Structural characterization of an antibody in complex with
... delineate another pocket that the heavy chain CDR1 loop fits into. At the edge of the binding interface, PSA residue Lys239 interacts favorably with antibody light chain CDR1 loop residue L:Phe27D. Several hydrophobic and aromatic amino acids from the antibody heavy chain CDR3 loop and from PSA cont ...
... delineate another pocket that the heavy chain CDR1 loop fits into. At the edge of the binding interface, PSA residue Lys239 interacts favorably with antibody light chain CDR1 loop residue L:Phe27D. Several hydrophobic and aromatic amino acids from the antibody heavy chain CDR3 loop and from PSA cont ...
Chen-6-Translation
... Polysome In both prokaryotes and eukaryotes, mRNAs are read simultaneously by numerous ribosomes, An mRNA with several ribosomes bound to it is referred to as a polysome. ...
... Polysome In both prokaryotes and eukaryotes, mRNAs are read simultaneously by numerous ribosomes, An mRNA with several ribosomes bound to it is referred to as a polysome. ...
Unraveling the mechanisms of RNA
... affinity of the U1A/U1hpII interaction by more than 100fold. Measurements of the individual rate constants further showed that this loss of affinity was over-whelmingly due to a decrease in association rate. Although as expected, increasing salt concentration also reduced the association rates for the ...
... affinity of the U1A/U1hpII interaction by more than 100fold. Measurements of the individual rate constants further showed that this loss of affinity was over-whelmingly due to a decrease in association rate. Although as expected, increasing salt concentration also reduced the association rates for the ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... for evaluation to check the quality of the generated model. There was more than 62 % sequence identity with E value 0.00e-1 in query and template sequences. Then MODELLER was used to generate the three dimensional structure of PEPCK. It is found that template structure contains different ligand whic ...
... for evaluation to check the quality of the generated model. There was more than 62 % sequence identity with E value 0.00e-1 in query and template sequences. Then MODELLER was used to generate the three dimensional structure of PEPCK. It is found that template structure contains different ligand whic ...
Regulatory Strategies
... What are some of the characteristics of this form of regulation? – Activity influenced by non-covalent binding of metabolite called a modulator • May be inhibitory or stimulatory • May have one (monovalent) or several (polyvalent) ...
... What are some of the characteristics of this form of regulation? – Activity influenced by non-covalent binding of metabolite called a modulator • May be inhibitory or stimulatory • May have one (monovalent) or several (polyvalent) ...
Thermodynamic analysis of DNA binding by a Bacillus single
... data was also carried out (Figure 4 inset). The estimated KD value was 1.2 ± 0.8 x 10−9 M. In order to determine the correct method of analysis of binding data, we have examined the “Goodness of Fit” using both models. The R2 value for the sigmoidal dose–response model was found to be 0.98, whereas, ...
... data was also carried out (Figure 4 inset). The estimated KD value was 1.2 ± 0.8 x 10−9 M. In order to determine the correct method of analysis of binding data, we have examined the “Goodness of Fit” using both models. The R2 value for the sigmoidal dose–response model was found to be 0.98, whereas, ...
CD - Instituto de Investigaciones Biotecnológicas
... Radar - De novo repeat detection in protein sequences REP - Searches a protein sequence for repeats REPRO - De novo repeat detection in protein sequences TRUST - De novo repeat detection in protein sequences XSTREAM - De novo tandem repeat detection and architecture modeling in protein sequences SAP ...
... Radar - De novo repeat detection in protein sequences REP - Searches a protein sequence for repeats REPRO - De novo repeat detection in protein sequences TRUST - De novo repeat detection in protein sequences XSTREAM - De novo tandem repeat detection and architecture modeling in protein sequences SAP ...
Document
... Result: both heparin and repressor inhibits (re)association of polymerase with promoter. Analysis: (1) heparin binds polymerase preventing association with DNA (2) repressor does the same by binding to the operator adjacent to the promoter and blocking access to the promoter by RNA polymerase. ...
... Result: both heparin and repressor inhibits (re)association of polymerase with promoter. Analysis: (1) heparin binds polymerase preventing association with DNA (2) repressor does the same by binding to the operator adjacent to the promoter and blocking access to the promoter by RNA polymerase. ...
PRODUCTION, CHARACTERIZATION AND MOLECULAR DOCKING OF MYCOPHENOLIC ACID BYSSOCHLAMYS NIVEA Original Article
... active site by narrowing it. In the present study, the 3D structure of HCV and NS5B receptor (PDB ID 1QUV) that has been determined by X-ray crystallography was used for molecular docking studies. The 415th residue was replaced by phenylalanine and modeled prior to docking. The molecular docking was ...
... active site by narrowing it. In the present study, the 3D structure of HCV and NS5B receptor (PDB ID 1QUV) that has been determined by X-ray crystallography was used for molecular docking studies. The 415th residue was replaced by phenylalanine and modeled prior to docking. The molecular docking was ...
̶ Targets like an antibody ̶ Performs like a small molecule ̶
... Table 1: Bicycle shows no measurable binding (direct FP assay) up to 2μM against CAs other than CA IX (>666 fold selectivity). Acetazolamide on the other hand only shows 4–12 fold selectivity. This is understandable as the small number of residues that define the CA active site where acetazolamide b ...
... Table 1: Bicycle shows no measurable binding (direct FP assay) up to 2μM against CAs other than CA IX (>666 fold selectivity). Acetazolamide on the other hand only shows 4–12 fold selectivity. This is understandable as the small number of residues that define the CA active site where acetazolamide b ...
Combined fluorescence and electrochemical investigation on the
... acid did not cause any change in either the intensity or the wavelength. The titration curve can be interpreted by invoking a competitive binding model in which C8 binds to the same site as the probe DNSA. Since free DNSA does not produce fluorescence, displacement of the probe by C8 from HSA reduces ...
... acid did not cause any change in either the intensity or the wavelength. The titration curve can be interpreted by invoking a competitive binding model in which C8 binds to the same site as the probe DNSA. Since free DNSA does not produce fluorescence, displacement of the probe by C8 from HSA reduces ...
Bovine Peptidoglycan Recognition Protein
... essential to the Toll pathway in Drosophila. One family member, bovine PGN recognition protein-S (bPGRP-S), has been found to bind and kill microorganisms in a PGN-independent manner, raising questions about the identity of the bPGRP-S ligand. Addressing this, we have determined the binding and micr ...
... essential to the Toll pathway in Drosophila. One family member, bovine PGN recognition protein-S (bPGRP-S), has been found to bind and kill microorganisms in a PGN-independent manner, raising questions about the identity of the bPGRP-S ligand. Addressing this, we have determined the binding and micr ...
Strong and weak hydrogen bonds in the protein
... Among the limitations of our earlier study was the fact that the number of crystal structures examined was relatively small, only 28. Furthermore, several proteins in this group of structures are homologous to each other and so the number of truly independent observations is even smaller. We felt th ...
... Among the limitations of our earlier study was the fact that the number of crystal structures examined was relatively small, only 28. Furthermore, several proteins in this group of structures are homologous to each other and so the number of truly independent observations is even smaller. We felt th ...
The Endoplasmic Reticulum Glucosyltransferase
... The center of mass of fluorescence spectra of GCI2s revealed almost no variation of tryptophan exposure to the solvent as the chain grew from GCI2-(1–53) to GCI2-(1– 62), with an abrupt change upon the addition of the last two residues where the tryptophan side chain appears completely buried (Fig. ...
... The center of mass of fluorescence spectra of GCI2s revealed almost no variation of tryptophan exposure to the solvent as the chain grew from GCI2-(1–53) to GCI2-(1– 62), with an abrupt change upon the addition of the last two residues where the tryptophan side chain appears completely buried (Fig. ...
Environmentally Sensitive Fluorescent Sensors Based on Synthetic Peptides Linköping University Post Print
... straightforward and cost-effective and peptide-dye constructs can be manufactured at large scale. Moreover, synthetic peptides are robust, offer an even larger chemical versatility than recombinant proteins and are easily modified in a site-specific manner by means of orthogonal protection group str ...
... straightforward and cost-effective and peptide-dye constructs can be manufactured at large scale. Moreover, synthetic peptides are robust, offer an even larger chemical versatility than recombinant proteins and are easily modified in a site-specific manner by means of orthogonal protection group str ...
Activity of ribosomes and tmRNA of Streptomyces aureofaciens
... vegetative forms to aerial mycelium and spores. It has been shown that downshift in temperature is not sole inducer of cold shock response, because antibiotics that interfere with function of A-site of ribosome induce the response [1]. Bacterial cells evolved mechanisms to ensure quality control dur ...
... vegetative forms to aerial mycelium and spores. It has been shown that downshift in temperature is not sole inducer of cold shock response, because antibiotics that interfere with function of A-site of ribosome induce the response [1]. Bacterial cells evolved mechanisms to ensure quality control dur ...
Excerpt from J.Mol.Biol.
... structure. Side-chains for Leu248 and Leu249 are omitted for clarity. All potential hydrogen bonds to the pterin moiety are shown as dotted lines. The green model of BH4 illustrates its position in the binary Fe(II)·BH4 complex when superimposed on the ternary structure using conserved active-site r ...
... structure. Side-chains for Leu248 and Leu249 are omitted for clarity. All potential hydrogen bonds to the pterin moiety are shown as dotted lines. The green model of BH4 illustrates its position in the binary Fe(II)·BH4 complex when superimposed on the ternary structure using conserved active-site r ...
Chem*3560 Lecture 6: Allosteric regulation of enzymes
... ATP and CTP are allosteric effectors, substances that do not participate directly in the catalytic reaction, but which modulate the allosteric behaviour of the enzyme to regulate the reaction. When ATP and CTP are both present, they compete for the same binding site (see below), and the relative con ...
... ATP and CTP are allosteric effectors, substances that do not participate directly in the catalytic reaction, but which modulate the allosteric behaviour of the enzyme to regulate the reaction. When ATP and CTP are both present, they compete for the same binding site (see below), and the relative con ...
Probing the Role of a Conserved M1 Proline Residue in 5
... replaced by that of either Ala, or Gly, or Leu using conventional mutagenesis. Mutant mRNAs transcribed from the cDNA templates, when injected into X. laevis oocytes (at 50 ng/oocyte), failed to produce any detectable serotonin responses at ⫺60 mV holding potential with ⱕ1 mM serotonin. To determine ...
... replaced by that of either Ala, or Gly, or Leu using conventional mutagenesis. Mutant mRNAs transcribed from the cDNA templates, when injected into X. laevis oocytes (at 50 ng/oocyte), failed to produce any detectable serotonin responses at ⫺60 mV holding potential with ⱕ1 mM serotonin. To determine ...
Predicting drug-target interaction in cancers using homology
... DNA has been a major target for anticancer drugs. The effects of nucleic acid binding drugs are known for various diseases such as cancer. The different modes of drug binding to DNA include intercalation between adjacent base pairs, intrusion into the minor groove and into the major groove. In silic ...
... DNA has been a major target for anticancer drugs. The effects of nucleic acid binding drugs are known for various diseases such as cancer. The different modes of drug binding to DNA include intercalation between adjacent base pairs, intrusion into the minor groove and into the major groove. In silic ...
Microsoft Word
... stability is investigated in order to understand the basic mechanism of stabilization of casein, as a result of microenvironment changes brought about by these cosolvents. Effect of different cosolvents on the functional stability is investigated to optimize their performance at extremes of tempera ...
... stability is investigated in order to understand the basic mechanism of stabilization of casein, as a result of microenvironment changes brought about by these cosolvents. Effect of different cosolvents on the functional stability is investigated to optimize their performance at extremes of tempera ...
H - Departamento de Física Geral
... of the instrument. In this case the reference cell contains buffer only, and the sample cell contains the macromolecule dissolved in buffer. Both cells are ...
... of the instrument. In this case the reference cell contains buffer only, and the sample cell contains the macromolecule dissolved in buffer. Both cells are ...
INTERACTION STUDY OF CURCULIGOSIDE A AND ITS AGLYCONE AS CYCLOOXYGENASE
... Inflammation is part of the complex biological response of vascular tissues to harmful stimuli, such as pathogens, damaged cells, or irritants [1]. Inflammation is a basic way in which the body reacts to infection, irritation or other injury. The key features are redness, warmth, swelling and pain. ...
... Inflammation is part of the complex biological response of vascular tissues to harmful stimuli, such as pathogens, damaged cells, or irritants [1]. Inflammation is a basic way in which the body reacts to infection, irritation or other injury. The key features are redness, warmth, swelling and pain. ...
Exploration of binding site pattern in arachidonic
... Background: Cyclooxygenase (COXs) and Lipoxygenase (LOXs) pathways are the two major enzymatic pathways in arachidonic acid (AA) metabolism. The term eicosanoid is used to describe biologically active lipid mediators including prostaglandins, thromboxanes, leukotrienes and other oxygenated derivativ ...
... Background: Cyclooxygenase (COXs) and Lipoxygenase (LOXs) pathways are the two major enzymatic pathways in arachidonic acid (AA) metabolism. The term eicosanoid is used to describe biologically active lipid mediators including prostaglandins, thromboxanes, leukotrienes and other oxygenated derivativ ...
Transition metals and coordination chemistry
... The concept of coordination numbers was an important result of Werner's research. It clearly established the two most important aspects that are required to discuss chemical structure and change: oxidation state and coordination number. The concept of oxidation state implies that we have knowledge w ...
... The concept of coordination numbers was an important result of Werner's research. It clearly established the two most important aspects that are required to discuss chemical structure and change: oxidation state and coordination number. The concept of oxidation state implies that we have knowledge w ...