Single-molecule DNA biochemistry, genetic circuits and chromatin
... transcription My research interest is based on two different ideas, both formulated about 40 years ago: At the Cold Spring Harbor Symposium 1961, Jacob and Monod commented that “it is obvious from the analysis of these [bacterial genetic regulatory] mechanisms that their known elements could be conn ...
... transcription My research interest is based on two different ideas, both formulated about 40 years ago: At the Cold Spring Harbor Symposium 1961, Jacob and Monod commented that “it is obvious from the analysis of these [bacterial genetic regulatory] mechanisms that their known elements could be conn ...
Single Cell Analysis of RNA-mediated Histone H3.3 Transcription Site
... Histone H3.3 Is Recruited through an RNA-mediated Mechanism resembles the RI variant (3). However, the essential function of RI H3 chromatin assembly is still not well understood. Initially, H3.3 was thought to regulate transcriptionally active chromatin because the first reports were of its incorp ...
... Histone H3.3 Is Recruited through an RNA-mediated Mechanism resembles the RI variant (3). However, the essential function of RI H3 chromatin assembly is still not well understood. Initially, H3.3 was thought to regulate transcriptionally active chromatin because the first reports were of its incorp ...
Determination of the Binding Site-Size of the Protein
... crude cell extracts, it is thought that cell extracts contain many other DNA-binding proteins (and their regulatory proteins), and some small molecules (such as ATP and DNA metabolites), that are also able to interact with the DNA and the target protein; these molecules may cause some unpredictable ...
... crude cell extracts, it is thought that cell extracts contain many other DNA-binding proteins (and their regulatory proteins), and some small molecules (such as ATP and DNA metabolites), that are also able to interact with the DNA and the target protein; these molecules may cause some unpredictable ...
The Distribution of Polycomb-Group Proteins During Cell Division
... the mechanism of PcG-mediated repression in Drosophila will have broad ramifications with respect to the problem of developmental control in higher eukaryotes. However, neither a description of the molecular interactions by which PcG repression is established nor how this is maintained through cell ...
... the mechanism of PcG-mediated repression in Drosophila will have broad ramifications with respect to the problem of developmental control in higher eukaryotes. However, neither a description of the molecular interactions by which PcG repression is established nor how this is maintained through cell ...
Protein Expression - New England Biolabs
... translation. Express a wide range of proteins free of modification or degradation by simply mixing two tubes followed by the addition of template DNA. With results available in only a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies. Choose from seve ...
... translation. Express a wide range of proteins free of modification or degradation by simply mixing two tubes followed by the addition of template DNA. With results available in only a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies. Choose from seve ...
DNA Binding Site Sequence Directs Glucocorticoid
... Sebastiaan H. Meijsing,1* Miles A. Pufall,1* Alex Y. So,1,2 Darren L. Bates,3 Lin Chen,3 Keith R. Yamamoto1,2† Genes are not simply turned on or off, but instead their expression is fine-tuned to meet the needs of a cell. How genes are modulated so precisely is not well understood. The glucocorticoi ...
... Sebastiaan H. Meijsing,1* Miles A. Pufall,1* Alex Y. So,1,2 Darren L. Bates,3 Lin Chen,3 Keith R. Yamamoto1,2† Genes are not simply turned on or off, but instead their expression is fine-tuned to meet the needs of a cell. How genes are modulated so precisely is not well understood. The glucocorticoi ...
fulltext
... The human genome consists of about 3 billion bases. Each cell contains several genes. The number of the genes, their duplication and patterns of gene expression and the fact that our bodies contain several hundred cell types make very complex biological systems. The cell has evolved many strategies ...
... The human genome consists of about 3 billion bases. Each cell contains several genes. The number of the genes, their duplication and patterns of gene expression and the fact that our bodies contain several hundred cell types make very complex biological systems. The cell has evolved many strategies ...
Fan et al.P65
... critical domains that are thought to mediate its proposed function as a transcriptional repressor. The three domains are: 1) the methyl CpG binding domain (MBD) that confers binding to methylated CpG dinucleotides [23]; 2) a transcriptional repression domain (TRD), which interacts with various co-re ...
... critical domains that are thought to mediate its proposed function as a transcriptional repressor. The three domains are: 1) the methyl CpG binding domain (MBD) that confers binding to methylated CpG dinucleotides [23]; 2) a transcriptional repression domain (TRD), which interacts with various co-re ...
Signal Transduction From the Endoplasmic Reticulum to the Cell
... In addition to the transcription factor HAC1, a second component of the UPR signal transduction pathway has been described. Genetic analyses have identified a Ser/Thr kinase (Ire1p/Ern1p) whose activity is required for induction of the UPR in yeast (18, 58). Ire1p acts upstream of Hac1p, since the H ...
... In addition to the transcription factor HAC1, a second component of the UPR signal transduction pathway has been described. Genetic analyses have identified a Ser/Thr kinase (Ire1p/Ern1p) whose activity is required for induction of the UPR in yeast (18, 58). Ire1p acts upstream of Hac1p, since the H ...
Poster - Protein Information Resource
... As the volume of scientific literature rapidly grows, literature data mining becomes increasingly critical to facilitate genome/proteome annotation and to improve the quality of biological databases. Annotations derived from experimentally verified data from literature are of special value to the Un ...
... As the volume of scientific literature rapidly grows, literature data mining becomes increasingly critical to facilitate genome/proteome annotation and to improve the quality of biological databases. Annotations derived from experimentally verified data from literature are of special value to the Un ...
Depletion of MOM1 in nondividing cells of
... (Rougier et al., 1998), mitotic activity is an important factor for the release of silencing by this mechanism. However, a recent study of chromatin structure in quiescent Drosophila cells provided evidence that heterochromatic regions become accessible for transcription (Ahmad and Henikoff, 2001) a ...
... (Rougier et al., 1998), mitotic activity is an important factor for the release of silencing by this mechanism. However, a recent study of chromatin structure in quiescent Drosophila cells provided evidence that heterochromatic regions become accessible for transcription (Ahmad and Henikoff, 2001) a ...
Promoters
... polymerase to bind to the promoter. This closed complex then converts to an open promoter complex. CAP-cAMP causes recruitment through protein-protein interactions, by bending the DNA, or by a combination of these phenomena. ...
... polymerase to bind to the promoter. This closed complex then converts to an open promoter complex. CAP-cAMP causes recruitment through protein-protein interactions, by bending the DNA, or by a combination of these phenomena. ...
File - Tay
... (1) What is the GO for the proteins involved in the degradation of GM2 in the HEXA interaction network? Method: Using the AMIGO database to find out the gene ontology of each related proteins in the degradation of GM2 and categorize them into different cellular component. ...
... (1) What is the GO for the proteins involved in the degradation of GM2 in the HEXA interaction network? Method: Using the AMIGO database to find out the gene ontology of each related proteins in the degradation of GM2 and categorize them into different cellular component. ...
File - Tay
... (1) What is the GO for the proteins involved in the degradation of GM2 in the HEXA interaction network? Method: Using the AMIGO database to find out the gene ontology of each related proteins in the degradation of GM2 and categorize them into different cellular component. ...
... (1) What is the GO for the proteins involved in the degradation of GM2 in the HEXA interaction network? Method: Using the AMIGO database to find out the gene ontology of each related proteins in the degradation of GM2 and categorize them into different cellular component. ...
Links between genome replication and chromatin landscapes
... the specification of genomic sites where ORIs are located, their coordinated timing of activation and the coupling with other DNA-mediated processes (transcription, repair, recombination) are still very poorly understood in molecular terms. An idea is emerging that to understand the process not only ...
... the specification of genomic sites where ORIs are located, their coordinated timing of activation and the coupling with other DNA-mediated processes (transcription, repair, recombination) are still very poorly understood in molecular terms. An idea is emerging that to understand the process not only ...
... proteasome. Together with the 20S core particle they form the intact 26S proteasome of about 2.5 MDa. The 19S regulatory subcomplexes of the proteasome unfold the protein substrates and assist in their translocation through a narrow gate into the 20S core particle where degradation takes place. The ...
Redox Homeostasis and Signaling - University of Nebraska–Lincoln
... Oxygen toxicity was mitigated during evolution by the development of oxidative stress defense systems and signaling systems for the control of intracellular ROS. The level of ROS is controlled not only by environmental and metabolic processes but also by ROS generation. ROS-based signaling that invo ...
... Oxygen toxicity was mitigated during evolution by the development of oxidative stress defense systems and signaling systems for the control of intracellular ROS. The level of ROS is controlled not only by environmental and metabolic processes but also by ROS generation. ROS-based signaling that invo ...
lecture07_13
... • Domains are long motifs (30-100 aa) and are considered as the building blocks of proteins (evolutionary modules). ...
... • Domains are long motifs (30-100 aa) and are considered as the building blocks of proteins (evolutionary modules). ...
Data Supplement
... buffer prior to isolation for real-time PCR. The primers used to assess NFATc1 binding to the Rcan1.4 promoter amplified a 65 bp fragment between –282 and –217 in a region with the highest conservation between human, mouse and frog. Primers used for the Rcan1.4 promoter were: forward 5’TGGGAACTATGCC ...
... buffer prior to isolation for real-time PCR. The primers used to assess NFATc1 binding to the Rcan1.4 promoter amplified a 65 bp fragment between –282 and –217 in a region with the highest conservation between human, mouse and frog. Primers used for the Rcan1.4 promoter were: forward 5’TGGGAACTATGCC ...
ppt
... Transcription factors can inhibit or encourage the binding of the RNA Polymerase. And, through signal transduction, environmental factors can influence the activity of these transcription factors. So cells can respond genetically to changes in their environment. ...
... Transcription factors can inhibit or encourage the binding of the RNA Polymerase. And, through signal transduction, environmental factors can influence the activity of these transcription factors. So cells can respond genetically to changes in their environment. ...
Choosing between pathogenicity and saprophytism: A case study
... were ambiguously classified based on BLAST results, were blasted on the basis of the information provided by individual members of a particular contig (singletons). ESTs and contigs with ambiguous classifications or annotations were aligned with the draft genome sequence of the R. solani isolate AG3 ...
... were ambiguously classified based on BLAST results, were blasted on the basis of the information provided by individual members of a particular contig (singletons). ESTs and contigs with ambiguous classifications or annotations were aligned with the draft genome sequence of the R. solani isolate AG3 ...
Elements involved in light regulation of the parsley chs promoter: cis
... A family of related cis-acting elements contains an ACGT core It seems to be more the rule than the exception that similar cis-acting elements are present in a variety of promoters which are regulated by diverse stimuli. A prominent example in mammalian systems is an element with the consensus T;cT/ ...
... A family of related cis-acting elements contains an ACGT core It seems to be more the rule than the exception that similar cis-acting elements are present in a variety of promoters which are regulated by diverse stimuli. A prominent example in mammalian systems is an element with the consensus T;cT/ ...
operons operons operons
... cAMP makes CAP active; starts transcription Image from:http://image.slidesharecdn.com/18regulationofgeneexpression-130613012903-phpapp02/95/18-regulation-of-gene-expression-15-638.jpg?cb=1371087103 ...
... cAMP makes CAP active; starts transcription Image from:http://image.slidesharecdn.com/18regulationofgeneexpression-130613012903-phpapp02/95/18-regulation-of-gene-expression-15-638.jpg?cb=1371087103 ...
CDPKs – a kinase for every Ca signal?
... domains have some sequence similarity to calmodulin (20% identity), but their EF-hands are poorly conserved. Representative members of this group appear to be unresponsive to calcium8–10. It is not known how these protein kinases are regulated or what their physiological roles are. Another type of C ...
... domains have some sequence similarity to calmodulin (20% identity), but their EF-hands are poorly conserved. Representative members of this group appear to be unresponsive to calcium8–10. It is not known how these protein kinases are regulated or what their physiological roles are. Another type of C ...
Cellular transcription factors that interact with p6 promoter elements
... Institut fu$ r Medizinische Mikrobiologie und Hygiene, Universita$ t Regensburg, Franz-Josef-Strauß-Allee 11, D-93053 Regensburg, Germany ...
... Institut fu$ r Medizinische Mikrobiologie und Hygiene, Universita$ t Regensburg, Franz-Josef-Strauß-Allee 11, D-93053 Regensburg, Germany ...
Histone acetylation and deacetylation
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.