1 Information theory as a model of genomic sequences Chengpeng
... Shannon and Weaver (1949) developed their theory of information in order to understand the transmission of electronic signals and model the communication system. Gatlin (1972) first described its extension to biology. Information theory is an obvious tool to use in looking for patterns in DNA and pr ...
... Shannon and Weaver (1949) developed their theory of information in order to understand the transmission of electronic signals and model the communication system. Gatlin (1972) first described its extension to biology. Information theory is an obvious tool to use in looking for patterns in DNA and pr ...
1 Biochemistry 462a – Enzyme Mechanisms Reading
... o Metal-activated enzymes contain loosely bound metal ions: Na , K , Mg , Ca . Electrostatic catalysis refers to the fact that when a substrate binds to an enzyme, water is usually excluded from the active site. o This causes the local dielectric constant to be lower, o Which enhances charge-charge ...
... o Metal-activated enzymes contain loosely bound metal ions: Na , K , Mg , Ca . Electrostatic catalysis refers to the fact that when a substrate binds to an enzyme, water is usually excluded from the active site. o This causes the local dielectric constant to be lower, o Which enhances charge-charge ...
Chymotrypsin
... o Metalloenzymes contain tightly bound metal ions: Fe+2, Fe+3, Cu+2, Zn+2, Mn+2. o Metal-activated enzymes contain loosely bound metal ions: Na+, K+, Mg+2, Ca+2. Electrostatic catalysis refers to the fact that when a substrate binds to an enzyme, water is usually excluded from the active site. o Thi ...
... o Metalloenzymes contain tightly bound metal ions: Fe+2, Fe+3, Cu+2, Zn+2, Mn+2. o Metal-activated enzymes contain loosely bound metal ions: Na+, K+, Mg+2, Ca+2. Electrostatic catalysis refers to the fact that when a substrate binds to an enzyme, water is usually excluded from the active site. o Thi ...
2009/05/21 Lecture
... non-random interaction of TMD:FP association. The intra-trimeric interaction is detected for x values near 1 since nearly all peptide molecules are labeled and, therefore, quenching arises predominantly from the close neighbors within the same trimer. In contrast, for low x values, the probability o ...
... non-random interaction of TMD:FP association. The intra-trimeric interaction is detected for x values near 1 since nearly all peptide molecules are labeled and, therefore, quenching arises predominantly from the close neighbors within the same trimer. In contrast, for low x values, the probability o ...
Peroxisome Proliferator-Activated Receptors (PPARs)
... 15-deoxy-Δ12,14-Prostaglandin J2 (probably too low endogenous conc. to affect PPAR function) ? Whether a highly specific natural ligand exists or acts as a physiological lipid sensor activated by combination of weakly activating fatty acids ...
... 15-deoxy-Δ12,14-Prostaglandin J2 (probably too low endogenous conc. to affect PPAR function) ? Whether a highly specific natural ligand exists or acts as a physiological lipid sensor activated by combination of weakly activating fatty acids ...
L- Amino Acid Assay Kit (Colorimetric)
... and Val) are considered to be essential amino acids because the human body cannot make them from other molecules in enough amounts needed for normal growth, so they must be obtained from food sources. Cell Biolabs’ L-Amino Acid Assay Kit is a simple colorimetric assay that measures the total amount ...
... and Val) are considered to be essential amino acids because the human body cannot make them from other molecules in enough amounts needed for normal growth, so they must be obtained from food sources. Cell Biolabs’ L-Amino Acid Assay Kit is a simple colorimetric assay that measures the total amount ...
Specialized Functions of Major Histocompatibility Complex Class I
... Competition Assay. Target cells of the WEHI-105.7 NZB thymoma (H-2 d, Mtab) were labeled with SlCr, washed four times, and incubated for 75 rain at 37~ with increasing concentrations of the indicated peptides in the presence of 50 nM fNDll-12. Target cells were washed twice before incubation with Mt ...
... Competition Assay. Target cells of the WEHI-105.7 NZB thymoma (H-2 d, Mtab) were labeled with SlCr, washed four times, and incubated for 75 rain at 37~ with increasing concentrations of the indicated peptides in the presence of 50 nM fNDll-12. Target cells were washed twice before incubation with Mt ...
p-IRS-1/2 (Tyr 612)-R: sc-17195-R
... Insulin receptor substrate-1 (IRS-1) is a 170-185 kDa substrate of the insulin receptor that undergoes phosphorylation in response to insulin, IGF-1 and IL-4. tyrosine (Tyr) phosphorylation of IRS-1 mediates insulin-stimulated responses, while serine (Ser)/threonine (Thr) phosphorylation of IRS-1 ca ...
... Insulin receptor substrate-1 (IRS-1) is a 170-185 kDa substrate of the insulin receptor that undergoes phosphorylation in response to insulin, IGF-1 and IL-4. tyrosine (Tyr) phosphorylation of IRS-1 mediates insulin-stimulated responses, while serine (Ser)/threonine (Thr) phosphorylation of IRS-1 ca ...
Additional file 3
... studied in more detail. In particular, the search for putative binding sites on the protein surface is an important task as it provides clues to the functions of the C-type lectins, and also helps reduce the computational time required for subsequent docking studies and virtual screening. A typical ...
... studied in more detail. In particular, the search for putative binding sites on the protein surface is an important task as it provides clues to the functions of the C-type lectins, and also helps reduce the computational time required for subsequent docking studies and virtual screening. A typical ...
The structure and function of proline
... generally form extended structures and flexible regions that are hard to crystallize. For this reason, there are very few crystal structures of PRRs. Most structural information on such regions has come from solution-state n.m.r. and c.d. spectroscopy, and from modelling studies using secondary stru ...
... generally form extended structures and flexible regions that are hard to crystallize. For this reason, there are very few crystal structures of PRRs. Most structural information on such regions has come from solution-state n.m.r. and c.d. spectroscopy, and from modelling studies using secondary stru ...
compound having protonated forms of ethylenediaminetetraacetate
... became microbiologically contaminated, ethanol should be added, the solution covered with a plastic film, filtered without vacuum 2 or more days after, and left to evaporate as before. Using a molar ratio Cu(II)/ H4EDTA/AdeH 1:1:2, a pale blue powder of the type Cu(EDTA) ×/(AdeH2)2 ×/n H2O is also o ...
... became microbiologically contaminated, ethanol should be added, the solution covered with a plastic film, filtered without vacuum 2 or more days after, and left to evaporate as before. Using a molar ratio Cu(II)/ H4EDTA/AdeH 1:1:2, a pale blue powder of the type Cu(EDTA) ×/(AdeH2)2 ×/n H2O is also o ...
Part 2
... As shown ‘tissues’ and the blue squares marked ‘Hb’. Small white circles in must then enter the blue squares with the label ‘oxygen uptake’. Animation. These must then move down to the ‘tissues’ and majority of the circles must be released onto the tissues. When this happens, the red curve in the gr ...
... As shown ‘tissues’ and the blue squares marked ‘Hb’. Small white circles in must then enter the blue squares with the label ‘oxygen uptake’. Animation. These must then move down to the ‘tissues’ and majority of the circles must be released onto the tissues. When this happens, the red curve in the gr ...
SAMHD1 is a single-stranded nucleic acid
... until analysis. Protein concentrations were determined by absorbance measurements at 280 nm using the calculated ...
... until analysis. Protein concentrations were determined by absorbance measurements at 280 nm using the calculated ...
Docking QM/MM
... be seen from both microwave studies of common substituents such as aniline60 and from an analysis of small molecule structures reported in the CSD where hydrogen atom positions have been resolved. A search for aniline containing small molecule structures also reveals numerous examples of NH bonds de ...
... be seen from both microwave studies of common substituents such as aniline60 and from an analysis of small molecule structures reported in the CSD where hydrogen atom positions have been resolved. A search for aniline containing small molecule structures also reveals numerous examples of NH bonds de ...
ppt - Chair of Computational Biology
... Structural differences between cis and trans isomers Structural differences between the cis and trans Itk SH2 domain provide a basis for conformer-specific binding to the Itk SH3 domain. a, A backbone ribbon representation of the Itk cis SH2 domain with the Itk polyproline peptide (KPLPPTP shown in ...
... Structural differences between cis and trans isomers Structural differences between the cis and trans Itk SH2 domain provide a basis for conformer-specific binding to the Itk SH3 domain. a, A backbone ribbon representation of the Itk cis SH2 domain with the Itk polyproline peptide (KPLPPTP shown in ...
Lecture 2
... •Harder nucleophiles like alkoxide ion, R-O–, attack the acyl (carbonyl) carbon. •Softer nucleophiles like the cyanide ion, NC–, and the thioanion, R-S–, attack the "beta" alkyl carbon ...
... •Harder nucleophiles like alkoxide ion, R-O–, attack the acyl (carbonyl) carbon. •Softer nucleophiles like the cyanide ion, NC–, and the thioanion, R-S–, attack the "beta" alkyl carbon ...
Molecular Analysis of the Prostacyclin Receptor’s Interaction with
... lipid modification of CaaX-containing proteins involves the initial isoprenylation of the target Cys through carbon (C)-15 farnesylation or C-20 geranylgeranylation followed by proteolytic cleavage, or –aaXing, of the three terminal residues and subsequent carboxymethylation of the nascent terminal ...
... lipid modification of CaaX-containing proteins involves the initial isoprenylation of the target Cys through carbon (C)-15 farnesylation or C-20 geranylgeranylation followed by proteolytic cleavage, or –aaXing, of the three terminal residues and subsequent carboxymethylation of the nascent terminal ...
Large-scale analysis of Influenza A virus nucleoprotein sequence
... Small-molecule ligand binding potential The binding potential of NP for small molecule drugs was predicted based on clusters of energetically favourable interaction sites with a methyl-probe (Q-SiteFinder (Laurie and Jackson, 2005)) and docking of a library of small organic solvent molecules (FtMap ...
... Small-molecule ligand binding potential The binding potential of NP for small molecule drugs was predicted based on clusters of energetically favourable interaction sites with a methyl-probe (Q-SiteFinder (Laurie and Jackson, 2005)) and docking of a library of small organic solvent molecules (FtMap ...
ATP-binding site as a further application of neural network
... residues in catalytic sites of E. coli ATP synthase was shown to improve the catalytic activity by ten times [4,] while negatively charged residues were found to abrogate the Pi binding in the same catalytic site [7, 8]. Knowledge of specific amino acids involved in ATP binding plays crucial role in ...
... residues in catalytic sites of E. coli ATP synthase was shown to improve the catalytic activity by ten times [4,] while negatively charged residues were found to abrogate the Pi binding in the same catalytic site [7, 8]. Knowledge of specific amino acids involved in ATP binding plays crucial role in ...
Lipoidal permeability central to drug ADME
... unbound concentration in TBW. • Apparent accumulation is membrane binding in tissues. • PPB not important in vivo. • Less lipoidal permeable drugs can be excluded (brain) or accumulated (liver) in certain tissues • Lyosomal accumulation may not be active drug. ...
... unbound concentration in TBW. • Apparent accumulation is membrane binding in tissues. • PPB not important in vivo. • Less lipoidal permeable drugs can be excluded (brain) or accumulated (liver) in certain tissues • Lyosomal accumulation may not be active drug. ...
Slide 1 - Elsevier
... Figure 3-26 Genomic mechanism of activation by steroids. The interaction of steroids with different genes in a target cell may direct the synthesis of structural proteins such as cytoskeletal elements or receptors as well as enzymes. These enzymes may produce a variety of effects within the cell. T ...
... Figure 3-26 Genomic mechanism of activation by steroids. The interaction of steroids with different genes in a target cell may direct the synthesis of structural proteins such as cytoskeletal elements or receptors as well as enzymes. These enzymes may produce a variety of effects within the cell. T ...
SpectraMax M Series Multi-Mode Microplate Readers | Molecular
... filters to optimize detection levels and background. The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. ...
... filters to optimize detection levels and background. The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. ...
Erlanson et al. PNAS - UCSF Macromolecular Structure Group
... binding compounds would improve the success rate, but screening at high concentrations is generally impractical because of compound insolubility and assay artifacts. Moreover, the screening process does not target specific sites for drug design, only those sites for which a high-throughput assay is ...
... binding compounds would improve the success rate, but screening at high concentrations is generally impractical because of compound insolubility and assay artifacts. Moreover, the screening process does not target specific sites for drug design, only those sites for which a high-throughput assay is ...
Full Text
... The helices are reasonably well packed without gross spaces between them when studied as a space-filling model (Figure 1B). The crossing of the helices in a left-handed fourhelical bundle produces a funnel shaped cavity within the core of the four-helix bundle. This cavity has access to the outside ...
... The helices are reasonably well packed without gross spaces between them when studied as a space-filling model (Figure 1B). The crossing of the helices in a left-handed fourhelical bundle produces a funnel shaped cavity within the core of the four-helix bundle. This cavity has access to the outside ...