SUPPLEMENTARY INFORMATION
... Supplementary Fig. 5. Effects of various salts on the thermostability of purified vcINDY ...
... Supplementary Fig. 5. Effects of various salts on the thermostability of purified vcINDY ...
12659942_three sites - University of Canterbury
... amide group of Asn175, a water molecule, which in turn interacts with the indole N–H of Trp3, and a salt bridge to the guanidinium moiety of Arg171. The amino group of Tyr forms hydrogen bonds to the backbone carbonyl of Phe91, the side-chain carbonyl of Asn175 and, mediated through a water molecule ...
... amide group of Asn175, a water molecule, which in turn interacts with the indole N–H of Trp3, and a salt bridge to the guanidinium moiety of Arg171. The amino group of Tyr forms hydrogen bonds to the backbone carbonyl of Phe91, the side-chain carbonyl of Asn175 and, mediated through a water molecule ...
Calcitonin Gene-Related Peptides
... have been suggested. Due to its potent vasodilatory action and its ionotropic and chronotropic effects, CGRP is likely to play a role in cardiovascular homeostasis. Furthermore, it influences feeding and digestion since it has shown to decrease food intake, gastric secretion, and intestinal motility ...
... have been suggested. Due to its potent vasodilatory action and its ionotropic and chronotropic effects, CGRP is likely to play a role in cardiovascular homeostasis. Furthermore, it influences feeding and digestion since it has shown to decrease food intake, gastric secretion, and intestinal motility ...
PDF File
... the observed K1/2 values equal the dissociation constants Kd (see also ref 37): The same K1/2 values were observed in concentration dependences in which the maximal rate constant for reaction varied by more than 10-fold, which was accomplished by a 2′-H substitution at position -1 and by varying the ...
... the observed K1/2 values equal the dissociation constants Kd (see also ref 37): The same K1/2 values were observed in concentration dependences in which the maximal rate constant for reaction varied by more than 10-fold, which was accomplished by a 2′-H substitution at position -1 and by varying the ...
Escherichia coli ATP Synthase
... cancer, heart disease, mitochondrial diseases, immune deficiency, cystic fibrosis, diabetes, ulcers, and tuberculosis that affect both human and animals have been associated with ATP synthase ([1, 17] and references therein). The presence of ATP synthase on the surfaces of multiple cell types, and it ...
... cancer, heart disease, mitochondrial diseases, immune deficiency, cystic fibrosis, diabetes, ulcers, and tuberculosis that affect both human and animals have been associated with ATP synthase ([1, 17] and references therein). The presence of ATP synthase on the surfaces of multiple cell types, and it ...
Coenzyme B 12-Dependent Ribonucleotide Reductase: Evidence
... Natl. Acad. Sci. U.S.A.90,8352-83561 have now allowed its characterization by site-directed mutagenesis. The present study focuses on the role of five cysteines postulated to be required for catalysis. The choice of which of the ten cysteines of RTPR were to be mutated was based on extensive studies ...
... Natl. Acad. Sci. U.S.A.90,8352-83561 have now allowed its characterization by site-directed mutagenesis. The present study focuses on the role of five cysteines postulated to be required for catalysis. The choice of which of the ten cysteines of RTPR were to be mutated was based on extensive studies ...
Molecular and Functional Characterization of Human P2X2 Receptors
... Cloning of the Rat P2X2 and Human P2X3 Receptors. The rat P2X2 and P2X2b receptor cDNAs were isolated through RT-PCR reactions from oligo(dT)-primed cDNA synthesized from total brain polyA1 RNA (Clontech). Primers were designed based on the published sequence for the rat P2X2 receptors (GenBank acce ...
... Cloning of the Rat P2X2 and Human P2X3 Receptors. The rat P2X2 and P2X2b receptor cDNAs were isolated through RT-PCR reactions from oligo(dT)-primed cDNA synthesized from total brain polyA1 RNA (Clontech). Primers were designed based on the published sequence for the rat P2X2 receptors (GenBank acce ...
Coordination and Chemistry of Stable Cu (II) Complexes in the Gas
... even number. Results and Discussion Experiments have been undertaken on the solvation of Cu(II) in association with the 20 ligands listed in Table 1. Also listed are several physical properties of the ligands that could have an influence on the stability of a given complex. In two instances it was n ...
... even number. Results and Discussion Experiments have been undertaken on the solvation of Cu(II) in association with the 20 ligands listed in Table 1. Also listed are several physical properties of the ligands that could have an influence on the stability of a given complex. In two instances it was n ...
Induction of y-Gultamyl Transpeptidase by a Thyroid Hormone in
... neutralization by ammonia production (Tate, 1980). Therefore, the induction of GGT activity may represent a necessary physiological adaptation in the cell differentiation, growth, and the increase of metabolic rates by upregulating the synthesis of cellular glutathione. Recently, it was found that t ...
... neutralization by ammonia production (Tate, 1980). Therefore, the induction of GGT activity may represent a necessary physiological adaptation in the cell differentiation, growth, and the increase of metabolic rates by upregulating the synthesis of cellular glutathione. Recently, it was found that t ...
A new natural product-based hybrid
... able to target several nodes in a disease-causing network is considered to be highly beneficial for treating complex diseases (5, 6). Many specific lead generation strategies have been used earlier for the rational design of inhibitors with predefined multi-target profile, of which most ...
... able to target several nodes in a disease-causing network is considered to be highly beneficial for treating complex diseases (5, 6). Many specific lead generation strategies have been used earlier for the rational design of inhibitors with predefined multi-target profile, of which most ...
Patrick Tb Ch04
... 22) Which statement best describes the relevance of an allosteric binding site to medicinal chemistry? Feedback: Designing drugs to interact with allosteric binding site allows a method of regulating enzyme activity, other than using inhibitors which bind to the active site. Allosteric binding sites ...
... 22) Which statement best describes the relevance of an allosteric binding site to medicinal chemistry? Feedback: Designing drugs to interact with allosteric binding site allows a method of regulating enzyme activity, other than using inhibitors which bind to the active site. Allosteric binding sites ...
Activin Receptor IA human (A0699) - Datasheet - Sigma
... IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been ...
... IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been ...
PhoR, PhoP and MshC: Three essential proteins of Mycobacterium
... of PhoRin and PhoP, a pull-down method was developed using the non-hydrolyzable ATP analog, adenosine 5′-(β,γ-imido)triphosphate (AMP-PNP). A stable PhoRin-AMPPNP-PhoP complex was not identified. Future work is necessary for the crystallization of PhoRex and PhoRin. A new DNA binding assay is also n ...
... of PhoRin and PhoP, a pull-down method was developed using the non-hydrolyzable ATP analog, adenosine 5′-(β,γ-imido)triphosphate (AMP-PNP). A stable PhoRin-AMPPNP-PhoP complex was not identified. Future work is necessary for the crystallization of PhoRex and PhoRin. A new DNA binding assay is also n ...
chapter 23 the transition elements and their
... Name nickel as nickel(II) to indicate oxidation state. Ligands are six (hexa-) waters (aqua). Put together with chloride anions to give hexaaquanickel(II) chloride. b) The cation is [Cr(en)3]n+ and the anion is ClO4–, the perchlorate ion. The charge on the cation is +3 to make a neutral salt in comb ...
... Name nickel as nickel(II) to indicate oxidation state. Ligands are six (hexa-) waters (aqua). Put together with chloride anions to give hexaaquanickel(II) chloride. b) The cation is [Cr(en)3]n+ and the anion is ClO4–, the perchlorate ion. The charge on the cation is +3 to make a neutral salt in comb ...
Inquiry into Life Twelfth Edition
... Eukaryotic initiation factors and general functions: • eIF2 binds Met-tRNA to ribosomes • eIF2B activates eIF2 replacing its GDP with GTP • eIF1 and eIF1A aid in scanning to initiation codon • eIF3 binds to 40S ribosomal subunit, inhibits reassociation with 60S subunit • eIF4 is a cap-binding protei ...
... Eukaryotic initiation factors and general functions: • eIF2 binds Met-tRNA to ribosomes • eIF2B activates eIF2 replacing its GDP with GTP • eIF1 and eIF1A aid in scanning to initiation codon • eIF3 binds to 40S ribosomal subunit, inhibits reassociation with 60S subunit • eIF4 is a cap-binding protei ...
Thyroid Hormones_MJH_Class of 2016
... thyroid hormones • Glycerol 3-phosphate dehydrogenase – main component of glycerol 3-phosphate shuttle in mitochondria (one of transport systems for NADH into mitochondria) • Cytochrome c oxidase – the complex mitochondrial enzyme in the electron transport chain (from cytochrome c to oxygen) • ATPas ...
... thyroid hormones • Glycerol 3-phosphate dehydrogenase – main component of glycerol 3-phosphate shuttle in mitochondria (one of transport systems for NADH into mitochondria) • Cytochrome c oxidase – the complex mitochondrial enzyme in the electron transport chain (from cytochrome c to oxygen) • ATPas ...
A first attempt to elucidate the amino acid sequence of some lichen
... to form a new biological entity different from its individual components. Recognition mechanisms used by lichens are based on the production and secretion of fungal lectins as signalling molecules (Kardish et al., 1991), which therefore develop arginase enzymatic activity (Molina et al., 1993). Wher ...
... to form a new biological entity different from its individual components. Recognition mechanisms used by lichens are based on the production and secretion of fungal lectins as signalling molecules (Kardish et al., 1991), which therefore develop arginase enzymatic activity (Molina et al., 1993). Wher ...
Pivotal Role of Water in the Mechanism of
... good model system for class II P450s and P450BM-3. Most notably, P450cam undergoes only a slight structural rearrangement upon reduction of the substrate-bound form, whereas P450BM-3 has been shown to require a much more substantial structural rearrangement of the substrate and, by implication, the ...
... good model system for class II P450s and P450BM-3. Most notably, P450cam undergoes only a slight structural rearrangement upon reduction of the substrate-bound form, whereas P450BM-3 has been shown to require a much more substantial structural rearrangement of the substrate and, by implication, the ...
Kinetic mechanism of the dimeric ATP sulfurylase from plants
... the same buffer. Protein and ligand solutions were degassed at room temperature (22 ◦ C) prior to use. For each titration, 20–30 injections of 10 μl of ligand were added into sample solutions containing protein (18.5–50 μM) in the presence or absence of ligands. GmATPS1 complexed with APS was formed ...
... the same buffer. Protein and ligand solutions were degassed at room temperature (22 ◦ C) prior to use. For each titration, 20–30 injections of 10 μl of ligand were added into sample solutions containing protein (18.5–50 μM) in the presence or absence of ligands. GmATPS1 complexed with APS was formed ...
Corticotropin-Releasing Factor Receptor Antagonists: Potential
... adenosine 3’-5’-cyclic monophosphate (cAMP; Prod. No. A 9501). In vitro, guanine nucleotides have been shown to decrease the specific binding of agonists for a large number of seven transmembrane neurohormone or neurotransmitter receptors coupled to G proteins, presumably by uncoupling the G protein ...
... adenosine 3’-5’-cyclic monophosphate (cAMP; Prod. No. A 9501). In vitro, guanine nucleotides have been shown to decrease the specific binding of agonists for a large number of seven transmembrane neurohormone or neurotransmitter receptors coupled to G proteins, presumably by uncoupling the G protein ...
Coordination Chemistry: Bonding
... NPTEL – Chemistry and Biochemistry – Coordination Chemistry (Chemistry of transition elements) ...
... NPTEL – Chemistry and Biochemistry – Coordination Chemistry (Chemistry of transition elements) ...
Fluorescence Enhancement of Fluorescein Isothiocyanate
... caused by the dispersion of FITC-Protein A-immobilization in each sample glass and plasma-related noise for fluorescence. More sensitive measurement will be achieved by the further improvement in immobilization method and choice of fluorescence dye, because fluorescence wavelength of FITC is overlap ...
... caused by the dispersion of FITC-Protein A-immobilization in each sample glass and plasma-related noise for fluorescence. More sensitive measurement will be achieved by the further improvement in immobilization method and choice of fluorescence dye, because fluorescence wavelength of FITC is overlap ...
HYBRID MULTIDENTATE PHOSPHINE
... metals (Pt, Pd, Cu, Rh and Au) has been investigated in a comprehensive spectroscopis study. Single crystal X-ray analysis has been conducted at suitable junctures within the project. A surprising finding was that some solution-state structures were found to be different when studied in the solid st ...
... metals (Pt, Pd, Cu, Rh and Au) has been investigated in a comprehensive spectroscopis study. Single crystal X-ray analysis has been conducted at suitable junctures within the project. A surprising finding was that some solution-state structures were found to be different when studied in the solid st ...
Chapter One Hemilabile Ligands in Transition
... which is an ether-phosphine ligand. Phosphines are one of the most versatile ligands that can bind to late transition metals, since they produce stable metal-phosphorus bonds.9 Hemilabile phosphines have potentially important industrial applications through the ability to tune the properties of the ...
... which is an ether-phosphine ligand. Phosphines are one of the most versatile ligands that can bind to late transition metals, since they produce stable metal-phosphorus bonds.9 Hemilabile phosphines have potentially important industrial applications through the ability to tune the properties of the ...
Characterization of the binding properties of the Avian Coronavirus
... Protein biochemistry ................................................................................. 47 ...
... Protein biochemistry ................................................................................. 47 ...