Fluorescence Study of Bovine β-Lactoglobulin
... near the pocket [1-5]. In order to find out whether both Trp residues contribute to β-lactoglobulin fluorescence or not, time-resolved studies and static quenching were performed in presence of high concentrations of calcofluor white, a fluorophore that is specific to both carbohydrate residues and ...
... near the pocket [1-5]. In order to find out whether both Trp residues contribute to β-lactoglobulin fluorescence or not, time-resolved studies and static quenching were performed in presence of high concentrations of calcofluor white, a fluorophore that is specific to both carbohydrate residues and ...
Probing Allosteric Binding Sites of the Maize
... Maize (Zea mays) endosperm ADP-glucose pyrophosphorylase (AGPase) is a highly regulated enzyme that catalyzes the ratelimiting step in starch biosynthesis. Although the structure of the heterotetrameric maize endosperm AGPase remains unsolved, structures of a nonnative, low-activity form of the pota ...
... Maize (Zea mays) endosperm ADP-glucose pyrophosphorylase (AGPase) is a highly regulated enzyme that catalyzes the ratelimiting step in starch biosynthesis. Although the structure of the heterotetrameric maize endosperm AGPase remains unsolved, structures of a nonnative, low-activity form of the pota ...
From Structure to Function
... the protein with other molecules in the signaling or transport pathway. To the geneticist or cell biologist, function includes these roles but will also encompass the cellular roles of the protein, as judged by the phenotype of its deletion, for example, or the pathway in which it operates. A physio ...
... the protein with other molecules in the signaling or transport pathway. To the geneticist or cell biologist, function includes these roles but will also encompass the cellular roles of the protein, as judged by the phenotype of its deletion, for example, or the pathway in which it operates. A physio ...
Cyclam ``capa` POT.4` to ``capa` POT.3` denticity change
... decided, therefore, to study Ru complexes with mono-Nsubstituted cyclam ligands containing an arm bearing amine or carboxy functional groups. These groups are versatile linkers because they can form amide bonds with a desired material or relevant biomolecules such as proteins or antibodies. Whereas ...
... decided, therefore, to study Ru complexes with mono-Nsubstituted cyclam ligands containing an arm bearing amine or carboxy functional groups. These groups are versatile linkers because they can form amide bonds with a desired material or relevant biomolecules such as proteins or antibodies. Whereas ...
The initiation phase of protein synthesis in eukaryotes
... recombinant murine eIF-4E in buffer A. The resin was washed in buffer A (3XHaghighat et al., then 1995 incub for 60 min at 4° C. The resin was washed in buffer A (3x 1 ml) and incubated further with buffer A ...
... recombinant murine eIF-4E in buffer A. The resin was washed in buffer A (3XHaghighat et al., then 1995 incub for 60 min at 4° C. The resin was washed in buffer A (3x 1 ml) and incubated further with buffer A ...
Monday 10-1 Lecture 1 Q: Thanks for podcasting How can one not
... Both can happen. Lock and key implies that the binding site exists independent of the ligand being present, and this is often the case. Induced fit (as we will talk about in class) happens when the ligand causes the structure of the enzyme to change to make a better binding site for the substrate or ...
... Both can happen. Lock and key implies that the binding site exists independent of the ligand being present, and this is often the case. Induced fit (as we will talk about in class) happens when the ligand causes the structure of the enzyme to change to make a better binding site for the substrate or ...
Fluorescent Probe Studies of Proteins
... On the day of the phosphorylase kinase assays, dilute phosphorylase b into the stock buffer for a protein concentration of 5.2 mg/ml. Dithiothreitol (0.2 M stock) is then added to a final concentration of 2 mM. Important: Add a small amount of charcoal (~0.5 mg/ml; Norit A) to the solution in order ...
... On the day of the phosphorylase kinase assays, dilute phosphorylase b into the stock buffer for a protein concentration of 5.2 mg/ml. Dithiothreitol (0.2 M stock) is then added to a final concentration of 2 mM. Important: Add a small amount of charcoal (~0.5 mg/ml; Norit A) to the solution in order ...
Microenvironment analysis and identification of magnesium binding
... possible molecular folds that exist in nature. Although, the number of known RNA structural motifs is less than those for proteins, structures of RNA±protein complexes, such as the ribosome, are providing numerous examples of these motifs (29±34), and causing increased interest in computational meth ...
... possible molecular folds that exist in nature. Although, the number of known RNA structural motifs is less than those for proteins, structures of RNA±protein complexes, such as the ribosome, are providing numerous examples of these motifs (29±34), and causing increased interest in computational meth ...
The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA
... The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA assays as measured on the Qubit Fluorometer Glycogen (a branched-chain carbohydrate; Cat. No. 10814010) and Invitrogen™ GlycoBlue™ Coprecipitant (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to fa ...
... The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA assays as measured on the Qubit Fluorometer Glycogen (a branched-chain carbohydrate; Cat. No. 10814010) and Invitrogen™ GlycoBlue™ Coprecipitant (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to fa ...
Implications For Transition-State Analogs And Catalytic
... Ulrich, H.D., Mundorff, E.C., Santarsiero, B.D., Driggers, E.M., Stevens, R.C., and ...
... Ulrich, H.D., Mundorff, E.C., Santarsiero, B.D., Driggers, E.M., Stevens, R.C., and ...
CBS2005_AMJ - Center for Biological Sequence Analysis
... … is a compound (ligand) which binds to a protein, often a receptor and in this way either initiates a process (agonists) or inhibits the natural signal transmitters in binding (antagonists) The structure/conformation of the ligand is complementary to the space defined by the proteins active site ...
... … is a compound (ligand) which binds to a protein, often a receptor and in this way either initiates a process (agonists) or inhibits the natural signal transmitters in binding (antagonists) The structure/conformation of the ligand is complementary to the space defined by the proteins active site ...
Journal of Molecular Biology
... for bond lengths and angles of 0.012 Å and 1.6598, respectively. The overall mean B value is 15.9 Å2. The asymmetric unit contains four crystallographically independent protein molecules, each showing clear electron density of four b-D-glucosyl residues in the substrate binding cleft, covering sub ...
... for bond lengths and angles of 0.012 Å and 1.6598, respectively. The overall mean B value is 15.9 Å2. The asymmetric unit contains four crystallographically independent protein molecules, each showing clear electron density of four b-D-glucosyl residues in the substrate binding cleft, covering sub ...
Using antibody catalysis to study the outcome of multiple
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
Molecular docking study of naturally
... On the other hand, NMT–flavonoids interaction values were found to be highly variable (ranging between –8.4 to –10.7 kcal/mol; RSD = 8.4%) demonstrating that affinity depends on structure. On comparing 16, 17 and 18, structure–affinity relationships can be observed; 17 and 18 affinities exhibited id ...
... On the other hand, NMT–flavonoids interaction values were found to be highly variable (ranging between –8.4 to –10.7 kcal/mol; RSD = 8.4%) demonstrating that affinity depends on structure. On comparing 16, 17 and 18, structure–affinity relationships can be observed; 17 and 18 affinities exhibited id ...
BEL β-trefoil: A novel lectin with antineoplastic properties in king
... called lectins (Sharon 2007). In general, ligand binding precedes the fulfillment of an important biological function, which in some cases is still not known. Some members of this family are also called agglutinins because of their ability to agglutinate red blood cells, but this term does not necess ...
... called lectins (Sharon 2007). In general, ligand binding precedes the fulfillment of an important biological function, which in some cases is still not known. Some members of this family are also called agglutinins because of their ability to agglutinate red blood cells, but this term does not necess ...
Recombinant N-terminal Nucleotide
... parasite (9) drug transporters, and a series of different members from eukaryotic proteins, like the cystic fibrosis gene product CFTR, to bacterial transporters (10). The ATPase activity and related drug transport of P-glycoprotein require both functional nucleotide-binding sites (11, 12) and are s ...
... parasite (9) drug transporters, and a series of different members from eukaryotic proteins, like the cystic fibrosis gene product CFTR, to bacterial transporters (10). The ATPase activity and related drug transport of P-glycoprotein require both functional nucleotide-binding sites (11, 12) and are s ...
Single-Amino Acid Substitutions Alter the Specificity and Affinity of
... 1 Abbreviations: PDZ, PSD-95/Dlg/ZO-1; PSD-95, 95 kDa postsynaptic density protein; nNOS, neuronal nitric oxide synthase; Dlg, discslarge; ZO-1, Zona Occludens-1; PH, pleckstrin homology; SU, syntrophin unique; GST, glutathione S-transferase; PCR, polymerase chain reaction; SPR, surface plasmon reso ...
... 1 Abbreviations: PDZ, PSD-95/Dlg/ZO-1; PSD-95, 95 kDa postsynaptic density protein; nNOS, neuronal nitric oxide synthase; Dlg, discslarge; ZO-1, Zona Occludens-1; PH, pleckstrin homology; SU, syntrophin unique; GST, glutathione S-transferase; PCR, polymerase chain reaction; SPR, surface plasmon reso ...
Role of Na and K in Enzyme Function
... pairs in the valence shell (Table 2). They have low electron affinity and a strong tendency for hydration. Both the strong charge and small ionic radius of group IA metals impart bonding characteristics that are more covalent in nature. However, interaction between M⫹ and ligand is based solely on e ...
... pairs in the valence shell (Table 2). They have low electron affinity and a strong tendency for hydration. Both the strong charge and small ionic radius of group IA metals impart bonding characteristics that are more covalent in nature. However, interaction between M⫹ and ligand is based solely on e ...
Lipoproteins
... Lipoprotein Lipase, is attached to the luminal surface of small blood vessels. It catalyzes hydrolytic cleavage of fatty acids from triacylglycerols of chylomicrons. Released fatty acids & monoacylglycerols are picked up by body cells for use as energy sources. With removal of triacylglycerols and ...
... Lipoprotein Lipase, is attached to the luminal surface of small blood vessels. It catalyzes hydrolytic cleavage of fatty acids from triacylglycerols of chylomicrons. Released fatty acids & monoacylglycerols are picked up by body cells for use as energy sources. With removal of triacylglycerols and ...
Microsoft Word
... [45,46], combination of the crystallographic structure with such G analysis provided molecular mechanism of specific binding between the cap and eIF4E [12]. However, applying the procedure to detect the discrimination mechanism between MMG- and TMG-cap by some eIF4Es [17,18] has failed. The struct ...
... [45,46], combination of the crystallographic structure with such G analysis provided molecular mechanism of specific binding between the cap and eIF4E [12]. However, applying the procedure to detect the discrimination mechanism between MMG- and TMG-cap by some eIF4Es [17,18] has failed. The struct ...
This is the accepted version of the following article:
... modification of isolated protein. Purifications based on affinity chromatography represent the most powerful tool available in term of the selectivity and recovery. Batch separation with specific antibodies as affinity ligand immobilized on magnetic carriers (i. e. immunomagnetic separation, IMS) ha ...
... modification of isolated protein. Purifications based on affinity chromatography represent the most powerful tool available in term of the selectivity and recovery. Batch separation with specific antibodies as affinity ligand immobilized on magnetic carriers (i. e. immunomagnetic separation, IMS) ha ...
High-resolution mapping of protein sequence
... encodes 25 variable residues that span the structured region of the WW domain, including both of the conserved tryptophan residues, and encompass the binding interface (Fig. 1a). We acquired sequencing data for the input variant library as well as after three and six rounds of selection, with an ave ...
... encodes 25 variable residues that span the structured region of the WW domain, including both of the conserved tryptophan residues, and encompass the binding interface (Fig. 1a). We acquired sequencing data for the input variant library as well as after three and six rounds of selection, with an ave ...
Ser Trp Thr His Glu Asn Gly Lys His Val Trp Arg
... Biomolecular folding is a type of molecular self-assembly in which self-interaction drives a molecule to its native conformation. We synthesized six 12-amino acid β-hairpin peptides stabilized by π-π interactions between the indole groups of two tryptophan residues. Histidine binding pockets of vary ...
... Biomolecular folding is a type of molecular self-assembly in which self-interaction drives a molecule to its native conformation. We synthesized six 12-amino acid β-hairpin peptides stabilized by π-π interactions between the indole groups of two tryptophan residues. Histidine binding pockets of vary ...
Allosteric pathways in imidazole glycerol phosphate synthase
... and/or thermodynamic parameters of the biochemical reaction in the active site. Allosteric information transfer can range from large, enthalpically driven conformational changes to purely entropically driven motions or a combination of both enthalpic and entropic effects, but in each case the kineti ...
... and/or thermodynamic parameters of the biochemical reaction in the active site. Allosteric information transfer can range from large, enthalpically driven conformational changes to purely entropically driven motions or a combination of both enthalpic and entropic effects, but in each case the kineti ...
A Survey of Flexible Protein Binding Mechanisms and their
... size of the protein needs to be more than twice as large as one with a small interface, resulting in increased cellular crowding.38 Accordingly, disordered proteins provide a simple solution for having large inter-molecular interfaces while maintaining a small genome. A kinetic advantage for being i ...
... size of the protein needs to be more than twice as large as one with a small interface, resulting in increased cellular crowding.38 Accordingly, disordered proteins provide a simple solution for having large inter-molecular interfaces while maintaining a small genome. A kinetic advantage for being i ...