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SOMAmer® anti-Carbonic anhydrase 3 Reagent DESCRIPTION SOMAmer Reagent No. Protein Target Target UniProt ID Species Reactivity 5’ Functional Mod. Target Source Formulation B-3799-11_2 Carbonic anhydrase 3 P07451 Human Biotinylated Synthetic Oligonucleotide R&D Systems®, PN 2185-CA-050 Buffered SOMAmer reagent delivered at 10 μM in 5 mM HEPES, 1 mM EDTA, pH 8 ACTIVITY SOMAmer reagents have been qualified for target affinity capture in conjunction with numerous downstream applications. Specific protocols should be determined for the intended use. General application notes are available on our website (http://www.somalogic.com/resources/app-notes/). Specificity No binding was observed with Carbonic anhydrase I, Carbonic anhydrase II, Carbonic anhydrase XIII, Carbonic anhydrase VII, Carbonic anhydrase 6, Carbonic anhydrase X, or Carbonic anhydrase 9. Affinity The Kd for Carbonic anhydrase 3 binding to the SOMAmer reagent, measured -9 by Zorbax Affinity method, is typically 7 x 10 M. See Certificate of Analysis for details. HANDLING and STORAGE Handling Shipping Stability & Storage Heat/cool treatment on material ready for use (not stocks) is recommended (e.g. heat the reagent to 95 °C for 5 minutes, then cool to assay temperature). The product is shipped frozen. Upon receipt, store it immediately at the temperature recommended below. For long term use, aliquotting is recommended to avoid freeze-thaw cycles. 2 years from date of receipt at -20 °C to -70 °C, as supplied 3 months at 4 °C TARGET FUNCTION Reversible hydration of carbon dioxide. (Source: The UniProt Consortium, Activities at the Universal Protein Resource (UniProt), Nucleic Acids Res. 42: D191-D198 (2014).) SomaLogic, Inc. • 2945 Wilderness Place Boulder, CO 80301 • www.somalogic.com Technical Support 800-324-0783 • Main Number 303-625-9000 570-00006, Rev 0, DCN 15-326 Effective Date: 10/5/2015 2015 SomaLogic, Inc.