Secondary structure prediction method (SOPMA)
... Argus Lab is a molecular modeling program that runs on Windows 98, NT, and 2000. Argus Lab consists of a user interface that supports OpenGL graphics display of molecule structures and runs quantum mechanical calculations using the Argus compute server. The Argus compute server is constructed using ...
... Argus Lab is a molecular modeling program that runs on Windows 98, NT, and 2000. Argus Lab consists of a user interface that supports OpenGL graphics display of molecule structures and runs quantum mechanical calculations using the Argus compute server. The Argus compute server is constructed using ...
Enzymes – Enzyme Mechanism
... • Two catalytic modes based on binding properties can each increase reaction rates over 10,000-fold : (1) Proximity effect - collecting and positioning substrate molecules in the active site (2) Transition-state (TS) stabilization - transition states bind more tightly than substrates ...
... • Two catalytic modes based on binding properties can each increase reaction rates over 10,000-fold : (1) Proximity effect - collecting and positioning substrate molecules in the active site (2) Transition-state (TS) stabilization - transition states bind more tightly than substrates ...
1 Enzymes – Enzyme Mechanism
... provides substrate specificity and catalytic power • Two catalytic modes based on binding properties can each increase reaction rates over 10,000-fold : (1) Proximity effect - collecting and positioning substrate molecules in the active site ...
... provides substrate specificity and catalytic power • Two catalytic modes based on binding properties can each increase reaction rates over 10,000-fold : (1) Proximity effect - collecting and positioning substrate molecules in the active site ...
Receptors and Hormone Action
... or by influencing pre-existing repressor molecules, to increase initiation sites for RNA polymerase and increase transcription Take longer time (hours) than peptide hormones ...
... or by influencing pre-existing repressor molecules, to increase initiation sites for RNA polymerase and increase transcription Take longer time (hours) than peptide hormones ...
A tale of two functions: enzymatic activity and
... of 0.88 mM and 50 nM, respectively. The DNA was obtained by gel extraction following PCR using primers C46A5 and CTb3 and the accD gene as the template. After addition of glycerol to a final concentration of 2.5%, samples were resolved on 0.8% (w/v) agarose gels in 1 TAE (40 mM Tris–Cl at pH 8.0, 2 ...
... of 0.88 mM and 50 nM, respectively. The DNA was obtained by gel extraction following PCR using primers C46A5 and CTb3 and the accD gene as the template. After addition of glycerol to a final concentration of 2.5%, samples were resolved on 0.8% (w/v) agarose gels in 1 TAE (40 mM Tris–Cl at pH 8.0, 2 ...
Enzyme
... a straight line (Figure 8–7), where the slope of the line n is the Hill coefficient, • an empirical parameter whose value is a function of the number, kind, and strength of the interactions of the multiple substrate-binding sites on the enzyme. • When n = 1, all binding sites behave independently, a ...
... a straight line (Figure 8–7), where the slope of the line n is the Hill coefficient, • an empirical parameter whose value is a function of the number, kind, and strength of the interactions of the multiple substrate-binding sites on the enzyme. • When n = 1, all binding sites behave independently, a ...
Procainamide4
... • Diverse drugs activate through heterodimer complex • Protect against xenobiotics • Cause drug-drug interactions T.M. Wilson, S. A. Kliewer 2002:1, 259-266 ...
... • Diverse drugs activate through heterodimer complex • Protect against xenobiotics • Cause drug-drug interactions T.M. Wilson, S. A. Kliewer 2002:1, 259-266 ...
Highly Efficient Micro RNA Enrichment
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
Hemoglobin Learning Objective Hemoglobin
... Oxygen binding and structural changes a) Concerted model: The first model proposed by Monod, Wyman and Changeux, also known as the MWC model, assumes that each subunit can exist in at least two conformational states and hypothesizes that all subunits make the transition from one state to the other s ...
... Oxygen binding and structural changes a) Concerted model: The first model proposed by Monod, Wyman and Changeux, also known as the MWC model, assumes that each subunit can exist in at least two conformational states and hypothesizes that all subunits make the transition from one state to the other s ...
full text pdf
... the total serum protein. Its structure contains many hydrophobic pockets which bind a variety of biological molecules. Several examples follow: –– By binding to albumin, fatty acids can be transported at high concentrations (free fatty acids are soluble to about 10-6 M while the fatty acid - albumin ...
... the total serum protein. Its structure contains many hydrophobic pockets which bind a variety of biological molecules. Several examples follow: –– By binding to albumin, fatty acids can be transported at high concentrations (free fatty acids are soluble to about 10-6 M while the fatty acid - albumin ...
Structure and Properties of Hemoglobin Learning Objectives What
... The only exception to this are two histidine residues termed as proximal histidine (F8) and distal histidine (E7) They play indispensible role in heme pocket and function in oxygen binding Hemoglobin molecule can bind four O2 molecules (one per heme) Hb exhibits cooperative binding kinetics i.e. if ...
... The only exception to this are two histidine residues termed as proximal histidine (F8) and distal histidine (E7) They play indispensible role in heme pocket and function in oxygen binding Hemoglobin molecule can bind four O2 molecules (one per heme) Hb exhibits cooperative binding kinetics i.e. if ...
FES, Active FES, Active
... aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice. ...
... aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice. ...
Deorphanization of GPRC6A: a promiscuous L-α
... buffer was then replaced with 50 µl assay buffer containing 6 µM Fluo-4AM (Molecular Probes) and incubated for 1 h at room temperature in the dark. Finally, cells were washed three times with assay buffer without bovine serum albumin and then incubated with 150 µl of assay buffer without bovine seru ...
... buffer was then replaced with 50 µl assay buffer containing 6 µM Fluo-4AM (Molecular Probes) and incubated for 1 h at room temperature in the dark. Finally, cells were washed three times with assay buffer without bovine serum albumin and then incubated with 150 µl of assay buffer without bovine seru ...
H INHIBITORS OF HIV-1 REVERSE TRANSCRIPTASE Research Article
... Objective: In the present study, we have designed and synthesized 15 novel 3-(1,3-dioxo-3a,4-dihydro-1H-isoindol-2(3H,7H,7aH)-yl)-N-(substituted phenyl) propanamide 4(a-o) analogs. Methods: Docking studies for all the derivatives 4(a-o) were performed using molecular modeling software autodock 4.2. ...
... Objective: In the present study, we have designed and synthesized 15 novel 3-(1,3-dioxo-3a,4-dihydro-1H-isoindol-2(3H,7H,7aH)-yl)-N-(substituted phenyl) propanamide 4(a-o) analogs. Methods: Docking studies for all the derivatives 4(a-o) were performed using molecular modeling software autodock 4.2. ...
Supplementary Notes - Word file
... Biotinylated histone peptides were synthesized at Stanford PAN facility or purchased from Upstate Biotechnology. Antibodies used in study: H2A, H2B, H4, H3-diMeK9, H3triMeK9, H3-AcK14, H4-meK20, tubulin, HDAC1, SAP30, and Sin3a (Upstate Biotechnology); H3, H3-diMeK4, H3-triMeK4, and H4-AcK8 (Abcam); ...
... Biotinylated histone peptides were synthesized at Stanford PAN facility or purchased from Upstate Biotechnology. Antibodies used in study: H2A, H2B, H4, H3-diMeK9, H3triMeK9, H3-AcK14, H4-meK20, tubulin, HDAC1, SAP30, and Sin3a (Upstate Biotechnology); H3, H3-diMeK4, H3-triMeK4, and H4-AcK8 (Abcam); ...
TAR-RNA binding by HIV-1 Tat protein is
... presently known (15). These compounds are also called enantioor L-nucleic acids, since they contain, in place of the natural D-ribose, its enantiomer L-ribose. We (16) and others (17–22) have already evaluated their potential as antisense and antigene agents for the control of gene expression, but, ...
... presently known (15). These compounds are also called enantioor L-nucleic acids, since they contain, in place of the natural D-ribose, its enantiomer L-ribose. We (16) and others (17–22) have already evaluated their potential as antisense and antigene agents for the control of gene expression, but, ...
Complexation Reactions
... Palladium(II) tends to form complexes with coordination number 4. A compound has the composition PdCl2 · 3 NH3. (a) Write the formula for this compound that best shows the coordination structure. (b) When an aqueous solution of the compound is treated with excess AgNO3(aq), how many moles of AgCl(s) ...
... Palladium(II) tends to form complexes with coordination number 4. A compound has the composition PdCl2 · 3 NH3. (a) Write the formula for this compound that best shows the coordination structure. (b) When an aqueous solution of the compound is treated with excess AgNO3(aq), how many moles of AgCl(s) ...
Peer-reviewed Article PDF
... for protozoan cells proliferation and considered as essential nutrients for the protozoan parasites. For example, Neurospora crassa mutants lacking ODC activity cannot grow unless putrescine, spermidine or spermines were added to the growing media [12,13]. The protozoan pathogens, Trypanosomatids de ...
... for protozoan cells proliferation and considered as essential nutrients for the protozoan parasites. For example, Neurospora crassa mutants lacking ODC activity cannot grow unless putrescine, spermidine or spermines were added to the growing media [12,13]. The protozoan pathogens, Trypanosomatids de ...
Enzyme Assay for Glutathione S-Transferase Protocol
... Glutathione S Transferase (GST) is an enzyme involved in detoxification of a wide range of compounds and is involved in reducing free radical damage in red blood cells. The enzyme is easily purified by affinity chromatography and has been used as a fusion partner for many recombinant proteins. Ident ...
... Glutathione S Transferase (GST) is an enzyme involved in detoxification of a wide range of compounds and is involved in reducing free radical damage in red blood cells. The enzyme is easily purified by affinity chromatography and has been used as a fusion partner for many recombinant proteins. Ident ...
Extraction, Purification and Analysis of Anti cancer activity of Ricin
... this group. The present study involves extraction of ricin proteins from powdered castor seed using buffer of definite composition, purification of extracted sample of castor seed was done using Dialysis and Ion Exchange Chromatography. The purified samples were tested for their purification by runn ...
... this group. The present study involves extraction of ricin proteins from powdered castor seed using buffer of definite composition, purification of extracted sample of castor seed was done using Dialysis and Ion Exchange Chromatography. The purified samples were tested for their purification by runn ...
The ACT Domain: A Small Molecule Binding Domain
... is particularly interesting because it contains an ACT domain followed closely, within the same chain, by a ␣ fold that together forms a seven-stranded  sheet (19). Mutagenesis studies (20) indicate that the effector ligand (isoleucine) binding site is located at the interface between these two ...
... is particularly interesting because it contains an ACT domain followed closely, within the same chain, by a ␣ fold that together forms a seven-stranded  sheet (19). Mutagenesis studies (20) indicate that the effector ligand (isoleucine) binding site is located at the interface between these two ...
Preparing MagBeads for Sequencing
... subject to change without notice. Pacific Biosciences assumes no responsibility for any errors or omissions in this document. Certain notices, terms, conditions and/or use restrictions may pertain to your use of Pacific Biosciences products and/or third party products. Please refer to the applicable ...
... subject to change without notice. Pacific Biosciences assumes no responsibility for any errors or omissions in this document. Certain notices, terms, conditions and/or use restrictions may pertain to your use of Pacific Biosciences products and/or third party products. Please refer to the applicable ...
In the name of God
... law: green laser light in a solution of Rhodamine 6B. The beam intensity becomes weaker as it passes through solution ...
... law: green laser light in a solution of Rhodamine 6B. The beam intensity becomes weaker as it passes through solution ...