Structures of nucleotide-bound and free aIF2γ from Sulfolobus
... The nucleotide ring cavities in nucleotide-free and nucleotide-bound forms of Sso-aIF2γ have the same conformations, whereas the conformations of the P-loops are different (Fig. 2a). In the nucleotide-free form of Sso-aIF2γ the P-loop and switch 2 approach each other and the main chain carbonyl grou ...
... The nucleotide ring cavities in nucleotide-free and nucleotide-bound forms of Sso-aIF2γ have the same conformations, whereas the conformations of the P-loops are different (Fig. 2a). In the nucleotide-free form of Sso-aIF2γ the P-loop and switch 2 approach each other and the main chain carbonyl grou ...
In Vitro Characterization of Human Growth Hormone
... All mutants and wild type (wt) GH were produced in human embryonic kidney (HEK)-293 cells and an array of in vitro experiments was established for their characterization. It turned out that the novel Δ188-190 mutant is not a GHR antagonist after all. It has a diminished binding affinity to GHR, low ...
... All mutants and wild type (wt) GH were produced in human embryonic kidney (HEK)-293 cells and an array of in vitro experiments was established for their characterization. It turned out that the novel Δ188-190 mutant is not a GHR antagonist after all. It has a diminished binding affinity to GHR, low ...
Chemotaxis Assays for Marine and Freshwater Amoeba Jessica
... also benefit from this fitness advantage while living in such heterogeneous environments. One study on the soil-amoeba Dictyostelium discoideum suggested a strong chemotactic response towards folate, however the exact mechanisms by which this occurs remains unknown2. In fact, many questions remain i ...
... also benefit from this fitness advantage while living in such heterogeneous environments. One study on the soil-amoeba Dictyostelium discoideum suggested a strong chemotactic response towards folate, however the exact mechanisms by which this occurs remains unknown2. In fact, many questions remain i ...
SOMAmer® anti-Carbonic anhydrase 3 Reagent
... SOMAmer reagents have been qualified for target affinity capture in conjunction with numerous downstream applications. Specific protocols should be determined for the intended use. General application notes are available on our website (http://www.somalogic.com/resources/app-notes/). Specificity No ...
... SOMAmer reagents have been qualified for target affinity capture in conjunction with numerous downstream applications. Specific protocols should be determined for the intended use. General application notes are available on our website (http://www.somalogic.com/resources/app-notes/). Specificity No ...
Elucidating Substrate and Inhibitor Binding Sites on the Surface of
... A molecular understanding of substrate recognition of protein kinases provides an important basis for the development of substrate competitive inhibitors. Here, we explored substrate recognition and competitive inhibition of glycogen synthase kinase (GSK)-3β using molecular and computational tools. ...
... A molecular understanding of substrate recognition of protein kinases provides an important basis for the development of substrate competitive inhibitors. Here, we explored substrate recognition and competitive inhibition of glycogen synthase kinase (GSK)-3β using molecular and computational tools. ...
Molecular Docking
... The simple approach to tackle the protein flexibility problem is the 'soft docking' method It allows for slight penetrations between the receptor and the ligand molecules; this is a mathematical trick where the receptor and the ligand are held rigid and a 'soft' scoring function is used, allowing ...
... The simple approach to tackle the protein flexibility problem is the 'soft docking' method It allows for slight penetrations between the receptor and the ligand molecules; this is a mathematical trick where the receptor and the ligand are held rigid and a 'soft' scoring function is used, allowing ...
A: _____/18
... b) Drugs that are good inhibitors cannot by synthesized. c) The drugs are rapidly degraded. d) Virus particles with altered (mutant) proteases arise. ...
... b) Drugs that are good inhibitors cannot by synthesized. c) The drugs are rapidly degraded. d) Virus particles with altered (mutant) proteases arise. ...
Chapter 1
... characterizations require that the protein of interest is in a purified form and that ligand binding or transport can be measured. The work of Jan Knol has provided an elegant, reproducible method for the overexpression, purification and reconstitution of LacS into artificial lipid bilayers, yieldin ...
... characterizations require that the protein of interest is in a purified form and that ligand binding or transport can be measured. The work of Jan Knol has provided an elegant, reproducible method for the overexpression, purification and reconstitution of LacS into artificial lipid bilayers, yieldin ...
Structural Biochemistry/Enzyme/Active Site
... An active site is the part of an enzyme that directly binds to a substrate and carries a reaction. It contains catalytic groups which are amino acids that promote formation and degradation of bonds. By forming and breaking these bonds, enzyme and substrate interaction promotes the formation of the t ...
... An active site is the part of an enzyme that directly binds to a substrate and carries a reaction. It contains catalytic groups which are amino acids that promote formation and degradation of bonds. By forming and breaking these bonds, enzyme and substrate interaction promotes the formation of the t ...
Linking folding and binding
... a conformation closely approximating that of the bound form from the ensemble of conformations populated by the intrinsically disordered protein when free in solution. Clearly, any real system may utilize either one of these extreme mechanisms or some combination of the two; for example, it is to be ...
... a conformation closely approximating that of the bound form from the ensemble of conformations populated by the intrinsically disordered protein when free in solution. Clearly, any real system may utilize either one of these extreme mechanisms or some combination of the two; for example, it is to be ...
Fluorescence Polarization Detection of Brucella abortus
... anaerobic bacterium that is the causative agent for tuberculosis in cattle. Both of these agents have the ability to infect a number of other animals including humans. The presence of antibodies against these pathogens in bovine serum is indicative of infection. Fluorescence polarization (FP) is a f ...
... anaerobic bacterium that is the causative agent for tuberculosis in cattle. Both of these agents have the ability to infect a number of other animals including humans. The presence of antibodies against these pathogens in bovine serum is indicative of infection. Fluorescence polarization (FP) is a f ...
The transcriptional regulator CprK detects chlorination by combining
... (each with distinct DNA-binding affinities) are populated to significant levels [20]. By contrast, it has been proposed that CprK exhibits either extreme positive or no cooperativity, both effectively leading to an equilibrium between unbound and fully bound CprK [16]. In the latter case, this presu ...
... (each with distinct DNA-binding affinities) are populated to significant levels [20]. By contrast, it has been proposed that CprK exhibits either extreme positive or no cooperativity, both effectively leading to an equilibrium between unbound and fully bound CprK [16]. In the latter case, this presu ...
231. - Department of Chemistry
... In this study we report the completion of measurements of the kinetics and energetics of the ligation of (c-C5H5)Fe⫹ in He bath gas at 0.35 Torr with a variety of inorganic ligands containing hydrogen, carbon, nitrogen, and oxygen. Previous measurements in our laboratory have shown that the presence ...
... In this study we report the completion of measurements of the kinetics and energetics of the ligation of (c-C5H5)Fe⫹ in He bath gas at 0.35 Torr with a variety of inorganic ligands containing hydrogen, carbon, nitrogen, and oxygen. Previous measurements in our laboratory have shown that the presence ...
She2p Is a Novel RNA Binding Protein
... 30 m3, Tyson et al. [1979]) suggests that She2p is present at a cellular concentration of about 230 nM. As She2p is localized in yeast cells, the effective concentration is almost certainly higher. Although this calculation is unlikely to provide an accurate estimate of She2p concentration in cells ...
... 30 m3, Tyson et al. [1979]) suggests that She2p is present at a cellular concentration of about 230 nM. As She2p is localized in yeast cells, the effective concentration is almost certainly higher. Although this calculation is unlikely to provide an accurate estimate of She2p concentration in cells ...
GABA(A) Receptor Family
... neurons by interacting with the GABA(A) receptor on these cells1 There are two major types of GABA receptors: the ionotropic GABA(A) and the metabotropic GABA(B) receptors. GABA(A)receptor belongs to the ligand gated ion channel superfamily.1,2 It is a heteropentamer, with all of its five subunits c ...
... neurons by interacting with the GABA(A) receptor on these cells1 There are two major types of GABA receptors: the ionotropic GABA(A) and the metabotropic GABA(B) receptors. GABA(A)receptor belongs to the ligand gated ion channel superfamily.1,2 It is a heteropentamer, with all of its five subunits c ...
Case 26 The Role of Specific Amino Acids in the Peptide Hormone
... unable to carry out the signal transduction process. The glucagon antagonist would compete for binding with endogenous glucagon. If the antagonist bound instead of the endogenous glucagon, glycogenolysis would not occur. In order to construct a glucagon antagonist it is necessary to determine exactl ...
... unable to carry out the signal transduction process. The glucagon antagonist would compete for binding with endogenous glucagon. If the antagonist bound instead of the endogenous glucagon, glycogenolysis would not occur. In order to construct a glucagon antagonist it is necessary to determine exactl ...
Phospholipid signaling
... PC. PA can activate MAPK, CDPK, ion channels, and NADPH oxidase, all of which are involved in typical defencerelated responses. PA signalling is attenuated by its conversion to DGPP by PA kinase. All lipids or their derivatives that are involved in signalling are shown in red. Solid arrows indicate ...
... PC. PA can activate MAPK, CDPK, ion channels, and NADPH oxidase, all of which are involved in typical defencerelated responses. PA signalling is attenuated by its conversion to DGPP by PA kinase. All lipids or their derivatives that are involved in signalling are shown in red. Solid arrows indicate ...
No Slide Title
... Other evidence for lever arm model Spudich lab (1996): replace endogenous Dicteostelium myosin II gene with neck domain mutants - longer or shorter purify and measure velocity in motility assay velocity = step size/time bound to actin ...
... Other evidence for lever arm model Spudich lab (1996): replace endogenous Dicteostelium myosin II gene with neck domain mutants - longer or shorter purify and measure velocity in motility assay velocity = step size/time bound to actin ...
CLINICAL CHEMISTRY CHAPTER 5
... • Turbidimetry and Nephelometry – Light is obstructed by insoluble complexes ( usually antibody – antigen ) – Light is obstructed by these insoluble complexes ...
... • Turbidimetry and Nephelometry – Light is obstructed by insoluble complexes ( usually antibody – antigen ) – Light is obstructed by these insoluble complexes ...
Example of Research Proposal
... at the terminal residue are reported to be inactive as P-site substrates (6). Deletion of the 2'-OH removes a potentially important functional group that in the transition state may participate in hydrogen bonding, charge neutralization or proton transfer. Furthermore, the deoxy substitution results ...
... at the terminal residue are reported to be inactive as P-site substrates (6). Deletion of the 2'-OH removes a potentially important functional group that in the transition state may participate in hydrogen bonding, charge neutralization or proton transfer. Furthermore, the deoxy substitution results ...
Custom RT-qPCR Assays and Panels for any Human, Mouse, or Rat
... RealTime ready RT-qPCR Assays for any human, mouse, or rate gene of your choice are based on highly specific, LNA-substituted short hydrolysis probes from the Universal ProbeLibrary (UPL). Rigorously tested on the LightCycler® 480 Instrument with species-specific universal biological samples, each a ...
... RealTime ready RT-qPCR Assays for any human, mouse, or rate gene of your choice are based on highly specific, LNA-substituted short hydrolysis probes from the Universal ProbeLibrary (UPL). Rigorously tested on the LightCycler® 480 Instrument with species-specific universal biological samples, each a ...
Cloning, Characterization, and Expression of a G-Protein
... GRL104 was expressed in stably transfected CHO-K1 cells, and the peptide ligand was identified by the novel use of a multistep HPLC approach, together with a functional calcium assay. The peptide sequence was deduced to be PSFHSWSamide. When this peptide was analyzed for sequence homology against a ...
... GRL104 was expressed in stably transfected CHO-K1 cells, and the peptide ligand was identified by the novel use of a multistep HPLC approach, together with a functional calcium assay. The peptide sequence was deduced to be PSFHSWSamide. When this peptide was analyzed for sequence homology against a ...
UMCG
... Antitrypanosomiasis drug development based on structures of glycolytic enzymes. In Structure-Based Drug Design (Veerapandian, P., ed.), pp. 365-394. Marcel Dekker, New York. ...
... Antitrypanosomiasis drug development based on structures of glycolytic enzymes. In Structure-Based Drug Design (Veerapandian, P., ed.), pp. 365-394. Marcel Dekker, New York. ...
IN SILICO SCREENING OF PHYTOCHEMICAL COMPOUNDS TARGETING CHILDHOOD ABSENCE EPILEPSY (CAE)
... server [9], since the identified templates had very low percentage identity (20%). Since, the identity is less than 30%, hence it is hard to identify the best template and generate accurate sequence-template alignments [10, 11]. The diseased and normal protein models (Table. 3) were analyzed by usin ...
... server [9], since the identified templates had very low percentage identity (20%). Since, the identity is less than 30%, hence it is hard to identify the best template and generate accurate sequence-template alignments [10, 11]. The diseased and normal protein models (Table. 3) were analyzed by usin ...