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... Example of Ping-Pong Enzyme: Aspartate aminotransferase: Catalyzes the conversion of aspartate to glutamate with production of oxaloacetate and consumption of alphaketoglutarate. The reaction sequence starts with the binding of aspartate to the enzyme followed by its conversion to oxaloacetate, in t ...
... Example of Ping-Pong Enzyme: Aspartate aminotransferase: Catalyzes the conversion of aspartate to glutamate with production of oxaloacetate and consumption of alphaketoglutarate. The reaction sequence starts with the binding of aspartate to the enzyme followed by its conversion to oxaloacetate, in t ...
Substrate specificity of the periplasmic dipeptide
... Pure dipeptide-binding protein (DppA) from Escherichia coli was studied in a filter binding assay to determine its binding specificity. A substrate :DppA stoichiometry of 1 :1 was found with both [14C]AlaAla and Ala[14C]Phe. Surprisingly, substrate binding did not vary over the pH range pH 3–95. Di ...
... Pure dipeptide-binding protein (DppA) from Escherichia coli was studied in a filter binding assay to determine its binding specificity. A substrate :DppA stoichiometry of 1 :1 was found with both [14C]AlaAla and Ala[14C]Phe. Surprisingly, substrate binding did not vary over the pH range pH 3–95. Di ...
1 Development of Bombesin Analogues With Conformationally
... Ryan et al., 1998a). Briefly, hBRS-3, hGRP-R or hNMB-R-transfected BALB 3T3 cells were subcultured into 24-well plates (5 x 104 cells/well) in regular propagation media and then incubated for 24 hr at 37oC in a 5% CO2 atmosphere. The cells were then incubated with 3 µCi/ml of myo-[2-2H] inositol in ...
... Ryan et al., 1998a). Briefly, hBRS-3, hGRP-R or hNMB-R-transfected BALB 3T3 cells were subcultured into 24-well plates (5 x 104 cells/well) in regular propagation media and then incubated for 24 hr at 37oC in a 5% CO2 atmosphere. The cells were then incubated with 3 µCi/ml of myo-[2-2H] inositol in ...
Biochemists Break the Code
... Binding of a new aa-tRNA at the A site -EF-Tu (GTP), EF-Ts Formation of the new peptide bond (Transpeptidation)-23S rRNA Translocation of the Ribosome -EF-G(GTP) Repeat and Repeat until the stop codon enters the A site Binding of a new aa-tRNA at the A site At the start of each cycle, the A site on ...
... Binding of a new aa-tRNA at the A site -EF-Tu (GTP), EF-Ts Formation of the new peptide bond (Transpeptidation)-23S rRNA Translocation of the Ribosome -EF-G(GTP) Repeat and Repeat until the stop codon enters the A site Binding of a new aa-tRNA at the A site At the start of each cycle, the A site on ...
Glucose-6-Phosphate Fluorometric Assay Kit
... This material should be considered hazardous until further information becomes available. Do not ingest, inhale, get in eyes, on skin, or on clothing. Wash thoroughly after handling. Before use, the user must review the complete Safety Data Sheet, which has been sent via email to your institution. ...
... This material should be considered hazardous until further information becomes available. Do not ingest, inhale, get in eyes, on skin, or on clothing. Wash thoroughly after handling. Before use, the user must review the complete Safety Data Sheet, which has been sent via email to your institution. ...
Mechanisms of Unidirectional Translocation & Unwinding
... tight binding of helicase to NA. The other is Brownian motion that can move the helicase in any direction along the NA while the helicase binding is in a flat energy profile. • Brownian model predicts limited processivity for monomeric helicases. • Dimeric or hexameric helicases with 2 or 6 ssNA bin ...
... tight binding of helicase to NA. The other is Brownian motion that can move the helicase in any direction along the NA while the helicase binding is in a flat energy profile. • Brownian model predicts limited processivity for monomeric helicases. • Dimeric or hexameric helicases with 2 or 6 ssNA bin ...
Ionized and neutral donor bound excitons: Haynes´ rule
... ago1-4, 5. More than 12 individual excitonic transitions have been reported. For only four of them an unambiguous assignment as to the chemical nature of the defects involved could be given6. It is, therefore, of prime interest to gain further insight into the excitonic recombinations especially wit ...
... ago1-4, 5. More than 12 individual excitonic transitions have been reported. For only four of them an unambiguous assignment as to the chemical nature of the defects involved could be given6. It is, therefore, of prime interest to gain further insight into the excitonic recombinations especially wit ...
Complex forming reactions and complexometry Complex forming
... The following conditions need to be met for the successful use of a complex reaction in titrimetry: • the complex needs to be very stable, with a high formation constant so the reaction is stochiometric (chelates) • the solution needs to be buffered • the complex needs to be formed in 1:1 ratio (for ...
... The following conditions need to be met for the successful use of a complex reaction in titrimetry: • the complex needs to be very stable, with a high formation constant so the reaction is stochiometric (chelates) • the solution needs to be buffered • the complex needs to be formed in 1:1 ratio (for ...
Chapter 6: Metal induced selectivity in phosphate ion binding in
... released by Escherichia coli into the extracellular medium during times of nutrient or environmental stress. On entrance into target cells the 15-kDa C-terminal DNase domain is translocated into the cytoplasm. In the target cell the lethal effect is accomplished by the hydrolysis of chromosomal DNA. ...
... released by Escherichia coli into the extracellular medium during times of nutrient or environmental stress. On entrance into target cells the 15-kDa C-terminal DNase domain is translocated into the cytoplasm. In the target cell the lethal effect is accomplished by the hydrolysis of chromosomal DNA. ...
Localization of the prostaglandin F2 alpha receptor
... receptor was raised in rabbits and the immunoglobulin (Ig) G fraction was used. Specificity of the antibody was determined using stable FP receptor-transfected versus nontransfected host cells and by competition with its corresponding antigen. Specificity also was seen on Western blots of yeast-expr ...
... receptor was raised in rabbits and the immunoglobulin (Ig) G fraction was used. Specificity of the antibody was determined using stable FP receptor-transfected versus nontransfected host cells and by competition with its corresponding antigen. Specificity also was seen on Western blots of yeast-expr ...
Modulating the cobalt redox potential through imidazole hydrogen
... and 1-H+ (375 vs. 150 Hz) is due to the distinct nuclear quadrupolar properties of 59Co, which signicantly increase the t1 and t2 relaxation rates of the directly bound carbon. These effects also prevent the observation of resolved 13C-multiplets due to scalar coupling between 13C and 59Co. Other br ...
... and 1-H+ (375 vs. 150 Hz) is due to the distinct nuclear quadrupolar properties of 59Co, which signicantly increase the t1 and t2 relaxation rates of the directly bound carbon. These effects also prevent the observation of resolved 13C-multiplets due to scalar coupling between 13C and 59Co. Other br ...
Study of the arginine repressor in different organisms
... The arginine repressor (ArgR) regulates transcription of the arginine biosynthetic genes in bacteria. ArgR proteins play a multifunctional role in the bacterial cell. They inhibit biosynthetic promoters and are involved in activation of several catabolic pathways. The arginine repressor of Streptomy ...
... The arginine repressor (ArgR) regulates transcription of the arginine biosynthetic genes in bacteria. ArgR proteins play a multifunctional role in the bacterial cell. They inhibit biosynthetic promoters and are involved in activation of several catabolic pathways. The arginine repressor of Streptomy ...
Membrane Penetration of Cytosolic Phospholipase A2 Is Necessary
... crucial role in maintaining cellular arachidonic acid levels (14). cPLA2 is therefore an attractive target for developing specific inhibitors that can be used as a novel antiinflammatory drugs. cPLA2 binds to membranes in the presence of micromolar Ca2+ via its C2 domain which contains calcium and m ...
... crucial role in maintaining cellular arachidonic acid levels (14). cPLA2 is therefore an attractive target for developing specific inhibitors that can be used as a novel antiinflammatory drugs. cPLA2 binds to membranes in the presence of micromolar Ca2+ via its C2 domain which contains calcium and m ...
Emmission Spectroscopy
... Basically, FRET is a great method to determine the distance between two fluorophores (molecules) in the range of ~1-10 nm. Efficiency of transfer: ...
... Basically, FRET is a great method to determine the distance between two fluorophores (molecules) in the range of ~1-10 nm. Efficiency of transfer: ...
Steroid Chemistry and Steroid Hormone Action - Rose
... It should be noted that there is evidence for cell surface receptors for some of the steroid hormones, and for some steroids that had not previously been considered to be hormones. In some experiments, responses to hormonal administration have been observed on short time scales (i.e. a few seconds t ...
... It should be noted that there is evidence for cell surface receptors for some of the steroid hormones, and for some steroids that had not previously been considered to be hormones. In some experiments, responses to hormonal administration have been observed on short time scales (i.e. a few seconds t ...
lect21
... bind to the enzyme separately, the reaction is randomorder ternary type -in most cases the rate of the first reaction is 10 – 100 times the rate of the second reactions, but in some enzymes the rates are nearly equal ...
... bind to the enzyme separately, the reaction is randomorder ternary type -in most cases the rate of the first reaction is 10 – 100 times the rate of the second reactions, but in some enzymes the rates are nearly equal ...
M 2+ ∙EDTA Binding Affinities
... Schematic diagrams for the ITC apparatus can be found online [e.g., 24-27]. Briefly, a reference cell and a sample cell are submerged in an adiabatic chamber as shown in Figure 1. One reagent is contained in the sample cell, and the other reagent is loaded into a syringe injector. Both reagents are ...
... Schematic diagrams for the ITC apparatus can be found online [e.g., 24-27]. Briefly, a reference cell and a sample cell are submerged in an adiabatic chamber as shown in Figure 1. One reagent is contained in the sample cell, and the other reagent is loaded into a syringe injector. Both reagents are ...
Sample Integrity in qPCR: Using PIPETMAX® qPCR
... it a technique that is extremely sensitive to cross-contamination and carryover. For this reason, sample integrity is crucial. A sample that has been contaminated may produce a false positive, and thereby negatively impacting critical research or a clinical analysis. The inclusion of proper controls ...
... it a technique that is extremely sensitive to cross-contamination and carryover. For this reason, sample integrity is crucial. A sample that has been contaminated may produce a false positive, and thereby negatively impacting critical research or a clinical analysis. The inclusion of proper controls ...
File
... Reaction• Condensation of acetyl-CoA and oxaloacetate • The only reaction with C-C bond formation • Rate-limiting step of CAC Mechanism• Uses Acid/Base Catalysis – Carbonyl of oxaloacetate is a good electrophile – Methyl of acetyl-CoA is NOT a good nucleophile but is activated by deprotonation ...
... Reaction• Condensation of acetyl-CoA and oxaloacetate • The only reaction with C-C bond formation • Rate-limiting step of CAC Mechanism• Uses Acid/Base Catalysis – Carbonyl of oxaloacetate is a good electrophile – Methyl of acetyl-CoA is NOT a good nucleophile but is activated by deprotonation ...
c Syun-Ru Yeh‡ and Denis L. Rousseau
... the flow cell was controlled by a micrometer on a translational stage, such that an accurate time domain could be calculated from the solution flow rate. The Raman measurements were made with previously described instrumentation (21). The output at 407 nm from a krypton ion laser (Spectra Physics) w ...
... the flow cell was controlled by a micrometer on a translational stage, such that an accurate time domain could be calculated from the solution flow rate. The Raman measurements were made with previously described instrumentation (21). The output at 407 nm from a krypton ion laser (Spectra Physics) w ...
Defining the complementarities between antibodies and haptens to
... Background: Low molecular weight haptens (<1000 Da) cannot be recognized by the immune system unless conjugated to larger carrier molecules. Antibodies to these exceptionally small antigens can still be generated with exquisite sensitivity. A detailed understanding at the molecular level of this inc ...
... Background: Low molecular weight haptens (<1000 Da) cannot be recognized by the immune system unless conjugated to larger carrier molecules. Antibodies to these exceptionally small antigens can still be generated with exquisite sensitivity. A detailed understanding at the molecular level of this inc ...
patrick_ch19_p3
... • Find suitable nucleophile capable of cleaving phosphate esters • Water is too weak as a nucleophile • Hydoxylamine is a stronger nucleophile O ...
... • Find suitable nucleophile capable of cleaving phosphate esters • Water is too weak as a nucleophile • Hydoxylamine is a stronger nucleophile O ...
Vorlesung Supramolekulare Chemie
... of the energetic balance of the binding event and most of the binding enthalpy must be used to compensate the entropic energy loss. Receptor molecule or guest binding site for intermolecular molecular recognition must be preorganized. The conformation and orientation of functional groups in the non- ...
... of the energetic balance of the binding event and most of the binding enthalpy must be used to compensate the entropic energy loss. Receptor molecule or guest binding site for intermolecular molecular recognition must be preorganized. The conformation and orientation of functional groups in the non- ...
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin
... As shown in Figure 1, both CTR2 and OPR contain the seven hydrophobic TM regions and several potential sites for N-linked glycosylation and phosphorylation that are the most typical characteristic of GPCRs. Consensus sequences for N-linked glycosylation sites (Asn-Xaa-Ser/Thr; two sites in CTR2, thr ...
... As shown in Figure 1, both CTR2 and OPR contain the seven hydrophobic TM regions and several potential sites for N-linked glycosylation and phosphorylation that are the most typical characteristic of GPCRs. Consensus sequences for N-linked glycosylation sites (Asn-Xaa-Ser/Thr; two sites in CTR2, thr ...