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... The histone chaperone CAF-1 is known for its role in DNA replication-coupled histone deposition. However, loss of function causes lethality only in higher multicellular organisms such as mice and flies, but not in unicellular organisms such as yeasts, suggesting that CAF-1 has other important functi ...
... The histone chaperone CAF-1 is known for its role in DNA replication-coupled histone deposition. However, loss of function causes lethality only in higher multicellular organisms such as mice and flies, but not in unicellular organisms such as yeasts, suggesting that CAF-1 has other important functi ...
Zygotic Genome Activation During the Maternal-to
... Identification of de novo zygotic transcription. (a) In the early embryo, the maternal contribution (red ) represents the majority of the transcripts present. This can mask the relatively small amount of de novo zygotic transcription (blue) and lead to detection difficulties. Experimental techniques t ...
... Identification of de novo zygotic transcription. (a) In the early embryo, the maternal contribution (red ) represents the majority of the transcripts present. This can mask the relatively small amount of de novo zygotic transcription (blue) and lead to detection difficulties. Experimental techniques t ...
In vitro analysis of the VE-cadherin promoter - HAL
... that the Sp1 site as well as EBS 2 and 3 interact with specific transcription factors in human endothelial cells and thus participate in VE-cadherin gene transcription. Database search revealed consensus binding sites for zinc finger transcription factors (underlined in Fig. 3B). These CT boxes may ...
... that the Sp1 site as well as EBS 2 and 3 interact with specific transcription factors in human endothelial cells and thus participate in VE-cadherin gene transcription. Database search revealed consensus binding sites for zinc finger transcription factors (underlined in Fig. 3B). These CT boxes may ...
AHM1, a Novel Type of Nuclear Matrix–Localized
... The nuclear matrix, operationally defined, is the dynamic fibrogranular structure forming the skeletal framework that surrounds and penetrates the interphase nucleus; it has been implicated in most nuclear functions, including replication, repair, transcription, RNA processing, and RNA transport (Be ...
... The nuclear matrix, operationally defined, is the dynamic fibrogranular structure forming the skeletal framework that surrounds and penetrates the interphase nucleus; it has been implicated in most nuclear functions, including replication, repair, transcription, RNA processing, and RNA transport (Be ...
Slide 1
... The lac operon is an inducible operon and contains genes that code for enzymes used in the hydrolysis and metabolism of lactose By itself, the lac repressor is active and switches the lac operon off A molecule called an inducer inactivates the repressor to turn the lac operon on ...
... The lac operon is an inducible operon and contains genes that code for enzymes used in the hydrolysis and metabolism of lactose By itself, the lac repressor is active and switches the lac operon off A molecule called an inducer inactivates the repressor to turn the lac operon on ...
Brooker Chapter 14
... Corepressors bind to repressors and cause them to bind to DNA Inhibitors bind to activators and prevent them from binding to DNA Genes that are regulated in this manner are termed repressible Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... Corepressors bind to repressors and cause them to bind to DNA Inhibitors bind to activators and prevent them from binding to DNA Genes that are regulated in this manner are termed repressible Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
Functional studies of the BTB domain in the Drosophila GAGA and
... genes that encode BTB domain-containing proteins with roles in transcription regulation (25,26). trl encodes GAGA protein (26). GAGA was first identified as a factor that stimulates in vitro transcription of the Drosophila engrailed and Ultrabithorax promoters (27,28). Subsequent studies have shown ...
... genes that encode BTB domain-containing proteins with roles in transcription regulation (25,26). trl encodes GAGA protein (26). GAGA was first identified as a factor that stimulates in vitro transcription of the Drosophila engrailed and Ultrabithorax promoters (27,28). Subsequent studies have shown ...
Control of cell cycle transcription during G1 and S phases
... Cells commit to enter a new cell cycle during G1 by activating cyclin–CDK-dependent transcription (FIG. 1). G1–S transcriptional activation during late G1 promotes entry into S phase after which expression is turned off. This creates a wave of transcription, which peaks at the G1‑to‑S transition (BO ...
... Cells commit to enter a new cell cycle during G1 by activating cyclin–CDK-dependent transcription (FIG. 1). G1–S transcriptional activation during late G1 promotes entry into S phase after which expression is turned off. This creates a wave of transcription, which peaks at the G1‑to‑S transition (BO ...
Gene Regulation
... Blocks the transcription of the β-galactosidase gene It does that by binding to the Lactose operator and getting ...
... Blocks the transcription of the β-galactosidase gene It does that by binding to the Lactose operator and getting ...
Structural and enzymatic characterization of a glycoside hydrolase
... 6.80 (Dionex) using a Dionex Carbopac PA200 column. Solvent A was water, solvent B was 1 M sodium hydroxide and solvent C was 1 M sodium acetate. Depending on the analytes, the following gradients were employed. Gradient A: 0–5 min, 10 % solvent B and 2 % solvent C; 5–12 min, 10 % B and a linear gra ...
... 6.80 (Dionex) using a Dionex Carbopac PA200 column. Solvent A was water, solvent B was 1 M sodium hydroxide and solvent C was 1 M sodium acetate. Depending on the analytes, the following gradients were employed. Gradient A: 0–5 min, 10 % solvent B and 2 % solvent C; 5–12 min, 10 % B and a linear gra ...
Yeast Ty1 Retrotransposition Is Stimulated by a Synergistic
... terms of alterations in chromatin structure is proposed. ments), and have very similar internal regions. The more distantly related retrotransposons Ty3, Ty4, and Ty5 have different sets of LTRs, are present in lower copy numbers, and have not been observed to be insertional mutagens. Yeast retrotra ...
... terms of alterations in chromatin structure is proposed. ments), and have very similar internal regions. The more distantly related retrotransposons Ty3, Ty4, and Ty5 have different sets of LTRs, are present in lower copy numbers, and have not been observed to be insertional mutagens. Yeast retrotra ...
Separation of nuclear protein complexes by blue native
... against actins, Pol I, Pol II, UBF, snRNPs, and NDH II (Fig. 2) showed that except labile actin complexes, the complexes of other tested proteins were fairly stable. The specificity of antibody labeling on native gel blots was confirmed by 2-D BN/SDS-PAGE, where the coordinates of detected protein o ...
... against actins, Pol I, Pol II, UBF, snRNPs, and NDH II (Fig. 2) showed that except labile actin complexes, the complexes of other tested proteins were fairly stable. The specificity of antibody labeling on native gel blots was confirmed by 2-D BN/SDS-PAGE, where the coordinates of detected protein o ...
Testis-specific TAF homologs collaborate to control a
... raising the possibility that cell-type- or stage-specific forms of what was previously thought of as the general transcription machinery may play an important role in selective activation of certain PolII promoters (Verrijzer, 2001). To date, however, only a few tissue-specific TAFIIs have been inve ...
... raising the possibility that cell-type- or stage-specific forms of what was previously thought of as the general transcription machinery may play an important role in selective activation of certain PolII promoters (Verrijzer, 2001). To date, however, only a few tissue-specific TAFIIs have been inve ...
Supplement Supporting Materials and Methods Site
... unlabeled wild-type probe (A) or mutant probe A (ASBE3m) with the functional SBE mutant were used as competitors; lane 5-6, unlabeled wild-type SBE oligonucleotides were used as competitors; lane 7-8, unlabeled mutant SEB oligonucleotides were used as competitors. Indicated are the specific complex ...
... unlabeled wild-type probe (A) or mutant probe A (ASBE3m) with the functional SBE mutant were used as competitors; lane 5-6, unlabeled wild-type SBE oligonucleotides were used as competitors; lane 7-8, unlabeled mutant SEB oligonucleotides were used as competitors. Indicated are the specific complex ...
Abscisic Acid–Induced Transcription Is Mediated by
... however, it retained the ability to respond to ABA. These results indicate that region II but not region III is essential for mediating ABA signal. Because we did not test a deletion of the N-terminal 20 amino acids of the truncated version of TRAB1, it is possible that this part of region I also mi ...
... however, it retained the ability to respond to ABA. These results indicate that region II but not region III is essential for mediating ABA signal. Because we did not test a deletion of the N-terminal 20 amino acids of the truncated version of TRAB1, it is possible that this part of region I also mi ...
Centronuclear myopathy in mice lacking a novel muscle
... genes directly, or it may act indirectly by controlling the expression of subordinate transcription factors or signaling molecules that act as intermediaries to connect MEF2 to downstream targets that themselves are not dependent on MEF2-binding sites in their cis-regulatory regions. Indeed, vast ar ...
... genes directly, or it may act indirectly by controlling the expression of subordinate transcription factors or signaling molecules that act as intermediaries to connect MEF2 to downstream targets that themselves are not dependent on MEF2-binding sites in their cis-regulatory regions. Indeed, vast ar ...
Klug, A., The discovery of Zinc fingers and Their Application in Gene Regulation and Genome Manipulation . Ann. Rev. Biochem. 79, 213-231 (2010).
... amino acids in the repeat fold around a Zn ion to form a small independent structural domain or module, the “finger,” and the five intervening amino acids provide the linkers between consecutive fingers (Figure 2b). The Zn ion forms the basis of the folding by being tetrahedrally coordinated to the two ...
... amino acids in the repeat fold around a Zn ion to form a small independent structural domain or module, the “finger,” and the five intervening amino acids provide the linkers between consecutive fingers (Figure 2b). The Zn ion forms the basis of the folding by being tetrahedrally coordinated to the two ...
coIP就是co-immunoprecipitation,假如有两种蛋白质A,B相互作用
... step depends on whether the researcher is confirming previously suspected protein:protein interactions or identifying unknown protein:protein interactions. The Pull-Down Assay as a Confirmatory Tool Confirmation of previously suspected interactions typically utilizes a prey protein source that has b ...
... step depends on whether the researcher is confirming previously suspected protein:protein interactions or identifying unknown protein:protein interactions. The Pull-Down Assay as a Confirmatory Tool Confirmation of previously suspected interactions typically utilizes a prey protein source that has b ...
The Role of Chromosome Domains in Shaping the
... conformation capture (3C). Collectively, these studies demonstrated a correlation between chromatin topology and underlying gene activity, without resolving whether chromosome folding is a cause or consequence of genomic functions (Cavalli and Misteli, 2013; de Laat and Duboule, 2013). Topology and ...
... conformation capture (3C). Collectively, these studies demonstrated a correlation between chromatin topology and underlying gene activity, without resolving whether chromosome folding is a cause or consequence of genomic functions (Cavalli and Misteli, 2013; de Laat and Duboule, 2013). Topology and ...
A CHASE domain containing protein kinase OsCRL4
... and that have intracellular histidine-kinase or nucleotide cyclase domain, the domain has been named the CHASE domain (cyclases/histidinekinase-associated sensory extracellular) (Alexander and Thomas, 2003). In Arabidopsis, the CHASE domain is specific for AHK2, AHK3 and CRE1/AHK4 (Alexander and Tho ...
... and that have intracellular histidine-kinase or nucleotide cyclase domain, the domain has been named the CHASE domain (cyclases/histidinekinase-associated sensory extracellular) (Alexander and Thomas, 2003). In Arabidopsis, the CHASE domain is specific for AHK2, AHK3 and CRE1/AHK4 (Alexander and Tho ...
Lecture 15: Processing of viral pre-mRNA
... • Can occur co-transcriptionally: – Copying of a long polyU stretch in template RNA: picornaviruses, M virus of yeast – Reiteritive copying of short U stretches in template RNA: Ortho- and Paramyxoviruses ...
... • Can occur co-transcriptionally: – Copying of a long polyU stretch in template RNA: picornaviruses, M virus of yeast – Reiteritive copying of short U stretches in template RNA: Ortho- and Paramyxoviruses ...
Life and death of transcriptional co
... cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigenetic regulathrough the alternative autophagosome pathway. Many nuclear tion, and shed molecular insights into the roles of cellular trafproteins including p300 are substrate of aggresome ...
... cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigenetic regulathrough the alternative autophagosome pathway. Many nuclear tion, and shed molecular insights into the roles of cellular trafproteins including p300 are substrate of aggresome ...
Histone acetylation and deacetylation
![](https://commons.wikimedia.org/wiki/Special:FilePath/Nucleosome_1KX5_colour_coded.png?width=300)
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.