleosome depletion at promoters and terminators is based on the si ...
Lecture 25
... was carried out by means of HRP-linked anti-human IgG. This study detected p53 autoantibodies by means of NAPPA microarrays, which was confirmed by ELISA. The p53 levels were found to be directly related to tumour burden with serum antibody concentration decreasing after neoadjuvant chemotherapy. Pr ...
... was carried out by means of HRP-linked anti-human IgG. This study detected p53 autoantibodies by means of NAPPA microarrays, which was confirmed by ELISA. The p53 levels were found to be directly related to tumour burden with serum antibody concentration decreasing after neoadjuvant chemotherapy. Pr ...
Genome Biology - Rodrigo A. Gutierrez Laboratory
... macronutrients, both C and N metabolites can act as signals that influence many cellular processes through regulation of gene expression in plants [1-6] and other organisms (e.g. [7, 8]). In plants, C and N metabolites can regulate developmental processes such as flowering time [9] and root architec ...
... macronutrients, both C and N metabolites can act as signals that influence many cellular processes through regulation of gene expression in plants [1-6] and other organisms (e.g. [7, 8]). In plants, C and N metabolites can regulate developmental processes such as flowering time [9] and root architec ...
CHAPTER 19 Regulation of Gene Expression in Bacteria and
... (housekeeping genes). Examples include protein synthesis and glucose metabolism. 3. All genes are regulated at some level, so that as resources dwindle the cell can respond with a different molecular strategy. 4. Prokaryotic genes are often organized into operons that are cotranscribed. A regulatory ...
... (housekeeping genes). Examples include protein synthesis and glucose metabolism. 3. All genes are regulated at some level, so that as resources dwindle the cell can respond with a different molecular strategy. 4. Prokaryotic genes are often organized into operons that are cotranscribed. A regulatory ...
Nuclear Factor-90 of Activated T-Cells: A Double
... DNA-binding activity and transcriptional activation to the immunosuppressant drugs cyclosporin A and FK506 (31, 32). Both subunits of the heterodimeric complex have been cloned and found to consist of subunits of Mr ) 45 000 (NF45) and Mr ) 90 000 (NF90). The phosphorylation state of this ARRE-2 bin ...
... DNA-binding activity and transcriptional activation to the immunosuppressant drugs cyclosporin A and FK506 (31, 32). Both subunits of the heterodimeric complex have been cloned and found to consist of subunits of Mr ) 45 000 (NF45) and Mr ) 90 000 (NF90). The phosphorylation state of this ARRE-2 bin ...
Kinetics of MPF and histone H1 kinase activity differ during the G2
... the definitive first metaphase plate is associated both with high MPF and histone Hi kinase activity. As to whether either of these activities is causally related to chromatin condensation and bivalent formation is unproven. That a direct causal relationship may indeed not exist is suggested by evid ...
... the definitive first metaphase plate is associated both with high MPF and histone Hi kinase activity. As to whether either of these activities is causally related to chromatin condensation and bivalent formation is unproven. That a direct causal relationship may indeed not exist is suggested by evid ...
The scs Boundary Element: Characterization of Boundary Element
... been shown to occur only in conjunction with the clustered binding sites from near the 59 long terminal repeat of the gypsy retrotransposon and thus may be a special situation. Also, evidence argues against mediation of scs or scs9 boundary activity by clustered binding sites for a single protein. I ...
... been shown to occur only in conjunction with the clustered binding sites from near the 59 long terminal repeat of the gypsy retrotransposon and thus may be a special situation. Also, evidence argues against mediation of scs or scs9 boundary activity by clustered binding sites for a single protein. I ...
Protein-Protein Interactions: Stability, Function and Landscape
... homodimer cytochrome c9 (1)) (Fig. 1a). Heterocomplexes can also have such properties, or they can be non-obligatory, being made and broken according to the environment or external factors and involve proteins that must also exist independently [e.g., the enzyme–inhibitor complex trypsin with the in ...
... homodimer cytochrome c9 (1)) (Fig. 1a). Heterocomplexes can also have such properties, or they can be non-obligatory, being made and broken according to the environment or external factors and involve proteins that must also exist independently [e.g., the enzyme–inhibitor complex trypsin with the in ...
Abiotic stress response in plants: When post
... and heavy metals has been well established [26]. However, proof of their role in abiotic stress tolerance as RNA chaperones has been only recently shown for two Arabidopsis glycine-rich proteins, GRP2 and AtRZ-1a [27,28]. Both proteins accelerate seed germination and seedling growth under cold stres ...
... and heavy metals has been well established [26]. However, proof of their role in abiotic stress tolerance as RNA chaperones has been only recently shown for two Arabidopsis glycine-rich proteins, GRP2 and AtRZ-1a [27,28]. Both proteins accelerate seed germination and seedling growth under cold stres ...
No Slide Title
... – utilizes highly engineered tet and reverse tet proteins to get specific effects properties of the system – what happens when one puts proteins into the cell that respond differently to the same effector compound? • If they can dimerize with each other • can not dimerize with each other • or if the ...
... – utilizes highly engineered tet and reverse tet proteins to get specific effects properties of the system – what happens when one puts proteins into the cell that respond differently to the same effector compound? • If they can dimerize with each other • can not dimerize with each other • or if the ...
TRANSCRIPTION. The process of RNA synthesis directed by a DNA
... Eukaryotes. Compared with prokaryotes, very little is known about how eukaryotes terminate transcription. a. RNA polymerase I terminates transcription in a factor-dependent manner at a particular sequence. b. RNA polymerase III terminates transcription by an unknown mechanism after the synthesis of ...
... Eukaryotes. Compared with prokaryotes, very little is known about how eukaryotes terminate transcription. a. RNA polymerase I terminates transcription in a factor-dependent manner at a particular sequence. b. RNA polymerase III terminates transcription by an unknown mechanism after the synthesis of ...
Flies with disrupted clock (per 01 )
... (ROS) to the side chains of amino acid residues in specific proteins and enzymes in animals [3]. Studies involving protein damage and aging have shown that the rate of protein oxidation increases dramatically during the last third of an organism’s life span [4]. This suggests that protein damage due ...
... (ROS) to the side chains of amino acid residues in specific proteins and enzymes in animals [3]. Studies involving protein damage and aging have shown that the rate of protein oxidation increases dramatically during the last third of an organism’s life span [4]. This suggests that protein damage due ...
Chapter 18 Gene Expression
... Initiation of Translation • The initiation of translation of selected mRNAs can be blocked by regulatory proteins that bind to sequences or structures of the ...
... Initiation of Translation • The initiation of translation of selected mRNAs can be blocked by regulatory proteins that bind to sequences or structures of the ...
Regulation of Gene Expression
... Initiation of Translation • The initiation of translation of selected mRNAs can be blocked by regulatory proteins that bind to sequences or structures of the ...
... Initiation of Translation • The initiation of translation of selected mRNAs can be blocked by regulatory proteins that bind to sequences or structures of the ...
The Copines, a Novel Class of C2 Domain-containing, Calcium
... vesicles were resuspended in 10 ml of extracting buffer (25 mM HEPESNaOH, pH 7.4, 10 mM EGTA, 50 mM phenylmethylsulfonyl fluoride) and sedimented as above. The supernatant, containing calcium-dependent lipid-binding proteins, was saved. The lipid pellet was resuspended in extracting buffer again and ...
... vesicles were resuspended in 10 ml of extracting buffer (25 mM HEPESNaOH, pH 7.4, 10 mM EGTA, 50 mM phenylmethylsulfonyl fluoride) and sedimented as above. The supernatant, containing calcium-dependent lipid-binding proteins, was saved. The lipid pellet was resuspended in extracting buffer again and ...
Histone acetylation and deacetylation
![](https://commons.wikimedia.org/wiki/Special:FilePath/Nucleosome_1KX5_colour_coded.png?width=300)
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.