Biological significance of structural differences between two highly
... (Fig. 1A), because it lies in proximity to a.a. 116–129 in the Uev1 molecular structure (Fig. 1C). The Uev1D30-F104S single amino acid substitution abrogated binding by LN2A, but did not affect ...
... (Fig. 1A), because it lies in proximity to a.a. 116–129 in the Uev1 molecular structure (Fig. 1C). The Uev1D30-F104S single amino acid substitution abrogated binding by LN2A, but did not affect ...
Chapter 6 - IFSC-USP
... are separate molecules. The former are called ionotropic receptors or ligandgated ion channels, and give rise to fast postsynaptic responses that typically last only a few milliseconds. The latter are called metabotropic receptors, and they produce slower postsynaptic effects that may endure much lo ...
... are separate molecules. The former are called ionotropic receptors or ligandgated ion channels, and give rise to fast postsynaptic responses that typically last only a few milliseconds. The latter are called metabotropic receptors, and they produce slower postsynaptic effects that may endure much lo ...
9. steroid hormones - cmb
... cholesterol, and is inactivated when imported into the mitochondria • This process is important in the regulation of steroidogenesis. ...
... cholesterol, and is inactivated when imported into the mitochondria • This process is important in the regulation of steroidogenesis. ...
Structural Features of Angiotensin-I Converting Enzyme Catalytic Sites
... amino acids between the first two ligands. On the other hand, the long spacers usually found in various metallopeptidase subfamilies could vary from 5 to over 100 amino acids. The length of the spacer between the two first ligands belonging to the same binding motif often characterizes the secondary ...
... amino acids between the first two ligands. On the other hand, the long spacers usually found in various metallopeptidase subfamilies could vary from 5 to over 100 amino acids. The length of the spacer between the two first ligands belonging to the same binding motif often characterizes the secondary ...
The reference range for serum creatinine is as follows
... The assay of creatinine has been based on the reaction of creatinine with alkaline picrate. The reaction has been performed on a protein free filtrate. It has long been recognized that the red color formed in this reaction is not absolutely specific for creatinine, thus results obtained are too high ...
... The assay of creatinine has been based on the reaction of creatinine with alkaline picrate. The reaction has been performed on a protein free filtrate. It has long been recognized that the red color formed in this reaction is not absolutely specific for creatinine, thus results obtained are too high ...
Transition Metals
... It could also be done by use of a spectrometer measuring the intensity of the purple colour. This method has the advantage that it does not disrupt the reaction mixture, using up the reactants and it leads to a much quicker determination of concentration ...
... It could also be done by use of a spectrometer measuring the intensity of the purple colour. This method has the advantage that it does not disrupt the reaction mixture, using up the reactants and it leads to a much quicker determination of concentration ...
RNA–Amino Acid Binding - University of Colorado-MCDB
... such interactions are probable, independent of mooring of the amino acid to the RNA site via an adenosine residue, as occurs in SAM aptamer structures (e.g., in Fig. 1 above). Of course, even the aliphatic sections of side chains can interact with nucleobases by van der Waals and hydrophobic (entrop ...
... such interactions are probable, independent of mooring of the amino acid to the RNA site via an adenosine residue, as occurs in SAM aptamer structures (e.g., in Fig. 1 above). Of course, even the aliphatic sections of side chains can interact with nucleobases by van der Waals and hydrophobic (entrop ...
Crystal structure of mouse coronavirus receptor
... fusion protein (17). During maturation, the spike protein is often cleaved into a receptor-binding subunit S1 and a membranefusion subunit S2 that associate together through noncovalent interactions (Fig. 1A). S1 sequences are relatively well conserved within each coronavirus group, but differ marke ...
... fusion protein (17). During maturation, the spike protein is often cleaved into a receptor-binding subunit S1 and a membranefusion subunit S2 that associate together through noncovalent interactions (Fig. 1A). S1 sequences are relatively well conserved within each coronavirus group, but differ marke ...
A Point Mutation in Transthyretin Increases Affinity for Thyroxine and
... oligonucleotide primer contained a PST- 1 restriction enzyme recognition site that was used for subsequent subcloning (15). PCR product DNA fragments were visualized after agarose gel electrophoresis (1.8% agarose) in the presence of ethidium bromide. Appropriate length fragments were excised from t ...
... oligonucleotide primer contained a PST- 1 restriction enzyme recognition site that was used for subsequent subcloning (15). PCR product DNA fragments were visualized after agarose gel electrophoresis (1.8% agarose) in the presence of ethidium bromide. Appropriate length fragments were excised from t ...
Distribution bias analysis of germline and somatic single
... cancer associated gene lists. For illustrating how key pfsSNVs can be prioritized further, we provide a use case where we performed survival analysis showing that a loss of phosphorylation site pfsSNV at position 105 in MEF2A is significantly associated with decreased pancreatic cancer patient survi ...
... cancer associated gene lists. For illustrating how key pfsSNVs can be prioritized further, we provide a use case where we performed survival analysis showing that a loss of phosphorylation site pfsSNV at position 105 in MEF2A is significantly associated with decreased pancreatic cancer patient survi ...
Efficient Phosphodiester Hydrolysis by
... model substrate BDNPP were monitored spectrophotometrically for the absorbance increase at 400 nm due to the formation of 2,4-dinitrophenolate over time, under conditions of excess complex at 25 °C, and the reaction mixtures were monitored until saturation was reached. In these experiments, all of t ...
... model substrate BDNPP were monitored spectrophotometrically for the absorbance increase at 400 nm due to the formation of 2,4-dinitrophenolate over time, under conditions of excess complex at 25 °C, and the reaction mixtures were monitored until saturation was reached. In these experiments, all of t ...
The Anaerobic (Class III) Ribonucleotide Reductase from Lactococcus lactis
... were designed to generate NdeI and XhoI restriction sites, respectively, at the start and the end of the PCR fragment. The amplified product (630 bp) was purified and cloned into plasmid pGEM-T (Promega), giving plasmid pIG021. After digestion with NdeI and XhoI, the nrdG fragment was ligated into p ...
... were designed to generate NdeI and XhoI restriction sites, respectively, at the start and the end of the PCR fragment. The amplified product (630 bp) was purified and cloned into plasmid pGEM-T (Promega), giving plasmid pIG021. After digestion with NdeI and XhoI, the nrdG fragment was ligated into p ...
Binding of Straight-Chain Saturated Dicarboxylic Acids to Albumin
... was from Sigma Chemical Co. and further purified as described by Spector, John, and Fletcher ( 18). The monocarboxylic fatty acid content of the purified albumin was determined by gas-liquid chromatography (4) and found to have < 0.02 mol fatty acid/mol of albumin. Equilibrium dialysis. Binding of d ...
... was from Sigma Chemical Co. and further purified as described by Spector, John, and Fletcher ( 18). The monocarboxylic fatty acid content of the purified albumin was determined by gas-liquid chromatography (4) and found to have < 0.02 mol fatty acid/mol of albumin. Equilibrium dialysis. Binding of d ...
Macrocyclic Carbohydrate/Amino Acid Hybrid Molecules
... 1.2 Design of artificial receptors Proteins bind other molecules through a combination of electrostatic interactions, such as ion pairing and hydrogen bonding, and hydrophobic effects, i.e. the association of hydrophobic regions in a polar solvent.24 These non-covalent interactions are generally not ...
... 1.2 Design of artificial receptors Proteins bind other molecules through a combination of electrostatic interactions, such as ion pairing and hydrogen bonding, and hydrophobic effects, i.e. the association of hydrophobic regions in a polar solvent.24 These non-covalent interactions are generally not ...
Towards the Molecular Basis of Sperm and Egg Interaction during
... transfected EC-mZP3 cell lines. pPGK-1 represents the mouse phosphoglycerate kinase1 promoter region. Restriction enzymes ClaI and SstII were used to generate linearized DNA fragments for electroporation of EC (F9) cells. Arrow indicates the transcriptional start site on the PGK-1 promoter. The site ...
... transfected EC-mZP3 cell lines. pPGK-1 represents the mouse phosphoglycerate kinase1 promoter region. Restriction enzymes ClaI and SstII were used to generate linearized DNA fragments for electroporation of EC (F9) cells. Arrow indicates the transcriptional start site on the PGK-1 promoter. The site ...
Lecture 12 Enzymes: Inhibition
... – covalently (irreversibly) inactivates enzyme (PGH2 synthase, cyclooxygenase activity, also known in its two forms as COX 1 and COX 2) involved in prostaglandin biosynthesis – anti-inflammatory action due to blocking of prostaglandin synthesis – covalently modifies (acetylates) specific Ser-OH grou ...
... – covalently (irreversibly) inactivates enzyme (PGH2 synthase, cyclooxygenase activity, also known in its two forms as COX 1 and COX 2) involved in prostaglandin biosynthesis – anti-inflammatory action due to blocking of prostaglandin synthesis – covalently modifies (acetylates) specific Ser-OH grou ...
The Genetic Code and RNA-Amino Acid Affinities
... contiguous sequences, usually having ≤24 essential nucleotides, should be the most likely to be isolated. These 24 “essential nucleotides” are defined by statistics. If only purines occur at a given position, this is twice as likely to occur as one specific nucleotide. In this case, selection can is ...
... contiguous sequences, usually having ≤24 essential nucleotides, should be the most likely to be isolated. These 24 “essential nucleotides” are defined by statistics. If only purines occur at a given position, this is twice as likely to occur as one specific nucleotide. In this case, selection can is ...
Engineering of cyclodextrin glycosyltransferase reaction and
... need for a solvent recovery process [15]. The availability of K- and Q-cyclodextrins is thus rather limited at present; consequently, there is a great demand for a process that could produce these cyclodextrins economically. Also the processes used for L-cyclodextrin production are not ideal, since ...
... need for a solvent recovery process [15]. The availability of K- and Q-cyclodextrins is thus rather limited at present; consequently, there is a great demand for a process that could produce these cyclodextrins economically. Also the processes used for L-cyclodextrin production are not ideal, since ...
Reaction Mechanisms of Mononuclear Non
... of structural simulations. We present herein a comprehensive comparison of the available structures for the three AAAHs using data produced over the past 6 years. The data summarized here have shed some light on the interactions between the iron-cofactor, iron-substrate, and the stabilization of the ...
... of structural simulations. We present herein a comprehensive comparison of the available structures for the three AAAHs using data produced over the past 6 years. The data summarized here have shed some light on the interactions between the iron-cofactor, iron-substrate, and the stabilization of the ...
Biochemistry 499
... Sepharose resin. From the binding capacity experiments, 10mg of microcystin-LR can bind 42 mg of PP1, which would make large-scale purification of PP1 possible. The reactions involved in creating the resin, microcystin-LR with aminoethanethiol and the product of that reaction with NHS-activated Seph ...
... Sepharose resin. From the binding capacity experiments, 10mg of microcystin-LR can bind 42 mg of PP1, which would make large-scale purification of PP1 possible. The reactions involved in creating the resin, microcystin-LR with aminoethanethiol and the product of that reaction with NHS-activated Seph ...
X-ray crystal structure of plasmin with tranexamic
... dimension of Plg in solution (Dmax) (Figure 4). The SAXS data revealed that in the presence of YO inhibitors, full-length Plg remained in the closed form, suggesting that the YO inhibitors are unlikely to interact with the LBSs of Plg or Plm. Finally, we determined the crystal structure of a second ...
... dimension of Plg in solution (Dmax) (Figure 4). The SAXS data revealed that in the presence of YO inhibitors, full-length Plg remained in the closed form, suggesting that the YO inhibitors are unlikely to interact with the LBSs of Plg or Plm. Finally, we determined the crystal structure of a second ...
Coordination Chemistry Reviews Iron–dinitrogen coordination
... There are three types of nitrogenase enzymes, which vary by the metal composition in the active site: iron and molybdenum, iron and vanadium, and iron only [17]. The three nitrogenases all have similar structures and reactivity, with the latter two types typically produced only under molybdenum defic ...
... There are three types of nitrogenase enzymes, which vary by the metal composition in the active site: iron and molybdenum, iron and vanadium, and iron only [17]. The three nitrogenases all have similar structures and reactivity, with the latter two types typically produced only under molybdenum defic ...
Structure of L‑Serine Dehydratase from Legionella
... column using standard procedures.14,21 Mutant proteins were prepared by PCR as previously described22 and purified in the same manner that was used for the wild-type enzyme. Kinetic Analysis. Activity was measured by following the absorbance of product (pyruvate) formation at 250 nm.14 The concentrat ...
... column using standard procedures.14,21 Mutant proteins were prepared by PCR as previously described22 and purified in the same manner that was used for the wild-type enzyme. Kinetic Analysis. Activity was measured by following the absorbance of product (pyruvate) formation at 250 nm.14 The concentrat ...
Caspaar Bijleveld and Math JH Geelen
... fatty acid synthase complex - present in the cytosol - will also catalyse the decarboxylation of malonyl-CoA both in the presence and absence of NADPH [lO,ll]. The accumulation of malonylCoA in the bicarbonate-fixation assay allows a significant decarboxylation of this reaction product. Fourthly, ac ...
... fatty acid synthase complex - present in the cytosol - will also catalyse the decarboxylation of malonyl-CoA both in the presence and absence of NADPH [lO,ll]. The accumulation of malonylCoA in the bicarbonate-fixation assay allows a significant decarboxylation of this reaction product. Fourthly, ac ...
Identification of the Human Erythrocyte Glucose Transporter (GLUT1
... Follow this and additional works at: http://escholarship.umassmed.edu/gsbs_diss Part of the Life Sciences Commons, and the Medicine and Health Sciences Commons ...
... Follow this and additional works at: http://escholarship.umassmed.edu/gsbs_diss Part of the Life Sciences Commons, and the Medicine and Health Sciences Commons ...