E. Coli MurG: A Paradigm for a Superfamily of
... MurG contains two domains separated by a cleft that is approximately 20Å deep × 18Å wide at the widest point. Both domains adopt an α/β open-sheet motif, also known as a Rossmann fold. A common Rossmann motif contains six β strands connected by α helices. The β strands form a twisted parallel sheet ...
... MurG contains two domains separated by a cleft that is approximately 20Å deep × 18Å wide at the widest point. Both domains adopt an α/β open-sheet motif, also known as a Rossmann fold. A common Rossmann motif contains six β strands connected by α helices. The β strands form a twisted parallel sheet ...
Structure of a Pheromone Receptor-Associated MHC Molecule with
... Rich Olson1, Kathryn E. Huey-Tubman1,2, Catherine Dulac3, Pamela J. Bjorkman1,2* 1 Division of Biology, California Institute of Technology, Pasadena, California, United States of America, 2 Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, United States of Am ...
... Rich Olson1, Kathryn E. Huey-Tubman1,2, Catherine Dulac3, Pamela J. Bjorkman1,2* 1 Division of Biology, California Institute of Technology, Pasadena, California, United States of America, 2 Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, United States of Am ...
Spectroscopic Comparisons of the pH Dependencies of Fe
... where M signifies the bound metal ion. Although Fe- and Mn-specific SODs are very closely related to one another, they are essentially unrelated to the Cu and Zn-containing SODs. Fe- and Mn-SODs are dimers or tetramers of identical monomers, which each have one active site containing a single Fe or ...
... where M signifies the bound metal ion. Although Fe- and Mn-specific SODs are very closely related to one another, they are essentially unrelated to the Cu and Zn-containing SODs. Fe- and Mn-SODs are dimers or tetramers of identical monomers, which each have one active site containing a single Fe or ...
Unusual properties of the fungal conventional kinesin neck domain
... conditions (pH 3), the circular dichroism (CD) spectrum resembled those usually reported for two-stranded a-helical coiled-coils ([Q]222/[Q]208 = 1.03). This observation suggests an important role for the ionized glutamic acid residues for the onset of this type of fold. Moreover, at pH 3, the molar ...
... conditions (pH 3), the circular dichroism (CD) spectrum resembled those usually reported for two-stranded a-helical coiled-coils ([Q]222/[Q]208 = 1.03). This observation suggests an important role for the ionized glutamic acid residues for the onset of this type of fold. Moreover, at pH 3, the molar ...
Studies Into the Allosteric Regulation of ADP
... In glycogen, chains of α-1,4-polyglucans link with other chains to form a branching pattern. In mammals and yeast, glycogen synthesis requires a 'seed' of glycogenin, which is a self-glucosylating initiator protein, although no analogue of glycogen has been described in bacteria.4 Glycogen is a larg ...
... In glycogen, chains of α-1,4-polyglucans link with other chains to form a branching pattern. In mammals and yeast, glycogen synthesis requires a 'seed' of glycogenin, which is a self-glucosylating initiator protein, although no analogue of glycogen has been described in bacteria.4 Glycogen is a larg ...
Sam P. de Visser,* Jan-Uwe Rohde,* Yong
... 2His/1carboxylate ligand motif that links the metal to the protein. The catalytic cycle of one representative enzyme, taurine:˛ketoglutarate dioxygenase (TauD) [7,28–34], is shown in Fig. 2 and starts from a resting state where the three remaining Fe coordination sites are occupied by water molecule ...
... 2His/1carboxylate ligand motif that links the metal to the protein. The catalytic cycle of one representative enzyme, taurine:˛ketoglutarate dioxygenase (TauD) [7,28–34], is shown in Fig. 2 and starts from a resting state where the three remaining Fe coordination sites are occupied by water molecule ...
couverture these PRES Toulouse M ESCARCEGA 2011
... through this cycle before becoming inactivated is called the turnover number (TON) and is a measure of the catalyst stability. The turnover frequency (TOF) is the number of moles of product per mole of catalyst per unit time or the TON in a certain period and is a measure of the catalyst efficiency. ...
... through this cycle before becoming inactivated is called the turnover number (TON) and is a measure of the catalyst stability. The turnover frequency (TOF) is the number of moles of product per mole of catalyst per unit time or the TON in a certain period and is a measure of the catalyst efficiency. ...
strain UNS 35 - Journal of Medical Microbiology
... DEGRADATION OF HYALURONATE BY S. INTERMEDIUS ...
... DEGRADATION OF HYALURONATE BY S. INTERMEDIUS ...
Localization and nucleotide specificity of Blastocystis succinyl‐CoA
... production via succinyl-CoA synthetase (SCS), an enzyme that is also found in the mitochondrial TCA cycle (Dacks et al., 2006). Succinyl-CoA synthetase is composed of two subunits, alpha and beta, which generally form dimers in eukaryotes and tetramers in prokaryotes (Bridger et al., 1987). The subs ...
... production via succinyl-CoA synthetase (SCS), an enzyme that is also found in the mitochondrial TCA cycle (Dacks et al., 2006). Succinyl-CoA synthetase is composed of two subunits, alpha and beta, which generally form dimers in eukaryotes and tetramers in prokaryotes (Bridger et al., 1987). The subs ...
A monoclonal antibody against a carbohydrate
... The genes encoding the variable domains of mAb S69-4 were cloned and expressed as single-chain variable fragments (scFv) to allow a sequence comparison with the two well characterized antibodies mAb S45-18 and mAb S25-2 which have been crystallized in complex with the antigen (Nguyen et al., 2003). ...
... The genes encoding the variable domains of mAb S69-4 were cloned and expressed as single-chain variable fragments (scFv) to allow a sequence comparison with the two well characterized antibodies mAb S45-18 and mAb S25-2 which have been crystallized in complex with the antigen (Nguyen et al., 2003). ...
Further studies on the new coomassie brilliant blue G-250 - K-REx
... sensitive than the biuret, requires no digestion of protein and can be completed in about 40 minutes. Finally, an assay which is widely used and depends upon dye binding was introduced by Udy (1954) ...
... sensitive than the biuret, requires no digestion of protein and can be completed in about 40 minutes. Finally, an assay which is widely used and depends upon dye binding was introduced by Udy (1954) ...
Sodium-Coupled Neurotransmitter Transporters Baruch I. Kanner* and Elia Zomot
... extracellular dopamine is about 100 times longer than normal.5 The study of glutamate transporter knockout mice indicates that glutamate transporters, in particular GLT-1,6 play a central role in preventing both hyperexcitability and excitotoxicity.7 The transporters are slow, with turnover rates ra ...
... extracellular dopamine is about 100 times longer than normal.5 The study of glutamate transporter knockout mice indicates that glutamate transporters, in particular GLT-1,6 play a central role in preventing both hyperexcitability and excitotoxicity.7 The transporters are slow, with turnover rates ra ...
Engineering subunit association of multisubunit proteins
... analysis of complexes, assumes, as a first-order approximation, rigid body association. The same binding free energy evaluation model could also be used to address the third problem for further design of dimeric streptavidins, in which the biotinbinding affinity, reduced due to the subunit separatio ...
... analysis of complexes, assumes, as a first-order approximation, rigid body association. The same binding free energy evaluation model could also be used to address the third problem for further design of dimeric streptavidins, in which the biotinbinding affinity, reduced due to the subunit separatio ...
Molecular Modeling of Substrate Binding in Wild
... genetically modified bacteria expressing the gene for 2,5 diketo-D-gluconic acid reductase.1,2 These genetic manipulations have resulted in bacteria that can directly produce 2-KLG from D-glucose. In principle, this method may have considerable advantage over the currently used Reichstein and Grussn ...
... genetically modified bacteria expressing the gene for 2,5 diketo-D-gluconic acid reductase.1,2 These genetic manipulations have resulted in bacteria that can directly produce 2-KLG from D-glucose. In principle, this method may have considerable advantage over the currently used Reichstein and Grussn ...
Biochemistry 1997 Baird
... the dehydration of serum bicarbonate to CO2 (Whitney & Briggle, 1982). Furthermore, CA IV is found in the endothelial cells of an ocular capillary bed (Hageman et al., 1991; Maren et al., 1993), suggesting that this isozyme may also be a target for carbonic anhydrase inhibitors that are used in the ...
... the dehydration of serum bicarbonate to CO2 (Whitney & Briggle, 1982). Furthermore, CA IV is found in the endothelial cells of an ocular capillary bed (Hageman et al., 1991; Maren et al., 1993), suggesting that this isozyme may also be a target for carbonic anhydrase inhibitors that are used in the ...
PDF - DigiNole! - Florida State University
... However, there is evidence that the enzyme recognizes and stabilizes the keto- instead of the gem-diol form of the substrate.13 This is also supported by the crystal structures of substrate complexes of other enzymes belonging to this family, which reveal that these enzymes recognize the keto form o ...
... However, there is evidence that the enzyme recognizes and stabilizes the keto- instead of the gem-diol form of the substrate.13 This is also supported by the crystal structures of substrate complexes of other enzymes belonging to this family, which reveal that these enzymes recognize the keto form o ...
Recombinant thrombin receptor and related pharmaceuticals
... present in the thrombin ligand can be included in antagonists Which occupy the binding region of the receptor and thus prevent binding of thrombin. A?fth group of antagonists Will include antibodies Which are designed to bind speci?c regions of receptor protein. In general, these are monoclonal anti ...
... present in the thrombin ligand can be included in antagonists Which occupy the binding region of the receptor and thus prevent binding of thrombin. A?fth group of antagonists Will include antibodies Which are designed to bind speci?c regions of receptor protein. In general, these are monoclonal anti ...
The Impact of Ligand Design on the Coordination Chemistry and
... I especially want to thank my advisor, Prof.James R. Gardinier for his guidance during my research and study at Marquette University. His perpetual energy and enthusiasm in research had motivated all his advisees, including me. Besides my advisor, I would like to thank the rest of my dissertation co ...
... I especially want to thank my advisor, Prof.James R. Gardinier for his guidance during my research and study at Marquette University. His perpetual energy and enthusiasm in research had motivated all his advisees, including me. Besides my advisor, I would like to thank the rest of my dissertation co ...
CHM 423 Coordination Chemistry
... from the nature of chemical bond that leads to the formation of these compounds. This chemical bond called coordinate covalent bond involves donation of electron pair(s) by a molecule or negatively charged ion, a Lewis base, to a neutral metal or positively charged ion, a Lewis acid. These compounds ...
... from the nature of chemical bond that leads to the formation of these compounds. This chemical bond called coordinate covalent bond involves donation of electron pair(s) by a molecule or negatively charged ion, a Lewis base, to a neutral metal or positively charged ion, a Lewis acid. These compounds ...
as a PDF
... sequence identified an additional operon (yczA-ycbK) with a presumed TRAP binding site (35). This site contains as many as 11 triplet repeats (7 GAG, 3 AAG, and 1 CAG) that overlap the ycbK SD sequence and translation initiation region. Furthermore, the features of this operon suggested that it coul ...
... sequence identified an additional operon (yczA-ycbK) with a presumed TRAP binding site (35). This site contains as many as 11 triplet repeats (7 GAG, 3 AAG, and 1 CAG) that overlap the ycbK SD sequence and translation initiation region. Furthermore, the features of this operon suggested that it coul ...
Investigating The Metal Binding Sites In Znta, A Zinc Transporting
... administrative help and making the life of an international graduate student very comfortable. To my lab mates both past and present, the support and encouragement that I received from them has been enormous. I would like to thank Aster Dewanti and Junbo Liu, for helping me during my early days in ...
... administrative help and making the life of an international graduate student very comfortable. To my lab mates both past and present, the support and encouragement that I received from them has been enormous. I would like to thank Aster Dewanti and Junbo Liu, for helping me during my early days in ...
Vanadium-Binding Proteins (Vanabins)
... The metal concentration in each fraction was determined by atomic absorption spectrophotometry (AAS, SAS–7500, Seiko Instruments Inc.), and the protein concentration was determined with BioRad reagent (Bio-Rad Laboratories Inc.). To analyze vanadium by AAS, 10 μl of each sample, diluted if necessary ...
... The metal concentration in each fraction was determined by atomic absorption spectrophotometry (AAS, SAS–7500, Seiko Instruments Inc.), and the protein concentration was determined with BioRad reagent (Bio-Rad Laboratories Inc.). To analyze vanadium by AAS, 10 μl of each sample, diluted if necessary ...
Biochemical Journal
... (HindIII restriction site underlined). The gene fragment was digested by NdeI/HindIII and inserted into the pET-28b expression plasmid resulting in N-terminal His6 (hexahistidine)-tagged pET28b-LiCMS. The plasmid was transformed into Escherichia coli BL21(DE3) strain (Novagen) and the transformed ba ...
... (HindIII restriction site underlined). The gene fragment was digested by NdeI/HindIII and inserted into the pET-28b expression plasmid resulting in N-terminal His6 (hexahistidine)-tagged pET28b-LiCMS. The plasmid was transformed into Escherichia coli BL21(DE3) strain (Novagen) and the transformed ba ...
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... cardiovascular system. It is clear that thrombin aids in the formation of blood clots by catalyzing the conversion of ?brinogen to ?brin. which is an integral part of most clots. In addition. thrombin is known to act directly on cells in the blood and in the interior blood vessel wall. and speci?cal ...
... cardiovascular system. It is clear that thrombin aids in the formation of blood clots by catalyzing the conversion of ?brinogen to ?brin. which is an integral part of most clots. In addition. thrombin is known to act directly on cells in the blood and in the interior blood vessel wall. and speci?cal ...
Full-Text PDF
... analyzed by LC-MS/MS. Two chromatographic peaks at 300-400 nm (Sal absorption) were detected (data not shown), and each of them was identified as the Sal-conjugated T7 phage-displayed peptide by tandem mass spectrometry (Figure 4). Also these data suggest that two Sal molecules were conjugated to th ...
... analyzed by LC-MS/MS. Two chromatographic peaks at 300-400 nm (Sal absorption) were detected (data not shown), and each of them was identified as the Sal-conjugated T7 phage-displayed peptide by tandem mass spectrometry (Figure 4). Also these data suggest that two Sal molecules were conjugated to th ...