Frequency Domain Optical Coherence Tomography (FDOCT)
... IMSURE Summer Research Fellow At Beckman Laser Institute University of California at Irvine Irvine, CA 92612 Email: [email protected] Faculty mentor: Prof. Zhongping Chen Lab mentor: Jun Zhang ...
... IMSURE Summer Research Fellow At Beckman Laser Institute University of California at Irvine Irvine, CA 92612 Email: [email protected] Faculty mentor: Prof. Zhongping Chen Lab mentor: Jun Zhang ...
A method for characterizing longitudinal chromatic aberration of
... designed. It is particularly important, for example, that the source and detector pinholes are correctly positioned such that their images, in object space, lie exactly on top of each other with no relative spatial displacement. If this is achieved a very sharp effective point spread function with ...
... designed. It is particularly important, for example, that the source and detector pinholes are correctly positioned such that their images, in object space, lie exactly on top of each other with no relative spatial displacement. If this is achieved a very sharp effective point spread function with ...
Microscope
... object. The total magnifying power is determined by multiplying the power of the objective lens by the power of the ocular lens. Resolution is a measure of the clarity of the image; it is the minimum distance two points can be separated and still be distinguished as two points. The function of the m ...
... object. The total magnifying power is determined by multiplying the power of the objective lens by the power of the ocular lens. Resolution is a measure of the clarity of the image; it is the minimum distance two points can be separated and still be distinguished as two points. The function of the m ...
Optical, Confocal, and 4Pi Microscopy
... Optical microscopy has been in existence for many centuries. Since the viewing of the first cell, man has continually attempted to view smaller and smaller objects. In 1873, Ernst Abbe first discovered that diffraction limited the resolution of the optical microscope. And even with the advances in c ...
... Optical microscopy has been in existence for many centuries. Since the viewing of the first cell, man has continually attempted to view smaller and smaller objects. In 1873, Ernst Abbe first discovered that diffraction limited the resolution of the optical microscope. And even with the advances in c ...
A Brief History of the Microscope and its Significance
... reinvention of technical advances in disparate fields, and these processes continued to occur throughout the development of confocal and multiphoton excitation microscopy. Inventions were patented, patents were contested, and the process continues into the present. The prerequisites for the developm ...
... reinvention of technical advances in disparate fields, and these processes continued to occur throughout the development of confocal and multiphoton excitation microscopy. Inventions were patented, patents were contested, and the process continues into the present. The prerequisites for the developm ...
Development of the Optical Microscope
... process is caused by light being slowed as it passes through a different medium. Building on Abbe’s work, the Dutch scientist Frits Zernicke developed a brightfield microscope just before the Second World War in 1938 that measured phase shifts as illuminating light passed though the sample. At the t ...
... process is caused by light being slowed as it passes through a different medium. Building on Abbe’s work, the Dutch scientist Frits Zernicke developed a brightfield microscope just before the Second World War in 1938 that measured phase shifts as illuminating light passed though the sample. At the t ...
Contactless visible light probing for nanoscale ICs through 10 μm
... Abstract: This paper explains why only optical techniques will be able to provide debug and diagnosis of bulk silicon FinFET technologies. In order to apply optical techniques through a convenient thickness of silicon on the one hand, light is limited to NIR to minimize absorption. To match resoluti ...
... Abstract: This paper explains why only optical techniques will be able to provide debug and diagnosis of bulk silicon FinFET technologies. In order to apply optical techniques through a convenient thickness of silicon on the one hand, light is limited to NIR to minimize absorption. To match resoluti ...
Microscopes:
... Condenser – provides an even cone of light that illuminates the specimen. Light from the condenser converges at the plane of the specimen, passes through it, and then diverges to form an inverted illuminated cone which is captured by the objective lens. The most common condenser used is the Abbe. H ...
... Condenser – provides an even cone of light that illuminates the specimen. Light from the condenser converges at the plane of the specimen, passes through it, and then diverges to form an inverted illuminated cone which is captured by the objective lens. The most common condenser used is the Abbe. H ...
Microscopy Basics
... orders of diffraction maxima can enter the objective of the same NA than with axial illumination Improved resolution However, less light enters the objective worse contrast ...
... orders of diffraction maxima can enter the objective of the same NA than with axial illumination Improved resolution However, less light enters the objective worse contrast ...
Supplementary Infomation (doc 52K)
... channel signal intensities could be used to compare the levels of DHE probe in the uninjured and axotomized retinas. Determination of autophagy induction in GFP-LC3 retinas using confocal microscopy Autophagosomes were identified as green dots in the cytosol of RGCs using confocal microscopy with a ...
... channel signal intensities could be used to compare the levels of DHE probe in the uninjured and axotomized retinas. Determination of autophagy induction in GFP-LC3 retinas using confocal microscopy Autophagosomes were identified as green dots in the cytosol of RGCs using confocal microscopy with a ...
Fundamentals of Embryo Development uncovered in real time
... sCMOS camera to study the endoderm of the Zebrafish during early stages of development. Light sheet microscopy, such as SPIM has been shown to be a valuable technology that performs especially well in samples that are too large for conventional microscopy, such as confocal. In SPIM, instantaneous op ...
... sCMOS camera to study the endoderm of the Zebrafish during early stages of development. Light sheet microscopy, such as SPIM has been shown to be a valuable technology that performs especially well in samples that are too large for conventional microscopy, such as confocal. In SPIM, instantaneous op ...
The Role of Laboratory Instructors
... 1. Complete, or respond to, the following questions: a. The _____ is a lens under the stage that concentrates the light: condensor b. The area of a slide seen when looking through a microscope is the: field of view c. If the stage has a device that moves the slide from side to side and up or down, t ...
... 1. Complete, or respond to, the following questions: a. The _____ is a lens under the stage that concentrates the light: condensor b. The area of a slide seen when looking through a microscope is the: field of view c. If the stage has a device that moves the slide from side to side and up or down, t ...
3 Microscopy
... raster scan of the specimen, line by line, and recording the probe-surface interaction as a function of position. A Raster scan, or raster scanning, is the pattern of image detection and reconstruction in television, and is the pattern of image storage and transmission used in most computer image sy ...
... raster scan of the specimen, line by line, and recording the probe-surface interaction as a function of position. A Raster scan, or raster scanning, is the pattern of image detection and reconstruction in television, and is the pattern of image storage and transmission used in most computer image sy ...
Conroy2005-SurfaceMetrology.pdf
... according to a user programmed scan length, speed and stylus force. The stylus is mechanically coupled to the core of an LVDT (Linear Variable Differential Transformer). As the stage moves the sample, the stylus rides over the sample surface. Surface variations cause the stylus to be translated vert ...
... according to a user programmed scan length, speed and stylus force. The stylus is mechanically coupled to the core of an LVDT (Linear Variable Differential Transformer). As the stage moves the sample, the stylus rides over the sample surface. Surface variations cause the stylus to be translated vert ...
Microscopy - u.arizona.edu
... b. Light Source and Diaphragm A. Need consistent source of light; Diaphragm regulates amount of light – helps with contrast B. Light source can also be filtered, typically to blue-violet, why? c. Stage and Focusing Knobs A. Knobs move stage, which in turn moves slide, which in turn moves object – go ...
... b. Light Source and Diaphragm A. Need consistent source of light; Diaphragm regulates amount of light – helps with contrast B. Light source can also be filtered, typically to blue-violet, why? c. Stage and Focusing Knobs A. Knobs move stage, which in turn moves slide, which in turn moves object – go ...
Lecture 1. Introduction. Nature of light, geometric optics.
... Newer: CF lens – meaning Chromatic aberration Free. ...
... Newer: CF lens – meaning Chromatic aberration Free. ...
Stereoscopic microscopes
... Report accidents, such as a spilled culture or a cut, to the laboratory instructor. Our interest is ...
... Report accidents, such as a spilled culture or a cut, to the laboratory instructor. Our interest is ...
Cell Signaling Website Slides_10_4_11
... BIT 495/595: Cellular Signaling Techniques Overview: • Par9cipants will be introduced to a variety of methods for studying cellular signaling processes including theory, applica9ons and limita9ons. • Students wil ...
... BIT 495/595: Cellular Signaling Techniques Overview: • Par9cipants will be introduced to a variety of methods for studying cellular signaling processes including theory, applica9ons and limita9ons. • Students wil ...
Chapter 7 PP
... 0.1mm, 100µm, 100,000.0nm. Light Microscope 0.0002mm 0.2µm, 200.0nm 2000.0Å S.E.M. 0.002µm 2.0nm 20.0Å T.E.M. 0.0001µm 0.1nm. 1.0Å wavelength of light wavelength of electrons ...
... 0.1mm, 100µm, 100,000.0nm. Light Microscope 0.0002mm 0.2µm, 200.0nm 2000.0Å S.E.M. 0.002µm 2.0nm 20.0Å T.E.M. 0.0001µm 0.1nm. 1.0Å wavelength of light wavelength of electrons ...
sees double - TU Delft Repositories
... the spread of cancer cells through the body. “This sort of microscopy can help unravel the basics of the molecular machinery inside a cell,” says Prof. Houtsmuller, a Rotterdam cellular anatomist who has recently begun taking an interest in the vaguely foot-like membranes used by some cells to “sens ...
... the spread of cancer cells through the body. “This sort of microscopy can help unravel the basics of the molecular machinery inside a cell,” says Prof. Houtsmuller, a Rotterdam cellular anatomist who has recently begun taking an interest in the vaguely foot-like membranes used by some cells to “sens ...
Imaging Services
... sensitivity that were only possible under in vitro conditions just a few years ago. The newly developed and commercialized imaging instruments can provide highly sensitive capabilities for dynamic imaging of targeted tissue with high spatial resolution enabling investigators to monitor various chang ...
... sensitivity that were only possible under in vitro conditions just a few years ago. The newly developed and commercialized imaging instruments can provide highly sensitive capabilities for dynamic imaging of targeted tissue with high spatial resolution enabling investigators to monitor various chang ...
The Microscope
... that in the said matter there were many very little living animalcules, very prettily a-moving. The biggest sort had a very strong and swift motion, and shot through the water like a pike does through the water; mostly these were of small numbers." Actually he estimated more bacteria in one single d ...
... that in the said matter there were many very little living animalcules, very prettily a-moving. The biggest sort had a very strong and swift motion, and shot through the water like a pike does through the water; mostly these were of small numbers." Actually he estimated more bacteria in one single d ...
Theory of confocal fluorescence imaging in the
... distributed confocal laser scanning microscope (CLSM) is its low duty cycle; the laser illuminates only one point at a time. In contrast, the conventional full-field microscope records the images much faster (no scanning is required) and is also more light efficient (no photons are rejected by a pin ...
... distributed confocal laser scanning microscope (CLSM) is its low duty cycle; the laser illuminates only one point at a time. In contrast, the conventional full-field microscope records the images much faster (no scanning is required) and is also more light efficient (no photons are rejected by a pin ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.