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... assignable for protons Hd and Ha respectively. The two doublets of doublet at δ 6.5 (JHa-Hb = JHb-Hc = J = 8.0 Hz) and 6.9 (JHb-Hc= JHc-Hd = J = 8.0 Hz) accounts for the Ha and Hd respectively, while the relatively downfield signal at δ 8.5 has been assigned for the imine (=N-H) proton of 2-mercapto ...
... assignable for protons Hd and Ha respectively. The two doublets of doublet at δ 6.5 (JHa-Hb = JHb-Hc = J = 8.0 Hz) and 6.9 (JHb-Hc= JHc-Hd = J = 8.0 Hz) accounts for the Ha and Hd respectively, while the relatively downfield signal at δ 8.5 has been assigned for the imine (=N-H) proton of 2-mercapto ...
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... and acidic domains that have been shown in other species to act as activation domains [20]. Phylogenetic analysis (Fig. 1) indicated that the LeERFs could be assigned to previously described ERF classes [8]. LeERF1 belongs to the highly conserved ERF class I. LeERF3 belongs to the class II putative ...
... and acidic domains that have been shown in other species to act as activation domains [20]. Phylogenetic analysis (Fig. 1) indicated that the LeERFs could be assigned to previously described ERF classes [8]. LeERF1 belongs to the highly conserved ERF class I. LeERF3 belongs to the class II putative ...
Recombinant Human Heparan Sulfate 3-O
... Universal Sulfotransferase Activity Kit (Catalog # EA003) 10X Assay Buffer (supplied in kit): 500 mM Tris, 150 mM MgCl2, pH 7.5 Recombinant Human Heparan Sulfate3OSulfotransferase 1/HS3ST1 (rhHS3ST1) (Catalog # 5968ST) Donor Substrate: PAP32S (3'Phosphoadenosine5'Phosphosulfate) (Catalog # ...
... Universal Sulfotransferase Activity Kit (Catalog # EA003) 10X Assay Buffer (supplied in kit): 500 mM Tris, 150 mM MgCl2, pH 7.5 Recombinant Human Heparan Sulfate3OSulfotransferase 1/HS3ST1 (rhHS3ST1) (Catalog # 5968ST) Donor Substrate: PAP32S (3'Phosphoadenosine5'Phosphosulfate) (Catalog # ...
Electrostatics -- basic concepts
... It depends on the salt... Partitioning of ions between water and nonspecific sites on biomolecule Dependent on ion type (solvation energy, etc.) Dominate at high salt concentrations Functional behavior: protein stability, membrane ...
... It depends on the salt... Partitioning of ions between water and nonspecific sites on biomolecule Dependent on ion type (solvation energy, etc.) Dominate at high salt concentrations Functional behavior: protein stability, membrane ...
Dissecting the Molecular Origins of Specific Protein
... containing a recognition sequence, which can be specifically bound by the protein. After equilibration at elevated pressure, protein-DNA complexes are separated from the free DNA probe by electrophoresis in the polyacrylamide gel. The resolved species are visualized and the stoichiometries of each s ...
... containing a recognition sequence, which can be specifically bound by the protein. After equilibration at elevated pressure, protein-DNA complexes are separated from the free DNA probe by electrophoresis in the polyacrylamide gel. The resolved species are visualized and the stoichiometries of each s ...
Lecture 4| Enzyme Catalysis: Structural basis and energetics of
... Effect of pH on enzyme catalysis • When the rate-‐limi(ng component of the cataly(c process involves a (tratable residue, the measured ac(vity of the enzyme depends on pH and the ioniza(on status of ...
... Effect of pH on enzyme catalysis • When the rate-‐limi(ng component of the cataly(c process involves a (tratable residue, the measured ac(vity of the enzyme depends on pH and the ioniza(on status of ...
VIRTUAL SCREENING OF POTENTIAL DRUG-LIKE INHIBITORS FROM MEDICINAL PLANTS
... Dr.Duke’s phytochemical and ethanobotanical databases. The screening of phytochemicals from the database in specific to antiviral activity and anticancer activity is done because HBXIP is an oncogenic and Hepatitis B virus interacting in nature. There were about many repeated compounds present in th ...
... Dr.Duke’s phytochemical and ethanobotanical databases. The screening of phytochemicals from the database in specific to antiviral activity and anticancer activity is done because HBXIP is an oncogenic and Hepatitis B virus interacting in nature. There were about many repeated compounds present in th ...
Antigenic structure of foot-and-mouth disease virus
... The icosahedral symmetry of F M D virions imposes restrictions on the bivalent binding of antibodies. It is interesting t o examine some possibilities for intra-virion binding of antibodies. The relationship of the pentameric subunits is illustrated in Fig. 1 and the viral proteins V P 1 , 2 and 3 h ...
... The icosahedral symmetry of F M D virions imposes restrictions on the bivalent binding of antibodies. It is interesting t o examine some possibilities for intra-virion binding of antibodies. The relationship of the pentameric subunits is illustrated in Fig. 1 and the viral proteins V P 1 , 2 and 3 h ...
Beili B24 25 Insulin
... Insulin. An aliquot of 1-6 micrograms of plasma membrane fragments prepared from cells over-expressing the human insulin receptor and 1 mg/well polyethyleneiminetreated wheat germ agglutinin type A scintillation proximity assay beads (Amersham Biosciences, Piscataway, NJ) were added to each well. Th ...
... Insulin. An aliquot of 1-6 micrograms of plasma membrane fragments prepared from cells over-expressing the human insulin receptor and 1 mg/well polyethyleneiminetreated wheat germ agglutinin type A scintillation proximity assay beads (Amersham Biosciences, Piscataway, NJ) were added to each well. Th ...
bioinorganic 1
... •basic studies of simple coordination compounds as models for metal in ‘real’ biological systems •studies on metal-containing proteins themselves •studies on naturally occurring metals – their presence and their roles/functions (i.e. answering questions like how do the metals get there? What do they ...
... •basic studies of simple coordination compounds as models for metal in ‘real’ biological systems •studies on metal-containing proteins themselves •studies on naturally occurring metals – their presence and their roles/functions (i.e. answering questions like how do the metals get there? What do they ...
Quiz - Columbus Labs
... Graphs of allosteric effects for a tetramer (n = 4) in terms of Y, the saturation function, versus [S]. Y is defined as [ligand-binding sites that are occupied by ligand]/[ total ligandbinding sites]. c = KR/KT. (substrate dissociation constant for the two states; when c = 0, KT is infinite, T doesn ...
... Graphs of allosteric effects for a tetramer (n = 4) in terms of Y, the saturation function, versus [S]. Y is defined as [ligand-binding sites that are occupied by ligand]/[ total ligandbinding sites]. c = KR/KT. (substrate dissociation constant for the two states; when c = 0, KT is infinite, T doesn ...
A New Way to Test the Free Amino Nitrogen Content
... Standard Brewing quality control analyses include the test for free amino nitrogen (FAN). This allows an estimation of the protein-content and is a beer quality indicator. The traditional way to determine the protein content of beers is based on Kjeldahl assays which are elaborate and quite expensiv ...
... Standard Brewing quality control analyses include the test for free amino nitrogen (FAN). This allows an estimation of the protein-content and is a beer quality indicator. The traditional way to determine the protein content of beers is based on Kjeldahl assays which are elaborate and quite expensiv ...
Human epidermal growth factor receptor (HER-1:HER
... medium was replaced with DMEM containing 1% FBS (low IgG; Life Technologies, Inc./Invitrogen). First conditioned medium was collected 4 d after transient transfection, followed by feeding and a second collection of the medium. Suspension cell protein expression was done using HEK 293T cells that wer ...
... medium was replaced with DMEM containing 1% FBS (low IgG; Life Technologies, Inc./Invitrogen). First conditioned medium was collected 4 d after transient transfection, followed by feeding and a second collection of the medium. Suspension cell protein expression was done using HEK 293T cells that wer ...
Protein Analysis-Determination of Protein Concentration
... When purifying a protein, we need to know how much protein is present in our samples. An assay is used to measure the concentration or amount of a substance. A protein assay, therefore, measures the concentration or amount of a protein. A large number of assays of protein concentration have been dev ...
... When purifying a protein, we need to know how much protein is present in our samples. An assay is used to measure the concentration or amount of a substance. A protein assay, therefore, measures the concentration or amount of a protein. A large number of assays of protein concentration have been dev ...
MolecularModelingDru..
... It is an effective painkiller. It reduces fever and inflammation when the body gets overzealous in its defenses against infection and damage. It slows blood clotting, reducing the chance of stroke and heart attack in susceptible individuals. It may be an effective addition to the fight against cance ...
... It is an effective painkiller. It reduces fever and inflammation when the body gets overzealous in its defenses against infection and damage. It slows blood clotting, reducing the chance of stroke and heart attack in susceptible individuals. It may be an effective addition to the fight against cance ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
... haemostasis, thrombosis, inflammation and tissue repair. (13,20) PARs also participate in progression of specific types of cancers.(21,22) In contrast to typical receptor-agonist binding interaction, the interaction of PAR-1 with its activator, thrombin, is that of a protease substrate and dynamic a ...
... haemostasis, thrombosis, inflammation and tissue repair. (13,20) PARs also participate in progression of specific types of cancers.(21,22) In contrast to typical receptor-agonist binding interaction, the interaction of PAR-1 with its activator, thrombin, is that of a protease substrate and dynamic a ...
LynchSpr15
... to determine the specific residue critical for antibody binding and whether analogous side chain characteristics in the derivative sequences would enhance binding. The STD NMR results indicated that Pro6 is a critical residue for binding as it displayed greater saturation transfer effects for its si ...
... to determine the specific residue critical for antibody binding and whether analogous side chain characteristics in the derivative sequences would enhance binding. The STD NMR results indicated that Pro6 is a critical residue for binding as it displayed greater saturation transfer effects for its si ...
patrick_ch19_p1
... • Easily hydrolysed in stomach (acid catalysed hydrolysis) • Easily hydrolysed in blood (esterases) • No selectivity between receptor types • No selectivity between different target organs ...
... • Easily hydrolysed in stomach (acid catalysed hydrolysis) • Easily hydrolysed in blood (esterases) • No selectivity between receptor types • No selectivity between different target organs ...
INFLUENCE OF WATER - ETHANOL SOLVENT ON
... pK value on 0. 86 and increase pKH2 on 0. 94 logarithmic unit. In the first case process corresponds to the mechanism of dissociation of cationic acids, and in the second - the uncharged. The increase of ethanol concentration results to the considerable increase of stability constants of L-tyrosine ...
... pK value on 0. 86 and increase pKH2 on 0. 94 logarithmic unit. In the first case process corresponds to the mechanism of dissociation of cationic acids, and in the second - the uncharged. The increase of ethanol concentration results to the considerable increase of stability constants of L-tyrosine ...
Use of Heparin HyperD® M Affinity Resin for Batch Mode Protein
... 9. Wash resin with 4x 250 µL (20x resin volume) equilibration buffer. 10. Retained proteins are eluted with a binding buffer with progressively increasing salt concentration. Two times 150 µL of each elution buffer is used (= 3 resin volumes) to ensure good elution efficiency and to minimize sample ...
... 9. Wash resin with 4x 250 µL (20x resin volume) equilibration buffer. 10. Retained proteins are eluted with a binding buffer with progressively increasing salt concentration. Two times 150 µL of each elution buffer is used (= 3 resin volumes) to ensure good elution efficiency and to minimize sample ...
Structural and functional studies on C4b
... 25 000 Mr fragment was produced after the initial cleavage by chymotrypsin. As shown in Fig. 4 (a and b), the liberation of the 48 000 Mr fragment from the disulphide-linked core fragments correlated with the further spfitting of the 48 000 Mr and 25 000 Mr fragments. Since chymotryptic digestion pr ...
... 25 000 Mr fragment was produced after the initial cleavage by chymotrypsin. As shown in Fig. 4 (a and b), the liberation of the 48 000 Mr fragment from the disulphide-linked core fragments correlated with the further spfitting of the 48 000 Mr and 25 000 Mr fragments. Since chymotryptic digestion pr ...
Hemoglobin a hemoglobinpatie
... • The binding of protons H+ by Hb lowers its affinity for O2 • Increasing pH, that is, removing protons,stimulates Hb to bind O2 • pH of the blood decreases as it enters tissues because CO2 produced by metabolism is converted to H2CO3 • Dissociation of H2CO3 produces protons • Promote the release of ...
... • The binding of protons H+ by Hb lowers its affinity for O2 • Increasing pH, that is, removing protons,stimulates Hb to bind O2 • pH of the blood decreases as it enters tissues because CO2 produced by metabolism is converted to H2CO3 • Dissociation of H2CO3 produces protons • Promote the release of ...
Steroid hormone receptor homologs in development
... Similarly, ecdysteroids are required for insect metamorphosis, allowing the various molts into adulthood (Ashburner, 1971). An initial insight into how small, relatively simple molecules elicit such a diversity of complex responses was provided by the identification of steroid and thyroid hormone re ...
... Similarly, ecdysteroids are required for insect metamorphosis, allowing the various molts into adulthood (Ashburner, 1971). An initial insight into how small, relatively simple molecules elicit such a diversity of complex responses was provided by the identification of steroid and thyroid hormone re ...
Genome evolution: a sequence
... • Now estimate the number of substitution between pairs of 8-mers, compare this number to the number expected by the background model • Do it for a lot of sequence, so that statistics on the difference between observed and expected substitutions can be derived ...
... • Now estimate the number of substitution between pairs of 8-mers, compare this number to the number expected by the background model • Do it for a lot of sequence, so that statistics on the difference between observed and expected substitutions can be derived ...
INSILICO MODELING OF CAPSULAR POLYSACCHARIDE BIOSYNTHESIS PROTEIN STREPTOCOCCUS PNEUMONIAE LIGAND IDENTIFICATION
... module of Discovery Studio under CHARMm force field and the energy function is based on pairwise structural analysis between the nonbonding interactions of protein-ligand complex [14,15]. Finally, ADMET properties of ligands were studied through DISCOVERY STUDIO[16,17]. RESULTS AND DISCUSSION Homolo ...
... module of Discovery Studio under CHARMm force field and the energy function is based on pairwise structural analysis between the nonbonding interactions of protein-ligand complex [14,15]. Finally, ADMET properties of ligands were studied through DISCOVERY STUDIO[16,17]. RESULTS AND DISCUSSION Homolo ...