SBT202 - SibTech, Inc.
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
Molecular forms of prostate specific antigen (PSA) in serum: clinical
... interchangeable, not uniformly standardised, and with no widely accepted conversion factor to correct the accuracy. Large discrepancies in fPSA values may result in clinical misinterpretation as the decision to consider a prostate biopsy may be based on the ratio of fPSA to tPSA. Persisting discrepa ...
... interchangeable, not uniformly standardised, and with no widely accepted conversion factor to correct the accuracy. Large discrepancies in fPSA values may result in clinical misinterpretation as the decision to consider a prostate biopsy may be based on the ratio of fPSA to tPSA. Persisting discrepa ...
THE ATP SYNTHASE—A SPLENDID MOLECULAR MACHINE
... and chemical derivatization studies. Crystallization was in the presence of Mg2+ , 5 µM ADP, and 250 µM AMP-PNP. One catalytic site is filled with ADP, another with AMP-PNP, and a third site is empty. All three noncatalytic sites have bound AMP-PNP. Below a central dimple of about 15 Å, the core is ...
... and chemical derivatization studies. Crystallization was in the presence of Mg2+ , 5 µM ADP, and 250 µM AMP-PNP. One catalytic site is filled with ADP, another with AMP-PNP, and a third site is empty. All three noncatalytic sites have bound AMP-PNP. Below a central dimple of about 15 Å, the core is ...
Biosensors
... an internal reflection element (IRE), causing attenuation of the internally reflected light. ATR has been used for immunoassays in the IR, visible, and UV regions. ...
... an internal reflection element (IRE), causing attenuation of the internally reflected light. ATR has been used for immunoassays in the IR, visible, and UV regions. ...
Document
... Regulation of ethylene synthesis and activity Ethylene level : Metabolism / Environment ...
... Regulation of ethylene synthesis and activity Ethylene level : Metabolism / Environment ...
Late events of translation initiation in bacteria: a kinetic analysis
... ¯uorescence stopped-¯ow kinetic techniques. IF2dependent GTP hydrolysis was fast (30/s) followed by slow Pi release from the complex (1.5/s). The latter step was rate limiting for subsequent A-site binding of EF-Tu´GTP´Phe-tRNAPhe ternary complex. Most of the elemental rate constants of A-site bindi ...
... ¯uorescence stopped-¯ow kinetic techniques. IF2dependent GTP hydrolysis was fast (30/s) followed by slow Pi release from the complex (1.5/s). The latter step was rate limiting for subsequent A-site binding of EF-Tu´GTP´Phe-tRNAPhe ternary complex. Most of the elemental rate constants of A-site bindi ...
![Comparison of Ligand-Binding Sites of Modeled Apo[a] Kringle](http://s1.studyres.com/store/data/022146975_1-ba8d6ebf00813a811b124aa3a02c9785-300x300.png)
Comparison of Ligand-Binding Sites of Modeled Apo[a] Kringle
... Lp[a] contains two major and distinctly different highmolecular-weight apoproteins.5-7 Within Lp[a], apoprotein[a], apo[a], a highly glycosylated, hydrophilic protein with little apparent affinity for lipid, is disulfide-linked to one molecule of apo B-100,68-13 a highly hydrophobic apolipoprotein. ...
... Lp[a] contains two major and distinctly different highmolecular-weight apoproteins.5-7 Within Lp[a], apoprotein[a], apo[a], a highly glycosylated, hydrophilic protein with little apparent affinity for lipid, is disulfide-linked to one molecule of apo B-100,68-13 a highly hydrophobic apolipoprotein. ...
Metal Complexes Containing Natural and Artificial Radioactive
... discussed from the view point of their synthesis, properties, and main applications. On the basis of their properties, several mono-, bi-, tri-, tetra- or polydentate ligands have been designed for specific recognition of some particular radionuclides, and can be used in the processes of nuclear was ...
... discussed from the view point of their synthesis, properties, and main applications. On the basis of their properties, several mono-, bi-, tri-, tetra- or polydentate ligands have been designed for specific recognition of some particular radionuclides, and can be used in the processes of nuclear was ...
Naphtyl-imidazo-anthraquinones as novel colorimetric
... was a fluorescence quenching. Considering recognition in organic aqueous mixture, it was found that selectivity for CN- was achieved for both receptors, with an easily detectable colour change from yellow to orange. Compounds 1-2 in their deprotonated form, after fluoride addition, were studied as m ...
... was a fluorescence quenching. Considering recognition in organic aqueous mixture, it was found that selectivity for CN- was achieved for both receptors, with an easily detectable colour change from yellow to orange. Compounds 1-2 in their deprotonated form, after fluoride addition, were studied as m ...
- Murdoch Research Repository
... recognition or intracellular processing allowing the virus to escape detection by the host immune system (see figure 4 & 5). ...
... recognition or intracellular processing allowing the virus to escape detection by the host immune system (see figure 4 & 5). ...
coordination of some monodentate and hybrid multident ate
... been prepared and characterised by a combination of *H, [P and 19F (as appropriate) NMR and IR spectroscopies and mass spectrometry. Some of these complexes have been isolated as single crystals and characterised by X-ray diffraction. The triarylphosphine ligands used in this study were P(4 -CH3 0 C ...
... been prepared and characterised by a combination of *H, [P and 19F (as appropriate) NMR and IR spectroscopies and mass spectrometry. Some of these complexes have been isolated as single crystals and characterised by X-ray diffraction. The triarylphosphine ligands used in this study were P(4 -CH3 0 C ...
Microsoft Word
... regions located on the TPO molecule surface [5, 8]. Antibodies numbered 2, 9, 47, and 60 reacted with epitopes in domain A, mAbs 15, 18, 59, and 64 recognized domain B, mAb 24 bound to IDR-C, and 1, 30, 40, and 53 to IDR-D [5]. When rabbit antisera raised to the linear peptides exposed on the TPO su ...
... regions located on the TPO molecule surface [5, 8]. Antibodies numbered 2, 9, 47, and 60 reacted with epitopes in domain A, mAbs 15, 18, 59, and 64 recognized domain B, mAb 24 bound to IDR-C, and 1, 30, 40, and 53 to IDR-D [5]. When rabbit antisera raised to the linear peptides exposed on the TPO su ...
Scalable graphene field-effect sensors for specific protein detection
... microfluidic delivery system and subjected to the same set of measurement protocols. As shown in the bottom panel of figure 4, the bio-recognition PBASE–aptamer element on graphene is reusable, retaining the same biosensing affinity with KD ∼ 200 nM. In conclusion, large-area graphene derived from t ...
... microfluidic delivery system and subjected to the same set of measurement protocols. As shown in the bottom panel of figure 4, the bio-recognition PBASE–aptamer element on graphene is reusable, retaining the same biosensing affinity with KD ∼ 200 nM. In conclusion, large-area graphene derived from t ...
Properties and Kinetic Analysis of UDP
... 8.7, containing 2 mM dithiothreitol). To obtain a clean spectrum of the enzyme it was necessary to prevent the aerobic oxidation of dithiothreitol by carrying out the dialysis under an atmosphere of argon. The buffer was degassed and thoroughly sparged with argon prior to the addition of solid dithi ...
... 8.7, containing 2 mM dithiothreitol). To obtain a clean spectrum of the enzyme it was necessary to prevent the aerobic oxidation of dithiothreitol by carrying out the dialysis under an atmosphere of argon. The buffer was degassed and thoroughly sparged with argon prior to the addition of solid dithi ...
Serine racemase: a KEY PLAYER in NEURON activity and in
... solution SR forms dimers with traces of tetramers (12). Dimers are stabilized by the formation of hydrophobic contacts, without the involvement of any disulfide bridge (20-22). Covalently cross-linked dimers were observed in the presence of reactive oxygen and nitrogen species (23). The human and ra ...
... solution SR forms dimers with traces of tetramers (12). Dimers are stabilized by the formation of hydrophobic contacts, without the involvement of any disulfide bridge (20-22). Covalently cross-linked dimers were observed in the presence of reactive oxygen and nitrogen species (23). The human and ra ...
The acetaminophen metabolite
... GS co-incubated with NAPQI was also investigated. After pre-incubating water or NAPQI (200 μM) with GS for 30 min at 37 ºC, 10 uL was transferred to the standard assay mixture, but with addition of normal or twice the normal amounts of PK or LDH. GS activity was measured by the standard procedure. P ...
... GS co-incubated with NAPQI was also investigated. After pre-incubating water or NAPQI (200 μM) with GS for 30 min at 37 ºC, 10 uL was transferred to the standard assay mixture, but with addition of normal or twice the normal amounts of PK or LDH. GS activity was measured by the standard procedure. P ...
Molecular insights into RNA and DNA helicase evolution from the
... eLife digest Living cells store their genetic material as DNA, which can be copied to make another molecule called RNA. DNA consists of two strands that are wound around each other in a double helix. RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a ...
... eLife digest Living cells store their genetic material as DNA, which can be copied to make another molecule called RNA. DNA consists of two strands that are wound around each other in a double helix. RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a ...
The Department of Electrical and Computer Engineering
... Figure 2.2 Illustration of protein occupying the surface of a carbon nanotube in helical manner (Balavoine et al.,1999 [50]). ......................................................................................................... 13 Figure 2.3 Demonstration of the conjugation process of proteins t ...
... Figure 2.2 Illustration of protein occupying the surface of a carbon nanotube in helical manner (Balavoine et al.,1999 [50]). ......................................................................................................... 13 Figure 2.3 Demonstration of the conjugation process of proteins t ...
Synthesis, Structure and functions of hemoglobin Learning
... Hemoglobin A (α2β2) The most common with a normal amount over 95% Hemoglobin A2 (α2δ2) - δ chain synthesis begins late in the third trimester and in adults, it has a normal range of 1.5-3.5% Hemoglobin F (α2γ2) - In adults Hemoglobin F is restricted to a limited population of red cells called Fcells ...
... Hemoglobin A (α2β2) The most common with a normal amount over 95% Hemoglobin A2 (α2δ2) - δ chain synthesis begins late in the third trimester and in adults, it has a normal range of 1.5-3.5% Hemoglobin F (α2γ2) - In adults Hemoglobin F is restricted to a limited population of red cells called Fcells ...
Structure of the Actin-Myosin Complex and Its Implications for
... ments have demonstrated that transduction of the energy released by ATP hydrolysis into directed mechanical force occurs during product release-adenosine diphosphate (ADP) and inorganic phosphate, P,-rather than during the hydrolysis step itself (3, 4). The contractile cycle deduced from kinetic stu ...
... ments have demonstrated that transduction of the energy released by ATP hydrolysis into directed mechanical force occurs during product release-adenosine diphosphate (ADP) and inorganic phosphate, P,-rather than during the hydrolysis step itself (3, 4). The contractile cycle deduced from kinetic stu ...
thyroid synthesis, mode of action, metabolic functions and disorders
... • Each thyroglobulin molecule contains up to 30 thyroxine molecules and a few triiodothyronine molecules. • Stored Thyroid Hormones maintain the body’s requirement of T3 and T4 for up to 2-3 months Release of Thyroid Hormones • Thyroglobulin itself is not secreted into the circulation • Thyroglobuli ...
... • Each thyroglobulin molecule contains up to 30 thyroxine molecules and a few triiodothyronine molecules. • Stored Thyroid Hormones maintain the body’s requirement of T3 and T4 for up to 2-3 months Release of Thyroid Hormones • Thyroglobulin itself is not secreted into the circulation • Thyroglobuli ...
Carbamoyl Phosphate Synthetase: Closure of the B
... 933, respectively) are topologically similar but not identical to one another as can be seen in Figure 2a. This observation is in full accord with the different biochemical functions required of these two synthetase components. Each one of these two active sites must utilize Mg2+ATP to phosphorylate ...
... 933, respectively) are topologically similar but not identical to one another as can be seen in Figure 2a. This observation is in full accord with the different biochemical functions required of these two synthetase components. Each one of these two active sites must utilize Mg2+ATP to phosphorylate ...
Incomplete citric acid cycle obliges aminolevulinic
... to oxaloacetate is incomplete, lacking 2-oxoglutarate and succinate and malate dehydrogenase activities. Furthermore, alternative routes of 2-oxoglutarate oxidation to succinate are undetectable. The enzymes of the glyoxylate cycle are also absent. This suggests that the cycle in M. methylotrophus h ...
... to oxaloacetate is incomplete, lacking 2-oxoglutarate and succinate and malate dehydrogenase activities. Furthermore, alternative routes of 2-oxoglutarate oxidation to succinate are undetectable. The enzymes of the glyoxylate cycle are also absent. This suggests that the cycle in M. methylotrophus h ...