Sensitive Detection of Botulinum Neurotoxin Type A
... could be used to capture BoNT/A from complex matrices. Methods Used: Magnetic beads coated with a polyclonal antibody specific for BoNT/A heavy chain, List Prod #730, were used to capture and concentrate the toxin prior to addition of the substrate, FRET peptide SNAPtide®, List Prod #520. Various bu ...
... could be used to capture BoNT/A from complex matrices. Methods Used: Magnetic beads coated with a polyclonal antibody specific for BoNT/A heavy chain, List Prod #730, were used to capture and concentrate the toxin prior to addition of the substrate, FRET peptide SNAPtide®, List Prod #520. Various bu ...
Cloning and Characterization of Human Urocortin
... to type 1 and type 2 CRF receptorsand, basedupon its anatomiclocalization within the brain, was proposedto be a natural ligand for the type 2 CRF receptors. Using a genomiclibrary, we have cloned the humancounterpart of rat urocortin and localized it to humanchromosome2. Humanand rat urocortin share ...
... to type 1 and type 2 CRF receptorsand, basedupon its anatomiclocalization within the brain, was proposedto be a natural ligand for the type 2 CRF receptors. Using a genomiclibrary, we have cloned the humancounterpart of rat urocortin and localized it to humanchromosome2. Humanand rat urocortin share ...
Selective Binding of Lisinopril in Angiotensin
... Angiotensin-converting enzyme (ACE) is a membrane-anchored carboxypeptidase enzyme that cleaves both angiotensin I and bradykinin. Cleavage of angiotensin I yields angiotensin II, and ultimately results in potent vasoconstriction and high blood pressure. Bradykinin acts as a vasodilator, and cleavag ...
... Angiotensin-converting enzyme (ACE) is a membrane-anchored carboxypeptidase enzyme that cleaves both angiotensin I and bradykinin. Cleavage of angiotensin I yields angiotensin II, and ultimately results in potent vasoconstriction and high blood pressure. Bradykinin acts as a vasodilator, and cleavag ...
Thermodynamic and Biophysical Characterization of Cytochrome P450
... or ligand concentration. Amax is the maximal absorption shift obtained at saturation with substrate or ligand, S, Et is the total enzyme concentration used, and Kd is the apparent dissociation constant for the substrate- or ligand-enzyme complex. Binding of azole drugs (ketoconazole, miconazole, eco ...
... or ligand concentration. Amax is the maximal absorption shift obtained at saturation with substrate or ligand, S, Et is the total enzyme concentration used, and Kd is the apparent dissociation constant for the substrate- or ligand-enzyme complex. Binding of azole drugs (ketoconazole, miconazole, eco ...
Cloning of adiponectin receptors that mediate antidiabetic metabolic
... cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocytes (Supplementary ...
... cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocytes (Supplementary ...
Natural cannabinoids: Templates for drug discovery
... compounds, known to exist only in this plant. There are 66 known plant-derived cannabinoids, the most prevalent of which are the tetrahydrocannabinols (THCs), the cannabidiols (CBDs), and the cannabinols (CBNs). The next most abundant cannabinoids are the cannabigerols (CBGs), the cannabichromenes ( ...
... compounds, known to exist only in this plant. There are 66 known plant-derived cannabinoids, the most prevalent of which are the tetrahydrocannabinols (THCs), the cannabidiols (CBDs), and the cannabinols (CBNs). The next most abundant cannabinoids are the cannabigerols (CBGs), the cannabichromenes ( ...
Supramolecular Chemistry—Scope and Perspectives Molecules
... chemistry, not limited to transition-metal ions but extending to all types of substrates: cationic, anionic, or neutral species of organic, inorganic, or biological nature. In order to achieve high recognition it is desirable that receptor and substrate be in contact over a large area. This occurs w ...
... chemistry, not limited to transition-metal ions but extending to all types of substrates: cationic, anionic, or neutral species of organic, inorganic, or biological nature. In order to achieve high recognition it is desirable that receptor and substrate be in contact over a large area. This occurs w ...
Creatine kinase: The reactive cysteine is required for synergism but
... of the pH optimum to lower values was observed. Mutant Mib-CKs were severalfold more sensitive to inhibition by free ADP in the reverse reaction (ATP synthesis) and to free ATP in the forward reaction (phosphocreatine synthesis). With the exception of C278D, all mutant enzymes were specifically acti ...
... of the pH optimum to lower values was observed. Mutant Mib-CKs were severalfold more sensitive to inhibition by free ADP in the reverse reaction (ATP synthesis) and to free ATP in the forward reaction (phosphocreatine synthesis). With the exception of C278D, all mutant enzymes were specifically acti ...
CASE 19
... is readily reversible and is in equilibrium. Thus, not only does O2 binding or release cause a change in [H+], the H+ concentration influences O2 binding by hemoglobin. Although seemingly minor, the O2 binding affinity of hemoglobin is pH-dependent and is important to the overall physiologic functio ...
... is readily reversible and is in equilibrium. Thus, not only does O2 binding or release cause a change in [H+], the H+ concentration influences O2 binding by hemoglobin. Although seemingly minor, the O2 binding affinity of hemoglobin is pH-dependent and is important to the overall physiologic functio ...
13- and 14-membered macrocyclic ligands containing
... and teta) or methylphosphonate (tritp and tetp) arms were determined. All the ligands were labelled with 153Sm and 166Ho in order to evaluate the effect of the macrocyclic cavity size and type of appended arms on their in vitro and in vivo behaviour. The radiolabelling efficiency was found to be higher ...
... and teta) or methylphosphonate (tritp and tetp) arms were determined. All the ligands were labelled with 153Sm and 166Ho in order to evaluate the effect of the macrocyclic cavity size and type of appended arms on their in vitro and in vivo behaviour. The radiolabelling efficiency was found to be higher ...
Enzyme Mechanisms - Illinois Institute of Technology
... Several mitochondrial catabolic enzyme act on substrates produced in the cytoplasm; these require elaborate transport mechanisms to move them in Therefore, control of the transporters confers control over the enzymatic system ...
... Several mitochondrial catabolic enzyme act on substrates produced in the cytoplasm; these require elaborate transport mechanisms to move them in Therefore, control of the transporters confers control over the enzymatic system ...
DESIGNING OF A POTENT ANALOG AGAINST DRUG RESISTANT HIV-1 PROTEASE:... STUDY Research Article
... proteins. The necessity of this enzyme (HIV-1 Protease) for the maturation of provirus and completion of virus life cycle makes it a promising target for Anti-Retroviral therapy. Fortunately for HIV virus and unfortunately for humans the viral reverse transcriptase enzyme lacks proofreading activity ...
... proteins. The necessity of this enzyme (HIV-1 Protease) for the maturation of provirus and completion of virus life cycle makes it a promising target for Anti-Retroviral therapy. Fortunately for HIV virus and unfortunately for humans the viral reverse transcriptase enzyme lacks proofreading activity ...
Structure of ATP-Bound Human ATP:Cobalamin
... methylmalonyl CoA to succinyl CoA and is responsible for normal catabolism of various amino and fatty acids through the tricarboxylic acid (TCA) cycle (6). The conversion from CNCbl to the active cofactors requires carrier proteins, membrane transport, cobalt reduction, and exchange of the axial lig ...
... methylmalonyl CoA to succinyl CoA and is responsible for normal catabolism of various amino and fatty acids through the tricarboxylic acid (TCA) cycle (6). The conversion from CNCbl to the active cofactors requires carrier proteins, membrane transport, cobalt reduction, and exchange of the axial lig ...
Applied Biosystems Real-Time PCR Rapid Assay Development
... For real-time PCR assays designed following our Rapid Assay Development Guidelines, we recommend final reaction concentrations of 900nM for each primer and 250nM of the probe for Gene Expression, and 900nM of each primer and 200nM of each probe for SNP Genotyping assays. These recommendations will p ...
... For real-time PCR assays designed following our Rapid Assay Development Guidelines, we recommend final reaction concentrations of 900nM for each primer and 250nM of the probe for Gene Expression, and 900nM of each primer and 200nM of each probe for SNP Genotyping assays. These recommendations will p ...
transition metal complexes of amino acid and peptide derivatives
... formation processes has already been widely studied. However the presence of carboxylate groups in the side chain modifies the charge of the ligands and the complexes. We have investigated the copper(II) and nickel(II) complexes of di-, tri- and tetra-peptides containing one, two or more aspartic an ...
... formation processes has already been widely studied. However the presence of carboxylate groups in the side chain modifies the charge of the ligands and the complexes. We have investigated the copper(II) and nickel(II) complexes of di-, tri- and tetra-peptides containing one, two or more aspartic an ...
Oxidation of benzoin with anchored vanadyl and
... gave lesser yield for benzil and the methyl ester and acid products were also formed. Thus for the oxidation of benzoin to benzil, the anchored MoO,(salphen) complex catalyst was observed to be more effective. It is evident from the results in Table 2 that the best catalyst for the oxidation of benz ...
... gave lesser yield for benzil and the methyl ester and acid products were also formed. Thus for the oxidation of benzoin to benzil, the anchored MoO,(salphen) complex catalyst was observed to be more effective. It is evident from the results in Table 2 that the best catalyst for the oxidation of benz ...
PDF - School of Chemical Sciences
... NAD(P)H regeneration is important for biocatalytic reactions that require these costly cofactors. A mutant phosphite dehydrogenase (PTDHE175A ⁄ A176R) that utilizes both NAD and NADP efficiently is a very promising system for NAD(P)H regeneration. In this work, both the kinetic mechanism and practica ...
... NAD(P)H regeneration is important for biocatalytic reactions that require these costly cofactors. A mutant phosphite dehydrogenase (PTDHE175A ⁄ A176R) that utilizes both NAD and NADP efficiently is a very promising system for NAD(P)H regeneration. In this work, both the kinetic mechanism and practica ...
Evolution of the Insulin Receptor Family and
... among its members. Phylogenetic analyses were based on an amino acid sequence data alignment including 1,596 positions, of which 335 highly variable positions were excluded due to ambiguity in positional homology assignment. A total of 250 positions were invariant, and 813 characters were parsimony ...
... among its members. Phylogenetic analyses were based on an amino acid sequence data alignment including 1,596 positions, of which 335 highly variable positions were excluded due to ambiguity in positional homology assignment. A total of 250 positions were invariant, and 813 characters were parsimony ...
Two-Electron Reduction of a Vanadium(V) Nitride by CO to Release
... discrete metal complexes bearing oxo ligands have been shown to provide CO2 upon treatment with CO,17 and heterogeneous catalysis provides many examples of CO acting as a terminal reducing agent on metal oxide materials to produce CO2.18 In addition, the newly observed reactivity may shed light on t ...
... discrete metal complexes bearing oxo ligands have been shown to provide CO2 upon treatment with CO,17 and heterogeneous catalysis provides many examples of CO acting as a terminal reducing agent on metal oxide materials to produce CO2.18 In addition, the newly observed reactivity may shed light on t ...
Docking of B-cell epitope antigen to specific hepatitis B antibody
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
Effects of mutation on key amino acid residues in
... identified in each of the subunits. The N-terminal region (amino acid residues 1-42) is responsible for interactions with transcription factors, binding to MDM2 and transactivation activity [1]. Amino acid residues 17 – 29 in this region form an alpha-helix which binds to the hydrophobic cleft in MD ...
... identified in each of the subunits. The N-terminal region (amino acid residues 1-42) is responsible for interactions with transcription factors, binding to MDM2 and transactivation activity [1]. Amino acid residues 17 – 29 in this region form an alpha-helix which binds to the hydrophobic cleft in MD ...
A chemogenomic analysis of the transmembrane binding cavity of
... view of a putative ligand. To achieve this task, 30 critical amino acids disseminated on the 7-TM domain were extracted for each receptor (Fig. 1). Our clustering approach implies two assumptions: (1) the overall fold of the 7-TM domain around the binding crevice has been conserved along evolution; ...
... view of a putative ligand. To achieve this task, 30 critical amino acids disseminated on the 7-TM domain were extracted for each receptor (Fig. 1). Our clustering approach implies two assumptions: (1) the overall fold of the 7-TM domain around the binding crevice has been conserved along evolution; ...
IOSR Journal of Applied Chemistry (IOSR-JAC) e-ISSN: 2278-5736.
... activity has been challenging for bioinorganic chemists and recently some complexes with high catalytic activity have been reported [1-3]. Nikel-contaning superoxide dismutase (NiSOD) has been isolated from several streptomyces species [4,5].The enzymatic activity of NiSOD [6], is as high has that o ...
... activity has been challenging for bioinorganic chemists and recently some complexes with high catalytic activity have been reported [1-3]. Nikel-contaning superoxide dismutase (NiSOD) has been isolated from several streptomyces species [4,5].The enzymatic activity of NiSOD [6], is as high has that o ...
Isolation of drug target protein
... MTX (Methotrexate) is an anti metabolite and anti folate drug. It is used in treatment of cancer and autoimmune diseases. We immobilized MTX derivatives on FG beads to capture target molecules in HeLa cell extracts. MTX derivatives Result 1. FG beads captured DFHR which is an enzyme that reduces dih ...
... MTX (Methotrexate) is an anti metabolite and anti folate drug. It is used in treatment of cancer and autoimmune diseases. We immobilized MTX derivatives on FG beads to capture target molecules in HeLa cell extracts. MTX derivatives Result 1. FG beads captured DFHR which is an enzyme that reduces dih ...